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Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Microsolidics: Fabrication of Three‐Dimensional Metallic Microstructures in Poly(dimethylsiloxane)
inferredcites →https://doi.org/10.1002/anie.200390203openalex-snapshot
A Microfluidic System for Controlling Reaction Networks in Time
inferredcites →https://doi.org/10.1002/anie.200503540openalex-snapshot
Electric Control of Droplets in Microfluidic Devices
inferredcites →https://doi.org/10.1002/anie.200704903openalex-snapshot
From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices
inferredcites →https://doi.org/10.1002/cbic.200700536openalex-snapshot
An Integrated Device for Monitoring Time‐Dependent in vitro Expression From Single Genes in Picolitre Droplets
inferredcites →https://doi.org/10.1002/cyto.a.20390openalex-snapshot
International Society for Analytical Cytology Biosafety Standard for Sorting of Unfixed Cells
inferredcites →https://doi.org/10.1016/j.chembiol.2005.09.012openalex-snapshot
High-Throughput Screening of Enzyme Libraries: Thiolactonases Evolved by Fluorescence-Activated Sorting of Single Cells in Emulsion Compartments
inferredcites →https://doi.org/10.1016/j.chembiol.2005.09.016openalex-snapshot
High-Throughput Screening of Enzyme Libraries: In Vitro Evolution of a β-Galactosidase by Fluorescence-Activated Sorting of Double Emulsions
inferredcites →https://doi.org/10.1016/j.chembiol.2008.04.004openalex-snapshot
Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms
inferredcites →https://doi.org/10.1016/j.tibtech.2006.06.009openalex-snapshot
Miniaturising the laboratory in emulsion droplets
inferredcites →https://doi.org/10.1016/s0022-1759(00)00219-2openalex-snapshot
Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC)
inferredcites →https://doi.org/10.1021/ac8007779openalex-snapshot
Microfluidic-Based Cell Sorting of Francisella tularensis Infected Macrophages Using Optical Forces
inferredcites →https://doi.org/10.1021/ac980656zopenalex-snapshot
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane)
inferredcites →https://doi.org/10.1038/4411179aopenalex-snapshot
Divide and conquer
inferredcites →https://doi.org/10.1038/nbt0798-652openalex-snapshot
Man-made cell-like compartments for molecular evolution
inferredcites →https://doi.org/10.1038/nmeth0205-91aopenalex-snapshot
FACS-on-a-chip
inferredcites →https://doi.org/10.1039/b618570copenalex-snapshot
Quantitative detection of protein expression in single cells using droplet microfluidics
inferredcites →https://doi.org/10.1039/b802941eopenalex-snapshot
Drop-based microfluidic devices for encapsulation of single cells
inferredcites →https://doi.org/10.1039/b805456hopenalex-snapshot
Controlled encapsulation of single-cells into monodisperse picolitre drops
inferredcites →https://doi.org/10.1039/b806706fopenalex-snapshot
Biocompatible surfactants for water-in-fluorocarbon emulsions
inferredcites →https://doi.org/10.1063/1.1537519openalex-snapshot
Formation of dispersions using “flow focusing” in microchannels
inferredcites →https://doi.org/10.1063/1.2164911openalex-snapshot
Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices
inferredcites →https://doi.org/10.1063/1.2218058openalex-snapshot
Electrocoalescence of drops synchronized by size-dependent flow in microfluidic channels
inferredcites →https://doi.org/10.1063/1.2357039openalex-snapshot
Controlled electrocoalescence in microfluidics: Targeting a single lamella
inferredcites →https://doi.org/10.1073/pnas.0708321105openalex-snapshot
Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells
inferredcites →https://doi.org/10.1103/physreve.74.035303openalex-snapshot
Droplet breakup in microfluidic junctions of arbitrary angles
inferredcites →https://doi.org/10.1103/physreve.75.046302openalex-snapshot
Thermocapillary valve for droplet production and sorting
inferredcites →https://doi.org/10.1103/physrevlett.92.054503openalex-snapshot
Geometrically Mediated Breakup of Drops in Microfluidic Devices
inferredcites →https://doi.org/10.1128/aem.56.12.3861-3866.1990openalex-snapshot
Flow cytometry sorting of viable bacteria and yeasts according to beta-galactosidase activity
inferredcites →https://doi.org/10.1146/annurev.bi.46.070177.003451openalex-snapshot
Polymyxin and Related Peptide Antibiotics
inferredcites →https://doi.org/10.1177/1087057108319644openalex-snapshot
The Future of High-Throughput Screening

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology
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Mass Activated Droplet Sorting (MADS) Enables High‐Throughput Screening of Enzymatic Reactions at Nanoliter Scale
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Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey
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Activity‐Fed Translation (AFT) Assay: A New High‐Throughput Screening Strategy for Enzymes in Droplets
