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Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography
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What this work says
Outgoing claims: the papers this work cites, builds on, or argues with.
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Advances in membranous vesicle and exosome proteomics improving biological understanding and biomarker discovery
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Growth of human tumor cell lines in transferrin-free, low-iron medium
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Formation and role of exosomes in cancer
inferredcites →https://doi.org/10.1007/s00018-017-2595-9openalex-snapshot
Current knowledge on exosome biogenesis and release
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Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals
inferredcites →https://doi.org/10.1007/s10555-013-9441-9openalex-snapshot
Exosomes in cancer development, metastasis, and drug resistance: a comprehensive review
inferredcites →https://doi.org/10.1007/s10616-010-9263-3openalex-snapshot
Albumin and mammalian cell culture: implications for biotechnology applications
inferredcites →https://doi.org/10.1016/0092-8674(80)90540-1openalex-snapshot
Serum-free cell culture: a unifying approach
inferredcites →https://doi.org/10.1016/0168-1656(89)90009-6openalex-snapshot
Nutritional aspects of the growth of animal cells in culture
inferredcites →https://doi.org/10.1016/j.jconrel.2014.12.013openalex-snapshot
Biodistribution and delivery efficiency of unmodified tumor-derived exosomes
inferredcites →https://doi.org/10.1016/j.jim.2008.03.001openalex-snapshot
Increased exosome production from tumour cell cultures using the Integra CELLine Culture System
inferredcites →https://doi.org/10.1016/j.jim.2014.06.007openalex-snapshot
Isolation of biologically-active exosomes from human plasma
inferredcites →https://doi.org/10.1016/j.molmed.2014.03.002openalex-snapshot
Extracellular vesicles: emerging targets for cancer therapy
inferredcites →https://doi.org/10.1016/j.nano.2015.01.003openalex-snapshot
Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
inferredcites →https://doi.org/10.1016/j.nano.2017.03.011openalex-snapshot
Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation
inferredcites →https://doi.org/10.1016/j.tcb.2016.11.003openalex-snapshot
Extracellular Vesicles: Unique Intercellular Delivery Vehicles
inferredcites →https://doi.org/10.1016/j.ymeth.2015.02.019openalex-snapshot
Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes
inferredcites →https://doi.org/10.1016/s0022-1759(99)00122-2openalex-snapshot
Efficient laboratory-scale production of monoclonal antibodies using membrane-based high-density cell culture technology
inferredcites →https://doi.org/10.1021/pr900392bopenalex-snapshot
Different Changes in Protein and Phosphoprotein Levels Result from Serum Starvation of High-Grade Glioma and Adenocarcinoma Cell Lines
inferredcites →https://doi.org/10.1038/aps.2013.166openalex-snapshot
Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro
inferredcites →https://doi.org/10.1038/ncb1596openalex-snapshot
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
inferredcites →https://doi.org/10.1038/nmeth.4185openalex-snapshot
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
inferredcites →https://doi.org/10.1038/nrd3978openalex-snapshot
Extracellular vesicles: biology and emerging therapeutic opportunities
inferredcites →https://doi.org/10.1038/nri2567openalex-snapshot
Membrane vesicles as conveyors of immune responses
inferredcites →https://doi.org/10.1038/s41598-017-15717-7openalex-snapshot
Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies
inferredcites →https://doi.org/10.1038/srep33641openalex-snapshot
Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles’ characteristics compared to precipitating agents
inferredcites →https://doi.org/10.1080/20013078.2017.1329476openalex-snapshot
Exosomes purified from a single cell type have diverse morphology
inferredcites →https://doi.org/10.1080/20013078.2017.1344087openalex-snapshot
Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: a variation study
inferredcites →https://doi.org/10.1080/20013078.2017.1422674openalex-snapshot
Efficient ultrafiltration‐based protocol to deplete extracellular vesicles from fetal bovine serum
inferredcites →https://doi.org/10.1083/jcb.201211138openalex-snapshot
Extracellular vesicles: Exosomes, microvesicles, and friends
inferredcites →https://doi.org/10.1093/nar/gks832openalex-snapshot
Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells
inferredcites →https://doi.org/10.1093/nar/gku347openalex-snapshot
Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions
inferredcites →https://doi.org/10.1152/ajpcell.00091.2011openalex-snapshot
Serum starvation: caveat emptor
inferredcites →https://doi.org/10.1155/2014/179486openalex-snapshot
Exosome in Tumour Microenvironment: Overview of the Crosstalk between Normal and Cancer Cells
inferredcites →https://doi.org/10.1155/2018/8545347openalex-snapshot
Isolation of Extracellular Vesicles: General Methodologies and Latest Trends
inferredcites →https://doi.org/10.1158/0008-5472.can-12-3514openalex-snapshot
