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Particle Size Reduction of Poly(ethylene terephthalate) Increases the Rate of Enzymatic Depolymerization But Does Not Increase the Overall Conversion Extent

https://doi.org/10.1021/acssuschemeng.2c01961
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures
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Ca 2+ and Mg 2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca
inferredcites →https://doi.org/10.1002/biot.201600008openalex-snapshot
A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films
inferredcites →https://doi.org/10.1002/biot.201600450openalex-snapshot
Polyester hydrolysis is enhanced by a truncated esterase: Less is more
inferredcites →https://doi.org/10.1002/biot.201700188openalex-snapshot
Engineered Humicola insolens cutinase for efficient cellulose acetate deacetylation
inferredcites →https://doi.org/10.1002/bit.24930openalex-snapshot
Surface engineering of a cutinase from Thermobifida cellulosilytica for improved polyester hydrolysis
inferredcites →https://doi.org/10.1002/bit.25941openalex-snapshot
Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition
inferredcites →https://doi.org/10.1002/bit.26372openalex-snapshot
Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilytica
inferredcites →https://doi.org/10.1002/btpr.610openalex-snapshot
Hydrolysis of polyethyleneterephthalate by p‐nitrobenzylesterase from Bacillus subtilis
inferredcites →https://doi.org/10.1002/cbic.202000793openalex-snapshot
Comparative Biochemistry of Four Polyester (PET) Hydrolases**
inferredcites →https://doi.org/10.1002/cssc.202101062openalex-snapshot
Low Carbon Footprint Recycling of Post‐Consumer PET Plastic with a Metagenomic Polyester Hydrolase
inferredcites →https://doi.org/10.1002/cssc.202101932openalex-snapshot
Comparative Performance of PETase as a Function of Reaction Conditions, Substrate Properties, and Product Accumulation
inferredcites →https://doi.org/10.1002/marc.200500410openalex-snapshot
Enzymatic Degradation of Poly(ethylene terephthalate): Rapid Hydrolyse using a Hydrolase from T. fusca
inferredcites →https://doi.org/10.1002/pen.11376openalex-snapshot
Evaluation of three methods for the measurement of crystallinity of pet resins, preforms, and bottles
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Toward rational thermostabilization of Aspergillus oryzae cutinase: Insights into catalytic and structural stability
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Structural and functional studies on a thermostable polyethylene terephthalate degrading hydrolase from Thermobifida fusca
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Surface engineering of polyester-degrading enzymes to improve efficiency and tune specificity
inferredcites →https://doi.org/10.1007/s10295-017-1942-zopenalex-snapshot
Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources
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Increase of the Hydrophilicity of Polyethylene Terephthalate Fibres by Hydrolases from Thermomonospora fusca and Fusarium solani f. sp. pisi
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Pet Waste Management by Chemical Recycling: A Review
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Characterization of strain-induced crystallization of poly(ethylene terephthalate) at fast draw rates using synchrotron radiation
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Synthetic Polyester-Hydrolyzing Enzymes From Thermophilic Actinomycetes
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A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes
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Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules
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Manufacturing energy and greenhouse gas emissions associated with plastics consumption
inferredcites →https://doi.org/10.1016/j.joule.2021.06.015openalex-snapshot
Techno-economic, life-cycle, and socioeconomic impact analysis of enzymatic recycling of poly(ethylene terephthalate)
inferredcites →https://doi.org/10.1016/j.mbs.2017.11.011openalex-snapshot
Michaelis–Menten equation for degradation of insoluble substrate
inferredcites →https://doi.org/10.1016/j.nbt.2015.11.004openalex-snapshot
Substrate specificities of cutinases on aliphatic–aromatic polyesters and on their model substrates
inferredcites →https://doi.org/10.1016/j.nbt.2022.02.006openalex-snapshot
Influence of substrate crystallinity and glass transition temperature on enzymatic degradation of polyethylene terephthalate (PET)
inferredcites →https://doi.org/10.1016/j.polymer.2020.122755openalex-snapshot
