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Target Region Selection Is a Critical Determinant of Community Fingerprints Generated by 16S Pyrosequencing

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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A Mathematical Theory of Communication
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A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria
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Novel Short-Fragment PCR Assay for Highly Sensitive Broad-Spectrum Detection of Anogenital Human Papillomaviruses
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Sequence microdiversity at the ribosomal RNA operons of Escherichia coli pyelonephritogenic strains
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Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns
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Bacterial diversity within the human subgingival crevice
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FastTree: Computing Large Minimum Evolution Trees with Profiles instead of a Distance Matrix
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Comparison of two next-generation sequencing technologies for resolving highly complex microbiota composition using tandem variable 16S rRNA gene regions
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Patterns of sequence variation in two regions of the 16S rRNA multigene family of Escherichia coli
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Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets
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Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates
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Molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis
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Comparison of Species Richness Estimates Obtained Using Nearly Complete Fragments and Simulated Pyrosequencing-Generated Fragments in 16S rRNA Gene-Based Environmental Surveys
inferredcites →https://doi.org/10.1128/aem.03006-05openalex-snapshot
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB
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Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
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Bias in Template-to-Product Ratios in Multitemplate PCR
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Kinetic Bias in Estimates of Coastal Picoplankton Community Structure Obtained by Measurements of Small-Subunit rRNA Gene PCR Amplicon Length Heterogeneity
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Molecular Characterization of Subject-Specific Oral Microflora during Initial Colonization of Enamel
inferredcites →https://doi.org/10.1128/cmr.17.4.840-862.2004openalex-snapshot
Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases
inferredcites →https://doi.org/10.1128/jb.183.12.3770-3783.2001openalex-snapshot
Bacterial Diversity in Human Subgingival Plaque
inferredcites →https://doi.org/10.1128/jcm.00317-06openalex-snapshot
Changes in Periodontal Health Status Are Associated with Bacterial Community Shifts as Assessed by Quantitative 16S Cloning and Sequencing
inferredcites →https://doi.org/10.1128/jcm.01821-09openalex-snapshot
Response of Subgingival Bacteria to Smoking Cessation
inferredcites →https://doi.org/10.1128/jcm.43.11.5721-5732.2005openalex-snapshot
Defining the Normal Bacterial Flora of the Oral Cavity
inferredcites →https://doi.org/10.1128/jcm.43.8.3944-3955.2005openalex-snapshot
Identification of Candidate Periodontal Pathogens and Beneficial Species by Quantitative 16S Clonal Analysis
inferredcites →https://doi.org/10.1177/0022034510370026openalex-snapshot
Analyzing Endodontic Infections by Deep Coverage Pyrosequencing
inferredcites →https://doi.org/10.1177/0022034510377203openalex-snapshot
Subgingival Microbial Profiles of Smokers with Periodontitis
inferredcites →https://doi.org/10.1177/154405910808701104openalex-snapshot
Pyrosequencing analysis of the Oral Microflora of healthy adults
inferredcites →https://doi.org/10.1186/1471-2105-12-38openalex-snapshot
Removing Noise From Pyrosequenced Amplicons
inferredcites →https://doi.org/10.1186/1471-2180-9-259openalex-snapshot
Defining the healthy "core microbiome" of oral microbial communities