Automated Tracking of Nanoparticle-labeled Melanoma Cells Improves the Predictive Power of a Brain Metastasis Model
inferredcites →https://doi.org/10.1158/1078-0432.1010.11.3openalex-snapshot
Fas Ligand–Positive Membranous Vesicles Isolated from Sera of Patients with Oral Cancer Induce Apoptosis of Activated T Lymphocytes
inferredcites →https://doi.org/10.1172/jci81129openalex-snapshot
Extracellular vesicle isolation and characterization: toward clinical application
inferredcites →https://doi.org/10.1186/s13075-017-1228-xopenalex-snapshot
Identification of potential saliva and tear biomarkers in primary Sjögren’s syndrome, utilising the extraction of extracellular vesicles and proteomics analysis
inferredcites →https://doi.org/10.1371/journal.pone.0138849openalex-snapshot
Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells
inferredcites →https://doi.org/10.1371/journal.pone.0145686openalex-snapshot
Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods
inferredcites →https://doi.org/10.21767/2172-0479.100018openalex-snapshot
Direct Isolation of Exosomes from Cell Culture: Simplifying Methods for Exosome Enrichment and Analysis
inferredcites →https://doi.org/10.3402/jev.v3.23430openalex-snapshot
Single‐step isolation of extracellular vesicles by size‐exclusion chromatography
inferredcites →https://doi.org/10.3402/jev.v3.24783openalex-snapshot
Importance of exosome depletion protocols to eliminate functional and RNA‐containing extracellular vesicles from fetal bovine serum
inferredcites →https://doi.org/10.3402/jev.v3.26913openalex-snapshot
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles
inferredcites →https://doi.org/10.3402/jev.v4.26373openalex-snapshot
Extracellular vesicle–depleted fetal bovine and human sera have reduced capacity to support cell growth
inferredcites →https://doi.org/10.3402/jev.v4.27031openalex-snapshot
Optimized exosome isolation protocol for cell culture supernatant and human plasma
inferredcites →https://doi.org/10.3402/jev.v4.29509openalex-snapshot
High‐speed centrifugation induces aggregation of extracellular vesicles
inferredcites →https://doi.org/10.3402/jev.v5.29289openalex-snapshot
Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer
inferredcites →https://doi.org/10.3402/jev.v5.32945openalex-snapshot
Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
inferredcites →https://doi.org/10.3892/ijmm.2015.2194openalex-snapshot
Isolation of extracellular vesicles: Determining the correct approach (Review)
What points at this work
Incoming claims: where this work appears in other papers’ reference lists and arguments.
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Exosome‐associated polysialic acid modulates membrane potentials, membrane thermotropic properties, and raft‐dependent interactions between vesicles
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Preservation of Extracellular Vesicle Integrity and Cargo Using Spiral Microfluidic Chip‐Based Separation
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Therapeutic Potential of Extracellular Vesicles for Sepsis Treatment
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GFP‐tagging of extracellular vesicles for rapid process development
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Implications of exosomes as diagnostic and therapeutic strategies in cancer
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Cell culture‐derived extracellular vesicles: Considerations for reporting cell culturing parameters
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Investigating the consistency of extracellular vesicle production from breast cancer subtypes using CELLine adherent bioreactors
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Workflow for the Isolation and Characterisation of Human Milk Extracellular Vesicles (HMEVs) and Their Inflammatory Biomarker Profile
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Extracellular Vesicles in Head and Neck Cancer—A Systematic Review
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Nerve Growth Factor Treatment and the Corneal Epithelium: Extracellular Vesicle‐Mediated Signaling
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Defining the influence of size‐exclusion chromatography fraction window and ultrafiltration column choice on extracellular vesicle recovery in a skeletal muscle model
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Isolation, characterization, and fibroblast uptake of bacterial extracellular vesicles from Porphyromonas gingivalis strains
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TAF1 Transcripts and Neurofilament Light Chain as Biomarkers for X‐linked Dystonia‐Parkinsonism
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Impact of chemically defined culture media formulations on extracellular vesicle production by amniotic epithelial cells
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Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use
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Solving the Contamination Conundrum Derived from Coisolation of Extracellular Vesicles and Lipoproteins: Approaches for Isolation and Characterization
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Progress in extracellular vesicle biology and their application in cancer medicine
