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Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes
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What this work says
Outgoing claims: the papers this work cites, builds on, or argues with.
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An improved protocol for the preparation of yeast cells for transformation by electroporation
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New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiae
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Tn5: A Molecular Window on Transposition
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Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn 5 transposase 1 1Edited by G. Smith
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Molecular cloning: A laboratory manual
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Nucleotide sequence of the kanamycin resistance transposon Tn903
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Construction and characterization of new cloning vehicle. II. A multipurpose cloning system
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A procedure for the isolation of deoxyribonucleic acid from micro-organisms
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Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
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Systematic identification of essential genes by in vitro mariner mutagenesis
inferredcites →https://doi.org/10.1093/nar/13.20.7207openalex-snapshot
Rapid transfer of DNA from agarose gels to nylon membranes
inferredcites →https://doi.org/10.1093/nar/16.13.6127openalex-snapshot
High efficiency transformation of E.coli by high voltage electroporation
inferredcites →https://doi.org/10.1093/nar/20.4.811openalex-snapshot
Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K
inferredcites →https://doi.org/10.1093/nar/22.18.3765openalex-snapshot
Efficient integration of artificial transposons into plasmid targetsin vitro: a useful tool for DNA mapping, sequencing and genetic analysis
inferredcites →https://doi.org/10.1093/nar/27.13.2777openalex-snapshot
An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications
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Sequencing Multimegabase-Template DNA with BigDye Terminator Chemistry
inferredcites →https://doi.org/10.1108/rr.2001.15.2.36.96openalex-snapshot
Embryonic Encyclopedia of Life Sciences
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The Complete Genome Sequence of Escherichia coli K-12
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Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
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In vitro Tn7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation
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High-density transposon libraries utilising outward-oriented promoters identify mechanisms of action and resistance to antimicrobials
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Instability of CII is needed for efficient switching between lytic and lysogenic development in bacteriophage 186
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Efficient cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into transcriptionally active mitochondria
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The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi
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Eliminating helper phage from phage display
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An ultra-dense library resource for rapid deconvolution of mutations that cause phenotypes inEscherichia coli
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Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection
inferred← citeshttps://doi.org/10.1093/nar/gnf118openalex-snapshot
Production of combinatorial libraries of fused genes by sequential transposition reactions
inferred← citeshttps://doi.org/10.1093/nar/gni154openalex-snapshot
Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes
inferred← citeshttps://doi.org/10.1094/mpmi.2001.14.6.701openalex-snapshot
Transposon Mutagenesis of Xylella fastidiosa by Electroporation of Tn5 Synaptic Complexes
inferred← citeshttps://doi.org/10.1095/biolreprod.105.044669openalex-snapshot
Tn5 Transposase-Mediated Mouse Transgenesis1
inferred← citeshttps://doi.org/10.1099/00221287-147-9-2529openalex-snapshot
Construction of Rhodococcus random mutagenesis libraries using Tn5 transposition complexes
inferred← citeshttps://doi.org/10.1099/acmi.0.000107openalex-snapshot
Toward a genetic system in the marine cyanobacterium Prochlorococcus
inferred← citeshttps://doi.org/10.1099/jmm.0.000081openalex-snapshot
Genetic tools for manipulating Acinetobacter baumannii genome: an overview
inferred← citeshttps://doi.org/10.1099/mic.0.061705-0openalex-snapshot
An update on the molecular genetics toolbox for staphylococci
inferred← citeshttps://doi.org/10.1099/mic.0.27807-0openalex-snapshot
Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes
inferred← citeshttps://doi.org/10.1099/mic.0.29121-0openalex-snapshot
Genetic tools for highly pathogenic Francisella tularensis subsp. tularensis
inferred← citeshttps://doi.org/10.1101/2024.06.04.597434openalex-snapshot
A high-throughput search for intracellular factors that affect RNA folding identifies E. coli proteins PepA and YagL as RNA chaperones that promote RNA remodeling
inferred← citeshttps://doi.org/10.1101/2024.07.17.604004openalex-snapshot
Identifying essential genes in Schaalia odontolytica using a highly-saturated transposon library
inferred← citeshttps://doi.org/10.1101/gr.1710304openalex-snapshot
Systematic Insertional Mutagenesis of a Streptomycete Genome: A Link Between Osmoadaptation and Antibiotic Production
inferred← citeshttps://doi.org/10.1101/gr.611403openalex-snapshot
Chromosomal Deletion Formation System Based on Tn5 Double Transposition: Use For Making Minimal Genomes and Essential Gene Analysis
inferred← citeshttps://doi.org/10.1111/ajgw.12090openalex-snapshot
Manganese transporter protein MntH is required for virulence ofXylophilus ampelinus, the causal agent of bacterial necrosis in grapevine
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2010.04690.xopenalex-snapshot
A practical random mutagenesis system for probiotic Lactobacillus casei using Tn 5 transposition complexes
inferred← citeshttps://doi.org/10.1111/j.1365-2958.2005.04794.xopenalex-snapshot
Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress
inferred← citeshttps://doi.org/10.1111/j.1365-3059.2011.02519.xopenalex-snapshot
Tn5 transposon mutagenesis in Acidovorax citrulli for identification of genes required for pathogenicity on cucumber
inferred← citeshttps://doi.org/10.1111/j.1365-313x.2011.04663.xopenalex-snapshot
Tn5 transposase‐assisted transformation of indica rice
inferred← citeshttps://doi.org/10.1111/j.1574-6968.2008.01430.xopenalex-snapshot
Development and use of a selectable, broad-host-range reporter transposon for identifying environmentally regulated promoters in bacteria
inferred← citeshttps://doi.org/10.1111/j.1574-6968.2009.01858.xopenalex-snapshot
The Afipia toolbox and its use to isolate flagellar mutants
inferred← citeshttps://doi.org/10.1111/j.1751-7915.2007.00001.xopenalex-snapshot
The application of Tet repressor in prokaryotic gene regulation and expression
inferred← citeshttps://doi.org/10.1111/mmi.12541openalex-snapshot
Activation of phenotypic subpopulations in response to ciprofloxacin treatment in A cinetobacter baumannii
inferred← citeshttps://doi.org/10.1126/science.aao0679openalex-snapshot
Structures of the CRISPR genome integration complex
inferred← citeshttps://doi.org/10.1128/9781555816506.ch19openalex-snapshot
Recombineering in Prokaryotes
inferred← citeshttps://doi.org/10.1128/9781555816827.ch19openalex-snapshot
Strain Improvement of Escherichia coli To Enhance Recombinant Protein Production
inferred← citeshttps://doi.org/10.1128/9781555817497.ch16openalex-snapshot