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Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay
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Rapid, Label‐Free Screening of Diverse Biotransformations by Flow‐Injection Mass Spectrometry
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Microfluidic sorting of fluorescently activated cells depending on gene expression level
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Image‐based sorting and negative dielectrophoresis for high purity cell and particle separation
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A Low‐Voltage–Driven Droplet Sorter for High‐Stability and Small‐Deformation Droplet Sorting
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Growing microbes in millifluidic droplets
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Advances in automated real‐time flow cytometry for monitoring of bioreactor processes
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Progress of Cell Sorting in Flow Cytometry
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Miniaturized Droplet‐Based Adaptation of the Ames Test for High‐Throughput Mutagenicity Assessment
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Microfluidics Based Particle and Droplet Generation for Gene and Drug Delivery Approaches
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Biochemie 2009
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Dynamic Interfacial Printing Driven by Galvanometer Scanner for Monodisperse Single and Double Emulsion Droplets
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Microfluidic Automation Using Elastomeric Valves and Droplets: Reducing Reliance on External Controllers
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Monodisperse Emulsion Drop Microenvironments for Bacterial Biofilm Growth
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Standing Surface Acoustic Wave (SSAW)‐Based Fluorescence‐Activated Cell Sorter
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A Gelatin Microdroplet Platform for High‐Throughput Sorting of Hyperproducing Single‐Cell‐Derived Microalgal Clones
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Double Emulsion Flow Cytometry for Rapid Single Genome Detection
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Single-Cell Manipulation
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Enzyme Evolution
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Biotechnological Importance of Actinomycetes
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Single-molecule emulsion PCR in microfluidic droplets
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High-throughput, multiparameter analysis of single cells
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Dielectrophoretic effect on droplet dynamic behaviors in microchannels
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Biosensor-enabled droplet microfluidic system for the rapid screening of 3-dehydroshikimic acid produced in Escherichia coli
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Size-dependent microparticles separation through standing surface acoustic waves
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Traffic of pairs of drops in microfluidic ladder networks with fore-aft structural asymmetry
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Selective handling of droplets in a microfluidic device using magnetic rails
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Soft lithography fabrication of index-matched microfluidic devices for reducing artifacts in fluorescence and quantitative phase imaging
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Passive sorting of emulsion droplets with different interfacial properties using laser-patterned surfaces
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Particle timing and spacing control in microchannel flow by applying periodic force over space and time
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Controllable droplet generation at a microfluidic T-junction using AC electric field
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The role of channel height and actuation method on particle manipulation in surface acoustic wave (SAW)-driven microfluidic devices
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Multiple interactions between microfluidic droplets and on-chip pneumatic valves
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Advanced microfluidic droplet manipulation based on piezoelectric actuation
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Encapsulation of single cells on a microfluidic device integrating droplet generation with fluorescence-activated droplet sorting
inferred← citeshttps://doi.org/10.1007/s10544-018-0310-8openalex-snapshot
