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Activity and stability of α-chymotrypsin in biocompatible ionic liquids: enzyme refolding by triethyl ammonium acetate

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Enzyme-polyelectrolyte complexes in water-ethanol mixtures: Negatively charged groups artificially introduced into α-chymotrypsin provide additional activation and stabilization effects

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Ionic Liquids in Synthesis

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Diels–Alder Reactions Are Faster in Water than in Ionic Liquids at Room Temperature

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Understanding Ionic Liquids at the Molecular Level: Facts, Problems, and Controversies

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Protein Denaturation by Ionic Liquids and the Hofmeister Series: A Case Study of Aqueous Solutions of Ribonuclease A

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Stabilization of α‐chymotrypsin by ionic liquids in transesterification reactions

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Fluorescence and CD spectroscopic analysis of the α‐chymotrypsin stabilization by the ionic liquid, 1‐ethyl‐3‐methylimidazolium bis[(trifluoromethyl)sulfonyl]amide

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The structure of an insect chymotrypsin

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Secondary structural changes of chymotrypsinogen A, alpha-chymotrypsin, and the isolated polypeptides Cys1-Leu13, Ile16-Tyr146, and Ala149-Asn245 in sodium dodecyl sulfate, urea and guanidine hydrochloride

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Proteins. Structures and molecular properties

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The preparation and properties of two new chromogenic substrates of trypsin

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High resolution nuclear magnetic resonance study of the histidine—Aspartate hydrogen bond in chymotrypsin and chymotrypsinogen

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Structure of α-chymotrypsin refined at 1.68 Å resolution

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[16] The interpretation of near-ultraviolet circular dichroism

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Introduction to Protein Structure

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Trimethylamine N-oxide counteracts the denaturing effects of urea or GdnHCl on protein denatured state

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Small-angle neutron scattering studies of model protein denaturation in aqueous solutions of the ionic liquid 1-butyl-3-methylimidazolium chloride

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Influence of ionic liquids on the direct electrochemistry of glucose oxidase entrapped in nanogold-N,N-dimethylformamide-ionic liquid composite film

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Ionic liquids as refolding additives: Variation of the anion

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Protein Unfolding, and the “Tuning In” of Reversible Intermediate States, in Protic Ionic Liquid Media

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Properties of soluble α-chymotrypsin in neat glycerol and water

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Biocatalysis in nonaqueous solvents

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Direct Extraction of Double-Stranded DNA Into Ionic Liquid 1-Butyl-3-methylimidazolium Hexafluorophosphate and Its Quantification

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Structure and mechanism of chymotrypsin

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Unfolding free energy changes determined by the linear extrapolation method. 1. Unfolding of phenylmethanesulfonyl .alpha.-chymotrypsin using different denaturants

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Ionic Liquid-Reconstituted Cellulose Composites as Solid Support Matrices for Biocatalyst Immobilization

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Room-Temperature Ionic Liquids: Solvents for Synthesis and Catalysis. 2

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Room-Temperature Ionic Liquids. Solvents for Synthesis and Catalysis

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Thermodynamic Characterization of the Osmolyte Effect on Protein Stability and the Effect of GdnHCl on the Protein Denatured State

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Measurements and Molecular Interactions for N,N-Dimethylformamide with Ionic Liquid Mixed Solvents

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Temperature Effect on the Molecular Interactions between Ammonium Ionic Liquids and N,N-Dimethylformamide

inferredcites →https://doi.org/10.1021/jp8113013openalex-snapshot

Osmolyte Counteracts Urea-Induced Denaturation of α-Chymotrypsin

inferredcites →https://doi.org/10.1021/jp908498popenalex-snapshot

Contrasting Thermosolvatochromic Trends in Pyridinium-, Pyrrolidinium-, and Phosphonium-Based Ionic Liquids

inferredcites →https://doi.org/10.1021/jp9092332openalex-snapshot

Influence of Osmolytes and Denaturants on the Structure and Enzyme Activity of α-Chymotrypsin

inferredcites →https://doi.org/10.1039/b107270fopenalex-snapshot

Catalytic reactions in ionic liquids.

