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Ethidium Monoazide for DNA-Based Differentiation of Viable and Dead Bacteria by 5′-Nuclease PCR

https://doi.org/10.2144/03344rr02
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Detection of Heat Injury in Listeria monocytogenes Scott A

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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inferred← citeshttps://doi.org/10.1016/j.micres.2025.128224openalex-snapshot
Sources of variability for viability PCR using propidium monoazide
inferred← citeshttps://doi.org/10.1016/j.microc.2013.09.017openalex-snapshot
Legionella spp. survival after different disinfection procedures: Comparison between conventional culture, qPCR and EMA–qPCR
inferred← citeshttps://doi.org/10.1016/j.microc.2024.110296openalex-snapshot
Iontolysis using an electrically excited solid source of aqueous copper ions compatible with bacterial gene quantification
inferred← citeshttps://doi.org/10.1016/j.microc.2025.114594openalex-snapshot
Vancomycin-functionalized magnetic bead-qPCR platform for rapid differentiation of viable and dead Listeria monocytogenes in food samples
inferred← citeshttps://doi.org/10.1016/j.microc.2026.116977openalex-snapshot
Rapid detection of intact pathogens on a microfluidic device by combining immunomagnetic separation and viability dyes
inferred← citeshttps://doi.org/10.1016/j.mimet.2005.04.023openalex-snapshot
Discrimination of viable Vibrio vulnificus cells from dead cells in real-time PCR
inferred← citeshttps://doi.org/10.1016/j.mimet.2006.02.008openalex-snapshot
Real-time PCR for the detection of Salmonella spp. in food: An alternative approach to a conventional PCR system suggested by the FOOD-PCR project
inferred← citeshttps://doi.org/10.1016/j.mimet.2006.04.015openalex-snapshot
Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
inferred← citeshttps://doi.org/10.1016/j.mimet.2006.04.019openalex-snapshot
Use of ethidium bromide monoazide for quantification of viable and dead mixed bacterial flora from fish fillets by polymerase chain reaction
inferred← citeshttps://doi.org/10.1016/j.mimet.2006.11.011openalex-snapshot
A molecular beacon-based real time NASBA assay for detection of Listeria monocytogenes in food products: Role of target mRNA secondary structure on NASBA design
inferred← citeshttps://doi.org/10.1016/j.mimet.2007.09.005openalex-snapshot
Ethidium monoazide and propidium monoazide for elimination of unspecific DNA background in quantitative universal real-time PCR
inferred← citeshttps://doi.org/10.1016/j.mimet.2007.09.015openalex-snapshot
Differentiation of genes extracted from non-viable versus viable micro-organisms in environmental samples using ethidium monoazide bromide
inferred← citeshttps://doi.org/10.1016/j.mimet.2008.08.008openalex-snapshot
A comparative study of the ability of EMA and PMA to distinguish viable from heat killed mixed bacterial flora from fish fillets
inferred← citeshttps://doi.org/10.1016/j.mimet.2008.11.004openalex-snapshot
Selective detection of live bacteria combining propidium monoazide sample treatment with microarray technology
inferred← citeshttps://doi.org/10.1016/j.mimet.2011.02.004openalex-snapshot
Quantification of viable Legionella pneumophila cells using propidium monoazide combined with quantitative PCR
inferred← citeshttps://doi.org/10.1016/j.mimet.2011.07.016openalex-snapshot
Effect of PCR amplicon length on suppressing signals from membrane-compromised cells by propidium monoazide treatment
inferred← citeshttps://doi.org/10.1016/j.mimet.2012.08.007openalex-snapshot
Progress in understanding preferential detection of live cells using viability dyes in combination with DNA amplification
inferred← citeshttps://doi.org/10.1016/j.mimet.2013.01.018openalex-snapshot
Improving efficiency of viability-PCR for selective detection of live cells
inferred← citeshttps://doi.org/10.1016/j.mimet.2013.06.003openalex-snapshot
Propidium monoazide does not fully inhibit the detection of dead Campylobacter on broiler chicken carcasses by qPCR
inferred← citeshttps://doi.org/10.1016/j.mimet.2013.06.009openalex-snapshot
DNase I and Proteinase K eliminate DNA from injured or dead bacteria but not from living bacteria in microbial reference systems and natural drinking water biofilms for subsequent molecular biology analyses