Prediction of Microdroplet Breakup Regime in Asymmetric T-Junction Microchannels
inferred← citeshttps://doi.org/10.1007/s10544-019-0401-1openalex-snapshot
Multi-purpose machine vision platform for different microfluidics applications
inferred← citeshttps://doi.org/10.1007/s11047-018-9715-9openalex-snapshot
Scaling up genetic circuit design for cellular computing: advances and prospects
inferred← citeshttps://doi.org/10.1007/s11265-018-1372-9openalex-snapshot
Embedded Fluorescence Lifetime Determination for High-Throughput, Low-Photon-Number Applications
inferred← citeshttps://doi.org/10.1007/s11517-010-0611-4openalex-snapshot
Microfluidics for cell separation
inferred← citeshttps://doi.org/10.1007/s11705-014-1412-3openalex-snapshot
Materials and surface engineering to control bacterial adhesion and biofilm formation: A review of recent advances
inferred← citeshttps://doi.org/10.1007/s12668-012-0051-8openalex-snapshot
Applications of Microfluidics in Stem Cell Biology
inferred← citeshttps://doi.org/10.1007/s13206-014-9101-3openalex-snapshot
Continuous oxygen supply in pump-less micro-bioreactor based on microfluidics
inferred← citeshttps://doi.org/10.1007/s13206-022-00067-yopenalex-snapshot
Imaging Technologies for Microfluidic Biochips
inferred← citeshttps://doi.org/10.1007/s42995-020-00082-8openalex-snapshot
One cell at a time: droplet-based microbial cultivation, screening and sequencing
inferred← citeshttps://doi.org/10.1016/b978-0-08-055294-1.00114-8openalex-snapshot
Materials to Control and Measure Cell Function
inferred← citeshttps://doi.org/10.1016/b978-0-08-100691-7.00246-9openalex-snapshot
3.17 Materials to Control and Measure Cell Function
inferred← citeshttps://doi.org/10.1016/b978-0-08-102302-0.00006-7openalex-snapshot
Colloidal Microfluidics
inferred← citeshttps://doi.org/10.1016/b978-0-12-374912-3.00002-xopenalex-snapshot
Droplet Microfluidics for High-throughput Analysis of Cells and Particles
inferred← citeshttps://doi.org/10.1016/b978-0-12-374912-3.00005-5openalex-snapshot
Rise of the Micromachines: Microfluidics and the Future of Cytometry
inferred← citeshttps://doi.org/10.1016/b978-0-12-812659-2.00001-6openalex-snapshot
Lab-on-a-chip technology and microfluidics
inferred← citeshttps://doi.org/10.1016/b978-0-12-812659-2.00013-2openalex-snapshot
Single-cell screening using microfluidic systems
inferred← citeshttps://doi.org/10.1016/b978-0-12-819583-3.00010-2openalex-snapshot
Numerical modeling of cell separation in microfluidic chips
inferred← citeshttps://doi.org/10.1016/b978-0-12-819820-9.00005-3openalex-snapshot
Enzyme engineering strategies to confer thermostability
inferred← citeshttps://doi.org/10.1016/b978-0-12-819820-9.00007-7openalex-snapshot
Protein engineering approaches for enhanced catalytic efficiency
inferred← citeshttps://doi.org/10.1016/b978-0-12-819971-8.00013-5openalex-snapshot
Microfluidic devices for stem cell analysis
inferred← citeshttps://doi.org/10.1016/b978-0-12-820472-6.00078-5openalex-snapshot
Compound Screening
inferred← citeshttps://doi.org/10.1016/b978-0-12-821753-5.00007-1openalex-snapshot
High-throughput screening for improving cellular and enzymatic properties
inferred← citeshttps://doi.org/10.1016/b978-0-12-822945-3.00023-3openalex-snapshot
Structure-function relationship of extremozymes
inferred← citeshttps://doi.org/10.1016/b978-0-12-823536-2.00002-xopenalex-snapshot
Cell-based assays on microfluidic chips
inferred← citeshttps://doi.org/10.1016/b978-0-323-95213-2.00017-xopenalex-snapshot
Separation and characterization of cells using electrical field
inferred← citeshttps://doi.org/10.1016/b978-0-323-95478-5.00027-3openalex-snapshot
Droplet-Based Assays
inferred← citeshttps://doi.org/10.1016/b978-0-443-15416-4.00002-5openalex-snapshot
Droplet-based microfluidics for biological applications
inferred← citeshttps://doi.org/10.1016/b978-0-443-15416-4.00004-9openalex-snapshot
Challenges and opportunities
inferred← citeshttps://doi.org/10.1016/b978-0-443-15416-4.00006-2openalex-snapshot
Droplet and digital microfluidics–enabled analytical techniques
inferred← citeshttps://doi.org/10.1016/b978-0-444-53349-4.00180-1openalex-snapshot
Soft Lithographic Approaches to Nanofabrication
inferred← citeshttps://doi.org/10.1016/bs.acc.2016.10.001openalex-snapshot
Droplet-Based Digital PCR
inferred← citeshttps://doi.org/10.1016/bs.mcb.2018.09.006openalex-snapshot
Quantifying phenotypes in single cells using droplet microfluidics
inferred← citeshttps://doi.org/10.1016/bs.mie.2019.07.009openalex-snapshot
Single-cell activity screening in microfluidic droplets
inferred← citeshttps://doi.org/10.1016/bs.mie.2020.05.004openalex-snapshot
Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterization
inferred← citeshttps://doi.org/10.1016/bs.mie.2020.06.002openalex-snapshot
Ultrahigh throughput screening for enzyme function in droplets
