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Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number

https://doi.org/10.1007/s00216-009-2729-5
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Assay for the quantification of intact/fragmented genomic DNA
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Absolute quantitative detection of ABL tyrosine kinase domain point mutations in chronic myeloid leukemia using a novel nanofluidic platform and mutation-specific PCR
inferredcites →https://doi.org/10.1038/nbt1101-1011bopenalex-snapshot
Accuracy in amplification
inferredcites →https://doi.org/10.1038/nmeth1007-869openalex-snapshot
PCR's next frontier
inferredcites →https://doi.org/10.1038/nmeth916openalex-snapshot
Digital quantification using amplified single-molecule detection
inferredcites →https://doi.org/10.1073/pnas.0608512103openalex-snapshot
Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR
inferredcites →https://doi.org/10.1073/pnas.0705765104openalex-snapshot
Digital PCR for the molecular detection of fetal chromosomal aneuploidy
inferredcites →https://doi.org/10.1073/pnas.96.16.9236openalex-snapshot
Digital PCR
inferredcites →https://doi.org/10.1093/nar/gkn455openalex-snapshot
DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight
inferredcites →https://doi.org/10.1093/nar/gkn518openalex-snapshot
Studying copy number variations using a nanofluidic platform
inferredcites →https://doi.org/10.1111/j.2517-6161.1977.tb01615.xopenalex-snapshot
Modelling Spatial Patterns
inferredcites →https://doi.org/10.1186/1471-2164-8-206openalex-snapshot
A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction
inferredcites →https://doi.org/10.1371/journal.pone.0001662openalex-snapshot
High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array
inferredcites →https://doi.org/10.1371/journal.pone.0002876openalex-snapshot
Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device
inferredcites →https://doi.org/10.1373/clinchem.2007.100016openalex-snapshot
Noninvasive Prenatal Diagnosis of Fetal Chromosomal Aneuploidies by Maternal Plasma Nucleic Acid Analysis
inferredcites →https://doi.org/10.1373/clinchem.2008.111385openalex-snapshot
Microfluidics Digital PCR Reveals a Higher than Expected Fraction of Fetal DNA in Maternal Plasma
inferredcites →https://doi.org/10.1586/14737159.4.1.41openalex-snapshot
Principle and applications of digital PCR
inferredcites →https://doi.org/10.18637/jss.v012.i06openalex-snapshot
spatstat : An R Package for Analyzing Spatial Point Patterns
inferredcites →https://doi.org/10.2144/99261st02openalex-snapshot
Enhancement of PCRs by Partial Restriction Digestion of Genomic Templates

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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inferred← citeshttps://doi.org/10.1039/c9ra10733aopenalex-snapshot
Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technology
inferred← citeshttps://doi.org/10.1039/d0lc00641fopenalex-snapshot
Spontaneous droplet generation via surface wetting
inferred← citeshttps://doi.org/10.1039/d3ay01906copenalex-snapshot
Establishment of reference measurement procedure and reference material for Treponema pallidum
inferred← citeshttps://doi.org/10.1055/s-0044-1785537openalex-snapshot
Laboratory-developed Droplet Digital PCR Assay for Quantification of the JAK2V617F Mutation
inferred← citeshttps://doi.org/10.1055/s-014-59419openalex-snapshot
inferred← citeshttps://doi.org/10.1080/07357907.2019.1571079openalex-snapshot
Expression of Circadian Clock Genes in Human Colorectal Cancer Tissues Using Droplet Digital PCR
inferred← citeshttps://doi.org/10.1080/07388551.2022.2037503openalex-snapshot
Current commercial dPCR platforms: technology and market review
