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Highly Precise Measurement of HIV DNA by Droplet Digital PCR

https://doi.org/10.1371/journal.pone.0055943
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Outgoing claims: the papers this work cites, builds on, or argues with.

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The Amino Terminal Domain of HIV-1 Rev is Required for Discrimination of the RRE from Nonspecific RNA
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Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy
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Rational design of HIV-1 fluorescent hydrolysis probes considering phylogenetic variation and probe performance
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Digital PCR provides absolute quantitation of viral load for an occult RNA virus
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Evaluation of Digital PCR for Absolute DNA Quantification
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Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR
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High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
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Rapid turnover of plasma virions and CD4 lymphocytes in HIV-1 infection
inferredcites →https://doi.org/10.1038/87979openalex-snapshot
A quantitative assay for HIV DNA integration in vivo
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Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
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Decreased expression of AMPA receptor messenger RNA and protein in AIDS: A model for HIV-associated neurotoxicity
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Heterogeneous clearance rates of long-lived lymphocytes infected with HIV: Intrinsic stability predicts lifelong persistence
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Digital PCR
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Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
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Probing Individual Environmental Bacteria for Viruses by Using Microfluidic Digital PCR
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High Levels of HIV-1 in Plasma During All Stages of Infection Determined By Competitive PCR
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Single-Molecule Detection of Epidermal Growth Factor Receptor Mutations in Plasma by Microfluidics Digital PCR in Non–Small Cell Lung Cancer Patients
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Digital PCR provides sensitive and absolute calibration for high throughput sequencing
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Partly Nonparametric Approach for Determining the Limit of Detection
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Microfluidics Digital PCR Reveals a Higher than Expected Fraction of Fetal DNA in Maternal Plasma
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Principle and applications of digital PCR
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HIV-1 Messenger RNA in Peripheral Blood Mononuclear Cells as an Early Marker of Risk for Progression to AIDS

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Development and optimization of diagnostic assays for infectious diseases
inferred← citeshttps://doi.org/10.1016/j.lwt.2021.111388openalex-snapshot
Rapid and absolute quantification of VBNC Cronobacter sakazakii by PMAxx combined with single intact cell droplet digital PCR in infant foods
inferred← citeshttps://doi.org/10.1016/j.meegid.2014.03.028openalex-snapshot
Comparison of a quantitative Real-Time PCR assay and droplet digital PCR for copy number analysis of the CCL4L genes
inferred← citeshttps://doi.org/10.1016/j.mimet.2016.03.012openalex-snapshot
Optimisation of a droplet digital PCR assay for the diagnosis of Schistosoma japonicum infection: A duplex approach with DNA binding dye chemistry
inferred← citeshttps://doi.org/10.1016/j.mimet.2018.07.005openalex-snapshot
Quantification of plasmid DNA standards for U.S. EPA fecal indicator bacteria qPCR methods by droplet digital PCR analysis