inferredcites →https://doi.org/10.1186/gb-2007-8-7-r143openalex-snapshot
Accuracy and quality of massively parallel DNA pyrosequencing
inferredcites →https://doi.org/10.1371/journal.pone.0007401openalex-snapshot
Conservative Fragments in Bacterial 16S rRNA Genes and Primer Design for 16S Ribosomal DNA Amplicons in Metagenomic Studies
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Periodontal Status in Smokers and Never‐Smokers: Clinical Findings and Real‐Time Polymerase Chain Reaction Quantification of Putative Periodontal Pathogens
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Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome
inferredcites →https://doi.org/10.7275/4vng-5608openalex-snapshot
Notes on the use of data transformations

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Parents with periodontitis impact the subgingival colonization of their offspring
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The oral microbiome
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Microbiome depiction through user-adapted bioinformatic pipelines and parameters
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Stress Response of the Juvenile Tri‐Spine Horseshoe Crab Tachypleus tridentatus to Hypoxia: Insights from Phenotypic, Metabolomic, and Microbial Analyses
inferred← citeshttps://doi.org/10.1111/1755-0998.13097openalex-snapshot
Exploring the accuracy of amplicon‐based internal transcribed spacer markers for a fungal community
inferred← citeshttps://doi.org/10.1111/apm.12015openalex-snapshot
Methanogenic archaea in subgingival sites: a review
inferred← citeshttps://doi.org/10.1111/clr.14361openalex-snapshot
Impact of Glycine Powder Air‐Abrasive Debridement on Peri‐Implant Mucositis: A Randomized Control Trial of Clinical, Microbial, and Immunological Changes
inferred← citeshttps://doi.org/10.1111/iej.13389openalex-snapshot
Microbial ecology and predicted metabolic pathways in various oral environments from patients with acute endodontic infections
inferred← citeshttps://doi.org/10.1111/iej.13656openalex-snapshot
A critical analysis of research methods and experimental models to study the root canal microbiome
inferred← citeshttps://doi.org/10.1111/j.1574-6968.2011.02434.xopenalex-snapshot
Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons
inferred← citeshttps://doi.org/10.1111/j.1600-051x.2012.01856.xopenalex-snapshot
Pyrosequencing reveals unique microbial signatures associated with healthy and failing dental implants
inferred← citeshttps://doi.org/10.1111/j.1600-0765.2012.01485.xopenalex-snapshot
Levels of Selenomonas species in generalized aggressive periodontitis
inferred← citeshttps://doi.org/10.1111/j.2041-1014.2012.00642.xopenalex-snapshot
Using high throughput sequencing to explore the biodiversity in oral bacterial communities
inferred← citeshttps://doi.org/10.1111/jcpe.12862openalex-snapshot
Shift in the subgingival microbiome following scaling and root planing in generalized aggressive periodontitis
inferred← citeshttps://doi.org/10.1111/jcpe.13815openalex-snapshot
Parents with periodontitis drive the early acquisition of dysbiotic microbiomes in their offspring
inferred← citeshttps://doi.org/10.1111/jcpe.70063openalex-snapshot
Dysbiosis‐Mediated Inflammation: A Pathophysiological Link Between Rheumatoid Arthritis and Periodontitis
inferred← citeshttps://doi.org/10.1111/jopr.13270openalex-snapshot
Microbiological Profile and Human Immune Response Associated with Peri‐Implantitis: A Systematic Review
inferred← citeshttps://doi.org/10.1111/jopr.13743openalex-snapshot
Microbiological profile of peri‐implantitis: Analyses of peri‐implant microbiome
inferred← citeshttps://doi.org/10.1111/jre.12597openalex-snapshot
Smoking habit modulates peri‐implant microbiome: A case‐control study
inferred← citeshttps://doi.org/10.1111/mec.12611openalex-snapshot
Stability and phylogenetic correlation in gut microbiota: lessons from ants and apes
inferred← citeshttps://doi.org/10.1111/odi.12657openalex-snapshot
Dysbiosis of oral buccal mucosa microbiota in patients with oral lichen planus
inferred← citeshttps://doi.org/10.1111/prd.12010openalex-snapshot
Lessons learned and unlearned in periodontal microbiology
inferred← citeshttps://doi.org/10.1111/prd.12358openalex-snapshot
Did Omics change periodontal therapy?