General Methods To Investigate Microbial Symbioses
inferred← citeshttps://doi.org/10.1128/9781555817954.ch3openalex-snapshot
Putting Mobile DNA to Work: the Toolbox
inferred← citeshttps://doi.org/10.1128/aac.47.4.1267-1274.2003openalex-snapshot
Isolation of Antibiotic Hypersusceptibility Mutants of Acinetobacter spp. by Selection for DNA Release
inferred← citeshttps://doi.org/10.1128/aem.00411-07openalex-snapshot
Efficient Method of Cloning the Obligate Intracellular Bacterium Coxiella burnetii
inferred← citeshttps://doi.org/10.1128/aem.00492-06openalex-snapshot
Generating Tetracycline-Inducible Auxotrophy in Escherichia coli and Salmonella enterica Serovar Typhimurium by Using an Insertion Element and a Hyperactive Transposase
inferred← citeshttps://doi.org/10.1128/aem.00531-12openalex-snapshot
Development of an Efficient In Vivo System (P junc -TpaseIS 1223 ) for Random Transposon Mutagenesis of Lactobacillus casei
inferred← citeshttps://doi.org/10.1128/aem.00739-06openalex-snapshot
Random Insertion of a TetR-Inducing Peptide Tag into Escherichia coli Proteins Allows Analysis of Protein Levels by Induction of Reporter Gene Expression
inferred← citeshttps://doi.org/10.1128/aem.02882-07openalex-snapshot
Identification of Differentially Regulated Francisella tularensis Genes by Use of a Newly Developed Tn 5 -Based Transposon Delivery System
inferred← citeshttps://doi.org/10.1128/aem.03374-16openalex-snapshot
Development of oriC -Based Plasmids for Mesoplasma florum
inferred← citeshttps://doi.org/10.1128/aem.03408-14openalex-snapshot
Genome-Wide Identification of Genes Necessary for Biofilm Formation by Nosocomial Pathogen Stenotrophomonas maltophilia Reveals that Orphan Response Regulator FsnR Is a Critical Modulator
inferred← citeshttps://doi.org/10.1128/aem.68.2.705-712.2002openalex-snapshot
Efficient Insertion Mutagenesis Strategy for Bacterial Genomes Involving Electroporation of In Vitro-Assembled DNA Transposition Complexes of Bacteriophage Mu
inferred← citeshttps://doi.org/10.1128/aem.70.11.6901-6904.2004openalex-snapshot
Use of Transposon-Transposase Complexes To Create Stable Insertion Mutant Strains of Francisella tularensis LVS
inferred← citeshttps://doi.org/10.1128/aem.70.5.2816-2822.2004openalex-snapshot
Transposon Mutagenesis of the Obligate Intracellular Pathogen Rickettsia prowazekii
inferred← citeshttps://doi.org/10.1128/aem.71.1.451-459.2005openalex-snapshot
Phenotypic Screening of Escherichia coli K-12 Tn 5 Insertion Libraries, Using Whole-Genome Oligonucleotide Microarrays
inferred← citeshttps://doi.org/10.1128/aem.72.5.3418-3428.2006openalex-snapshot
Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum
inferred← citeshttps://doi.org/10.1128/aem.72.5.3750-3755.2006openalex-snapshot
High-Throughput Transposon Mutagenesis of Corynebacterium glutamicum and Construction of a Single-Gene Disruptant Mutant Library
inferred← citeshttps://doi.org/10.1128/iai.00228-23openalex-snapshot
Transposon sequencing identifies genes impacting Staphylococcus aureus invasion in a human macrophage model
inferred← citeshttps://doi.org/10.1128/iai.00598-06openalex-snapshot
AttenuatedFrancisella novicidaTransposon Mutants Protect Mice against Wild-Type Challenge
inferred← citeshttps://doi.org/10.1128/iai.01036-08openalex-snapshot
Interconnections between Sigma B, agr , and Proteolytic Activity in Staphylococcus aureus Biofilm Maturation
inferred← citeshttps://doi.org/10.1128/iai.01721-07openalex-snapshot
tRNA Modification by GidA/MnmE Is Necessary forStreptococcus pyogenesVirulence: a New Strategy To Make Live Attenuated Strains
inferred← citeshttps://doi.org/10.1128/iai.73.10.6260-6271.2005openalex-snapshot
Identification of New Secreted Effectors in Salmonella enterica Serovar Typhimurium
inferred← citeshttps://doi.org/10.1128/jb.00060-17openalex-snapshot
The Histidine Residue of QseC Is Required for Canonical Signaling between QseB and PmrB in Uropathogenic Escherichia coli
inferred← citeshttps://doi.org/10.1128/jb.00164-25openalex-snapshot