inferred← citeshttps://doi.org/10.1080/08905436.2014.996896openalex-snapshot
Recent Developments in Molecular Detection of Food-Borne Disease Bacteria and GMOs
inferred← citeshttps://doi.org/10.1080/10408398.2013.873767openalex-snapshot
Advanced DNA- and Protein-based Methods for the Detection and Investigation of Food Allergens
inferred← citeshttps://doi.org/10.1080/14737159.2021.1860759openalex-snapshot
dPCR application in liquid biopsies: divide and conquer
inferred← citeshttps://doi.org/10.1088/1742-6596/575/1/012038openalex-snapshot
Absolute quantification of Bovine Viral Diarrhea Virus (BVDV) RNA by the digital PCR technique
inferred← citeshttps://doi.org/10.1093/clinchem/hvaa125openalex-snapshot
The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020
inferred← citeshttps://doi.org/10.1093/femspd/ftaa014openalex-snapshot
Carbon metabolism snapshot by ddPCR during the early step of Candida albicans phagocytosis by macrophages
inferred← citeshttps://doi.org/10.1093/nar/gkac682openalex-snapshot
Directed evolution of colE1 plasmid replication compatibility: a fast tractable tunable model for investigating biological orthogonality
inferred← citeshttps://doi.org/10.1093/nar/gks203openalex-snapshot
Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
inferred← citeshttps://doi.org/10.1093/noajnl/vdz030openalex-snapshot
Rapid and ultrasensitive digital PCR (dPCR) profiling of EGFRvIII in tumor cells and tissues
inferred← citeshttps://doi.org/10.1097/mrm.0b013e328364ff68openalex-snapshot
High-throughput digital PCR in a low-cost and practical format introduction
inferred← citeshttps://doi.org/10.1101/2023.09.06.556592openalex-snapshot
Flexible Methods for Standard Error Calculation in digital PCR Experiments
inferred← citeshttps://doi.org/10.1101/2025.05.28.656494openalex-snapshot
Establishing a Digital PCR-Based Reference Measurement Procedure for Monkeypox Virus: An Inter-laboratory Assessment and Standardization Study
inferred← citeshttps://doi.org/10.1109/jsen.2026.3682335openalex-snapshot
Photocurable Polysiloxane-Based Microfluidic Chip for Convenient Sample Partitioning and Digital Nucleic Acid Assay
inferred← citeshttps://doi.org/10.1117/12.2653703openalex-snapshot
POCT detection device based on thermal convection fluorescence PCR amplification
inferred← citeshttps://doi.org/10.1128/9781555818821.ch2.3.2openalex-snapshot
PCR, Real-Time PCR, Digital PCR, and Isothermal Amplification
inferred← citeshttps://doi.org/10.1128/9781555819071.ch4openalex-snapshot
Digital PCR and Its Potential Application to Microbiology
inferred← citeshttps://doi.org/10.1128/aac.00346-24openalex-snapshot
Antimalarial resistance risk in Mozambique detected by a novel quadruplex droplet digital PCR assay
inferred← citeshttps://doi.org/10.1128/cmr.00103-22openalex-snapshot
Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond
inferred← citeshttps://doi.org/10.1128/jcm.02620-12openalex-snapshot
Comparison of Droplet Digital PCR to Real-Time PCR for Quantitative Detection of Cytomegalovirus
inferred← citeshttps://doi.org/10.1155/2013/134675openalex-snapshot
Development of a Novel Reference Plasmid for Accurate Quantification of Genetically Modified Kefeng6 Rice DNA in Food and Feed Samples
inferred← citeshttps://doi.org/10.1155/2015/392872openalex-snapshot
Current and New Approaches in GMO Detection: Challenges and Solutions
inferred← citeshttps://doi.org/10.1155/2017/7515409openalex-snapshot
Quantitative PCR and Digital PCR for Detection ofAscaris lumbricoidesEggs in Reclaimed Water
inferred← citeshttps://doi.org/10.11606/t.3.2012.tde-10052012-113950openalex-snapshot
Desenvolvimento de sistemas Lab-on-a-Chip para análises em biofísica celular.
