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Evaluation of Pre-Analytical and Analytical Methods for Detecting SARS-CoV-2 in Municipal Wastewater Samples in Northern Italy

https://doi.org/10.3390/w14050833
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30/30 checkable references clean · checked 2026-07-22

Every reference with a DOI in the deposited reference list resolved to a known work in Crossref or DataCite at the dated check, and none carried a retraction, withdrawal, or removal notice.

3 without a DOI — not checked. A reference deposited without a DOI is never matched by title or guessed at; it stays outside the checked set, and this line discloses that.

The 30 checked references that resolve
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A Novel Coronavirus from Patients with Pneumonia in China, 2019
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Surveillance of SARS-CoV-2 RNA in wastewater: Methods optimization and quality control are crucial for generating reliable public health information
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Sewage analysis as a tool for the COVID-19 pandemic response and management: the urgent need for optimised protocols for SARS-CoV-2 detection and quantification
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Implementation of environmental surveillance for SARS-CoV-2 virus to support public health decisions: Opportunities and challenges
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Measurement of SARS-CoV-2 RNA in wastewater tracks community infection dynamics
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Tracking COVID-19 with wastewater
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Long-term monitoring of SARS-CoV-2 RNA in wastewater of the Frankfurt metropolitan area in Southern Germany
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Analytical methodologies for the detection of SARS-CoV-2 in wastewater: Protocols and future perspectives
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A comparison of SARS-CoV-2 wastewater concentration methods for environmental surveillance
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First detection of SARS-CoV-2 in untreated wastewaters in Italy
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Wastewater surveillance of SARS-CoV-2 across 40 U.S. states from February to June 2020
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SARS-CoV-2 RNA in urban wastewater samples to monitor the COVID-19 pandemic in Lombardy, Italy (March–June 2020)
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A review on recent progress in the detection methods and prevalence of human enteric viruses in water
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Monitoring human enteric viruses in wastewater and relevance to infections encountered in the clinical setting: a one-year experiment in central France, 2014 to 2015
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Surveillance of poliomyelitis in Northern Italy: Results of acute flaccid paralysis surveillance and environmental surveillance, 2012–2015
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Detection of Pathogenic Viruses in Sewage Provided Early Warnings of Hepatitis A Virus and Norovirus Outbreaks
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Sporadic Isolation of Sabin-Like Polioviruses and High-Level Detection of Non-Polio Enteroviruses during Sewage Surveillance in Seven Italian Cities, after Several Years of Inactivated Poliovirus Vaccination
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Molecular Characterization and Phylogenetic Analysis of Enteroviruses and Hepatitis A Viruses in Sewage Samples, Northern Italy, 2016
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First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: A proof of concept for the wastewater surveillance of COVID-19 in the community
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SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area
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First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan
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First detection of SARS-CoV-2 RNA in wastewater in North America: A study in Louisiana, USA
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Detection of SARS-CoV-2 in raw and treated wastewater in Germany – Suitability for COVID-19 surveillance and potential transmission risks
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Comparison of five polyethylene glycol precipitation procedures for the RT-qPCR based recovery of murine hepatitis virus, bacteriophage phi6, and pepper mild mottle virus as a surrogate for SARS-CoV-2 from wastewater
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Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater
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Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection
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Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
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The 3 references without a DOI — listed, not checked
no DOI — not checkedWord Health Organization (WHO) (2022, March 05). Contributions of the Polio Network to COVID-19 Response. Available online: https://apps.who.int/iris/bitstream/handle/10665/336261/9789240011533-eng.pdf?sequence=1&isAllowed=y.
no DOI — not checkedRegione Lombardia (2022, March 05). Regione Lombardia, Delibera N.4127—Accordo di Collaborazione con il Ministero Della Salute-Centro Nazionale per la Prevenzione e il Controllo delle Malattie e Convenzioni con l’Istituto Superiore di Sanità e l’ATS Milano Città Metropolitana per la Realizzazione del Progetto CCM 2020—Area Progettuale “Epidemiologia delle Acque Reflue: Implementazione del Sistema di Sorveglianza per L’identificazione Precoce di Agenti Patogeni, con Particolare Riferimento al SARS-CoV2”. Available online: https://www.dati.lombardia.it/api/views/q639-trxu/rows.xml?accessType=DOWNLOAD.
no DOI — not checkedWord Health Organization (WHO) (2022, March 05). WHO Guidelines for Environmental Surveillance of Poliovirus Protocol—WHO/V&B/03.03. Available online: https://polioeradication.org/wp-content/uploads/2016/07/WHO_V-B_03.03_eng.pdf.
What this badge says. CiteStamped means the CHECKABLE references of this work were clean at the dated check: each resolved to a known work in a public registry, and none carried a retraction notice at that time. It says nothing about the quality, findings, or importance of the work itself, and nothing about references deposited without a DOI.

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