inferred← citeshttps://doi.org/10.1016/j.molbiopara.2018.04.004openalex-snapshot
Circulating cell-free DNA in patients with alveolar echinococcosis
inferred← citeshttps://doi.org/10.1016/j.molmet.2019.06.013openalex-snapshot
β cell responses to inflammation
inferred← citeshttps://doi.org/10.1016/j.omtm.2020.04.016openalex-snapshot
Vector Copy Distribution at a Single-Cell Level Enhances Analytical Characterization of Gene-Modified Cell Therapies
inferred← citeshttps://doi.org/10.1016/j.omtm.2021.05.003openalex-snapshot
Development of a one-step RT-ddPCR method to determine the expression and potency of AAV vectors
inferred← citeshttps://doi.org/10.1016/j.patbio.2014.07.007openalex-snapshot
Quantification of viral DNA during HIV-1 infection: A review of relevant clinical uses and laboratory methods
inferred← citeshttps://doi.org/10.1016/j.snb.2013.12.089openalex-snapshot
Interfacing picoliter droplet microfluidics with addressable microliter compartments using fluorescence activated cell sorting
inferred← citeshttps://doi.org/10.1016/j.snb.2021.130678openalex-snapshot
A low-cost, programmable, and multi-functional droplet printing system for low copy number SARS-CoV-2 digital PCR determination
inferred← citeshttps://doi.org/10.1016/j.stem.2024.03.002openalex-snapshot
A simultaneous knockout knockin genome editing strategy in HSPCs potently inhibits CCR5- and CXCR4-tropic HIV-1 infection
inferred← citeshttps://doi.org/10.1016/j.talanta.2020.121726openalex-snapshot
Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
inferred← citeshttps://doi.org/10.1016/j.talanta.2021.122227openalex-snapshot
Quantitative analysis of RNA by HPLC and evaluation of RT-dPCR for coronavirus RNA quantification
inferred← citeshttps://doi.org/10.1016/j.theriogenology.2019.01.037openalex-snapshot
Insight into bovine (Bos indicus) spermatozoal whole transcriptome profile
inferred← citeshttps://doi.org/10.1016/j.tim.2015.01.013openalex-snapshot
Towards an HIV-1 cure: measuring the latent reservoir
inferred← citeshttps://doi.org/10.1016/j.tube.2016.04.004openalex-snapshot
Digital PCR assay detection of circulating Mycobacterium tuberculosis DNA in pulmonary tuberculosis patient plasma
inferred← citeshttps://doi.org/10.1016/j.tube.2019.07.001openalex-snapshot
Droplet digital PCR applications in the tuberculosis world
inferred← citeshttps://doi.org/10.1016/j.virol.2017.05.008openalex-snapshot
Relative efficacy of T cell stimuli as inducers of productive HIV-1 replication in latently infected CD4 lymphocytes from patients on suppressive cART
inferred← citeshttps://doi.org/10.1016/j.virol.2017.08.005openalex-snapshot
Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses
inferred← citeshttps://doi.org/10.1016/j.watres.2019.115213openalex-snapshot
Droplet digital PCR quantification of norovirus and adenovirus in decentralized wastewater and graywater collections: Implications for onsite reuse
inferred← citeshttps://doi.org/10.1016/j.watres.2020.116085openalex-snapshot
A novel droplet digital PCR human mtDNA assay for fecal source tracking
inferred← citeshttps://doi.org/10.1016/j.xcrm.2021.100243openalex-snapshot
A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir
inferred← citeshttps://doi.org/10.1016/j.ymeth.2019.05.013openalex-snapshot
Multiplexed primer extension sequencing: A targeted RNA-seq method that enables high-precision quantitation of mRNA splicing isoforms and rare pre-mRNA splicing intermediates
inferred← citeshttps://doi.org/10.1016/j.ymeth.2021.03.006openalex-snapshot
An assessment of the reproducibility of reverse transcription digital PCR quantification of HIV-1
inferred← citeshttps://doi.org/10.1016/j.ymeth.2021.04.008openalex-snapshot
In pursuit of sensitivity: Lessons learned from viral nucleic acid detection and quantification on the Raindance ddPCR platform
inferred← citeshttps://doi.org/10.1016/j.ymeth.2021.04.011openalex-snapshot
Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts
inferred← citeshttps://doi.org/10.1016/j.ymeth.2021.04.025openalex-snapshot