inferredcites →https://doi.org/10.1039/b618943aopenalex-snapshot

Reversible folding–unfolding, aggregation protection, and multi-year stabilization, in high concentration protein solutions, using ionic liquids

inferredcites →https://doi.org/10.1039/b900021fopenalex-snapshot

Activity and thermal stability of lysozyme in alkylammonium formate ionic liquids—influence of cation modification

inferredcites →https://doi.org/10.1110/ps.051596605openalex-snapshot

Ionic liquids as refolding additives: N′‐alkyl and N′‐(ω‐hydroxyalkyl) N‐methylimidazolium chlorides

inferredcites →https://doi.org/10.1110/ps.9.10.2001openalex-snapshot

Protein renaturation by the liquid organic salt ethylammonium nitrate

inferredcites →https://doi.org/10.1111/j.1432-1033.1994.tb19934.xopenalex-snapshot

Correlation of high‐temperature stability of α‐chymotrypsin with ‘salting‐in’ properties of solution

inferredcites →https://doi.org/10.1126/science.7112124openalex-snapshot

Living with Water Stress: Evolution of Osmolyte Systems

inferredcites →https://doi.org/10.1146/annurev.bb.11.060182.002223openalex-snapshot

Crystallographic and NMR Studies of the Serine Proteases

inferredcites →https://doi.org/10.1146/annurev.bi.46.070177.001555openalex-snapshot

Serine Proteases: Structure and Mechanism of Catalysis

inferredcites →https://doi.org/10.1246/cl.2003.450openalex-snapshot

Electron Transfer Process of Poly(ethylene oxide)-Modified Cytochrome c in Imidazolium Type Ionic Liquid

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Refolding of urea-induced denaturation of model proteins by trimethylamine N-oxide

inferred← citeshttps://doi.org/10.1016/j.tca.2012.07.004openalex-snapshot

Influence of temperature on thermophysical properties of ammonium ionic liquids with N-methyl-2-pyrrolidone

inferred← citeshttps://doi.org/10.1016/j.tca.2013.02.002openalex-snapshot

Effect of anion variation on the thermophysical properties of triethylammonium based protic ionic liquids with polar solvent

inferred← citeshttps://doi.org/10.1016/j.tca.2013.12.022openalex-snapshot

Effect of temperature on the interactions between low bandgap polymer and ionic liquids

inferred← citeshttps://doi.org/10.1016/j.tet.2014.02.025openalex-snapshot

Nanoscale organization of ionic liquids and their interaction with peptides probed by 13C NMR spectroscopy

inferred← citeshttps://doi.org/10.1016/j.trac.2018.09.008openalex-snapshot

Advances of ionic liquids-based methods for protein analysis

inferred← citeshttps://doi.org/10.1021/acs.biomac.6b00468openalex-snapshot

Lysozyme Solubility and Conformation in Neat Ionic Liquids and Their Mixtures with Water

inferred← citeshttps://doi.org/10.1021/acs.biomac.9b01556openalex-snapshot

Neat Ionic liquid and α‑Chymotrypsin-Polymer Surfactant Conjugate-Based Biocatalytic Solvent

inferred← citeshttps://doi.org/10.1021/acs.chemrev.3c00379openalex-snapshot

Ionic Liquids in Metal, Photo-, Electro-, and (Bio) Catalysis

inferred← citeshttps://doi.org/10.1021/acs.chemrev.3c00551openalex-snapshot

Do Ionic Liquids Exhibit the Required Characteristics to Dissolve, Extract, Stabilize, and Purify Proteins? Past-Present-Future Assessment

inferred← citeshttps://doi.org/10.1021/acs.chemrev.6b00562openalex-snapshot

Biological Activity of Ionic Liquids and Their Application in Pharmaceutics and Medicine

inferred← citeshttps://doi.org/10.1021/acs.iecr.1c02077openalex-snapshot

Phase and Chemical Equilibria of Biphasic Protic Ionic Liquid: Triethylamine–Acetic Acid

inferred← citeshttps://doi.org/10.1021/acs.iecr.5b01796openalex-snapshot

Effect of the Alkyl Chain Length of the Cation on the Interactions between Water and Ammonium-Based Ionic Liquids: Experimental and COSMO-RS Studies