inferred← citeshttps://doi.org/10.1111/prd.12359openalex-snapshot
Optimizing the quality of clinical studies on oral microbiome: A practical guide for planning, performing, and reporting
inferred← citeshttps://doi.org/10.1128/aem.00513-14openalex-snapshot
Diversity and Transport of Microorganisms in Intertidal Sands of the California Coast
inferred← citeshttps://doi.org/10.1128/aem.01826-15openalex-snapshot
Topographical Mapping of the Rainbow Trout (Oncorhynchus mykiss) Microbiome Reveals a Diverse Bacterial Community with Antifungal Properties in the Skin
inferred← citeshttps://doi.org/10.1128/aem.02414-13openalex-snapshot
Analysis of the Gull Fecal Microbial Community Reveals the Dominance of Catellicoccus marimammalium in Relation to Culturable Enterococci
inferred← citeshttps://doi.org/10.1128/aem.03534-14openalex-snapshot
High-Resolution Taxonomic Profiling of the Subgingival Microbiome for Biomarker Discovery and Periodontitis Diagnosis
inferred← citeshttps://doi.org/10.1128/jcm.01678-14openalex-snapshot
Single-Molecule Long-Read 16S Sequencing To Characterize the Lung Microbiome from Mechanically Ventilated Patients with Suspected Pneumonia
inferred← citeshttps://doi.org/10.1128/mra.01230-24openalex-snapshot
Complete genome sequence of Burkholderia contaminans PCR-C_Y9 isolated from Pericarpium citri reticulatae 'Chachiensis'
inferred← citeshttps://doi.org/10.1128/msystems.00006-17openalex-snapshot
Moderate Exercise Has Limited but Distinguishable Effects on the Mouse Microbiome
inferred← citeshttps://doi.org/10.1128/msystems.00127-16openalex-snapshot
Microbiome Helper: a Custom and Streamlined Workflow for Microbiome Research
inferred← citeshttps://doi.org/10.1128/msystems.00518-21openalex-snapshot
Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
inferred← citeshttps://doi.org/10.1128/spectrum.04327-22openalex-snapshot
Characteristics of the Salivary Microbiota in Periodontal Diseases and Potential Roles of Individual Bacterial Species To Predict the Severity of Periodontal Disease
inferred← citeshttps://doi.org/10.1139/cjm-2014-0503openalex-snapshot
Pyrosequencing analysis of free-living and attached bacterial communities in Meiliang Bay, Lake Taihu, a large eutrophic shallow lake in China
inferred← citeshttps://doi.org/10.1139/cjm-2017-0100openalex-snapshot
Degenerate ITS7 primer enhances oomycete community coverage and PCR sensitivity to Aphanomyces species, economically important plant pathogens
inferred← citeshttps://doi.org/10.1155/2013/958719openalex-snapshot
Microbial Diversity in the Era of Omic Technologies
inferred← citeshttps://doi.org/10.1155/2015/480386openalex-snapshot
Perspective for Aquaponic Systems: “ Omic ” Technologies for Microbial Community Analysis
inferred← citeshttps://doi.org/10.1155/2021/8876926openalex-snapshot
Isolation and Characterization of Gram-Negative Bacterial Species from Pasteurized Dairy Products: Potential Risk to Consumer Health
inferred← citeshttps://doi.org/10.11606/t.42.2018.tde-26032018-164037openalex-snapshot
Desenvolvimento e validação de método para a identificação de micro-organismos metabolicamente ativos em biofilmes de amostras ambientais através da análise de rRNA 16S.
inferred← citeshttps://doi.org/10.1177/0022034513504950openalex-snapshot
Patient-specific Analysis of Periodontal and Peri-implant Microbiomes
inferred← citeshttps://doi.org/10.1177/0022034515590581openalex-snapshot
The Influence of Smoking on the Peri-Implant Microbiome
inferred← citeshttps://doi.org/10.1177/0022034520906842openalex-snapshot
Subgingival Host-Microbial Interactions in Hyperglycemic Individuals
inferred← citeshttps://doi.org/10.1177/00220345211015948openalex-snapshot
Sources of SARS-CoV-2 and Other Microorganisms in Dental Aerosols
inferred← citeshttps://doi.org/10.1186/0717-6287-47-67openalex-snapshot
Acidobacteriaappear to dominate the microbiome of two sympatric Caribbean Sponges and one Zoanthid
inferred← citeshttps://doi.org/10.1186/1471-2105-14-79openalex-snapshot
Comparative analysis of microbiome measurement platforms using latent variable structural equation modeling
inferred← citeshttps://doi.org/10.1186/2049-2618-2-43openalex-snapshot
Characterization and comparison of bacterial communities in benign vocal fold lesions
inferred← citeshttps://doi.org/10.1186/s12866-017-0927-4openalex-snapshot
Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform
inferred← citeshttps://doi.org/10.1186/s12898-016-0068-xopenalex-snapshot
A metabarcoding framework for facilitated survey of endolithic phototrophs with tufA
inferred← citeshttps://doi.org/10.1186/s12903-023-02917-zopenalex-snapshot
Pre-treatment oral microbiome analysis and salivary Stephan curve kinetics in white spot lesion development in orthodontic patients wearing fixed appliances. A pilot study
inferred← citeshttps://doi.org/10.1186/s13071-017-2579-5openalex-snapshot
Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia
inferred← citeshttps://doi.org/10.1186/s13099-025-00750-zopenalex-snapshot
Gut microbiome-based machine learning model for early colorectal cancer and adenoma screening
inferred← citeshttps://doi.org/10.1186/s40168-017-0248-8openalex-snapshot
Intestinal dysbiosis in preterm infants preceding necrotizing enterocolitis: a systematic review and meta-analysis
inferred← citeshttps://doi.org/10.1186/s40168-018-0443-2openalex-snapshot
Characterizing oral microbial communities across dentition states and colonization niches
inferred← citeshttps://doi.org/10.1186/s40168-020-00841-wopenalex-snapshot
Construction of habitat-specific training sets to achieve species-level assignment in 16S rRNA gene datasets
inferred← citeshttps://doi.org/10.1186/s40168-021-01059-0openalex-snapshot
Identifying biases and their potential solutions in human microbiome studies
inferred← citeshttps://doi.org/10.1186/s42523-023-00239-7openalex-snapshot
The impact of culture systems on the gut microbiota and gut metabolome of bighead carp (Hypophthalmichthys nobilis)
inferred← citeshttps://doi.org/10.1201/9780429276170-7openalex-snapshot
Molecular Techniques for Detection of Foodborne Pathogens: Salmonella and Bacillus cereus
inferred← citeshttps://doi.org/10.1201/b18618-4openalex-snapshot
Taxonomic Precision of Different Hypervariable Regions of 16S rRNA Gene and Annotation Methods for Functional Bacterial Groups in Biological Wastewater Treatment
inferred← citeshttps://doi.org/10.12677/qrb.2014.12007openalex-snapshot
The Application of 16SrRNA Sequences Analysis in Oral Microbial Community
inferred← citeshttps://doi.org/10.1371/journal.pcbi.1002863openalex-snapshot
A Guide to Enterotypes across the Human Body: Meta-Analysis of Microbial Community Structures in Human Microbiome Datasets
inferred← citeshttps://doi.org/10.1371/journal.pone.0034233openalex-snapshot
Increased Gut Permeability and Microbiota Change Associate with Mesenteric Fat Inflammation and Metabolic Dysfunction in Diet-Induced Obese Mice
inferred← citeshttps://doi.org/10.1371/journal.pone.0044105openalex-snapshot
Exploring Bacterial Diversity in Hospital Environments by GS-FLX Titanium Pyrosequencing
inferred← citeshttps://doi.org/10.1371/journal.pone.0053649openalex-snapshot
Biased Diversity Metrics Revealed by Bacterial 16S Pyrotags Derived from Different Primer Sets
inferred← citeshttps://doi.org/10.1371/journal.pone.0057782openalex-snapshot
Detection of Transient Bacteraemia following Dental Extractions by 16S rDNA Pyrosequencing: A Pilot Study
inferred← citeshttps://doi.org/10.1371/journal.pone.0061516openalex-snapshot
Investigation of the Effect of Type 2 Diabetes Mellitus on Subgingival Plaque Microbiota by High-Throughput 16S rDNA Pyrosequencing
inferred← citeshttps://doi.org/10.1371/journal.pone.0066934openalex-snapshot
Characterization of the Gut-Associated Microbiome in Inflammatory Pouch Complications Following Ileal Pouch-Anal Anastomosis
inferred← citeshttps://doi.org/10.1371/journal.pone.0071360openalex-snapshot
The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies
inferred← citeshttps://doi.org/10.1371/journal.pone.0076185openalex-snapshot
Taxonomic Precision of Different Hypervariable Regions of 16S rRNA Gene and Annotation Methods for Functional Bacterial Groups in Biological Wastewater Treatment
inferred← citeshttps://doi.org/10.1371/journal.pone.0077599openalex-snapshot
An In Vitro Model of the Horse Gut Microbiome Enables Identification of Lactate-Utilizing Bacteria That Differentially Respond to Starch Induction
inferred← citeshttps://doi.org/10.1371/journal.pone.0084438openalex-snapshot
Archaea Appear to Dominate the Microbiome of Inflatella pellicula Deep Sea Sponges
inferred← citeshttps://doi.org/10.1371/journal.pone.0095567openalex-snapshot
Systematic Design of 18S rRNA Gene Primers for Determining Eukaryotic Diversity in Microbial Consortia
