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Universal Loop assembly (uLoop): open, efficient, and species-agnostic DNA fabrication

https://doi.org/10.1101/744854
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1007/978-1-4939-0799-1_4openalex-snapshot
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
inferredcites →https://doi.org/10.1021/acssynbio.5b00124openalex-snapshot
CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology
inferredcites →https://doi.org/10.1021/acssynbio.8b00060openalex-snapshot
Single Day Construction of Multigene Circuits with 3G Assembly
inferredcites →https://doi.org/10.1021/acssynbio.8b00251openalex-snapshot
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
inferredcites →https://doi.org/10.1021/acssynbio.9b00322openalex-snapshot
A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
inferredcites →https://doi.org/10.1021/sb400055hopenalex-snapshot
Visualization of Evolutionary Stability Dynamics and Competitive Fitness of Escherichia coli Engineered with Randomized Multigene Circuits
inferredcites →https://doi.org/10.1021/sb4001504openalex-snapshot
A Golden Gate Modular Cloning Toolbox for Plants
inferredcites →https://doi.org/10.1021/sb500366vopenalex-snapshot
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
inferredcites →https://doi.org/10.1038/nbt.4263openalex-snapshot
Opening options for material transfer
inferredcites →https://doi.org/10.1038/ncomms7925openalex-snapshot
Designer diatom episomes delivered by bacterial conjugation
inferredcites →https://doi.org/10.1038/nmeth.1318openalex-snapshot
Enzymatic assembly of DNA molecules up to several hundred kilobases
inferredcites →https://doi.org/10.1038/nprot.2007.15openalex-snapshot
Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
inferredcites →https://doi.org/10.1038/nprot.2007.199openalex-snapshot
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis
inferredcites →https://doi.org/10.1038/nrm4014openalex-snapshot
Bricks and blueprints: methods and standards for DNA assembly
inferredcites →https://doi.org/10.1038/s41598-019-46171-2openalex-snapshot
A unified multi-kingdom Golden Gate cloning platform
inferredcites →https://doi.org/10.1039/c0ib00070aopenalex-snapshot
DNA assembly for synthetic biology: from parts to pathways and beyond
inferredcites →https://doi.org/10.1073/pnas.97.7.3718openalex-snapshot
Random GFP∷cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency
inferredcites →https://doi.org/10.1093/nar/gkp448openalex-snapshot
Gene silencing in the marine diatom Phaeodactylum tricornutum
inferredcites →https://doi.org/10.1093/nar/gkt860openalex-snapshot
Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly
inferredcites →https://doi.org/10.1093/nar/gky596openalex-snapshot
MetClo: methylase-assisted hierarchical DNA assembly using a single type IIS restriction enzyme
inferredcites →https://doi.org/10.1101/cshperspect.a016147openalex-snapshot
The Eukaryotic Tree of Life from a Global Phylogenomic Perspective
inferredcites →https://doi.org/10.1111/1567-1364.12171openalex-snapshot
Recent advances in DNA assembly technologies
inferredcites →https://doi.org/10.1111/j.1365-313x.2010.04322.xopenalex-snapshot
A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies
inferredcites →https://doi.org/10.1111/nph.13532openalex-snapshot
Standards for plant synthetic biology: a common syntax for exchange of DNA parts
inferredcites →https://doi.org/10.1111/nph.15625openalex-snapshot
Loop assembly: a simple and open system for recursive fabrication of DNA circuits
inferredcites →https://doi.org/10.1126/science.aac7341openalex-snapshot
Genetic circuit design automation
inferredcites →https://doi.org/10.1186/1475-2859-12-60openalex-snapshot
Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci
inferredcites →https://doi.org/10.1186/1754-1611-2-5openalex-snapshot
Engineering BioBrick vectors from BioBrick parts
inferredcites →https://doi.org/10.1242/jcs.00092openalex-snapshot
In vivo characterization of diatom multipartite plastid targeting signals
inferredcites →https://doi.org/10.1371/journal.pbio.2006031openalex-snapshot
Provenance and Risk in Transfer of Biological Materials
inferredcites →https://doi.org/10.1371/journal.pbio.2006333openalex-snapshot
Strength in numbers: Collaborative science for new experimental model systems
inferredcites →https://doi.org/10.1371/journal.pone.0003647openalex-snapshot
A One Pot, One Step, Precision Cloning Method with High Throughput Capability
inferredcites →https://doi.org/10.1371/journal.pone.0016765openalex-snapshot
A Modular Cloning System for Standardized Assembly of Multigene Constructs
inferredcites →https://doi.org/10.1371/journal.pone.0021622openalex-snapshot
GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules
inferredcites →https://doi.org/10.1371/journal.pone.0025316openalex-snapshot
A Single Peroxisomal Targeting Signal Mediates Matrix Protein Import in Diatoms
inferredcites →https://doi.org/10.1371/journal.pone.0090372openalex-snapshot
A New and Improved Host-Independent Plasmid System for RK2-Based Conjugal Transfer
inferredcites →https://doi.org/10.1371/journal.pone.0189892openalex-snapshot
Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
inferredcites →https://doi.org/10.1385/1-59259-958-3:107openalex-snapshot
Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
inferredcites →https://doi.org/10.3389/fbioe.2016.00065openalex-snapshot
Refinement of the Diatom Episome Maintenance Sequence and Improvement of Conjugation-Based DNA Delivery Methods
inferredcites →https://doi.org/10.7717/peerj.2344openalex-snapshot
Rapid induction of GFP expression by the nitrate reductase promoter in the diatomPhaeodactylum tricornutum

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1007/s10811-022-02795-yopenalex-snapshot
Phosphate-inducible poly-hydroxy butyrate production dynamics in CO2 supplemented upscaled cultivation of engineered Phaeodactylum tricornutum
inferred← citeshttps://doi.org/10.1016/j.algal.2020.102159openalex-snapshot
Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum
inferred← citeshttps://doi.org/10.1016/j.copbio.2022.102857openalex-snapshot
Plant synthetic biology: from inspiration to augmentation
inferred← citeshttps://doi.org/10.1021/acssynbio.5c00231openalex-snapshot
Real-Time Tracking of Intracellular Prenyl Phosphate Pools in the Marine Diatom Phaeodactylum tricornutum with a Metabolite Protein-Based Biosensor
inferred← citeshttps://doi.org/10.1021/acssynbio.9b00455openalex-snapshot
Extrachromosomal Genetic Engineering of the Marine Diatom Phaeodactylum tricornutum Enables the Heterologous Production of Monoterpenoids
inferred← citeshttps://doi.org/10.1093/nar/gkaa727openalex-snapshot
Rapid, robust plasmid verification by de novo assembly of short sequencing reads
inferred← citeshttps://doi.org/10.1093/synbio/ysab003openalex-snapshot
Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology
inferred← citeshttps://doi.org/10.3389/fbioe.2020.00513openalex-snapshot
Metabolic Engineering Strategies in Diatoms Reveal Unique Phenotypes and Genetic Configurations With Implications for Algal Genetics and Synthetic Biology
inferred← citeshttps://doi.org/10.3389/fbioe.2021.727584openalex-snapshot
Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts
inferred← citeshttps://doi.org/10.3389/fpls.2020.00279openalex-snapshot
Emerging Technologies in Algal Biotechnology: Toward the Establishment of a Sustainable, Algae-Based Bioeconomy
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