inferred← citeshttps://doi.org/10.1016/j.mimet.2014.04.012openalex-snapshot
Enumeration of probiotic strains: Review of culture-dependent and alternative techniques to quantify viable bacteria
inferred← citeshttps://doi.org/10.1016/j.mimet.2015.03.007openalex-snapshot
Monitoring Campylobacter in the poultry production chain — From culture to genes and beyond
inferred← citeshttps://doi.org/10.1016/j.mimet.2015.07.012openalex-snapshot
Application of ethidium bromide monoazide for quantification of viable and dead cells of Salmonella enterica by real-time loop-mediated isothermal amplification
inferred← citeshttps://doi.org/10.1016/j.mimet.2016.01.015openalex-snapshot
Evaluation of two methods for direct detection of Fusarium spp. in water
inferred← citeshttps://doi.org/10.1016/j.mimet.2016.11.010openalex-snapshot
Most probable number - loop mediated isothermal amplification (MPN-LAMP) for quantifying waterborne pathogens in < 25 min
inferred← citeshttps://doi.org/10.1016/j.mimet.2018.12.003openalex-snapshot
Optimization of viability qPCR for selective detection of membrane-intact Legionella pneumophila
inferred← citeshttps://doi.org/10.1016/j.mimet.2019.03.008openalex-snapshot
Optimisation and validation of a PCR to detect viable Tenacibaculum maritimum in salmon skin tissue samples
inferred← citeshttps://doi.org/10.1016/j.mimet.2023.106737openalex-snapshot
Understanding the reaction balances behind the viability PCR protocols based on photoreactive dyes
inferred← citeshttps://doi.org/10.1016/j.mimet.2026.107568openalex-snapshot
Detection of viable and VBNC Salmonella in retail meat using optimized PMAxx real-time PCR
inferred← citeshttps://doi.org/10.1016/j.molliq.2019.111484openalex-snapshot
Removal of toxic chemical ethidium monoazide bromide using graphene oxide: Thermodynamic and kinetics study
inferred← citeshttps://doi.org/10.1016/j.resmic.2005.01.003openalex-snapshot
Quantification of Staphylococcus aureus in unpasteurised bovine and caprine milk by real-time PCR
inferred← citeshttps://doi.org/10.1016/j.resmic.2007.02.008openalex-snapshot
Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR
inferred← citeshttps://doi.org/10.1016/j.scitotenv.2008.03.004openalex-snapshot
Development and application of an oligonucleotide microarray and real-time quantitative PCR for detection of wastewater bacterial pathogens
inferred← citeshttps://doi.org/10.1016/j.scitotenv.2013.01.065openalex-snapshot
Discrimination of viable from non-viable Gram-negative bacterial pathogens in airborne particles using propidium monoazide-assisted qPCR
inferred← citeshttps://doi.org/10.1016/j.snb.2014.02.003openalex-snapshot
A fiber optic biosensor for specific identification of dead Escherichia coli O157:H7
inferred← citeshttps://doi.org/10.1016/j.talanta.2025.128193openalex-snapshot
Microplate-based impedance and thermal sensing system for concurrent cell viability and counting analysis
inferred← citeshttps://doi.org/10.1016/j.tifs.2011.02.009openalex-snapshot
The challenge of merging food safety diagnostic needs with quantitative PCR platforms
inferred← citeshttps://doi.org/10.1016/j.tifs.2024.104573openalex-snapshot
From farm to fork: Fungal and bacterial contaminants and their diagnostics in the production steps of ready-to-eat salads
inferred← citeshttps://doi.org/10.1016/j.trac.2024.118036openalex-snapshot
Latest trends in biosensors powered by nucleic acid isothermal amplification for the diagnosis of joint infections: From sampling to identification towards the point-of-care
inferred← citeshttps://doi.org/10.1016/j.tube.2014.11.005openalex-snapshot
Utility of propidium monoazide viability assay as a biomarker for a tuberculosis disease
inferred← citeshttps://doi.org/10.1016/j.vaccine.2006.07.013openalex-snapshot
Novel methods of quantitative real-time PCR data analysis in a murine Helicobacter pylori vaccine model
inferred← citeshttps://doi.org/10.1016/j.watres.2009.05.031openalex-snapshot
Quantitative real-time PCR analysis of total and propidium monoazide-resistant fecal indicator bacteria in wastewater
inferred← citeshttps://doi.org/10.1016/j.watres.2016.02.016openalex-snapshot
Determination of viable legionellae in engineered water systems: Do we find what we are looking for?