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Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask
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Methods to Separate, Characterize, and Encapsulate Drug Molecules into Exosomes for Targeted Delivery and Treatment of Glioblastoma
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A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
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Characterizing Extracellular Vesicles Using Nanoparticle-Tracking Analysis
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Exosomal MicroRNA Profiling
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Extracellular Vesicles: The Challenges on the Way and Engineering Perspectives
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Updated Methods of Extracellular Vesicles Isolation
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Extracellular Vesicles: The Challenges on the Way and Engineering Perspectives
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Konzept der „liquid biopsy“ in der Versorgung maligner Augentumoren
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Recent Advancement and Technical Challenges in Developing Small Extracellular Vesicles for Cancer Drug Delivery
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Engineering of Exosomes to Target Cancer Metastasis
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The role of extracellular vesicles-associated proteins markers in prostate cancer: a review
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Comparative Analysis of MSC-Derived Exosomes Depending on Cell Culture Media for Regenerative Bioactivity
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Advances in biomarker detection: Alternative approaches for blood-based biomarker detection
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Advances in extracellular vesicles analysis
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Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light-sheet fluorescence microscopy
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Quantification of mtDNA in extracellular vesicles
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miRNA-124 loaded extracellular vesicles encapsulated within hydrogel matrices for combating chemotherapy-induced neurodegeneration
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High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography
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Emerging Frontiers in acute kidney injury: The role of extracellular vesicles
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Emerging technologies and commercial products in exosome-based cancer diagnosis and prognosis
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Microfluidic-based exosome isolation and highly sensitive aptamer exosome membrane protein detection for lung cancer diagnosis
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Bridging laboratory innovation to translational research and commercialization of extracellular vesicle isolation and detection
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Technological aspects of manufacturing and analytical control of biological nanoparticles
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Modern isolation and separation techniques for extracellular vesicles
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Micropatterned growth surface topography affects extracellular vesicle production
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Exosome detection via the ultrafast-isolation system: EXODUS
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Microdomains form on the luminal face of neuronal extracellular vesicle membranes
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Nanoscale sorting of extracellular vesicles via optically-induced dielectrophoresis on an integrated microfluidic system
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Unraveling the complexity of the extracellular vesicle landscape with advanced proteomics
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Comprehensive Review of Transcriptomics (RNAs) Workflows from Blood Specimens
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Metabolic signature of extracellular vesicles depends on the cell culture conditions
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Purity and yield of melanoma exosomes are dependent on isolation method
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Small extracellular vesicles derived from sequential stimulation of canine adipose-derived mesenchymal stem cells enhance anti-inflammatory activity
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Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers
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Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions
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A new paradigm for diagnosis of neurodegenerative diseases: peripheral exosomes of brain origin
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Towards establishing extracellular vesicle-associated RNAs as biomarkers for HER2+ breast cancer
