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The marker choice: Unexpected resolving power of an unexplored CO1 region for layered DNA barcoding approaches

https://doi.org/10.1371/journal.pone.0174842
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49/49 checkable references clean · checked 2026-07-22

Every reference with a DOI in the deposited reference list resolved to a known work in Crossref or DataCite at the dated check, and none carried a retraction, withdrawal, or removal notice.

7 without a DOI — not checked. A reference deposited without a DOI is never matched by title or guessed at; it stays outside the checked set, and this line discloses that.

The 49 checked references that resolve
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Environmental<scp>DNA</scp>metabarcoding of lake fish communities reflects long‐term data from established survey methods
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Advances in DNA metabarcoding for food and wildlife forensic species identification
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Next‐generation monitoring of aquatic biodiversity using environmental <scp>DNA</scp> metabarcoding
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DNA metabarcoding and the cytochrome <i>c</i> oxidase subunit I marker: not a perfect match
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Testing the potential of a ribosomal 16S marker for DNA metabarcoding of insects
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<i>CO1</i> phylogenies in diploblasts and the ‘Barcoding of Life’— are we sequencing a suboptimal partition?
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Animal mitochondrial DNA recombination
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The ecology of environmental DNA and implications for conservation genetics
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Evolutionary genomics in Metazoa: the mitochondrial DNA as a model system
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Identification of Birds through DNA Barcodes
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Comprehensive DNA barcode coverage of North American birds
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DNA Barcode Libraries Provide Insight into Continental Patterns of Avian Diversification
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The campaign to DNA barcode all fishes, FISH‐BOL
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Identification of Genes Suitable for DNA Barcoding of Morphologically Indistinguishable Korean Halichondriidae Sponges
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Slow mitochondrial DNA sequence evolution in the Anthozoa (Cnidaria)
resolves10.1016/S1055-7903(03)00140-4
Testing the utility of partial COI sequences for phylogenetic estimates of gastropod relationships
resolves10.1111/1755-0998.12138
Redesign of <scp>PCR</scp> primers for mitochondrial cytochrome <i>c</i> oxidase subunit <scp>I</scp> for marine invertebrates and application in all‐taxa biotic surveys
resolves10.1111/1755-0998.12135
<i>Cytochrome c oxidase I</i> primers for corbiculate bees: <scp>DNA</scp> barcode and mini‐barcode
resolves10.1016/j.ympev.2006.12.005
Patterns of evolution of mitochondrial cytochrome c oxidase I and II DNA and implications for DNA barcoding
resolves10.1111/j.1755-0998.2011.03090.x
Universal COI primers for DNA barcoding amphibians
resolves10.1111/1755-0998.12125
The potential of distance‐based thresholds and character‐based<scp>DNA</scp>barcoding for defining problematic taxonomic entities by<scp>CO</scp>1 and<scp>ND</scp>1
resolves10.1080/13887890.2007.9748286
The<i>Pseudagrion</i>split: molecular phylogeny confirms the morphological and ecological dichotomy of Africa's most diverse genus of Odonata (Coenagrionidae)
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resolves10.1098/rspb.2007.1290
Character-based DNA barcoding allows discrimination of genera, species and populations in Odonata
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Allozyme and mitochondrial DNA variability within the New Zealand damselfly genera <i>Xanthocnemis, Austrolestes</i> , and <i>Ischnura</i> (Odonata)
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Applications of random amplified polymorphic DNA (RAPD) in molecular ecology
resolves10.1093/nar/gkh340
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An automated phylogenetic key for classifying homeoboxes
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Characteristic attributes in cancer microarrays
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Some ‘ant’swers: Application of a layered barcode approach to problems in ant taxonomy
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The recognition and evaluation of homoplasy in primate and human evolution
resolves10.1111/1755-0998.12363
<scp>DNA</scp> barcoding largely supports 250 years of classical taxonomy: identifications for <scp>C</scp>entral <scp>E</scp>uropean bees (<scp>H</scp>ymenoptera, <scp>A</scp>poidea <i>partim</i>)
resolves10.1111/1755-0998.12440
Identification of<scp>N</scp>orth<scp>S</scp>ea molluscs with<scp>DNA</scp>barcoding
resolves10.1111/1755-0998.12344
Barcoding Neotropical birds: assessing the impact of nonmonophyly in a highly diverse group
resolves10.1098/rspb.2007.1035
Limited performance of DNA barcoding in a diverse community of tropical butterflies
resolves10.1007/s00239-008-9069-5
Slow Mitochondrial COI Sequence Evolution at the Base of the Metazoan Tree and Its Implications for DNA Barcoding
resolves10.1080/10635150600969864
DNA Barcoding and Taxonomy in Diptera: A Tale of High Intraspecific Variability and Low Identification Success
resolves10.1080/10635150490503053
Multiple Data Sets, High Homoplasy, and the Phylogeny of Softshell Turtles (Testudines: Trionychidae)
resolves10.1016/j.ympev.2013.08.001
Hide and seek: Placing and finding an optimal tree for thousands of homoplasy-rich sequences
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Insect Mitochondrial Genomics: Implications for Evolution and Phylogeny
The 7 references without a DOI — listed, not checked
no DOI — not checkedHigh-throughput metabarcoding of eukaryotic diversity for environmental monitoring of offshore oil drilling activities
no DOI — not checkedMolecular markers, natural history, and evolution
no DOI — not checkedInsect Molecular Genetics
no DOI — not checkedDNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates
no DOI — not checkedThe present role and future promise of conservation genetics for forest Odonates
no DOI — not checkedPAUP*: Phylogenetic Analysis Using Parsimony (*and Other Methods) 4.0 Beta
no DOI — not checkedTesting the potential of DNA barcoding in vertebrate radiations: the case of the littoral cichlids (Pisces, Perciformes, Cichlidae) from Lake Tanganyika
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