inferred← citeshttps://doi.org/10.1016/j.wroa.2020.100080openalex-snapshot
Capsid integrity quantitative PCR to determine virus infectivity in environmental and food applications – A systematic review
inferred← citeshttps://doi.org/10.1017/s0022029910000658openalex-snapshot
Rapid and direct quantitative detection of viable bifidobacteria in probiotic yogurt by combination of ethidium monoazide and real-time PCR using a molecular beacon approach
inferred← citeshttps://doi.org/10.1021/acs.analchem.4c02204openalex-snapshot
A Propidium Monoazide-Assisted Digital CRISPR/Cas12a Assay for Selective Detection of Live Bacteria in Sample
inferred← citeshttps://doi.org/10.1021/acs.chemrestox.4c00378openalex-snapshot
The Intercalator Ethidium Bromide Generates Covalent Adducts at Apurinic/Apyrimidinic Sites in DNA
inferred← citeshttps://doi.org/10.1021/bp060020qopenalex-snapshot
A Recombinant Bacteriophage-Based Assay for the Discriminative Detection of Culturable and Viable but Nonculturable Escherichia coli O157:H7
inferred← citeshttps://doi.org/10.1021/es071341gopenalex-snapshot
Development and Application of Real-Time PCR Assays for Quantifying Total and Aerolysin Gene-Containing Aeromonas in Source, Intermediate, and Finished Drinking Water
inferred← citeshttps://doi.org/10.1038/nrmicro3400openalex-snapshot
Ecology and exploration of the rare biosphere
inferred← citeshttps://doi.org/10.1038/s41598-018-36406-zopenalex-snapshot
Fate of bacterial indicators and Salmonella in biofilm developed on ultrafiltration membranes treating secondary effluents of domestic wastewater
inferred← citeshttps://doi.org/10.1038/s41598-020-80645-yopenalex-snapshot
Chlamydia trachomatis intra-bacterial and total plasmid copy number in clinical urogenital samples
inferred← citeshttps://doi.org/10.1039/d3an01750hopenalex-snapshot
Progress in methods for the detection of viable Escherichia coli
inferred← citeshttps://doi.org/10.1080/01652176.2025.2454473openalex-snapshot
Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens
inferred← citeshttps://doi.org/10.1080/07060661.2016.1216897openalex-snapshot
Rapid detection and quantification of viable Pseudomonas syringae pv. lachrymans cells in contaminated cucumber seeds using propidium monoazide and a real-time PCR assay
inferred← citeshttps://doi.org/10.1080/08905430701410548openalex-snapshot
Quantification of ViablePlesiomonas shigelloidesin a Mixture of Viable and Dead Cells Using Ethidium Bromide Monoazide and Conventional PCR
inferred← citeshttps://doi.org/10.1080/08905436.2010.507154openalex-snapshot
Selective Detection of Mixed Bacterial Survivors from Fish Fillets after Freezing and Thawing by Ethidium Bromide Monoazide Real-Time PCR
inferred← citeshttps://doi.org/10.1080/08905436.2014.996896openalex-snapshot
Recent Developments in Molecular Detection of Food-Borne Disease Bacteria and GMOs
inferred← citeshttps://doi.org/10.1080/10408398.2012.656771openalex-snapshot
Comparison of Molecular Techniques with other Methods for Identification and Enumeration of Probiotics in Fermented Milk Products
inferred← citeshttps://doi.org/10.1080/14737159.2018.1498785openalex-snapshot
Review ofChlamydia trachomatisviability methods: assessing the clinical diagnostic impact of NAAT positive results
inferred← citeshttps://doi.org/10.1089/ast.2014.1181openalex-snapshot
Detection and Classification of Live and Dead Escherichia coli by Laser-Induced Breakdown Spectroscopy
inferred← citeshttps://doi.org/10.1093/femsle/fnab148openalex-snapshot
Detection of viable Lacticaseibacillus paracasei in fermented milk using propidium monoazide combined with quantitative loop-mediated isothermal amplification
inferred← citeshttps://doi.org/10.1093/fqsafe/fyae051openalex-snapshot
Detection, assessment, and control strategies for managing Vibrio parahaemolyticus risk in seafood
inferred← citeshttps://doi.org/10.1093/gigascience/giaf100openalex-snapshot
Nanopore- and AI-empowered microbial viability inference
inferred← citeshttps://doi.org/10.1094/pbiomes-08-25-0059-scopenalex-snapshot
Assessing the Effectiveness of Propidium Monoazide on the Viable Bacterial Microbiome in Huanglongbing-Affected Citrus via 16S rRNA Amplicon Sequencing
inferred← citeshttps://doi.org/10.1094/pdis-92-5-0660openalex-snapshot
Nucleic Acid–Based Pathogen Detection in Applied Plant Pathology
inferred← citeshttps://doi.org/10.1094/phyto-04-20-0152-ropenalex-snapshot
Assessment of the Effect of Thermotherapy on ‘ Candidatus Liberibacter asiaticus’ Viability in Woody Tissue of Citrus via Graft-Based Assays and RNA Assays
inferred← citeshttps://doi.org/10.1094/phyto-05-13-0119-ropenalex-snapshot
Viability of ‘Candidatus Liberibacter asiaticus’ Prolonged by Addition of Citrus Juice to Culture Medium
inferred← citeshttps://doi.org/10.1094/phyto-10-23-0366-iaopenalex-snapshot
New Approaches to Plant Pathogen Detection and Disease Diagnosis
inferred← citeshttps://doi.org/10.1099/mic.0.000786openalex-snapshot
