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The State of RT-Quantitative PCR: Firsthand Observations of Implementation of Minimum Information for the Publication of Quantitative Real-Time PCR Experiments (MIQE)

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2 of the 24 references listed on this page are in the Retraction Watch database as retracted (export 2026-10-28): 10.1016/s0140-6736(97)11096-0 (notice 2010-02-06), 10.1126/science.1117768 (notice 2007-04-20).

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1007/s12033-012-9539-2openalex-snapshot
Quantitative PCR Pitfalls: The Case of the Human Placenta
inferredcites →https://doi.org/10.1016/j.jmoldx.2012.05.002openalex-snapshot
A New Technology for Stabilization of Biomolecules in Tissues for Combined Histological and Molecular Analyses
inferredcites →https://doi.org/10.1016/j.placenta.2013.03.011openalex-snapshot
RNA degradation differentially affects quantitative mRNA measurements of endogenous reference genes in human placenta
inferredcites →https://doi.org/10.1016/j.ymeth.2009.12.006openalex-snapshot
Why the need for qPCR publication guidelines?—The case for MIQE
inferredcites →https://doi.org/10.1016/j.ymeth.2010.01.005openalex-snapshot
A practical approach to RT-qPCR—Publishing data that conform to the MIQE guidelines
inferredcites →https://doi.org/10.1016/j.ymeth.2012.07.024openalex-snapshot
Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials
inferredcites →https://doi.org/10.1016/s0140-6736(97)11096-0openalex-snapshot
RETRACTED: Ileal-lymphoid-nodular hyperplasia, non-specific colitis, and pervasive developmental disorder in children
inferredcites →https://doi.org/10.1016/s0165-022x(00)00129-9openalex-snapshot
Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR
inferredcites →https://doi.org/10.1016/s0168-1656(99)00163-7openalex-snapshot
Housekeeping genes as internal standards: use and limits
inferredcites →https://doi.org/10.1093/nar/gkr065openalex-snapshot
Measurable impact of RNA quality on gene expression results from quantitative PCR
inferredcites →https://doi.org/10.1093/nar/gkr1013openalex-snapshot
PrimerBank: a PCR primer database for quantitative gene expression analysis, 2012 update
inferredcites →https://doi.org/10.1097/pdm.0b013e3181857e92openalex-snapshot
Impact of Thawing on RNA Integrity and Gene Expression Analysis in Fresh Frozen Tissue
inferredcites →https://doi.org/10.1111/j.1556-4029.2009.01245.xopenalex-snapshot
A Study of PCR Inhibition Mechanisms Using Real Time PCR* , †
inferredcites →https://doi.org/10.1126/science.1117768openalex-snapshot
The mRNA of the Arabidopsis Gene FT Moves from Leaf to Shoot Apex and Induces Flowering
inferredcites →https://doi.org/10.1126/science.316.5823.367copenalex-snapshot
A Thank You from Tulane University
inferredcites →https://doi.org/10.1152/physiolgenomics.00025.2005openalex-snapshot
GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues
inferredcites →https://doi.org/10.1186/1471-2199-14-13openalex-snapshot
How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population
inferredcites →https://doi.org/10.1186/1471-2199-9-62openalex-snapshot
Housekeeping while brain's storming Validation of normalizing factors for gene expression studies in a murine model of traumatic brain injury
inferredcites →https://doi.org/10.1186/1756-0500-2-112openalex-snapshot
Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays
inferredcites →https://doi.org/10.1186/1756-0500-5-222openalex-snapshot
Bone to pick: the importance of evaluating reference genes for RT-qPCR quantification of gene expression in craniosynostosis and bone-related tissues and cells
inferredcites →https://doi.org/10.1371/journal.pone.0059180openalex-snapshot
Careful Selection of Reference Genes Is Required for Reliable Performance of RT-qPCR in Human Normal and Cancer Cell Lines
inferredcites →https://doi.org/10.1373/clinchem.2008.112797openalex-snapshot
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
inferredcites →https://doi.org/10.1373/clinchem.2011.162958openalex-snapshot
Primer Sequence Disclosure: A Clarification of the MIQE Guidelines
inferredcites →https://doi.org/10.3389/fgene.2013.00056openalex-snapshot
Cell-free microRNAs: potential biomarkers in need of standardized reporting

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inferred← citeshttps://doi.org/10.7287/peerj.5432v0.1/reviews/3openalex-snapshot
Peer Review #3 of "Th2 cytokine bias induced by silver nanoparticles in peripheral blood mononuclear cells of common bottlenose dolphins (Tursiops truncatus) (v0.1)"
inferred← citeshttps://doi.org/10.7717/peerj.5432openalex-snapshot
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Leukocyte Migration and Parturition
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