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DNA metabarcoding of Neotropical ichthyoplankton: Enabling high accuracy with lower cost

https://doi.org/10.3897/mbmg.3.35060
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43/43 checkable references clean · checked 2026-07-22

Every reference with a DOI in the deposited reference list resolved to a known work in Crossref or DataCite at the dated check, and none carried a retraction, withdrawal, or removal notice.

1 without a DOI — not checked. A reference deposited without a DOI is never matched by title or guessed at; it stays outside the checked set, and this line discloses that.

The 43 checked references that resolve
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DNA barcoding as a useful tool for identifying non-native species of freshwater ichthyoplankton in the neotropics
resolves10.1016/S0022-2836(05)80360-2
Basic local alignment search tool
resolves10.1002/ece3.1321
<scp>DNA</scp> barcoding reveals novel insights into pterygophagy and prey selection in distichodontid fishes (Characiformes: Distichodontidae)
resolves10.1002/ece3.4934
Metabarcoding for stomach‐content analyses of Pygmy devil ray (<i>Mobula kuhlii cf. eregoodootenkee)</i>: Comparing tissue and ethanol preservative‐derived DNA
resolves10.1007/s10641-004-0098-z
Identification of spawning sites and natural nurseries of fishes in the upper Paraná River, Brazil
resolves10.1111/jfb.12707
<scp>DNA</scp> barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco
resolves10.1007/s10641-004-3795-3
Larval fish assemblage in the Baía River (Mato Grosso do Sul State, Brazil): temporal and spatial patterns
resolves10.1093/bioinformatics/btu170
Trimmomatic: a flexible trimmer for Illumina sequence data
resolves10.1111/1755-0998.12428
<scp>obitools</scp>: a <scp>unix</scp>‐inspired software package for <scp>DNA</scp> metabarcoding
resolves10.1038/srep41948
High-throughput sequencing of multiple amplicons for barcoding and integrative taxonomy
resolves10.1111/1755-0998.12770
Disparities in second‐generation <scp>DNA</scp> metabarcoding results exposed with accessible and repeatable workflows
resolves10.1371/journal.pone.0122763
Use of Droplet Digital PCR for Estimation of Fish Abundance and Biomass in Environmental DNA Surveys
resolves10.1093/nar/gkh340
MUSCLE: multiple sequence alignment with high accuracy and high throughput
resolves10.3389/fenvs.2017.00011
Validation and Development of COI Metabarcoding Primers for Freshwater Macroinvertebrate Bioassessment
resolves10.1016/j.fishres.2017.09.013
Freshwater fisheries assessment using environmental DNA: A primer on the method, its potential, and shortcomings as a conservation tool
resolves10.1111/1755-0998.12433
Quantification of mesocosm fish and amphibian species diversity via environmental <scp>DNA</scp> metabarcoding
resolves10.1111/1755-0998.12338
Replication levels, false presences and the estimation of the presence/absence from <scp>eDNA</scp> metabarcoding data
resolves10.1111/1755-0998.12508
How to limit false positives in environmental <scp>DNA</scp> and metabarcoding?
resolves10.1111/1755-0998.12385
<scp>DNA</scp> barcoding of freshwater ichthyoplankton in the Neotropics as a tool for ecological monitoring
resolves10.1111/jfb.13294
Application of <scp>DNA</scp> metabarcoding on faeces to identify European catfish <i>Silurus glanis</i> diet
resolves10.1111/mec.13660
Environmental<scp>DNA</scp>metabarcoding of lake fish communities reflects long‐term data from established survey methods
resolves10.1111/faf.12286
The sceptical optimist: challenges and perspectives for the application of environmental <scp>DNA</scp> in marine fisheries
resolves10.1111/j.1471-8286.2006.01428.x
An inexpensive, automation‐friendly protocol for recovering high‐quality DNA
resolves10.1093/bioinformatics/bts199
Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data
resolves10.3389/fmars.2016.00283
Genetic and Manual Survey Methods Yield Different and Complementary Views of an Ecosystem
resolves10.1007/BF01731581
A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences
resolves10.1093/molbev/msw054
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
resolves10.1371/journal.pone.0170009
DNA Metabarcoding of Amazonian Ichthyoplankton Swarms
resolves10.1371/journal.pone.0202976
Metabarcoding by capture using a single COI probe (MCSP) to identify and quantify fish species in ichthyoplankton swarms
resolves10.1371/journal.pone.0114639
The Release Rate of Environmental DNA from Juvenile and Adult Fish
resolves10.1111/fwb.13094
Comparing local‐ and regional‐scale estimations of the diversity of stream fish using <scp>eDNA</scp> metabarcoding and conventional observation methods
resolves10.1186/1471-2156-14-20
Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna?
resolves10.1111/j.1471-8286.2007.01678.x
<scp>bold</scp> : The Barcode of Life Data System (http://www.barcodinglife.org)
resolves10.1007/s10641-011-9955-3
Spatial and temporal variation of the ichthyoplankton in a subtropical river in Brazil
resolves10.1002/edn3.14
Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river
resolves10.1038/srep09687
Massively parallel multiplex DNA sequencing for specimen identification using an Illumina MiSeq platform
resolves10.1007/s10750-016-2953-x
Fish larvae diversity in a conservation area of a neotropical floodplain: influence of temporal and spatial scales
resolves10.1590/s2179-975x10216
Importance of dam-free stretches for fish reproduction: the last remnant in the Upper Paraná River
resolves10.1002/rra.2755
Importance of Reservoir Tributaries to Spawning of Migratory Fish in the Upper Paraná River
resolves10.1371/journal.pone.0035868
Estimation of Fish Biomass Using Environmental DNA
resolves10.1111/mec.13428
Next‐generation monitoring of aquatic biodiversity using environmental <scp>DNA</scp> metabarcoding
resolves10.1098/rstb.2005.1716
DNA barcoding Australia's fish species
resolves10.1038/srep40368
Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
The 1 reference without a DOI — listed, not checked
no DOI — not checked35060_B3
What this badge says. CiteStamped means the CHECKABLE references of this work were clean at the dated check: each resolved to a known work in a public registry, and none carried a retraction notice at that time. It says nothing about the quality, findings, or importance of the work itself, and nothing about references deposited without a DOI.

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