inferred← citeshttps://doi.org/10.1371/journal.pone.0099722openalex-snapshot
The Bias Associated with Amplicon Sequencing Does Not Affect the Quantitative Assessment of Bacterial Community Dynamics
inferred← citeshttps://doi.org/10.1371/journal.pone.0140725openalex-snapshot
Characterization of Bacterial Communities Associated with the Tyrian Purple Producing Gland in a Marine Gastropod
inferred← citeshttps://doi.org/10.1371/journal.pone.0148047openalex-snapshot
Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples
inferred← citeshttps://doi.org/10.1371/journal.pone.0184223openalex-snapshot
Changes in soil microbial communities after 10 years of winter wheat cultivation versus fallow in an organic-poor soil in the Loess Plateau of China
inferred← citeshttps://doi.org/10.1371/journal.pone.0186161openalex-snapshot
Modified RNA-seq method for microbial community and diversity analysis using rRNA in different types of environmental samples
inferred← citeshttps://doi.org/10.1371/journal.pone.0209887openalex-snapshot
Seabird and pinniped shape soil bacterial communities of their settlements in Cape Shirreff, Antarctica
inferred← citeshttps://doi.org/10.1371/journal.pone.0331145openalex-snapshot
FAVABEAN and FALAPhyl: Open-source pipelines for scalable 16s rRNA microbiome data processing and visualization
inferred← citeshttps://doi.org/10.14814/phy2.15254openalex-snapshot
Obesity influences composition of salivary and fecal microbiota and impacts the interactions between bacterial taxa
inferred← citeshttps://doi.org/10.1590/0001-3765202120190183openalex-snapshot
Structure and diversity of bacterial community in semiarid soils cultivated with prickly-pear cactus (Opuntia ficus-indica (L.) Mill.)
inferred← citeshttps://doi.org/10.1603/en12260openalex-snapshot
Low-Level Detection ofCandidatusLiberibacter Solanacearum inBactericera cockerelli(Hemiptera: Triozidae) by 16s rRna Pyrosequencing: Table 1.
inferred← citeshttps://doi.org/10.17957/ijab/15.0511openalex-snapshot
Bacillus subtilis N3 as a Biocontrol Agent for Curvularia lunata and its Antifungal Protein Properties
inferred← citeshttps://doi.org/10.18174/274416openalex-snapshot
Community and genomic analysis of the human small intestine microbiota
inferred← citeshttps://doi.org/10.1902/jop.2017.160542openalex-snapshot
Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome
inferred← citeshttps://doi.org/10.2106/jbjs.22.00315openalex-snapshot
Application of Nucleic Acid-Based Strategies to Detect Infectious Pathogens in Orthopaedic Implant-Related Infection
inferred← citeshttps://doi.org/10.21203/rs.3.rs-100088/v1openalex-snapshot
16s rRNA Lung Microbiota Study in Mechanically Ventilated Patient: A Pilot Study
inferred← citeshttps://doi.org/10.21203/rs.3.rs-4474939/v1openalex-snapshot
Unique region of 16S rRNA gene of Mycobacterium tuberculosis complex sequences isolated from pulmonary tuberculosis patients in Surabaya, Indonesia
inferred← citeshttps://doi.org/10.22175/mmb.20022openalex-snapshot
Gut Microbiota and Advances in Microbiome Sequencing-Based Technologies: Opportunities for Potential Biologics Discovery in Meat Animals
inferred← citeshttps://doi.org/10.26481/dis.20180301dtopenalex-snapshot
Microbiota profiling in gastrointestinal health and disease
inferred← citeshttps://doi.org/10.31390/gradschool_theses.2877openalex-snapshot
The Distribution and Diversity of Functional Gene Pathways Controlling Sulfur Speciation in Lower Kane Cave, Wyoming
inferred← citeshttps://doi.org/10.31390/gradschool_theses.4269openalex-snapshot
Longitudinal Assessment of Shifting Microbial Community Composition in Sandy Beaches Following the 2010 Deepwater Horizon Oil Spill and Remediation Efforts
inferred← citeshttps://doi.org/10.3354/ame01758openalex-snapshot
River bacterioplankton community responses to a high inflow event
inferred← citeshttps://doi.org/10.3354/dao03432openalex-snapshot
Technical pitfalls that bias comparative microbial community analyses of aquatic disease Ian Hewson*
inferred← citeshttps://doi.org/10.3382/ps.2014-03974openalex-snapshot
Spatial heterogeneity and stability of bacterial community in the gastrointestinal tracts of broiler chickens
inferred← citeshttps://doi.org/10.3389/fmicb.2013.00189openalex-snapshot
Microbial activity in the marine deep biosphere: progress and prospects
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