Understanding of strain-induced crystallization developments scenarios for polyesters: Comparison of poly(ethylene furanoate), PEF, and poly(ethylene terephthalate), PET
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Enzymatic recovery of polyester building blocks from polymer blends
inferredcites →https://doi.org/10.1016/j.procbio.2019.03.006openalex-snapshot
High-fold improvement of assorted post-consumer poly(ethylene terephthalate) (PET) packages hydrolysis using Humicola insolens cutinase as a single biocatalyst
inferredcites →https://doi.org/10.1016/j.wasman.2017.07.044openalex-snapshot
Mechanical and chemical recycling of solid plastic waste
inferredcites →https://doi.org/10.1016/j.ymben.2021.07.005openalex-snapshot
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440
inferredcites →https://doi.org/10.1016/s0032-3861(00)00128-2openalex-snapshot
Orientation prior to crystallisation during drawing of poly(ethylene terephthalate)
inferredcites →https://doi.org/10.1016/s0032-3861(99)00461-9openalex-snapshot
Observation of a transient structure prior to strain-induced crystallization in poly(ethylene terephthalate)
inferredcites →https://doi.org/10.1021/acs.biochem.7b01189openalex-snapshot
Stabilizing Leaf and Branch Compost Cutinase (LCC) with Glycosylation: Mechanism and Effect on PET Hydrolysis
inferredcites →https://doi.org/10.1021/acscatal.0c05126openalex-snapshot
Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy
inferredcites →https://doi.org/10.1021/acscatal.1c01204openalex-snapshot
Positive Charge Introduction on the Surface of Thermostabilized PET Hydrolase Facilitates PET Binding and Degradation
inferredcites →https://doi.org/10.1021/acscatal.1c05548openalex-snapshot
Conformational Selection in Biocatalytic Plastic Degradation by PETase
inferredcites →https://doi.org/10.1021/acscatal.1c05800openalex-snapshot
Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy
inferredcites →https://doi.org/10.1021/acscatal.1c05856openalex-snapshot
Mechanism-Based Design of Efficient PET Hydrolases
inferredcites →https://doi.org/10.1021/acscatal.7b00838openalex-snapshot
An Inverse Michaelis–Menten Approach for Interfacial Enzyme Kinetics
inferredcites →https://doi.org/10.1021/acscatal.9b00568openalex-snapshot
Rational Protein Engineering of Thermo-Stable PETase from Ideonella sakaiensis for Highly Efficient PET Degradation
inferredcites →https://doi.org/10.1021/acssuschemeng.0c01638openalex-snapshot
Current State and Perspectives Related to the Polyethylene Terephthalate Hydrolases Available for Biorecycling
inferredcites →https://doi.org/10.1021/bi401561popenalex-snapshot
Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase
inferredcites →https://doi.org/10.1021/bm400140uopenalex-snapshot
Fusion of Binding Domains to Thermobifida cellulosilytica Cutinase to Tune Sorption Characteristics and Enhancing PET Hydrolysis
inferredcites →https://doi.org/10.1021/ma9005318openalex-snapshot
Cutinase-Catalyzed Hydrolysis of Poly(ethylene terephthalate)
inferredcites →https://doi.org/10.1021/ma980137yopenalex-snapshot
Simple Kinetic Model for the Heterogeneous Enzymatic Hydrolysis of Natural Poly(3-hydroxybutyrate)
inferredcites →https://doi.org/10.1038/s41561-019-0335-5openalex-snapshot
Atmospheric transport and deposition of microplastics in a remote mountain catchment
inferredcites →https://doi.org/10.1038/s41586-020-2149-4openalex-snapshot
An engineered PET depolymerase to break down and recycle plastic bottles
inferredcites →https://doi.org/10.1038/s41586-022-04599-zopenalex-snapshot
Machine learning-aided engineering of hydrolases for PET depolymerization
inferredcites →https://doi.org/10.1038/s41598-019-52379-zopenalex-snapshot
Efficient Degradation of Poly(ethylene terephthalate) with Thermobifida fusca Cutinase Exhibiting Improved Catalytic Activity Generated using Mutagenesis and Additive-based Approaches
inferredcites →https://doi.org/10.1038/s41929-020-00521-wopenalex-snapshot
Possibilities and limitations of biotechnological plastic degradation and recycling
inferredcites →https://doi.org/10.1038/s41929-021-00648-4openalex-snapshot
Chemical and biological catalysis for plastics recycling and upcycling
inferredcites →https://doi.org/10.1038/s41929-022-00821-3openalex-snapshot
Directed evolution of an efficient and thermostable PET depolymerase
inferredcites →https://doi.org/10.1073/pnas.1314705111openalex-snapshot
Plastic debris in the open ocean
inferredcites →https://doi.org/10.1073/pnas.1718804115openalex-snapshot
Characterization and engineering of a plastic-degrading aromatic polyesterase
inferredcites →https://doi.org/10.1073/pnas.2006753117openalex-snapshot
Characterization and engineering of a two-enzyme system for plastics depolymerization
inferredcites →https://doi.org/10.1101/2021.10.10.463845openalex-snapshot
