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Engineering a promiscuous pyrrolysyl-tRNA synthetase by a high throughput FACS screen

https://doi.org/10.1101/229054
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Outgoing claims: the papers this work cites, builds on, or argues with.

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High‐Throughput Screening for Methionyl‐tRNA Synthetases That Enable Residue‐Specific Incorporation of Noncanonical Amino Acids into Recombinant Proteins in Bacterial Cells
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Amino Acids for Diels–Alder Reactions in Living Cells
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Minimal Tags for Rapid Dual‐Color Live‐Cell Labeling and Super‐Resolution Microscopy
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Addressing the Numbers Problem in Directed Evolution
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Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins
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Structural Basis of Furan–Amino Acid Recognition by a Polyspecific Aminoacyl‐tRNA‐Synthetase and its Genetic Encoding in Human Cells
inferredcites →https://doi.org/10.1002/cbic.201700039openalex-snapshot
Two‐Tier Screening Platform for Directed Evolution of Aminoacyl–tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency
inferredcites →https://doi.org/10.1002/chin.201147190openalex-snapshot
ChemInform Abstract: Highly Efficient Carbamate Formation from Alcohols and Hindered Amino Acids or Esters Using N,N′‐Disuccinimidyl Carbonate (DSC).
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A point‐charge force field for molecular mechanics simulations of proteins based on condensed‐phase quantum mechanical calculations
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New ways to boost molecular dynamics simulations
inferredcites →https://doi.org/10.1016/j.bbapap.2014.03.002openalex-snapshot
Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool
inferredcites →https://doi.org/10.1016/j.cbpa.2011.03.007openalex-snapshot
The pyrrolysine translational machinery as a genetic-code expansion tool
inferredcites →https://doi.org/10.1016/j.chembiol.2008.10.004openalex-snapshot
Multistep Engineering of Pyrrolysyl-tRNA Synthetase to Genetically Encode Nɛ-(o-Azidobenzyloxycarbonyl) lysine for Site-Specific Protein Modification
inferredcites →https://doi.org/10.1016/j.febslet.2006.11.028openalex-snapshot
Pyrrolysine analogues as substrates for pyrrolysyl‐tRNA synthetase
inferredcites →https://doi.org/10.1016/j.jmb.2009.10.030openalex-snapshot
An Enhanced System for Unnatural Amino Acid Mutagenesis in E. coli
inferredcites →https://doi.org/10.1016/j.jmb.2010.09.001openalex-snapshot
Engineering of an Orthogonal Aminoacyl-tRNA Synthetase for Efficient Incorporation of the Non-natural Amino Acid O-Methyl-L-tyrosine using Fluorescence-based Bacterial Cell Sorting
inferredcites →https://doi.org/10.1021/bi901947ropenalex-snapshot
Generating Permissive Site-Specific Unnatural Aminoacyl-tRNA Synthetases
inferredcites →https://doi.org/10.1021/cb200057aopenalex-snapshot
Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids
inferredcites →https://doi.org/10.1021/cr400355wopenalex-snapshot
Cellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
inferredcites →https://doi.org/10.1021/ja104609mopenalex-snapshot
Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair
inferredcites →https://doi.org/10.1021/ja203111copenalex-snapshot
Genetically Encoded 1,2-Aminothiols Facilitate Rapid and Site-Specific Protein Labeling via a Bio-orthogonal Cyanobenzothiazole Condensation
inferredcites →https://doi.org/10.1021/ja302832gopenalex-snapshot
Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions
inferredcites →https://doi.org/10.1021/ja411535qopenalex-snapshot
A Genetically Encoded Spin Label for Electron Paramagnetic Resonance Distance Measurements
inferredcites →https://doi.org/10.1021/ja5055862openalex-snapshot
Genetically Encoded Optochemical Probes for Simultaneous Fluorescence Reporting and Light Activation of Protein Function with Two-Photon Excitation
inferredcites →https://doi.org/10.1038/nbt742openalex-snapshot
An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
inferredcites →https://doi.org/10.1038/nchembio.73openalex-snapshot
Genetically encoding Nε-acetyllysine in recombinant proteins
inferredcites →https://doi.org/10.1038/nmeth.1318openalex-snapshot
Enzymatic assembly of DNA molecules up to several hundred kilobases
inferredcites →https://doi.org/10.1038/nrg3927openalex-snapshot
Methods for the directed evolution of proteins
inferredcites →https://doi.org/10.1038/nrm2005openalex-snapshot
A chemical toolkit for proteins — an expanded genetic code
inferredcites →https://doi.org/10.1039/b909200popenalex-snapshot
Incorporation of unnatural amino acids for synthetic biology
inferredcites →https://doi.org/10.1039/c0mb00217hopenalex-snapshot
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of l -phenylalanine and its derivatives
inferredcites →https://doi.org/10.1039/c4sc01534gopenalex-snapshot
Designing logical codon reassignment – Expanding the chemistry in biology
inferredcites →https://doi.org/10.1042/bj20060874openalex-snapshot
Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging
inferredcites →https://doi.org/10.1055/s-0030-1260584openalex-snapshot
Highly Efficient Carbamate Formation from Alcohols and Hindered Amino Acids or Esters Using N,N′-Disuccinimidyl Carbonate (DSC)
inferredcites →https://doi.org/10.1073/pnas.0234824100openalex-snapshot
Addition of the keto functional group to the genetic code of Escherichia coli
inferredcites →https://doi.org/10.1073/pnas.0601167103openalex-snapshot
Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids
inferredcites →https://doi.org/10.1073/pnas.0704769104openalex-snapshot
Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation
inferredcites →https://doi.org/10.1073/pnas.1419737111openalex-snapshot
Polyspecific pyrrolysyl-tRNA synthetases from directed evolution
inferredcites →https://doi.org/10.1093/bioinformatics/bti516openalex-snapshot
Statistics of protein library construction
inferredcites →https://doi.org/10.1126/science.1060077openalex-snapshot
Expanding the Genetic Code of Escherichia coli
inferredcites →https://doi.org/10.1126/science.1131943openalex-snapshot
Fluorine in Pharmaceuticals: Looking Beyond Intuition
inferredcites →https://doi.org/10.1146/annurev.biochem.052308.105824openalex-snapshot
Adding New Chemistries to the Genetic Code
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