inferred← citeshttps://doi.org/10.1021/acs.iecr.5c04649openalex-snapshot

Exploring the Amino Acids Behavior in Ionic Liquids: Thermophysical and Biophysical Approach

inferred← citeshttps://doi.org/10.1021/acs.jpcb.0c05633openalex-snapshot

On the Stability and Conformational Dynamics of Cytochrome c in Ammonium Ionic Liquids

inferred← citeshttps://doi.org/10.1021/acs.jpcb.5b04660openalex-snapshot

The Overriding Roles of Concentration and Hydrophobic Effect on Structure and Stability of Heme Protein Induced by Imidazolium-Based Ionic Liquids

inferred← citeshttps://doi.org/10.1021/acs.jpcb.6b02723openalex-snapshot

Role of Preferential Ions of Ammonium Ionic Liquid in Destabilization of Collagen

inferred← citeshttps://doi.org/10.1021/acs.jpcb.9b03562openalex-snapshot

Effect of Imidazolium-Based Ionic Liquids on the Structure and Phase Behavior of Palmitoyl-oleoyl-phosphatidylethanolamine

inferred← citeshttps://doi.org/10.1021/acs.jpcb.9b05466openalex-snapshot

Dynamics and Spectral Response of Water Molecules around Tetramethylammonium Cation

inferred← citeshttps://doi.org/10.1021/acs.molpharmaceut.8b00212openalex-snapshot

Dynamics of Ionic Liquid-Assisted Refolding of Denatured Cytochrome c: A Study of Preferential Interactions toward Renaturation

inferred← citeshttps://doi.org/10.1021/acsomega.8b03345openalex-snapshot

Development and Application of an Efficient Medium for Chromogenic Catalysis of Tetramethylbenzidine with Horseradish Peroxidase

inferred← citeshttps://doi.org/10.1021/acssensors.6b00250openalex-snapshot

Wearable Chemical Sensors: Present Challenges and Future Prospects

inferred← citeshttps://doi.org/10.1021/acssuschemeng.8b06201openalex-snapshot

Biological Impact of Ionic Liquids Based on Sustainable Fatty Acid Anions Examined with a Tripartite Test System

inferred← citeshttps://doi.org/10.1021/acssuschemeng.9b02151openalex-snapshot

Understanding Laccase–Ionic Liquid Interactions toward Biocatalytic Lignin Conversion in Aqueous Ionic Liquids

inferred← citeshttps://doi.org/10.1021/acssuschemeng.9b03741openalex-snapshot

Enhanced Activity of Immobilized Lipase by Phosphonium-Based Ionic Liquids Used in the Support Preparation and Immobilization Process

inferred← citeshttps://doi.org/10.1021/cr2003773openalex-snapshot

Overview of the Stability of α-Chymotrypsin in Different Solvent Media

inferred← citeshttps://doi.org/10.1021/ie300227fopenalex-snapshot

Free Energy of Transfer of a Methylene Group in Biphasic Systems of Water and Ionic Liquids [C3mpip][NTf2], [C3mpyrr][NTf2], and [C4mpyrr][NTf2]

inferred← citeshttps://doi.org/10.1021/je301170nopenalex-snapshot

ΔG(CH2) in Biphasic Systems of Water and Bis(trifluoromethylsulfonyl)Imide-Based Ionic Liquids

inferred← citeshttps://doi.org/10.1021/je500380xopenalex-snapshot

Density, Viscosity, and Conductivity of Binary Mixtures of the Ionic Liquid N-(2-Hydroxyethyl)piperazinium Propionate with Water, Methanol, or Ethanol

inferred← citeshttps://doi.org/10.1021/jp201826vopenalex-snapshot

Ionic Liquid Modifies the Lower Critical Solution Temperature (LCST) of Poly(N-isopropylacrylamide) in Aqueous Solution

inferred← citeshttps://doi.org/10.1021/jp2059084openalex-snapshot

Temperature Dependence Measurements and Structural Characterization of Trimethyl Ammonium Ionic Liquids with a Highly Polar Solvent