Deep learning redesign of PETase for practical PET degrading applications
inferredcites →https://doi.org/10.1111/1751-7915.12714openalex-snapshot
Biocatalysis as a green route for recycling the recalcitrant plastic polyethylene terephthalate
inferredcites →https://doi.org/10.1126/science.1260352openalex-snapshot
Plastic waste inputs from land into the ocean
inferredcites →https://doi.org/10.1126/science.aad6359openalex-snapshot
A bacterium that degrades and assimilates poly(ethylene terephthalate)
inferredcites →https://doi.org/10.1128/aem.04111-14openalex-snapshot
Enhanced Cutinase-Catalyzed Hydrolysis of Polyethylene Terephthalate by Covalent Fusion to Hydrophobins
inferredcites →https://doi.org/10.1128/aem.06725-11openalex-snapshot
Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Combined Adsorption and Michaelis–Menten Approach Reveals Predominant Enzymatic Depolymerization of Crystalline Poly(ethylene terephthalate) by Humicola insolens Cutinase in the Solution Phase
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Integrated Enzyme Engineering and One-Step Pretreatment Enable Scalable PET Waste Depolymerization
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Inactivation of PETase at Interfaces Inhibits PET Plastic Depolymerization
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Multilayer Packaging Films Designed for Deconstruction: Biopolyesters with Water-Soluble Barrier Layers
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Engineering and Application of a Thermostable MHETase for PET Depolymerization
inferred← citeshttps://doi.org/10.1021/acssusresmgt.6c00126openalex-snapshot
High-Capacity Enzymatic Degradation of Postconsumer Poly(ethylene terephthalate): Modeling and Experimental Investigations
inferred← citeshttps://doi.org/10.1021/acssynbio.5c00745openalex-snapshot
Computational Redesign of Thermo-Resistant MHETase for Complete Polyethylene Terephthalate Degradation by Dual-Enzyme System
inferred← citeshttps://doi.org/10.1021/jacs.3c08291openalex-snapshot
Ultrahigh-Throughput Directed Evolution of Polymer-Degrading Enzymes Using Yeast Display
inferred← citeshttps://doi.org/10.1038/s41467-022-35237-xopenalex-snapshot
Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity
inferred← citeshttps://doi.org/10.1038/s41467-025-60016-9openalex-snapshot
Standardization guidelines and future trends for PET hydrolase research
inferred← citeshttps://doi.org/10.1038/s41467-026-69970-4openalex-snapshot
Reflections on bio-based PET and plastic waste management: a responsible research and innovation approach
inferred← citeshttps://doi.org/10.1038/s41526-025-00463-2openalex-snapshot
Development and flight-testing of modular autonomous cultivation systems for biological plastics upcycling aboard the ISS
inferred← citeshttps://doi.org/10.1038/s41589-026-02210-4openalex-snapshot
Challenges and opportunities in the enzymatic recycling of nylons
inferred← citeshttps://doi.org/10.1038/s42003-023-05523-5openalex-snapshot
Computational design of highly efficient thermostable MHET hydrolases and dual enzyme system for PET recycling
inferred← citeshttps://doi.org/10.1038/s43246-025-00919-8openalex-snapshot
Recent advances in enzyme engineering for improved deconstruction of poly(ethylene terephthalate) (PET) plastics
inferred← citeshttps://doi.org/10.1038/s44222-025-00308-3openalex-snapshot
Polyester-degrading enzymes in a circular economy of plastics
inferred← citeshttps://doi.org/10.1038/s44286-024-00171-wopenalex-snapshot
Process insights for harnessing biotechnology for plastic depolymerization
inferred← citeshttps://doi.org/10.1038/s44286-025-00212-yopenalex-snapshot
Process innovations to enable viable enzymatic poly(ethylene terephthalate) recycling
inferred← citeshttps://doi.org/10.1039/d2gc01965eopenalex-snapshot
Conversion of polyethylene terephthalate into pure terephthalic acid through synergy between a solid-degrading cutinase and a reaction intermediate-hydrolysing carboxylesterase
inferred← citeshttps://doi.org/10.1039/d4fd00014eopenalex-snapshot
Degradation of PET microplastic particles to monomers in human serum by PETase
inferred← citeshttps://doi.org/10.1039/d4gc00659copenalex-snapshot
Characterization of polymer properties and identification of additives in commercially available research plastics
inferred← citeshttps://doi.org/10.1039/d4su00722kopenalex-snapshot
Analysis of the product-spectrum during the biocatalytic hydrolysis of PEF (poly(ethylene furanoate)) with various esterases
inferred← citeshttps://doi.org/10.1039/d5fd00061kopenalex-snapshot
Is solvent-based dissolution and precipitation an effective substrate pretreatment for the enzymatic depolymerisation of poly(ethylene terephthalate)?
