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The Rickettsia Surface Protein OmpB is Critical for Virulence and Evasion of Autophagy

https://doi.org/10.2139/ssrn.3325031
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38/38 checkable references clean · checked 2026-08-19

Every reference with a DOI in the deposited reference list resolved to a known work in Crossref or DataCite at the dated check, and none carried a retraction, withdrawal, or removal notice.

21 without a DOI — not checked. A reference deposited without a DOI is never matched by title or guessed at; it stays outside the checked set, and this line discloses that.

The 38 checked references that resolve
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Avoidance of Autophagy Mediated by PlcA or ActA Is Required for Listeria monocytogenes Growth in Macrophages
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Entrapment of Intracytosolic Bacteria by Septin Cage-like Structures
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p62 and NDP52 Proteins Target Intracytosolic Shigella and Listeria to Different Autophagy Pathways
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Less label, more free: Approaches in label‐free quantitative mass spectrometry
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Targeted Knockout of the Rickettsia rickettsii OmpA Surface Antigen Does Not Diminish Virulence in a Mammalian Model System
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Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii
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Escape of Intracellular <i>Shigella</i> from Autophagy
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Quantitative Proteomics Reveal a Feedforward Model for Mitochondrial PARKIN Translocation and Ubiquitin Chain Synthesis
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<i>Rickettsia parkeri</i>Rickettsiosis and Its Clinical Distinction from Rocky Mountain Spotted Fever
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Expanding the host cell ubiquitylation machinery targeting cytosolic Salmonella
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Differential activity of Rickettsia rickettsii ompA and ompB promoter regions in a heterologous reporter gene system
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The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases
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Self and Nonself: How Autophagy Targets Mitochondria and Bacteria
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Rickettsia Actin-Based Motility Occurs in Distinct Phases Mediated by Different Actin Nucleators
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Nonselective Persistence of a Rickettsia conorii Extrachromosomal Plasmid during Mammalian Infection
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The<i>Rickettsia conorii</i>Autotransporter Protein Sca1 Promotes Adherence to Nonphagocytic Mammalian Cells
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The Rickettsial OmpB β-Peptide of Rickettsia conorii Is Sufficient To Facilitate Factor H-Mediated Serum Resistance
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Corporate integrity: rethinking organizational ethics and leadership
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resolves10.1038/nmeth.2089
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Effects of Traveling Wave Ion Mobility Separation on Data Independent Acquisition in Proteomics Studies
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Rickettsia rickettsii-induced cellular injury of human vascular endothelium in vitro
resolves10.1038/nrmicro1866
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Expression of an Epitope-Tagged Virulence Protein in Rickettsia parkeri Using Transposon Insertion
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The crystal structure of the catalytic domain of a eukaryotic guanylate cyclase
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Verkündungen im Verkehrsblatt
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Supplementary file 6. Survival of CF512 worms on pathogenic bacteria or after UV radiation (related to Figure 6)
The 21 references without a DOI — listed, not checked
no DOI — not checkedref13
no DOI — not checkedQuantitative proteomics reveal a feedforward 630 mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis
no DOI — not checkedRickettsia parkeri 635 rickettsiosis and its clinical distinction from Rocky Mountain spotted fever
no DOI — not checkedExternal layers of 678 Rickettsia prowazekii and Rickettsia rickettsii: occurrence of a slime layer
no DOI — not checkedAssembly and 717 Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control
no DOI — not checkedref45
no DOI — not checked). 6xHis-SUMO-OmpB aa36-218 was expressed in E. coli strain BL21 codon plus RIL-Cam r 1062 (DE3) (QB3 Macrolab, UC Berkeley) by induction with 0.1 mM isopropyl-?-D-thio-galactoside 1063 (IPTG) for 16 h at 16�C. Bacterial pellets were resuspended in lysis buffer (50 mM NaH2PO4, pH 1064 8.0, 300 mM NaCl, 10 mM imidazole) and stored at -80�C. For protein purification
no DOI — not checkedQiagen, 1018244) 1067 and bound proteins were eluted with a step gradient of 100, 250 and 500 mM imidazole (in lysis 1068 buffer, pH adjusted to 8.0). Fractions were analyzed by SDS-PAGE and those with the highest 1069 concentrations of 6xHis-SUMO-OmpB 36-218 were pooled prior to desalting
no DOI — not checkedref53
no DOI — not checkedand immunization 1073 was carried out according to their 91-d protocol. To affinity-purify anti-OmpB antibodies, ompB aa36-1074 218 was subcloned into plasmid pET1M, which encodes N-terminal 6xHis and maltose-binding 1075 protein (MBP) tags. 6xHis-MBP-OmpB aa36-218 was expressed and
no DOI — not checkedBound proteins were eluted 1079 in column buffer lacking EDTA and containing 10 mM maltose. 500?g of purified protein was 1080 coupled to NHS-activated Sepharose 4 Fast Flow resin (GE-Healthcare, 17090601) in ligand-1081 coupling buffer (200 mM NaHCO3 pH 8.3, 500 mM NaCl) overnight at 4�C. Resin was incubated 1082 with 10 mL of anti-OmpB serum
no DOI — not checkedselection-1) (starting MOI: 0.002) as measured by genomic equivalents using qPCR 1295 from an experiment performed in biological triplicates (statistical comparisons with parental 1296 ompB::tn for each time point were performed using an unpaired Student
no DOI — not checked(bp) position 1299 1045462 in the R. parkeri genome); ompB STOP ::tn (with an additional 23-bp deletion at position 1300 1045835-1045857); ompB PROM ::tn (with an additional 17-bp insertion in the promoter region of 1301 ompB at position 1046683); and MC1_RS02370 STOP , ompB::tn (with an additional two deletions, 1302 of 112 at position 461273-461384 and 125 bp at position 461425-461549 in a gene encoding a 1303 predicted glycosyltransferase (MC1_RS02370), leading to a premature stop codon). (D) Western 1304 blot of 10 6 30%-purified WT, ompB::tn and ompB suppressor strains probed for OmpB (anti-OmpB 1305 antibody
no DOI — not checkedref59
no DOI — not checkedref60
no DOI — not checked001). (F) Western blot of 10 6 30%-purified WT
no DOI — not checkedOmpA/Sca2/17-kDa antigen, are not required for R. parkeri 1313 internalization, and OmpB is not required for actin mobilization or bacterial membrane 1314 integrity
no DOI — not checkedData are mean � 1316 SEM from at least two independent experiments (means were not significantly different by an 1317 unpaired Student's t-test). (B and C) Quantification of the percentage of bacteria with early (B) 1318 actin tails or (C) actin clouds. Data are mean � SEM from two independent experiments (for each 1319 time point, means were not significantly different by an unpaired
no DOI — not checkedref64
no DOI — not checkedref65
no DOI — not checkedref66
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