inferred← citeshttps://doi.org/10.1021/jp3015386openalex-snapshot

Influence of Alkyl Chain Length and Temperature on Thermophysical Properties of Ammonium-Based Ionic Liquids with Molecular Solvent

inferred← citeshttps://doi.org/10.1021/jp308443fopenalex-snapshot

Structural Basis for the Enhanced Stability of Protein Model Compounds and Peptide Backbone Unit in Ammonium Ionic Liquids

inferred← citeshttps://doi.org/10.1021/jp403813gopenalex-snapshot

Evaluation of Thermophysical Properties of Ionic Liquids with Polar Solvent: A Comparable Study of Two Families of Ionic Liquids with Various Ions

inferred← citeshttps://doi.org/10.1021/jp502400zopenalex-snapshot

Thermophysical Properties of Aqueous Solution of Ammonium-Based Ionic Liquids

inferred← citeshttps://doi.org/10.1021/la3017672openalex-snapshot

Quantifying Protein Adsorption and Function at Nanostructured Materials: Enzymatic Activity of Glucose Oxidase at GLAD Structured Electrodes

inferred← citeshttps://doi.org/10.1038/s41598-017-01030-wopenalex-snapshot

Structural and functional analysis of lysozyme after treatment with dielectric barrier discharge plasma and atmospheric pressure plasma jet

inferred← citeshttps://doi.org/10.1038/s41598-017-02133-0openalex-snapshot

A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)

inferred← citeshttps://doi.org/10.1038/s41598-017-02690-4openalex-snapshot

Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein

inferred← citeshttps://doi.org/10.1038/s41598-017-08643-1openalex-snapshot

The protective action of osmolytes on the deleterious effects of gamma rays and atmospheric pressure plasma on protein conformational changes

inferred← citeshttps://doi.org/10.1038/srep08221openalex-snapshot

Influence of reactive species on the modification of biomolecules generated from the soft plasma

inferred← citeshttps://doi.org/10.1038/srep17781openalex-snapshot

Influence of ionic liquid and ionic salt on protein against the reactive species generated using dielectric barrier discharge plasma

inferred← citeshttps://doi.org/10.1038/srep21779openalex-snapshot

Influence of plasma-activated compounds on melanogenesis and tyrosinase activity

inferred← citeshttps://doi.org/10.1038/srep35883openalex-snapshot

Variation in structure of proteins by adjusting reactive oxygen and nitrogen species generated from dielectric barrier discharge jet

inferred← citeshttps://doi.org/10.1039/c0cp02768eopenalex-snapshot

Thermodynamic characterization of the biocompatible ionic liquid effects on protein model compounds and their functional groups

inferred← citeshttps://doi.org/10.1039/c1cp22195gopenalex-snapshot

A protic ionic liquid attenuates the deleterious actions of urea on α-chymotrypsin

inferred← citeshttps://doi.org/10.1039/c2gc35197hopenalex-snapshot

Ionic liquids microemulsions: the key to Candida antarctica lipase B superactivity

inferred← citeshttps://doi.org/10.1039/c2ob26001hopenalex-snapshot

Water and a protic ionic liquid acted as refolding additives for chemically denatured enzymes

inferred← citeshttps://doi.org/10.1039/c2ra20085fopenalex-snapshot

TMAO and sorbitol attenuate the deleterious action of atmospheric pressure non-thermal jet plasma on α-chymotrypsin

inferred← citeshttps://doi.org/10.1039/c2ra22277aopenalex-snapshot

Prevention of insulin self-aggregation by a protic ionic liquid

inferred← citeshttps://doi.org/10.1039/c3cp54398fopenalex-snapshot

Unexpected effects of the alteration of structure and stability of myoglobin and hemoglobin in ammonium-based ionic liquids

inferred← citeshttps://doi.org/10.1039/c3ra44477eopenalex-snapshot

The stability of insulin in the presence of short alkyl chain imidazolium-based ionic liquids

inferred← citeshttps://doi.org/10.1039/c4cp01001aopenalex-snapshot

Quantitative evaluation of the ability of ionic liquids to offset the cold-induced unfolding of proteins

