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Explore or exploit? A model‐based screening strategy for PETase secretion by Corynebacterium glutamicum

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/biot.201800428openalex-snapshot

Less Sacrifice, More Insight: Repeated Low‐Volume Sampling of Microbioreactor Cultivations Enables Accelerated Deep Phenotyping of Microbial Strain Libraries

inferredcites →https://doi.org/10.1002/bit.25103openalex-snapshot

Beyond growth rate 0.6: What drives Corynebacterium glutamicum to higher growth rates in defined medium

inferredcites →https://doi.org/10.1002/elsc.201200021openalex-snapshot

Consistent development of bioprocesses from microliter cultures to the industrial scale

inferredcites →https://doi.org/10.1002/elsc.202100108openalex-snapshot

bletl ‐ A Python package for integrating BioLector microcultivation devices in the Design‐Build‐Test‐Learn cycle

inferredcites →https://doi.org/10.1007/978-3-642-29857-8openalex-snapshot

Corynebacterium glutamicum : biology and biotechnology

inferredcites →https://doi.org/10.1007/978-3-642-29857-8_13openalex-snapshot

Protein Secretion Systems of Corynebacterium glutamicum

inferredcites →https://doi.org/10.1007/s00253-019-09717-yopenalex-snapshot

Current knowledge on enzymatic PET degradation and its possible application to waste stream management and other fields

inferredcites →https://doi.org/10.1007/s00253-022-12017-7openalex-snapshot

Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation

inferredcites →https://doi.org/10.1007/s002530051557openalex-snapshot

A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA

inferredcites →https://doi.org/10.1007/s10994-021-05995-8openalex-snapshot

IntelligentPooling: practical Thompson sampling for mHealth

inferredcites →https://doi.org/10.1016/0378-1119(85)90140-4openalex-snapshot

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

inferredcites →https://doi.org/10.1016/b978-0-12-821477-0.00006-4openalex-snapshot

Corynebacterium glutamicum as a robust microbial factory for production of value-added proteins and small molecules: fundamentals and applications

inferredcites →https://doi.org/10.1016/j.jbiotec.2005.06.015openalex-snapshot

Enzymatic surface modification of poly(ethylene terephthalate)

inferredcites →https://doi.org/10.1016/j.jbiotec.2017.02.023openalex-snapshot

Beyond amino acids: Use of the Corynebacterium glutamicum cell factory for the secretion of heterologous proteins

inferredcites →https://doi.org/10.1016/j.jmb.2006.07.034openalex-snapshot

Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria

inferredcites →https://doi.org/10.1016/j.plantsci.2018.03.035openalex-snapshot

From plant metabolic engineering to plant synthetic biology: The evolution of the design/build/test/learn cycle

inferredcites →https://doi.org/10.1016/j.ymben.2021.12.006openalex-snapshot

The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future

inferredcites →https://doi.org/10.1016/s0168-6496(01)00134-9openalex-snapshot

Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops

inferredcites →https://doi.org/10.1021/acscatal.8b05171openalex-snapshot

Biodegradation of PET: Current Status and Application Aspects

inferredcites →https://doi.org/10.1021/bi401561popenalex-snapshot

Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase

inferredcites →https://doi.org/10.1038/s41586-020-2149-4openalex-snapshot

An engineered PET depolymerase to break down and recycle plastic bottles

inferredcites →https://doi.org/10.1038/s41586-020-2649-2openalex-snapshot

Array programming with NumPy

inferredcites →https://doi.org/10.1038/s41592-019-0686-2openalex-snapshot

SciPy 1.0: fundamental algorithms for scientific computing in Python

inferredcites →https://doi.org/10.1038/s42003-018-0076-9openalex-snapshot

An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals

inferredcites →https://doi.org/10.1038/s43586-020-00001-2openalex-snapshot

Bayesian statistics and modelling

inferredcites →https://doi.org/10.1093/biomet/25.3-4.285openalex-snapshot

ON THE LIKELIHOOD THAT ONE UNKNOWN PROBABILITY EXCEEDS ANOTHER IN VIEW OF THE EVIDENCE OF TWO SAMPLES

inferredcites →https://doi.org/10.1109/mcse.2007.55openalex-snapshot

Matplotlib: A 2D Graphics Environment

inferredcites →https://doi.org/10.1126/scienceopenalex-snapshot

Science

inferredcites →https://doi.org/10.1126/science.aad6359openalex-snapshot

A bacterium that degrades and assimilates poly(ethylene terephthalate)

inferredcites →https://doi.org/10.1128/aem.01146-10openalex-snapshot

Optimization of Protease Secretion in Bacillus subtilis and Bacillus licheniformis by Screening of Homologous and Heterologous Signal Peptides

inferredcites →https://doi.org/10.1128/aem.06725-11openalex-snapshot

Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

inferredcites →https://doi.org/10.1128/jb.138.3.663-670.1979openalex-snapshot

Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens

inferredcites →https://doi.org/10.1145/3097983.3098184openalex-snapshot

An Efficient Bandit Algorithm for Realtime Multivariate Optimization

inferredcites →https://doi.org/10.1186/1475-2859-11-144openalex-snapshot

An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform

inferredcites →https://doi.org/10.1186/s12934-016-0604-6openalex-snapshot

Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum

inferredcites →https://doi.org/10.1201/9780429258411openalex-snapshot

Bayesian Data Analysis

inferredcites →https://doi.org/10.1371/journal.pcbi.1009223openalex-snapshot

Bayesian calibration, process modeling and uncertainty quantification in biotechnology