inferred← citeshttps://doi.org/10.1039/d5gc00248fopenalex-snapshot
Industrially viable and selective catalytic system: simple and sustainable pathway for efficient degradation of waste polyester textiles
inferred← citeshttps://doi.org/10.1039/d5gc02621kopenalex-snapshot
Plastic photoreforming: catalytic production of hydrogen and valuable chemicals
inferred← citeshttps://doi.org/10.1039/d5py00023hopenalex-snapshot
Factors modulating the hydrolysis of Nylon-6,6 by a nylon hydrolase enzyme
inferred← citeshttps://doi.org/10.1101/2025.11.26.690883openalex-snapshot
Process optimization for enhanced enzymatic nylon deconstruction
inferred← citeshttps://doi.org/10.1111/febs.70734openalex-snapshot
Crystal structure and biochemical analysis of the polyester‐degrading carboxylesterase SM0281 from the symbiotic legume‐associated rhizobacterium Sinorhizobium (Ensifer) meliloti
inferred← citeshttps://doi.org/10.1186/s12934-024-02547-2openalex-snapshot
Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes
inferred← citeshttps://doi.org/10.1186/s13568-022-01474-yopenalex-snapshot
Efficient depolymerization of polyethylene terephthalate (PET) and polyethylene furanoate by engineered PET hydrolase Cut190
inferred← citeshttps://doi.org/10.1360/ssc-2025-0272openalex-snapshot
Research progress on polyester chemical recycling and industrialization
inferred← citeshttps://doi.org/10.2139/ssrn.7193082openalex-snapshot
Advanced Process Engineering Strategies for the Enzymatic Degradation of Polyethylene Terephthalate (PET) in Continuous Stirred Tank Reactor
inferred← citeshttps://doi.org/10.32604/jrm.2023.025032openalex-snapshot
Recycling Carbon Resources from Waste PET to Reduce Carbon Dioxide Emission: Carbonization Technology Review and Perspective
inferred← citeshttps://doi.org/10.3390/catal13030591openalex-snapshot
Determinants for an Efficient Enzymatic Catalysis in Poly(Ethylene Terephthalate) Degradation
inferred← citeshttps://doi.org/10.3390/catal16070580openalex-snapshot
Enzymatic Degradation of Crystalline Polyethylene Terephthalate: Challenges, Strategies, and Perspectives Towards Sustainable Recycling
inferred← citeshttps://doi.org/10.3390/ijms24043877openalex-snapshot
Microbial Enzyme Biotechnology to Reach Plastic Waste Circularity: Current Status, Problems and Perspectives
inferred← citeshttps://doi.org/10.3390/polym17060763openalex-snapshot
Efficient Bioprocess for Mixed PET Waste Depolymerization Using Crude Cutinase
inferred← citeshttps://doi.org/10.3390/resources14110176openalex-snapshot
Biocatalytic Recycling of Polyethylene Terephthalate: From Conventional to Innovative Routes for Transforming Plastic and Textile Waste into Renewable Resources
inferred← citeshttps://doi.org/10.3390/su142315898openalex-snapshot
A Review of Cross-Disciplinary Approaches for the Identification of Novel Industrially Relevant Plastic-Degrading Enzymes
inferred← citeshttps://doi.org/10.4014/jmb.2604.04057openalex-snapshot
Microbial and Enzymatic Degradation of Nylon: Mechanisms, Diversity, and Biotechnological Application
inferred← citeshttps://doi.org/10.57634/rcr5069openalex-snapshot
Catalytic degradation of microplastics
inferred← citeshttps://doi.org/10.6084/m9.figshare.21405871openalex-snapshot
Additional file 1 of Efficient depolymerization of polyethylene terephthalate (PET) and polyethylene furanoate by engineered PET hydrolase Cut190
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