inferred← citeshttps://doi.org/10.1039/c4nj01596gopenalex-snapshot

A comparative study of the effects of the Hofmeister series anions of the ionic salts and ionic liquids on the stability of α-chymotrypsin

inferred← citeshttps://doi.org/10.1039/c4nj01741bopenalex-snapshot

Water-soluble complexes of an acrylamide copolymer and ionic liquids for inhibiting shale hydration

inferred← citeshttps://doi.org/10.1039/c4ra09434dopenalex-snapshot

Superactivity of α-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions

inferred← citeshttps://doi.org/10.1039/c5cp01735aopenalex-snapshot

Endeavour to simplify the frustrated concept of protein-ammonium family ionic liquid interactions

inferred← citeshttps://doi.org/10.1039/c5ra16317jopenalex-snapshot

Self-buffering and biocompatible ionic liquid based biological media for enzymatic research

inferred← citeshttps://doi.org/10.1039/c5ra26690dopenalex-snapshot

Exploring the structure and stability of amino acids and glycine peptides in biocompatible ionic liquids

inferred← citeshttps://doi.org/10.1039/c6cp00199hopenalex-snapshot

Molecular interactions between ammonium-based ionic liquids and molecular solvents: current progress and challenges

inferred← citeshttps://doi.org/10.1039/c6cp01022aopenalex-snapshot

Refolding effects of partially immiscible ammonium-based ionic liquids on the urea-induced unfolded lysozyme structure

inferred← citeshttps://doi.org/10.1039/c6cp05080hopenalex-snapshot

The temperature dependence of the Hofmeister series: thermodynamic fingerprints of cosolute–protein interactions

inferred← citeshttps://doi.org/10.1039/c6me00047aopenalex-snapshot

Kinetics and mechanism of ionic-liquid induced protein unfolding: application to the model protein HP35

inferred← citeshttps://doi.org/10.1039/c6ra00607hopenalex-snapshot

Extraction of an active enzyme by self-buffering ionic liquids: a green medium for enzymatic research

inferred← citeshttps://doi.org/10.1039/c6ra11833jopenalex-snapshot

Counteraction of lactose on the thermal stability and activity of α-chymotrypsin: thermodynamic, kinetic and docking studies

inferred← citeshttps://doi.org/10.1039/c6ra28483copenalex-snapshot

Homogeneous capture and heterogeneous separation of proteins by PEG-functionalized ionic liquid–water systems

inferred← citeshttps://doi.org/10.1039/c7cp04083kopenalex-snapshot

Impact of an ionic liquid on protein thermodynamics in the presence of cold atmospheric plasma and gamma rays

inferred← citeshttps://doi.org/10.1039/c7gc00705aopenalex-snapshot

An economically viable ionic liquid for the fractionation of lignocellulosic biomass

inferred← citeshttps://doi.org/10.1039/c7nj02900dopenalex-snapshot

A spectroscopic and molecular dynamics simulation approach towards the stabilizing effect of ammonium-based ionic liquids on bovine serum albumin

inferred← citeshttps://doi.org/10.1039/c7nj03023aopenalex-snapshot

Influence of cholinium-based ionic liquids on the structural stability and activity of α-chymotrypsin

inferred← citeshttps://doi.org/10.1039/c9cp04772gopenalex-snapshot

Strategic planning of proteins in ionic liquids: future solvents for the enhanced stability of proteins against multiple stresses

inferred← citeshttps://doi.org/10.1039/d0gc02387fopenalex-snapshot

Biocompatible ionic liquids and their applications in pharmaceutics

inferred← citeshttps://doi.org/10.1039/d2cp00608aopenalex-snapshot

Recovery of enzyme structure and activity following rehydration from ionic liquid

inferred← citeshttps://doi.org/10.1039/d3cp05607dopenalex-snapshot

Opposing roles of organic salts on mini-protein structure

inferred← citeshttps://doi.org/10.1080/00387010.2016.1167089openalex-snapshot

Impact of imidazolium-based ionic liquids on the structure and stability of lysozyme

inferred← citeshttps://doi.org/10.1080/07391102.2018.1495578openalex-snapshot

Molecular modelling and vibrational investigations of ammonium-based ionic liquid (CLTOAB)