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Towards establishing extracellular vesicle-associated RNAs as biomarkers for HER2+ breast cancer
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Towards establishing extracellular vesicle-associated RNAs as biomarkers for HER2+ breast cancer
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Clinical impact of different exosomes’ protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
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Cancer cell line-specific protein profiles in extracellular vesicles identified by proteomics
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Novel biomarker and drug delivery systems for theranostics – extracellular vesicles
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Production of extracellular vesicles from equine embryo-derived mesenchymal stromal cells
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Nanoparticles from culture media are internalized by in vitro-produced bovine embryos and its depletion affect expression of pluripotency genes
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Detection of Circulating Tumor Cells and Epithelial Progenitor Cells: A Comprehensive Study
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Extracellular vesicles in liquid biopsies: there is hope for oral squamous cell carcinoma
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Fungi extracellular vesicles: extraction, cargo, and the immune system response
inferred← citeshttps://doi.org/10.21203/rs.3.rs-1362793/v1openalex-snapshot
Scale-Up Production Of Extracellular Vesicles From Mesenchymal Stromal Cells Isolated From Pre-Implantation Equine Embryos
inferred← citeshttps://doi.org/10.21203/rs.3.rs-5741549/v1openalex-snapshot
Extracellular Vesicles Enhance Osteogenic Differentiation of Mesenchymal Stem Cells without any Chemical Agents
inferred← citeshttps://doi.org/10.2147/ijn.s239548openalex-snapshot
Exosomes as Actively Targeted Nanocarriers for Cancer Therapy
inferred← citeshttps://doi.org/10.2147/ijn.s463296openalex-snapshot
The Landscape of Exosomes Biogenesis to Clinical Applications
inferred← citeshttps://doi.org/10.2147/ijn.s479697openalex-snapshot
Recent Progress of Exosomes in Hematological Malignancies: Pathogenesis, Diagnosis, and Therapeutic Strategies
inferred← citeshttps://doi.org/10.3389/fbioe.2024.1441552openalex-snapshot
Bioprocessing strategies for enhanced probiotic extracellular vesicle production: culture condition modulation
inferred← citeshttps://doi.org/10.3389/fcell.2024.1382552openalex-snapshot
Effect of 3D and 2D cell culture systems on trophoblast extracellular vesicle physico-chemical characteristics and potency
inferred← citeshttps://doi.org/10.3389/fimmu.2025.1525052openalex-snapshot
Prospect of extracellular vesicles in tumor immunotherapy
inferred← citeshttps://doi.org/10.3389/fmed.2025.1539714openalex-snapshot
The potential of exosomes in regenerative medicine and in the diagnosis and therapies of neurodegenerative diseases and cancer
inferred← citeshttps://doi.org/10.3389/fmolb.2025.1492282openalex-snapshot
The effect of extracellular vesicles derived from oral squamous cell carcinoma on the metabolic profile of oral fibroblasts
inferred← citeshttps://doi.org/10.3389/fonc.2024.1456346openalex-snapshot
Extracellular vesicles from cancer cell lines of different origins drive the phenotype of normal oral fibroblasts in a CAF-like direction
inferred← citeshttps://doi.org/10.3390/biomedicines9081061openalex-snapshot
Exosomes: Potential Disease Biomarkers and New Therapeutic Targets
inferred← citeshttps://doi.org/10.3390/bios13080802openalex-snapshot
Biogenesis, Isolation, and Detection of Exosomes and Their Potential in Therapeutics and Diagnostics
inferred← citeshttps://doi.org/10.3390/cancers12113156openalex-snapshot
Size-Exclusion Chromatography as a Technique for the Investigation of Novel Extracellular Vesicles in Cancer
inferred← citeshttps://doi.org/10.3390/cancers13020307openalex-snapshot
3D Cell Cultures as Prospective Models to Study Extracellular Vesicles in Cancer
inferred← citeshttps://doi.org/10.3390/cells11020186openalex-snapshot
Methodologies to Isolate and Purify Clinical Grade Extracellular Vesicles for Medical Applications
inferred← citeshttps://doi.org/10.3390/hemato3020026openalex-snapshot
miR-494 in Extracellular Vesicles as a Potent Biomarker of Chronic Myeloid Leukemia Treatment with Tyrosine Kinase Inhibitors
inferred← citeshttps://doi.org/10.3390/ijms19123859openalex-snapshot
A Promising Biocompatible Platform: Lipid-Based and Bio-Inspired Smart Drug Delivery Systems for Cancer Therapy
inferred← citeshttps://doi.org/10.3390/ijms20184597openalex-snapshot
Insights into the Secretome of Mesenchymal Stem Cells and Its Potential Applications
inferred← citeshttps://doi.org/10.3390/ijms21093071openalex-snapshot
Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
inferred← citeshttps://doi.org/10.3390/ijms21155432openalex-snapshot
Extracellular Vesicles-Based Drug Delivery Systems: A New Challenge and the Exemplum of Malignant Pleural Mesothelioma
inferred← citeshttps://doi.org/10.3390/ijms21186466openalex-snapshot
A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?
