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BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis synthetic biology

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/bit.22003openalex-snapshot
Fermentative butanol production by clostridia
inferredcites →https://doi.org/10.1002/j.1460-2075.1993.tb06004.xopenalex-snapshot
In vivo reactivation of catechol 2,3‐dioxygenase mediated by a chloroplast‐type ferredoxin: a bacterial strategy to expand the substrate specificity of aromatic degradative pathways.
inferredcites →https://doi.org/10.1002/pmic.201300183openalex-snapshot
Proteomic analysis of Bacillus subtilis strains engineered for improved production of heterologous proteins
inferredcites →https://doi.org/10.1006/jmbi.1999.2624openalex-snapshot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems
inferredcites →https://doi.org/10.1007/978-3-642-37916-1_33openalex-snapshot
Elimination of Carbon Catabolite Repression in Bacillus subtilis for the Improvement of 2,3-Butanediol Production
inferredcites →https://doi.org/10.1007/s00253-016-8080-9openalex-snapshot
Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future
inferredcites →https://doi.org/10.1007/s10529-016-2194-0openalex-snapshot
Artificial induction of genetic competence in Bacillus amyloliquefaciens isolates
inferredcites →https://doi.org/10.1016/j.jmb.2006.07.034openalex-snapshot
Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria
inferredcites →https://doi.org/10.1016/j.mib.2016.07.011openalex-snapshot
Functional requirements of cellular differentiation: lessons from Bacillus subtilis
inferredcites →https://doi.org/10.1016/j.tim.2007.12.001openalex-snapshot
Bacillus protein secretion: an unfolding story
inferredcites →https://doi.org/10.1021/acssynbio.6b00031openalex-snapshot
EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology
inferredcites →https://doi.org/10.1021/acssynbio.6b00065openalex-snapshot
Effect of Genome Position on Heterologous Gene Expression in Bacillus subtilis: An Unbiased Analysis
inferredcites →https://doi.org/10.1038/36786openalex-snapshot
The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
inferredcites →https://doi.org/10.1039/c3mb25568aopenalex-snapshot
In silico metabolic engineering of Bacillus subtilis for improved production of riboflavin, Egl-237, ( R , R )-2,3-butanediol and isobutanol
inferredcites →https://doi.org/10.1073/pnas.44.10.1072openalex-snapshot
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE
inferredcites →https://doi.org/10.1073/pnas.86.7.2172openalex-snapshot
One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.
inferredcites →https://doi.org/10.1073/pnas.93.20.10891openalex-snapshot
NOMAD: a versatile strategy for in vitro DNA manipulation applied to promoter analysis and vector design.
inferredcites →https://doi.org/10.1074/jbc.m114.577924openalex-snapshot
Roles of Bacillus subtilis DprA and SsbA in RecA-mediated Genetic Recombination
inferredcites →https://doi.org/10.1093/jb/mvg062openalex-snapshot
Transcriptional, Functional and Cytochemical Analyses of the veg Gene in Bacillus subtilis
inferredcites →https://doi.org/10.1093/nar/gkv545openalex-snapshot
Bacillus subtilisRecO and SsbA are crucial for RecA-mediated recombinational DNA repair
inferredcites →https://doi.org/10.1093/nar/gkw624openalex-snapshot
A part toolbox to tune genetic expression inBacillus subtilis
inferredcites →https://doi.org/10.1099/jmm.0.000404openalex-snapshot
Recombinant Bacillus subtilis spores expressing cholera toxin B subunit and Helicobacter pylori urease B confer protection against H. pylori in mice
inferredcites →https://doi.org/10.1111/j.1365-2958.1991.tb00728.xopenalex-snapshot
Catabolite repression of α amylase gene expression in Bacillus subtilis involves a trans‐acting gene product homologous to the Escherichia coli lacl and galR repressors
inferredcites →https://doi.org/10.1128/aem.69.11.6841-6847.2003openalex-snapshot
Peptide Ligands That Bind Selectively to Spores of Bacillus subtilis and Closely Related Species
inferredcites →https://doi.org/10.1128/aem.72.4.2520-2525.2006openalex-snapshot
An Efficient Method of Selectable Marker Gene Excision by Xer Recombination for Gene Replacement in Bacterial Chromosomes
inferredcites →https://doi.org/10.1128/jb.00310-06openalex-snapshot
