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scRNA-seq mixology: towards better benchmarking of single cell RNA-seq analysis methods

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1007/bf01908075openalex-snapshot
Comparing partitions
inferredcites →https://doi.org/10.1016/j.cell.2015.04.044openalex-snapshot
Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells
inferredcites →https://doi.org/10.1016/j.cell.2015.05.002openalex-snapshot
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
inferredcites →https://doi.org/10.1016/j.cels.2016.09.002openalex-snapshot
A Single-Cell Transcriptome Atlas of the Human Pancreas
inferredcites →https://doi.org/10.1016/j.cels.2019.03.010openalex-snapshot
Performance Assessment and Selection of Normalization Procedures for Single-Cell RNA-Seq
inferredcites →https://doi.org/10.1038/nbt.2957openalex-snapshot
A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequencing Quality Control Consortium
inferredcites →https://doi.org/10.1038/nbt.3192openalex-snapshot
Spatial reconstruction of single-cell gene expression data
inferredcites →https://doi.org/10.1038/nbt.4042openalex-snapshot
Multiplexed droplet single-cell RNA-sequencing using natural genetic variation
inferredcites →https://doi.org/10.1038/nbt.4091openalex-snapshot
Batch effects in single-cell RNA-sequencing data are corrected by matching mutual nearest neighbors
inferredcites →https://doi.org/10.1038/nbt.4096openalex-snapshot
Integrating single-cell transcriptomic data across different conditions, technologies, and species
inferredcites →https://doi.org/10.1038/ng.3818openalex-snapshot
Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors
inferredcites →https://doi.org/10.1038/nmeth.2645openalex-snapshot
Accounting for technical noise in single-cell RNA-seq experiments
inferredcites →https://doi.org/10.1038/nmeth.2930openalex-snapshot
Validation of noise models for single-cell transcriptomics
inferredcites →https://doi.org/10.1038/nmeth.3971openalex-snapshot
Diffusion pseudotime robustly reconstructs lineage branching
inferredcites →https://doi.org/10.1038/nmeth.4220openalex-snapshot
Power analysis of single-cell RNA-sequencing experiments
inferredcites →https://doi.org/10.1038/nmeth.4236openalex-snapshot
SC3: consensus clustering of single-cell RNA-seq data
inferredcites →https://doi.org/10.1038/nmeth.4263openalex-snapshot
SCnorm: robust normalization of single-cell RNA-seq data
inferredcites →https://doi.org/10.1038/nmeth.4402openalex-snapshot
Reversed graph embedding resolves complex single-cell trajectories
inferredcites →https://doi.org/10.1038/nmeth.4612openalex-snapshot
Bias, robustness and scalability in single-cell differential expression analysis
inferredcites →https://doi.org/10.1038/nmeth.4662openalex-snapshot
FateID infers cell fate bias in multipotent progenitors from single-cell RNA-seq data
inferredcites →https://doi.org/10.1038/s41586-018-0590-4openalex-snapshot
Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
inferredcites →https://doi.org/10.1038/s41587-019-0071-9openalex-snapshot
A comparison of single-cell trajectory inference methods
inferredcites →https://doi.org/10.1038/s41587-019-0113-3openalex-snapshot
Efficient integration of heterogeneous single-cell transcriptomes using Scanorama
inferredcites →https://doi.org/10.1038/s41592-018-0254-1openalex-snapshot
A test metric for assessing single-cell RNA-seq batch correction
inferredcites →https://doi.org/10.1038/srep39921openalex-snapshot
Batch effects and the effective design of single-cell gene expression studies
inferredcites →https://doi.org/10.1073/pnas.1820006116openalex-snapshot
scMerge leverages factor analysis, stable expression, and pseudoreplication to merge multiple single-cell RNA-seq datasets
inferredcites →https://doi.org/10.1093/bib/bby011openalex-snapshot
Evaluation of tools for highly variable gene discovery from single-cell RNA-seq data
inferredcites →https://doi.org/10.1093/bioinformatics/btg410openalex-snapshot
A benchmark for Affymetrix GeneChip expression measures
inferredcites →https://doi.org/10.1093/bioinformatics/btp616openalex-snapshot
edgeR : a Bioconductor package for differential expression analysis of digital gene expression data
inferredcites →https://doi.org/10.1093/gigascience/giy083openalex-snapshot
Clustering trees: a visualization for evaluating clusterings at multiple resolutions
inferredcites →https://doi.org/10.1093/nar/gkt214openalex-snapshot
The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote
inferredcites →https://doi.org/10.1093/nar/gkw1046openalex-snapshot
Division of labor among Mycobacterium smegmatis RNase H enzymes: RNase H1 activity of RnhA or RnhC is essential for growth whereas RnhB and RnhA guard against killing by hydrogen peroxide in stationary phase
