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Variations in the Extraction and Clean-Up Efficiency of DNA Isolates During Anaerobic Digestion of Wastewater Sludge at Mesophilic and Thermophilic Temperatures

https://doi.org/10.2139/ssrn.4012850
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30/30 checkable references clean · checked 2026-07-23

Every reference with a DOI in the deposited reference list resolved to a known work in Crossref or DataCite at the dated check, and none carried a retraction, withdrawal, or removal notice.

11 without a DOI — not checked. A reference deposited without a DOI is never matched by title or guessed at; it stays outside the checked set, and this line discloses that.

The 30 checked references that resolve
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Assessment of Commercial DNA Cleanup Kits for Elimination of Real-Time PCR Inhibitors in the Detection of Cyclospora cayetanensis in Cilantro
resolves10.1128/AEM.01383-14
Agreement between <i>amoA</i> Gene-Specific Quantitative PCR and Fluorescence <i>In Situ</i> Hybridization in the Measurement of Ammonia-Oxidizing Bacteria in Activated Sludge
resolves10.1016/j.syapm.2009.11.004
Influence of DNA isolation on Q-PCR-based quantification of methanogenic Archaea in biogas fermenters
resolves10.1007/s00253-010-2686-0
Improving the recovery of qPCR-grade DNA from sludge and sediment
resolves10.1016/j.jclepro.2019.118195
Effects of waste rusted iron shavings on enhancing anaerobic digestion of food wastes and municipal sludge
resolves10.1016/0003-2697(68)90307-2
Spectrophotometric determination of microgram quantities of protein without nucleic acid interference
resolves10.2166/wst.1986.0295
A Mathematical Model for the Anaerobic Digestion Process Applied to a Mixture of Night Soil and Septic Tank Sludge
resolves10.2166/wh.2018.108
Assessing high-throughput environmental DNA extraction methods for meta-barcode characterization of aquatic microbial communities
resolves10.1080/09593330.2019.1649467
Start-up of oxygen-limited autotrophic partial nitrification-anammox process for treatment of nitrite-free wastewater in a single-stage hybrid bioreactor
resolves10.1007/s13213-013-0704-z
Metagenomic analyses reveal phylogenetic diversity of carboxypeptidase gene sequences in activated sludge of a wastewater treatment plant in Shanghai, China
resolves10.1016/j.biortech.2014.07.065
Improving the amenability of municipal waste activated sludge for biological pretreatment by phase-separated sludge disintegration method
resolves10.1007/s10532-007-9125-7
Correlation of microbial mass with ATP and DNA concentrations in acidogenesis of whey permeate
resolves10.1016/j.mimet.2017.10.007
Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples
resolves10.1016/j.micres.2007.07.004
Ability of DNA content and DGGE analysis to reflect the performance condition of an anaerobic biowaste fermenter
resolves10.1016/S0043-1354(03)00171-4
Mechanisms of floc destruction during anaerobic and aerobic digestion and the effect on conditioning and dewatering of biosolids
resolves10.1016/S0022-2860(03)00103-0
Spectroscopic study of the humification process during sewage sludge treatment
resolves10.1016/j.mcp.2009.11.001
A comparison of six methods for genomic DNA extraction suitable for PCR-based genotyping applications using ovine milk samples
resolves10.1186/s13104-015-1407-2
Effects of freezing storage on the DNA extraction and microbial evaluation from anaerobic digested sludges
resolves10.1002/0471649643
Environmental Organic Chemistry
resolves10.1016/0048-9697(89)90120-4
Spectroscopic and compositional comparative characterization of I.H.S.S. reference and standard fulvic and humic acids of various origin
resolves10.1016/S1001-0742(11)61031-0
Enhanced anaerobic digestion and sludge dewaterability by alkaline pretreatment and its mechanism
resolves10.1002/bit.260411111
Anaerobic waste‐activated sludge digestion–a bioconversion mechanism and kinetic model
resolves10.1016/j.biortech.2020.123510
Determining the composition of bacterial community and relative abundance of specific antibiotics resistance genes via thermophilic anaerobic digestion of sewage sludge
resolves10.1128/aem.59.8.2657-2665.1993
Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast
resolves10.1016/j.biortech.2017.03.104
Occurrence of antibiotic resistance genes and mobile genetic elements in enterococci and genomic DNA during anaerobic digestion of pharmaceutical waste sludge with different pretreatments
resolves10.1007/s00253-010-2770-5
Bacterial community analysis of activated sludge: an evaluation of four commonly used DNA extraction methods
resolves10.1016/j.jhazmat.2020.124428
Quantifying the occurrence and transformation potential of extracellular polymeric substances (EPS)-associated antibiotic resistance genes in activated sludge
resolves10.1016/j.jchromb.2007.03.009
Comparison of strategies for the isolation of PCR-compatible, genomic DNA from a municipal biogas plants
resolves10.1016/j.biortech.2020.123554
Impacts of Cu and Zn on the performance, microbial community dynamics and resistance genes variations during mesophilic and thermophilic anaerobic digestion of swine manure
resolves10.1016/j.scitotenv.2020.138248
Evolutions of antibiotic resistance genes (ARGs), class 1 integron-integrase (intI1) and potential hosts of ARGs during sludge anaerobic digestion with the iron nanoparticles addition
The 11 references without a DOI — listed, not checked
no DOI — not checkedref2
no DOI — not checkedFree-floating extracellular DNA: Systematic profiling of mobile genetic elements and antibiotic resistance from wastewater
no DOI — not checkedUnveiling the biotic and abiotic mechanisms of solids concentration affecting hydrolysis and acidogenesis during sludge thermophilic anaerobic fermentation
no DOI — not checkedEffects of sample preservation and DNA extraction on enumeration of antibiotic resistance genes in wastewater
no DOI — not checkedEffect of nitrite addition on the two-phase anaerobic digestion of waste activated sludge: Optimization of the acidogenic phase and influence mechanisms
no DOI — not checkedref19
no DOI — not checkedThe environmental contribution to the dissemination of carbapenem and (fluoro)quinolone resistance genes by discharged and reused wastewater effluents: The role of cellular and extracellular DNA
no DOI — not checkedBiases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples
no DOI — not checkedref34
no DOI — not checkedBacterial diversity of active sludge in wastewater treatment plant
no DOI — not checkedRedistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beads
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