Regulation of LiaRS-Dependent Gene Expression inBacillus subtilis: Identification of Inhibitor Proteins, Regulator Binding Sites, and Target Genes of a Conserved Cell Envelope Stress-Sensing Two-Component System
inferredcites →https://doi.org/10.1128/jb.173.16.4952-4958.1991openalex-snapshot
Engineering a Bacillus subtilis expression-secretion system with a strain deficient in six extracellular proteases
inferredcites →https://doi.org/10.1128/jb.180.7.1655-1661.1998openalex-snapshot
The ars Operon in the skin Element of Bacillus subtilis Confers Resistance to Arsenate and Arsenite
inferredcites →https://doi.org/10.1128/mmbr.55.3.395-424.1991openalex-snapshot
Genetic competence in Bacillus subtilis
inferredcites →https://doi.org/10.1139/w03-076openalex-snapshot
Developments in the use of Bacillus species for industrial production
inferredcites →https://doi.org/10.1139/w09-044openalex-snapshot
Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter
inferredcites →https://doi.org/10.1146/annurev.micro.55.1.647openalex-snapshot
Anthrax
inferredcites →https://doi.org/10.1186/1475-2859-12-3openalex-snapshot
Bacillus subtilis: from soil bacterium to super-secreting cell factory
inferredcites →https://doi.org/10.1186/1475-2859-12-6openalex-snapshot
Repressible promoters – A novel tool to generate conditional mutants in Pichia pastoris
inferredcites →https://doi.org/10.1186/1754-1611-7-16openalex-snapshot
Hidden hysteresis – population dynamics can obscure gene network dynamics
inferredcites →https://doi.org/10.1186/1754-1611-7-29openalex-snapshot
The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis
inferredcites →https://doi.org/10.1371/journal.pone.0003582openalex-snapshot
Evolutionary Changes in the Complexity of the Tectum of Nontetrapods: A Cladistic Approach
inferredcites →https://doi.org/10.1371/journal.pone.0003647openalex-snapshot
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
inferredcites →https://doi.org/10.1371/journal.pone.0087454openalex-snapshot
Bacillus subtilis Spores as Vaccine Adjuvants: Further Insights into the Mechanisms of Action
inferredcites →https://doi.org/10.1371/journal.pone.0095449openalex-snapshot
Evolution of Bacterial Consortia in Spontaneously Started Rye Sourdoughs during Two Months of Daily Propagation
inferredcites →https://doi.org/10.2471/blt.11.101253openalex-snapshot
Arsenic in tube well water in Bangladesh: health and economic impacts and implications for arsenic mitigation
inferredcites →https://doi.org/10.3109/08830180903215605openalex-snapshot
Oral Vaccine Delivery by Recombinant Spore Probiotics
inferredcites →https://doi.org/10.4161/hv.20694openalex-snapshot
Bacillus subtilis

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1007/s00253-026-13886-yopenalex-snapshot
Evaluation of biobased carriers derived from agri-food waste for biostimulants delivery in horticulture
inferred← citeshttps://doi.org/10.1016/j.tibtech.2025.02.004openalex-snapshot
SubtiToolKit: a bioengineering kit for Bacillus subtilis and Gram-positive bacteria
inferred← citeshttps://doi.org/10.1021/acssynbio.1c00126openalex-snapshot
The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens
inferred← citeshttps://doi.org/10.1021/acssynbio.1c00358openalex-snapshot
Komagataeibacter Tool Kit (KTK): A Modular Cloning System for Multigene Constructs and Programmed Protein Secretion from Cellulose Producing Bacteria
inferred← citeshttps://doi.org/10.1101/2025.09.09.675032openalex-snapshot
Development of a Toolkit for High-Efficiency, Markerless, and Iterative Genome Editing in Shouchella clausii
inferred← citeshttps://doi.org/10.1101/395178openalex-snapshot
A simple chromogenic whole-cell arsenic biosensor based on Bacillus subtilis
inferred← citeshttps://doi.org/10.1111/1751-7915.70287openalex-snapshot
Development of a Tool for High‐Efficiency, Markerless and Iterative Genome Editing in Shouchella clausii
inferred← citeshttps://doi.org/10.1186/s13068-020-01872-zopenalex-snapshot
Approaches to genetic tool development for rapid domestication of non-model microorganisms
inferred← citeshttps://doi.org/10.3390/applmicrobiol6010019openalex-snapshot
Practical Insights and Emerging Trends for Strategic Cloning of Large Biosynthetic Gene Clusters from Bacteria
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