Ultrasensitive detection and quantification of viral nucleic acids with Raindance droplet digital PCR (ddPCR)
inferred← citeshttps://doi.org/10.1016/s1872-2040(16)60918-0openalex-snapshot
Research and Application Progress of Digital Nucleic Acid Amplification Detection Techniques
inferred← citeshttps://doi.org/10.1016/s2055-6640(20)30460-xopenalex-snapshot
Diagnostic utility of droplet digital PCR for HIV reservoir quantification
inferred← citeshttps://doi.org/10.1017/s0031182016001864openalex-snapshot
Validation of droplet digital PCR for the detection and absolute quantification of Borrelia DNA in Ixodes scapularis ticks
inferred← citeshttps://doi.org/10.1021/ac403061nopenalex-snapshot
Multiplexed Target Detection Using DNA-Binding Dye Chemistry in Droplet Digital PCR
inferred← citeshttps://doi.org/10.1021/ac501118vopenalex-snapshot
Accurate Determination of Plasmid Copy Number of Flow-Sorted Cells using Droplet Digital PCR
inferred← citeshttps://doi.org/10.1021/ac5041617openalex-snapshot
Highly Reproducible Absolute Quantification of Mycobacterium tuberculosis Complex by Digital PCR
inferred← citeshttps://doi.org/10.1021/acs.analchem.0c02890openalex-snapshot
Validation of Preamplification to Improve Quantification of Cytomegalovirus DNA Using Droplet Digital Polymerase Chain Reaction
inferred← citeshttps://doi.org/10.1021/acs.analchem.1c02414openalex-snapshot
Pipette-Tip-Enabled Digital Nucleic Acid Analyzer for COVID-19 Testing with Isothermal Amplification
inferred← citeshttps://doi.org/10.1021/acs.analchem.5b02429openalex-snapshot
Statistical Models for the Analysis and Design of Digital Polymerase Chain Reaction (dPCR) Experiments
inferred← citeshttps://doi.org/10.1021/acs.analchem.6b03328openalex-snapshot
Absolute Quantification of H5-Subtype Avian Influenza Viruses Using Droplet Digital Loop-Mediated Isothermal Amplification
inferred← citeshttps://doi.org/10.1021/acs.analchem.6b03687openalex-snapshot
Droplet-Based Multivolume Digital Polymerase Chain Reaction by a Surface-Assisted Multifactor Fluid Segmentation Approach
inferred← citeshttps://doi.org/10.1021/acs.analchem.6b04208openalex-snapshot
Model-Based Classification for Digital PCR: Your Umbrella for Rain
inferred← citeshttps://doi.org/10.1021/acs.analchem.6b05032openalex-snapshot
Interlaboratory Reproducibility of Droplet Digital Polymerase Chain Reaction Using a New DNA Reference Material Format
inferred← citeshttps://doi.org/10.1021/acs.analchem.6b05070openalex-snapshot
A Targeted Mass Spectrometry Assay for Detection of HIV Gag Protein Following Induction of Latent Viral Reservoirs
inferred← citeshttps://doi.org/10.1021/acs.est.5b00253openalex-snapshot
Droplet Digital Polymerase Chain Reaction (PCR) Outperforms Real-Time PCR in the Detection of Environmental DNA from an Invasive Fish Species
inferred← citeshttps://doi.org/10.1021/acs.jmedchem.5b01233openalex-snapshot
Gnidimacrin, a Potent Anti-HIV Diterpene, Can Eliminate Latent HIV-1 Ex Vivo by Activation of Protein Kinase C β
inferred← citeshttps://doi.org/10.1021/acsnano.3c01917openalex-snapshot
STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads
inferred← citeshttps://doi.org/10.1021/acsnano.4c14913openalex-snapshot
Deactivated Cas9-Engineered Magnetic Micromotors toward a Point-of-Care Digital Viral RNA Assay
inferred← citeshttps://doi.org/10.1038/gt.2016.2openalex-snapshot
Establishment of two quantitative nested qPCR assays targeting the human EPO transgene
inferred← citeshttps://doi.org/10.1038/nm.4156openalex-snapshot
Defective proviruses rapidly accumulate during acute HIV-1 infection
inferred← citeshttps://doi.org/10.1038/nm.4319openalex-snapshot
HIV persistence in tissue macrophages of humanized myeloid-only mice during antiretroviral therapy
inferred← citeshttps://doi.org/10.1038/nm.4347openalex-snapshot
Novel assay reveals a large, inducible, replication-competent HIV-1 reservoir in resting CD4+ T cells
inferred← citeshttps://doi.org/10.1038/nnano.2014.261openalex-snapshot
Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering
inferred← citeshttps://doi.org/10.1038/nrmicro3352openalex-snapshot