inferredcites →https://doi.org/10.1093/nar/gkw430openalex-snapshot
TSCAN: Pseudo-time reconstruction and evaluation in single-cell RNA-seq analysis
inferredcites →https://doi.org/10.1093/nar/gkx828openalex-snapshot
Linnorm: improved statistical analysis for single cell RNA-seq expression data
inferredcites →https://doi.org/10.1101/138677openalex-snapshot
SAVER: Gene expression recovery for UMI-based single cell RNA sequencing
inferredcites →https://doi.org/10.1101/175927openalex-snapshot
scPipe: a flexible R/Bioconductor preprocessing pipeline for single-cell RNA-sequencing data
inferredcites →https://doi.org/10.1101/217737openalex-snapshot
K-nearest neighbor smoothing for high-throughput single-cell RNA-Seq data
inferredcites →https://doi.org/10.1101/gr.121095.111openalex-snapshot
Synthetic spike-in standards for RNA-seq experiments
inferredcites →https://doi.org/10.1186/gb-2010-11-3-r25openalex-snapshot
A scaling normalization method for differential expression analysis of RNA-seq data
inferredcites →https://doi.org/10.1186/s12859-017-1553-8openalex-snapshot
MINT: a multivariate integrative method to identify reproducible molecular signatures across independent experiments and platforms
inferredcites →https://doi.org/10.1186/s12859-018-2226-yopenalex-snapshot
DrImpute: imputing dropout events in single cell RNA sequencing data
inferredcites →https://doi.org/10.1186/s12864-018-4772-0openalex-snapshot
Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics
inferredcites →https://doi.org/10.1186/s13059-014-0550-8openalex-snapshot
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
inferredcites →https://doi.org/10.1186/s13059-016-0938-8openalex-snapshot
CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
inferredcites →https://doi.org/10.1186/s13059-016-0947-7openalex-snapshot
Pooling across cells to normalize single-cell RNA sequencing data with many zero counts
inferredcites →https://doi.org/10.1186/s13059-016-0975-3openalex-snapshot
SLICER: inferring branched, nonlinear cellular trajectories from single cell RNA-seq data
inferredcites →https://doi.org/10.12688/f1000research.15666.1openalex-snapshot
A systematic performance evaluation of clustering methods for single-cell RNA-seq data
inferredcites →https://doi.org/10.12688/f1000research.15809.1openalex-snapshot
Comparison of clustering tools in R for medium-sized 10x Genomics single-cell RNA-sequencing data
inferredcites →https://doi.org/10.12688/f1000research.16613.2openalex-snapshot
False signals induced by single-cell imputation
inferredcites →https://doi.org/10.1371/journal.pcbi.1004333openalex-snapshot
BASiCS: Bayesian Analysis of Single-Cell Sequencing Data
inferredcites →https://doi.org/10.1371/journal.pcbi.1005752openalex-snapshot
mixOmics: An R package for ‘omics feature selection and multiple data integration

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1038/s41587-019-0071-9openalex-snapshot
A comparison of single-cell trajectory inference methods
inferred← citeshttps://doi.org/10.1038/s41592-019-0529-1openalex-snapshot
Probabilistic cell-type assignment of single-cell RNA-seq for tumor microenvironment profiling
inferred← citeshttps://doi.org/10.1038/s41592-019-0654-xopenalex-snapshot
Orchestrating single-cell analysis with Bioconductor
inferred← citeshttps://doi.org/10.1073/pnas.1820006116openalex-snapshot
scMerge leverages factor analysis, stable expression, and pseudoreplication to merge multiple single-cell RNA-seq datasets
inferred← citeshttps://doi.org/10.1101/2022.04.26.489628openalex-snapshot
Projecting clumped transcriptomes onto single cell atlases to achieve single cell resolution
inferred← citeshttps://doi.org/10.1186/s13059-019-1766-4openalex-snapshot
scAlign: a tool for alignment, integration, and rare cell identification from scRNA-seq data
inferred← citeshttps://doi.org/10.1186/s13059-020-02096-yopenalex-snapshot
Demystifying “drop-outs” in single-cell UMI data
inferred← citeshttps://doi.org/10.1186/s13059-020-02136-7openalex-snapshot
pipeComp, a general framework for the evaluation of computational pipelines, reveals performant single cell RNA-seq preprocessing tools
inferred← citeshttps://doi.org/10.12688/f1000research.15809.1openalex-snapshot
Comparison of clustering tools in R for medium-sized 10x Genomics single-cell RNA-sequencing data
inferred← citeshttps://doi.org/10.12688/f1000research.73600.1openalex-snapshot
Doublet identification in single-cell sequencing data using scDblFinder
inferred← citeshttps://doi.org/10.12688/f1000research.73600.2openalex-snapshot
Doublet identification in single-cell sequencing data using scDblFinder
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