inferred← citeshttps://doi.org/10.1134/s1070428024060150openalex-snapshot

Ultrasound-Assisted Synthesis and DFT Studies of Novel Schiff Bases Derived from 3-Methyl-1,3-benzothiazol-2(3H)-one in an Ionic Liquid Medium

inferred← citeshttps://doi.org/10.1155/2015/461974openalex-snapshot

Interaction Studies between Newly Synthesized Photosensitive Polymer and Ionic Liquids

inferred← citeshttps://doi.org/10.1155/2018/8275985openalex-snapshot

Effect of Ionic Liquids on the Physical Properties of the Newly Synthesized Conducting Polymer

inferred← citeshttps://doi.org/10.12925/jkocs.2014.31.3.337openalex-snapshot

Interaction of Conjugated Conducting Polymer with Ionic Liquids

inferred← citeshttps://doi.org/10.1371/journal.pone.0068970openalex-snapshot

Elucidating Interactions and Conductivity of Newly Synthesised Low Bandgap Polymer with Protic and Aprotic Ionic Liquids

inferred← citeshttps://doi.org/10.1371/journal.pone.0086530openalex-snapshot

Influence of Hydroxyl Group Position and Temperature on Thermophysical Properties of Tetraalkylammonium Hydroxide Ionic Liquids with Alcohols

inferred← citeshttps://doi.org/10.18433/jpps30965openalex-snapshot

Potential Application of Ionic Liquids in Pharmaceutical Dosage Forms for Small Molecule Drug and Vaccine Delivery System

inferred← citeshttps://doi.org/10.2174/1385272824999200801022221openalex-snapshot

Ionic Liquid Assisted C-C Bond Formation

inferred← citeshttps://doi.org/10.2174/1573406415666190319101329openalex-snapshot

Novel Amide Derivatives as Potent Tyrosinase Inhibitors; In-vitro, In-vivo Antimelanogenic Activity and Computational Studies

inferred← citeshttps://doi.org/10.2174/2212711901666131224222431openalex-snapshot

Thermodynamic Contribution of Amino Acids in Ionic Liquids Towards Protein Stability

inferred← citeshttps://doi.org/10.2210/pdb6jcg/pdbopenalex-snapshot

Room temperature structure of HIV-1 Integrase catalytic core domain by serial femtosecond crystallography.

inferred← citeshttps://doi.org/10.3389/fchem.2019.00074openalex-snapshot

FTIR Spectroscopic Study of the Secondary Structure of Globular Proteins in Aqueous Protic Ionic Liquids

inferred← citeshttps://doi.org/10.3390/biom9070264openalex-snapshot

Effects of Ionic Liquid Alkyl Chain Length on Denaturation of Myoglobin by Anionic, Cationic, and Zwitterionic Detergents

inferred← citeshttps://doi.org/10.3390/biomedicines11113093openalex-snapshot

The Potential Role of Ionic Liquid as a Multifunctional Dental Biomaterial

inferred← citeshttps://doi.org/10.3390/ijms20081943openalex-snapshot

Non-Cryogenic Structure and Dynamics of HIV-1 Integrase Catalytic Core Domain by X-ray Free-Electron Lasers

inferred← citeshttps://doi.org/10.3390/ma18112476openalex-snapshot

Advancements in the Research on the Preparation of Isoamyl Acetate Catalyzed by Immobilized Lipase

inferred← citeshttps://doi.org/10.3390/molecules27030929openalex-snapshot

Enhanced Enzyme Reuse through the Bioconjugation of L-Asparaginase and Silica-Based Supported Ionic Liquid-like Phase Materials

inferred← citeshttps://doi.org/10.3390/molecules30071574openalex-snapshot

Cholinium-Based Ionic Liquids Modulate Protein Stability: A Comparative Study of Enzymes and Albumins

inferred← citeshttps://doi.org/10.3390/suschem1020010openalex-snapshot

Recent Trends in Processing of Proteins and DNA in Alternative Solvents: A Sustainable Approach

inferred← citeshttps://doi.org/10.3938/jkps.60.959openalex-snapshot

Effects of atmospheric-pressure non-thermal plasma jets on enzyme solutions

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