Eradicating HIV-1 infection: seeking to clear a persistent pathogen
inferred← citeshttps://doi.org/10.1038/s12276-025-01453-wopenalex-snapshot
Analytical techniques for nucleic acid and protein detection with single-molecule sensitivity
inferred← citeshttps://doi.org/10.1038/s41467-017-01921-6openalex-snapshot
Silencing of the lncRNA Zeb2-NAT facilitates reprogramming of aged fibroblasts and safeguards stem cell pluripotency
inferred← citeshttps://doi.org/10.1038/s41591-020-1105-zopenalex-snapshot
Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids
inferred← citeshttps://doi.org/10.1038/s41598-017-01221-5openalex-snapshot
Instability of 8E5 calibration standard revealed by digital PCR risks inaccurate quantification of HIV DNA in clinical samples by qPCR
inferred← citeshttps://doi.org/10.1038/s41598-017-17057-yopenalex-snapshot
Peripheral T follicular helper Cells Make a Difference in HIV Reservoir Size between Elite Controllers and Patients on Successful cART
inferred← citeshttps://doi.org/10.1038/s41598-019-48681-5openalex-snapshot
HIV-1 DNA-capture-seq is a useful tool for the comprehensive characterization of HIV-1 provirus
inferred← citeshttps://doi.org/10.1038/s41598-020-75958-xopenalex-snapshot
Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR
inferred← citeshttps://doi.org/10.1038/s41598-020-80715-1openalex-snapshot
Digital droplet PCR accurately quantifies SARS-CoV-2 viral load from crude lysate without nucleic acid purification
inferred← citeshttps://doi.org/10.1038/s41598-021-02535-1openalex-snapshot
A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
inferred← citeshttps://doi.org/10.1038/s41598-021-87824-5openalex-snapshot
Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system
inferred← citeshttps://doi.org/10.1038/s41598-021-94812-2openalex-snapshot
Serum miR371 in testicular germ cell cancer before and after orchiectomy, assessed by digital-droplet PCR in a prospective study
inferred← citeshttps://doi.org/10.1038/s41598-022-13581-8openalex-snapshot
Accuracy of real-time PCR and digital PCR for the monitoring of total HIV DNA under prolonged antiretroviral therapy
inferred← citeshttps://doi.org/10.1038/s41598-025-17392-5openalex-snapshot
Duplex digital PCR assay on microfluidic chamber arrays for total HIV DNA reservoir quantification in persons with HIV
inferred← citeshttps://doi.org/10.1038/s41598-025-85297-4openalex-snapshot
Highly viable gastrointestinal Chlamydia trachomatis in women abstaining from receptive anal intercourse
inferred← citeshttps://doi.org/10.1038/srep13179openalex-snapshot
Methamphetamine Use in HIV-infected Individuals Affects T-cell Function and Viral Outcome during Suppressive Antiretroviral Therapy
inferred← citeshttps://doi.org/10.1038/srep13811openalex-snapshot
Comparison of digital PCR platforms and semi-nested qPCR as a tool to determine the size of the HIV reservoir
inferred← citeshttps://doi.org/10.1038/srep17094openalex-snapshot
Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART
inferred← citeshttps://doi.org/10.1039/c4lc01158aopenalex-snapshot
Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP)
inferred← citeshttps://doi.org/10.1039/c6ay00600kopenalex-snapshot
Digital droplet LAMP as a microfluidic app on standard laboratory devices
inferred← citeshttps://doi.org/10.1039/c6lc01305hopenalex-snapshot
Centrifugal micro-channel array droplet generation for highly parallel digital PCR
inferred← citeshttps://doi.org/10.1039/c6lc01489eopenalex-snapshot
Ultra-high throughput detection (1 million droplets per second) of fluorescent droplets using a cell phone camera and time domain encoded optofluidics
inferred← citeshttps://doi.org/10.1039/c9lc00932aopenalex-snapshot
A microfluidic alternating-pull–push active digitization method for sample-loss-free digital PCR
inferred← citeshttps://doi.org/10.1039/d2an00283copenalex-snapshot
Microfluidic flow cytometry for blood-based biomarker analysis
inferred← citeshttps://doi.org/10.1039/d2an01813fopenalex-snapshot
Isothermal nucleic acid amplification technology in HIV detection
showing the first 200 of 498
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