inferredcites →https://doi.org/10.1561/2200000070openalex-snapshot

A Tutorial on Thompson Sampling

inferredcites →https://doi.org/10.17863/cam.64134openalex-snapshot

SciPy 1.0: fundamental algorithms for scientific computing in Python

inferredcites →https://doi.org/10.21105/joss.01143openalex-snapshot

ArviZ a unified library for exploratory analysis of Bayesian models in Python

inferredcites →https://doi.org/10.2323/jgam.3.193openalex-snapshot

STUDIES ON THE AMINO ACID FERMENTATION

inferredcites →https://doi.org/10.25080/majora-92bf1922-00aopenalex-snapshot

Data Structures for Statistical Computing in Python

inferredcites →https://doi.org/10.3389/fmicb.2020.00114openalex-snapshot

A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri – Structural and Functional Insights

inferredcites →https://doi.org/10.48550/arxiv.1111.1797openalex-snapshot

Analysis of Thompson Sampling for the multi-armed bandit problem

inferredcites →https://doi.org/10.48550/arxiv.1111.4246openalex-snapshot

The No-U-Turn Sampler: Adaptively Setting Path Lengths in Hamiltonian Monte Carlo

inferredcites →https://doi.org/10.48550/arxiv.1703.06240openalex-snapshot

Multi-fidelity Bayesian Optimisation with Continuous Approximations

inferredcites →https://doi.org/10.48550/arxiv.1706.01825openalex-snapshot

Parallel and Distributed Thompson Sampling for Large-scale Accelerated Exploration of Chemical Space

inferredcites →https://doi.org/10.5281//zenodoopenalex-snapshot

Analyzing nitrogen dioxide to nitrogen oxide scaling factors for data-driven satellite-based emission estimation methods: A case study of Matimba/Medupi power stations in South Africa

inferredcites →https://doi.org/10.5281/zenodo.3509134openalex-snapshot

pandas-dev/pandas: Pandas

inferredcites →https://doi.org/10.5281/zenodo.3831380openalex-snapshot

michaelosthege/pyrff: v1.0.1

inferredcites →https://doi.org/10.5281/zenodo.4127012openalex-snapshot

JuBiotech/calibr8: PyMc v4.0.0b2 compatibility

inferredcites →https://doi.org/10.5281/zenodo.4697605openalex-snapshot

JuBiotech/robotools: v1.0.0

inferredcites →https://doi.org/10.5281/zenodo.5101434openalex-snapshot

JuBiotech/bletl: v.1.6.2

inferredcites →https://doi.org/10.5281/zenodo.5850149openalex-snapshot

pymc-devs/pymc: v4.0.0b2

inferredcites →https://doi.org/10.5281/zenodo.6908128openalex-snapshot

JuBiotech/petase-ts-paper: 2022-07-26

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← backgroundhttps://doi.org/10.5281/zenodo.13985585zenodo-depositor-declared

inferred← citeshttps://doi.org/10.1002/bit.28960openalex-snapshot

Bayesian Optimization in Bioprocess Engineering—Where Do We Stand Today?

inferred← citeshttps://doi.org/10.1002/bit.70129openalex-snapshot

A Guide to Bayesian Optimization in Bioprocess Engineering

inferred← citeshttps://doi.org/10.1002/btpr.3495openalex-snapshot

Quantification of nisin concentration from fluorescence‐based antimicrobial activity assay using Bayesian calibration

inferred← citeshttps://doi.org/10.1002/cctc.202400777openalex-snapshot

Avoiding Replicates in Biocatalysis Experiments: Machine Learning for Enzyme Cascade Optimization

inferred← citeshttps://doi.org/10.1007/978-1-0716-4779-0_34openalex-snapshot

Best Practices for Protein Expression in Pichia pastoris Combining Design of Experiments with an Automated Platform for Parallelized Cultivation in Small-Scale Stirred Tank Bioreactors

inferred← citeshttps://doi.org/10.1007/978-3-032-17514-4_4openalex-snapshot

Parallelized Bioprocess Development in Small-Scale Bioreactors

inferred← citeshttps://doi.org/10.1016/j.biortech.2025.133024openalex-snapshot

Engineering MtrAB two-component system enhances protein and glutamate export in corynebacterium glutamicum through cell wall remodeling

inferred← citeshttps://doi.org/10.1016/j.copbio.2025.103382openalex-snapshot

Building trust in automated experimentation: uncertainty quantification in the era of high-throughput biolabs

inferred← citeshttps://doi.org/10.1016/j.envadv.2024.100518openalex-snapshot

Differentiation of bacterial communities on five common plastics after six days of exposure to Caribbean coastal waters

inferred← citeshttps://doi.org/10.1016/j.marpolbul.2025.117787openalex-snapshot

Degradation and habitat-dependent colonization of plastics in Caribbean coastal waters and sediments by bacterial communities

inferred← citeshttps://doi.org/10.1111/1758-2229.70169openalex-snapshot

The Effect of Larval Exposure to Plastic Pollution on the Gut Microbiota of the Major Malaria Vector Anopheles arabiensis Patton (Diptera: Culicidae)

inferred← citeshttps://doi.org/10.1186/s12934-023-02140-zopenalex-snapshot

From frozen cell bank to product assay: high-throughput strain characterisation for autonomous Design-Build-Test-Learn cycles

inferred← citeshttps://doi.org/10.1186/s12934-024-02319-yopenalex-snapshot

High-throughput screening of catalytically active inclusion bodies using laboratory automation and Bayesian optimization

inferred← citeshttps://doi.org/10.1186/s12934-024-02547-2openalex-snapshot

Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes

inferred← citeshttps://doi.org/10.3389/fmicb.2026.1815563openalex-snapshot

Waste to wealth: circular approaches for microbial pigment production

inferred← citeshttps://doi.org/10.6084/m9.figshare.25284790openalex-snapshot

Additional file 1 of High-throughput screening of catalytically active inclusion bodies using laboratory automation and Bayesian optimization

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