inferred← citeshttps://doi.org/10.3390/ijms23042321openalex-snapshot
Platelet-Released Factors: Their Role in Viral Disease and Applications for Extracellular Vesicle (EV) Therapy
inferred← citeshttps://doi.org/10.3390/ijms23052461openalex-snapshot
Exosomal RNAs: Novel Potential Biomarkers for Diseases—A Review
inferred← citeshttps://doi.org/10.3390/ijms24043663openalex-snapshot
Size-Exclusion Chromatography Combined with Ultrafiltration Efficiently Isolates Extracellular Vesicles from Human Blood Samples in Health and Disease
inferred← citeshttps://doi.org/10.3390/ijms242316983openalex-snapshot
Proton Compared to X-Irradiation Induces Different Protein Profiles in Oral Cancer Cells and Their Derived Extracellular Vesicles
inferred← citeshttps://doi.org/10.3390/microbiolres15040144openalex-snapshot
Fungal Extracellular Vesicles: Isolation, Characterization, and the Immune System Response
inferred← citeshttps://doi.org/10.3390/ncrna8060075openalex-snapshot
Exosome RNA Sequencing as a Tool in the Search for Cancer Biomarkers
inferred← citeshttps://doi.org/10.3390/ohbm2020006openalex-snapshot
Standardized Methodologies to Utilize Exosome Treatment as Potential Nano Substances in Hearing Loss
inferred← citeshttps://doi.org/10.3390/ph16030421openalex-snapshot
Endogenous Lipid Carriers—Bench-to-Bedside Roadblocks in Production and Drug Loading of Exosomes
inferred← citeshttps://doi.org/10.3390/ph18071005openalex-snapshot
Plant-Derived Exosomes: Nano-Inducers of Cross-Kingdom Regulations
inferred← citeshttps://doi.org/10.3390/pharmaceutics15020365openalex-snapshot
Extracellular Vesicles as New Players in Drug Delivery: A Focus on Red Blood Cells-Derived EVs
inferred← citeshttps://doi.org/10.3390/pharmaceutics15051453openalex-snapshot
Emerging Roles of Mesenchymal Stem/Stromal-Cell-Derived Extracellular Vesicles in Cancer Therapy
inferred← citeshttps://doi.org/10.3390/pharmaceutics16091206openalex-snapshot
Characterizing Extracellular Vesicles Generated from the Integra CELLine Culture System and Their Endocytic Pathways for Intracellular Drug Delivery
inferred← citeshttps://doi.org/10.3390/pharmaceutics17121617openalex-snapshot
Redefining the Limits of Nanodevices-Based Drug Delivery Systems: Extracellular Vesicles
inferred← citeshttps://doi.org/10.3390/s21227645openalex-snapshot
Advances in Biosensors Technology for Detection and Characterization of Extracellular Vesicles
inferred← citeshttps://doi.org/10.38212/2224-6614.3409openalex-snapshot
Exosomal proteomics in kidney disease: From technical approaches to clinical applications
inferred← citeshttps://doi.org/10.4308/hjb.30.6.1100-1110openalex-snapshot
The Properties of Exosomes Derived from Mesenchymal Stem Cells Preconditioned with L-Ascorbic Acid and Cobalt (II) Chloride
inferred← citeshttps://doi.org/10.51123/jgehes.2023.90openalex-snapshot
Hücre Dışı Veziküller ve Eksozom
inferred← citeshttps://doi.org/10.55230/mabjournal.v54i1.3357openalex-snapshot
Evaluation of Small Extracellular Vesicles Isolation Methods from Human Serum for Downstream miRNA Profiling
inferred← citeshttps://doi.org/10.5772/intechopen.109671openalex-snapshot
3D Culturing of Stem Cells: An Emerging Technique for Advancing Fundamental Research in Regenerative Medicine
inferred← citeshttps://doi.org/10.6084/m9.figshare.16305837openalex-snapshot
Additional file 1 of Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers
inferred← citeshttps://doi.org/10.70675/4a19f2b7zeb7az4fd0z8b88z5bbbdac0688dopenalex-snapshot
Activités procoagulante et fibrinolytique des microvésicules : de leur rôle dans la physiopathologie du sepsis au développement de nouveaux tests de diagnostic in vitro en hémostase
inferred← citeshttps://doi.org/10.70675/80b177bbzbe04z48adza9ccz4c8c5edcceacopenalex-snapshot
Novel electrokinetic and analytical approaches for isolation and detection of extracellular vesicles
inferred← citeshttps://doi.org/10.70675/b19bf813z271cz4793z95ffz75a631f85d34openalex-snapshot
Le sécrétome des bactéries du genre Mycoplasma ˸ focus sur les protéases sécrétées et les vésicules extracellulaires produites chez les mycoplasmes des ruminants.
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