Identifying essential genes in Schaalia odontolytica using a saturated transposon library
inferred← citeshttps://doi.org/10.1128/jb.00205-06openalex-snapshot
Albomycin Uptake via a Ferric Hydroxamate Transport System ofStreptococcus pneumoniaeR6
inferred← citeshttps://doi.org/10.1128/jb.00215-18openalex-snapshot
Pseudomonas aeruginosa Regulated Intramembrane Proteolysis: Protease MucP Can Overcome Mutations in the AlgO Periplasmic Protease To Restore Alginate Production in Nonmucoid Revertants
inferred← citeshttps://doi.org/10.1128/jb.00352-17openalex-snapshot
Comprehensive Arrayed Transposon Mutant Library of Klebsiella pneumoniae Outbreak Strain KPNIH1
inferred← citeshttps://doi.org/10.1128/jb.00621-10openalex-snapshot
Silent Mischief: Bacteriophage Mu Insertions Contaminate Products of Escherichia coli Random Mutagenesis Performed Using Suicidal Transposon Delivery Plasmids Mobilized by Broad-Host-Range RP4 Conjugative Machinery
inferred← citeshttps://doi.org/10.1128/jb.01012-12openalex-snapshot
Transposon Mutagenesis Identifies Uropathogenic Escherichia coli Biofilm Factors
inferred← citeshttps://doi.org/10.1128/jb.01170-08openalex-snapshot
Growth Deficiencies ofNeisseria meningitidis pfsandluxSMutants Are Not Due to Inactivation of Quorum Sensing
inferred← citeshttps://doi.org/10.1128/jb.01589-14openalex-snapshot
Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation
inferred← citeshttps://doi.org/10.1128/jb.184.16.4555-4572.2002openalex-snapshot
From Genetic Footprinting to Antimicrobial Drug Targets: Examples in Cofactor Biosynthetic Pathways
inferred← citeshttps://doi.org/10.1128/jb.184.20.5723-5732.2002openalex-snapshot
Construction and Characterization of Transposon Insertion Mutations inCorynebacterium diphtheriaeThat Affect Expression of the Diphtheria Toxin Repressor (DtxR)
inferred← citeshttps://doi.org/10.1128/jb.185.16.5003-5006.2003openalex-snapshot
Mutational Analysis of Cell Wall Biosynthesis inMycobacterium avium
inferred← citeshttps://doi.org/10.1128/jb.185.19.5673-5684.2003openalex-snapshot
Experimental Determination and System Level Analysis of Essential Genes in Escherichia coli MG1655
inferred← citeshttps://doi.org/10.1128/jb.186.15.4921-4930.2004openalex-snapshot
Systematic Mutagenesis of the Escherichia coli Genome
inferred← citeshttps://doi.org/10.1128/jb.187.10.3438-3444.2005openalex-snapshot
The Iron-Regulated iupABC Operon Is Required for Saprophytic Growth of the Intracellular Pathogen Rhodococcus equi at Low Iron Concentrations
inferred← citeshttps://doi.org/10.1128/jb.187.13.4598-4606.2005openalex-snapshot
Genetic Evidence that GTP Is Required for Transposition of IS 903 and Tn 552 in Escherichia coli
inferred← citeshttps://doi.org/10.1128/jvi.01766-08openalex-snapshot
Temporal Control of Message Stability in the Life Cycle of Double-Stranded RNA Bacteriophage Φ8
inferred← citeshttps://doi.org/10.1128/jvi.77.1.123-134.2003openalex-snapshot
A Direct Transposon Insertion Tool for Modification and Functional Analysis of Viral Genomes
inferred← citeshttps://doi.org/10.1128/mbio.00306-15openalex-snapshot
Rapid Quantification of Mutant Fitness in Diverse Bacteria by Sequencing Randomly Bar-Coded Transposons
inferred← citeshttps://doi.org/10.1128/mbio.02118-15openalex-snapshot
Investigation of the Genetics and Biochemistry of Roseobacticide Production in the Roseobacter Clade Bacterium Phaeobacter inhibens
inferred← citeshttps://doi.org/10.1128/spectrum.04306-23openalex-snapshot
PstSCAB and SapBCDF are putrescine exporters in Proteus mirabilis
inferred← citeshttps://doi.org/10.1139/w11-026openalex-snapshot
Selection and characterization ofSpiroplasma citrimutants by random transposome mutagenesis
inferred← citeshttps://doi.org/10.1146/annurev.ento.54.110807.090545openalex-snapshot
Insect Transgenesis: Current Applications and Future Prospects
inferred← citeshttps://doi.org/10.1146/annurev.genet.36.061102.093104openalex-snapshot
Genetic Engineering Using Homologous Recombination
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