Assessment of bacterial viability: a comprehensive review on recent advances and challenges
inferred← citeshttps://doi.org/10.1101/2024.06.10.598221openalex-snapshot
Nanopore- and AI-empowered microbial viability inference
inferred← citeshttps://doi.org/10.1111/1541-4337.12072openalex-snapshot
Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection
inferred← citeshttps://doi.org/10.1111/1541-4337.12074openalex-snapshot
Culture‐Dependent and Culture‐Independent Nucleic‐Acid‐Based Methods Used in the Microbial Safety Assessment of Milk and Dairy Products
inferred← citeshttps://doi.org/10.1111/1541-4337.13153openalex-snapshot
Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges
inferred← citeshttps://doi.org/10.1111/1751-7915.13039openalex-snapshot
Molecular viability testing of viable but non‐culturable bacteria induced by antibiotic exposure
inferred← citeshttps://doi.org/10.1111/1758-2229.12786openalex-snapshot
New strategies for the enumeration of enteric pathogens in water
inferred← citeshttps://doi.org/10.1111/j.1348-0421.2007.tb03966.xopenalex-snapshot
Photoactivated Ethidium Monoazide Directly Cleaves Bacterial DNA and Is Applied to PCR for Discrimination of Live and Dead Bacteria
inferred← citeshttps://doi.org/10.1111/j.1348-0421.2011.00388.xopenalex-snapshot
Selective detection of viable Helicobacter pylori using ethidium monoazide or propidium monoazide in combination with real-time polymerase chain reaction
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2005.02728.xopenalex-snapshot
Rapid differentiation and enumeration of the total, viable vegetative cell and spore content of thermophilic bacilli in milk powders with reference to Anoxybacillus flavithermus
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2007.03589.xopenalex-snapshot
Discrimination of γ-irradiated and nonirradiated Vibrio vulnificus by using real-time polymerase chain reaction
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2008.03851.xopenalex-snapshot
Selective PCR detection of viableEnterobacter sakazakiicells utilizing propidium monoazide or ethidium bromide monoazide
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2009.04358.xopenalex-snapshot
Detection of viableEscherichia coliO157:H7 by ethidium monoazide real-time PCR
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2010.04716.xopenalex-snapshot
New approach to use ethidium bromide monoazide as an analytical tool
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2010.04830.xopenalex-snapshot
Feasibility of methods based on nucleic acid amplification techniques to fulfil the requirements for microbiological analysis of water quality
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2011.05125.xopenalex-snapshot
The effect of various environmental factors on the ethidium monazite and quantitative PCR method to detect viable bacteria
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2011.05215.xopenalex-snapshot
Differentiation of live, dead and treated cells of Escherichia coli O157:H7 using FT-IR spectroscopy
inferred← citeshttps://doi.org/10.1111/j.1365-2672.2012.05382.xopenalex-snapshot
Long-amplicon propidium monoazide-PCR enumeration assay to detect viable Campylobacter and Salmonella
inferred← citeshttps://doi.org/10.1111/j.1365-3059.2007.01736.xopenalex-snapshot
Quantification of viable cells of Clavibacter michiganensis subsp. michiganensis using a DNA binding dye and a real‐time PCR assay
inferred← citeshttps://doi.org/10.1111/j.1472-765x.2005.01672.xopenalex-snapshot
Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR
inferred← citeshttps://doi.org/10.1111/j.1472-765x.2009.02707.xopenalex-snapshot
The presence ofSaccharomyces cerevisiaeDNA in various media used to propagate yeasts and its removal by ethidium monoazide
inferred← citeshttps://doi.org/10.1111/j.1574-6968.2008.01429.xopenalex-snapshot
Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques
inferred← citeshttps://doi.org/10.1111/j.1574-6968.2009.01549.xopenalex-snapshot
Discrimination of live, anti-tuberculosis agent-injured, and deadMycobacterium tuberculosisusing flow cytometry
inferred← citeshttps://doi.org/10.1111/j.1600-0463.2011.02767.xopenalex-snapshot
Rapid detection, characterization, and enumeration of foodborne pathogens
inferred← citeshttps://doi.org/10.1111/j.1600-0765.2012.01522.xopenalex-snapshot
Analysis of viable vs. dead A ggregatibacter actinomycetemcomitans and P orphyromonas gingivalis using selective quantitative real‐time PCR with propidium monoazide
inferred← citeshttps://doi.org/10.1111/j.1750-3841.2010.01525.xopenalex-snapshot
EMA‐Real‐Time PCR as a Reliable Method for Detection of Viable Salmonella in Chicken and Eggs
inferred← citeshttps://doi.org/10.1111/j.1750-3841.2011.02123.xopenalex-snapshot
Detection of Viable Salmonella in Lettuce by Propidium Monoazide Real‐Time PCR
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