inferred← citeshttps://doi.org/10.1186/1471-2105-15-283openalex-snapshot
Impact of variance components on reliability of absolute quantification using digital PCR
inferred← citeshttps://doi.org/10.1186/1754-1611-5-6openalex-snapshot
System Integration - A Major Step toward Lab on a Chip
inferred← citeshttps://doi.org/10.1186/s12864-020-6481-8openalex-snapshot
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation
inferred← citeshttps://doi.org/10.1186/s12864-023-09429-8openalex-snapshot
Vibrio-Sequins - dPCR-traceable DNA standards for quantitative genomics of Vibrio spp
inferred← citeshttps://doi.org/10.1186/s12879-016-1696-7openalex-snapshot
The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis
inferred← citeshttps://doi.org/10.1186/s12936-021-03659-5openalex-snapshot
Measurement of gene amplifications related to drug resistance in Plasmodium falciparum using droplet digital PCR
inferred← citeshttps://doi.org/10.1186/s13148-018-0502-3openalex-snapshot
An epigenetic classifier for early stage lung cancer
inferred← citeshttps://doi.org/10.1371/journal.pntd.0007884openalex-snapshot
Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
inferred← citeshttps://doi.org/10.1371/journal.pone.0013004openalex-snapshot
Experimental Generation of SNP Haplotype Signatures in Patients with Sickle Cell Anaemia
inferred← citeshttps://doi.org/10.1371/journal.pone.0058177openalex-snapshot
Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples
inferred← citeshttps://doi.org/10.1371/journal.pone.0062583openalex-snapshot
Quantitative Analysis of Food and Feed Samples with Droplet Digital PCR
inferred← citeshttps://doi.org/10.1371/journal.pone.0075296openalex-snapshot
Evaluation of Digital PCR for Absolute RNA Quantification
inferred← citeshttps://doi.org/10.1371/journal.pone.0147832openalex-snapshot
A Novel High-Throughput Method for Molecular Detection of Human Pathogenic Viruses Using a Nanofluidic Real-Time PCR System
inferred← citeshttps://doi.org/10.1371/journal.pone.0163060openalex-snapshot
Instrument for Real-Time Digital Nucleic Acid Amplification on Custom Microfluidic Devices
inferred← citeshttps://doi.org/10.1371/journal.pone.0169431openalex-snapshot
Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes
inferred← citeshttps://doi.org/10.1371/journal.pone.0179985openalex-snapshot
Nanofluidic digital PCR for the quantification of Norovirus for water quality assessment
inferred← citeshttps://doi.org/10.1371/journal.pone.0280242openalex-snapshot
Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR
inferred← citeshttps://doi.org/10.1373/clinchem.2013.206375openalex-snapshot
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
inferred← citeshttps://doi.org/10.1373/clinchem.2014.221366openalex-snapshot
Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool
inferred← citeshttps://doi.org/10.1373/clinchem.2017.285478openalex-snapshot
Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine
inferred← citeshttps://doi.org/10.14456/tjg.2015.16openalex-snapshot
Development of highly sensitive detection for BCR-ABL transcripts in chronic myeloid leukemia by droplet digital PCR
inferred← citeshttps://doi.org/10.1515/almed-2022-0086openalex-snapshot
Diagnóstico genético prenatal de enfermedades monogénicas
inferred← citeshttps://doi.org/10.1515/almed-2023-0024openalex-snapshot
Prenatal genetic diagnosis of monogenic diseases
inferred← citeshttps://doi.org/10.1586/erm.11.50openalex-snapshot
Molecular diagnostics: harmonization through reference materials, documentary standards and proficiency testing
inferred← citeshttps://doi.org/10.2144/btn-2018-0058openalex-snapshot
Mitigation of the Effect of Variability in Digital PCR Assays through use of Duplexed Reference Assays for Normalization
inferred← citeshttps://doi.org/10.3109/10428194.2011.555569openalex-snapshot
Sensitive quantitation of minimal residual disease in chronic myeloid leukemia using nanofluidic digital polymerase chain reaction assay
inferred← citeshttps://doi.org/10.3389/fpls.2017.01670openalex-snapshot
Molecular Approaches for High Throughput Detection and Quantification of Genetically Modified Crops: A Review
inferred← citeshttps://doi.org/10.3390/cancers15092564openalex-snapshot
Methods to Evaluate Changes in Mitochondrial Structure and Function in Cancer
inferred← citeshttps://doi.org/10.3390/diseases9020035openalex-snapshot
Alignment of Qx100/Qx200 Droplet Digital (Bio-Rad) and QuantStudio 3D (Thermofisher) Digital PCR for Quantification of BCR-ABL1 in Ph+ Chronic Myeloid Leukemia
inferred← citeshttps://doi.org/10.3390/foods13050747openalex-snapshot
The Development of a Series of Genomic DNA Reference Materials with Specific Copy Number Ratios for The Detection of Genetically Modified Maize DBN9936
inferred← citeshttps://doi.org/10.3390/foods14010065openalex-snapshot
AcOTApks Gene-Based Molecular Tools to Improve Quantitative Detection of the Mycotoxigenic Fungus Aspergillus carbonarius
inferred← citeshttps://doi.org/10.3390/gidisord2030022openalex-snapshot
Circulating Tumour DNAs and Non-Coding RNAs as Liquid Biopsies for the Management of Colorectal Cancer Patients
inferred← citeshttps://doi.org/10.3390/pathogens10060693openalex-snapshot
The Usefulness of a Duplex RT-qPCR during the Recent Yellow Fever Brazilian Epidemic: Surveillance of Vaccine Adverse Events, Epizootics and Vectors
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