inferred← citeshttps://doi.org/10.1016/bs.pmbts.2026.08.018openalex-snapshot
Microfluidic devices for lab-on-chip growth
inferred← citeshttps://doi.org/10.1016/j.ab.2013.09.028openalex-snapshot
An Amplex Red-based fluorometric and spectrophotometric assay for l-asparaginase using its natural substrate
inferred← citeshttps://doi.org/10.1016/j.aca.2013.04.064openalex-snapshot
Analytical detection techniques for droplet microfluidics—A review
inferred← citeshttps://doi.org/10.1016/j.aca.2017.02.024openalex-snapshot
Review: Microbial analysis in dielectrophoretic microfluidic systems
inferred← citeshttps://doi.org/10.1016/j.aca.2017.11.063openalex-snapshot
Fabricating smooth PDMS microfluidic channels from low-resolution 3D printed molds using an omniphobic lubricant-infused coating
inferred← citeshttps://doi.org/10.1016/j.aca.2019.08.025openalex-snapshot
Sorting by interfacial tension (SIFT): Label-free enzyme sorting using droplet microfluidics
inferred← citeshttps://doi.org/10.1016/j.aca.2021.338212openalex-snapshot
Controlling water transport between micelles and aqueous microdroplets during sample enrichment
inferred← citeshttps://doi.org/10.1016/j.aca.2021.339344openalex-snapshot
A 3D printed size-tunable flow-focusing droplet microdevice to produce cell-laden hydrogel microspheres
inferred← citeshttps://doi.org/10.1016/j.aca.2022.340342openalex-snapshot
A chip-based supersonic microfluidic nebulizer for efficient sample introduction into inductively coupled plasma – Mass spectrometry
inferred← citeshttps://doi.org/10.1016/j.aca.2024.343043openalex-snapshot
Detachable acoustofluidic droplet-sorter
inferred← citeshttps://doi.org/10.1016/j.actbio.2020.05.004openalex-snapshot
Past, Present, and Future of Affinity-based Cell Separation Technologies
inferred← citeshttps://doi.org/10.1016/j.addr.2013.02.001openalex-snapshot
On-chip investigation of cell–drug interactions
inferred← citeshttps://doi.org/10.1016/j.addr.2022.114143openalex-snapshot
Tools for computational design and high-throughput screening of therapeutic enzymes
inferred← citeshttps://doi.org/10.1016/j.apsb.2024.05.003openalex-snapshot
Screening antimicrobial peptides and probiotics using multiple deep learning and directed evolution strategies
inferred← citeshttps://doi.org/10.1016/j.bbagen.2010.04.009openalex-snapshot
Miniaturization of biological assays — Overview on microwell devices for single-cell analyses
inferred← citeshttps://doi.org/10.1016/j.bej.2022.108586openalex-snapshot
Combining genetically encoded biosensors with droplet microfluidic system for enhanced glutaminase production by Bacillus amyloliquefaciens
inferred← citeshttps://doi.org/10.1016/j.bej.2022.108627openalex-snapshot
Selection of green fluorescent proteins by in vitro compartmentalization using microbead-display libraries
inferred← citeshttps://doi.org/10.1016/j.bej.2023.109036openalex-snapshot
Establishment of picodroplet-based co-culture system to improve erythritol production in Yarrowia lipolytica
inferred← citeshttps://doi.org/10.1016/j.biomaterials.2010.10.033openalex-snapshot
High-throughput generation of hydrogel microbeads with varying elasticity for cell encapsulation
inferred← citeshttps://doi.org/10.1016/j.biomaterials.2013.03.002openalex-snapshot
A programmable microenvironment for cellular studies via microfluidics-generated double emulsions
inferred← citeshttps://doi.org/10.1016/j.biomaterials.2015.02.042openalex-snapshot
Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development
inferred← citeshttps://doi.org/10.1016/j.biortech.2026.135204openalex-snapshot
Enzymatic depolymerization of polyurethanes: discovery, mechanisms, and engineering of hydrolases
inferred← citeshttps://doi.org/10.1016/j.bios.2013.03.078openalex-snapshot
High-throughput single-cell analysis of low copy number β-galactosidase by a laboratory-built high-sensitivity flow cytometer
inferred← citeshttps://doi.org/10.1016/j.bios.2014.08.059openalex-snapshot
Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes
inferred← citeshttps://doi.org/10.1016/j.bios.2014.11.001openalex-snapshot
Jetting microfluidics with size-sorting capability for single-cell protease detection
inferred← citeshttps://doi.org/10.1016/j.bios.2017.05.036openalex-snapshot
In-droplet cell concentration using dielectrophoresis
inferred← citeshttps://doi.org/10.1016/j.bios.2023.115213openalex-snapshot
High-throughput microfluidic droplets in biomolecular analytical system: A review
inferred← citeshttps://doi.org/10.1016/j.bios.2023.115972openalex-snapshot
Advanced strategies in high-throughput droplet screening for enzyme engineering
inferred← citeshttps://doi.org/10.1016/j.bios.2025.117376openalex-snapshot
Biosensor-based dual-color droplet microfluidic platform for precise high-throughput screening of erythromycin hyperproducers
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