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A protocol for targeted enrichment of intron‐containing sequence markers for recent radiations: A phylogenomic example from Heuchera (Saxifragaceae)

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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CHLOROPLAST DNA VARIATION WITHIN AND AMONG GENERA OF THE HEUCHERA GROUP (SAXIFRAGACEAE): EVIDENCE FOR CHLOROPLAST TRANSFER AND PARAPHYLY
inferredcites →https://doi.org/10.1007/978-1-59745-251-9_3openalex-snapshot
Multiple Alignment of DNA Sequences with MAFFT
inferredcites →https://doi.org/10.1016/j.ympev.2011.12.007openalex-snapshot
Applications of next-generation sequencing to phylogeography and phylogenetics
inferredcites →https://doi.org/10.1016/j.ympev.2014.08.013openalex-snapshot
Phylogenetic analysis at deep timescales: Unreliable gene trees, bypassed hidden support, and the coalescence/concatalescence conundrum
inferredcites →https://doi.org/10.1016/j.ympev.2014.11.011openalex-snapshot
Phylogeny, divergence times, and historical biogeography of the angiosperm family Saxifragaceae
inferredcites →https://doi.org/10.1038/35048692openalex-snapshot
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
inferredcites →https://doi.org/10.1038/nbt.1523openalex-snapshot
Solution hybrid selection with ultra-long oligonucleotides for massively parallel targeted sequencing
inferredcites →https://doi.org/10.1038/ng.911openalex-snapshot
Whole-genome sequencing of multiple Arabidopsis thaliana populations
inferredcites →https://doi.org/10.1046/j.1365-294x.2001.01285.xopenalex-snapshot
Chloroplast microsatellites and mitochondrial nad1 intron 2 sequences indicate congruent phylogenetic relationships among Swiss stone pine (Pinus cembra), Siberian stone pine (Pinus sibirica), and Siberian dwarf pine (Pinus pumila)
inferredcites →https://doi.org/10.1073/pnas.1232297100openalex-snapshot
Large-scale comparison of intron positions in mammalian genes shows intron loss but no gain
inferredcites →https://doi.org/10.1073/pnas.97.8.4086openalex-snapshot
Seed plant phylogeny inferred from all three plant genomes: Monophyly of extant gymnosperms and origin of Gnetales from conifers
inferredcites →https://doi.org/10.1080/10409230290771474openalex-snapshot
Utility of Low-Copy Nuclear Gene Sequences in Plant Phylogenetics
inferredcites →https://doi.org/10.1080/10635150801888871openalex-snapshot
Resolving an Ancient, Rapid Radiation in Saxifragales
inferredcites →https://doi.org/10.1093/bioinformatics/bth058openalex-snapshot
Gene structure prediction from consensus spliced alignment of multiple ESTs matching the same genomic locus
inferredcites →https://doi.org/10.1093/bioinformatics/btl446openalex-snapshot
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models
inferredcites →https://doi.org/10.1093/bioinformatics/btp324openalex-snapshot
Fast and accurate short read alignment with Burrows–Wheeler transform
inferredcites →https://doi.org/10.1093/bioinformatics/btu170openalex-snapshot
Trimmomatic: a flexible trimmer for Illumina sequence data
inferredcites →https://doi.org/10.1093/molbev/msn226openalex-snapshot
Mitochondrial DNA of Vitis vinifera and the Issue of Rampant Horizontal Gene Transfer
inferredcites →https://doi.org/10.1093/nar/gkt289openalex-snapshot
OrganellarGenomeDRAW—a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets
inferredcites →https://doi.org/10.1093/nar/gkt377openalex-snapshot
STRAW: Species TRee Analysis Web server
inferredcites →https://doi.org/10.1093/sysbio/syp031openalex-snapshot
Estimating Species Phylogenies Using Coalescence Times among Sequences
inferredcites →https://doi.org/10.1101/gr.074492.107openalex-snapshot
Velvet: Algorithms for de novo short read assembly using de Bruijn graphs
inferredcites →https://doi.org/10.1101/gr.2289704openalex-snapshot
Mauve: Multiple Alignment of Conserved Genomic Sequence With Rearrangements
inferredcites →https://doi.org/10.1111/j.1365-294x.2007.03640.xopenalex-snapshot
Ancient hybridization and mitochondrial capture between two species of chipmunks
inferredcites →https://doi.org/10.1111/j.1558-5646.1995.tb02309.xopenalex-snapshot
DISCORDANCE BETWEEN NUCLEAR AND CHLOROPLAST PHYLOGENIES IN THE HEUCHERA GROUP (SAXIFRAGACEAE)
inferredcites →https://doi.org/10.1111/nph.12395openalex-snapshot
One ring to rule them all? Genome sequencing provides new insights into the ‘master circle’ model of plant mitochondrial DNA structure
inferredcites →https://doi.org/10.1186/1471-2148-10-302openalex-snapshot
A maximum pseudo-likelihood approach for estimating species trees under the coalescent model
inferredcites →https://doi.org/10.1186/1471-2148-14-23openalex-snapshot
From algae to angiosperms–inferring the phylogeny of green plants (Viridiplantae) from 360 plastid genomes
inferredcites →https://doi.org/10.1186/2047-217x-1-18openalex-snapshot
SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler
inferredcites →https://doi.org/10.1371/journal.pone.0016404openalex-snapshot
The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats
inferredcites →https://doi.org/10.1371/journal.pone.0109704openalex-snapshot
Phylogenetic Properties of 50 Nuclear Loci in Medicago (Leguminosae) Generated Using Multiplexed Sequence Capture and Next-Generation Sequencing
inferredcites →https://doi.org/10.2307/25027594openalex-snapshot
A Revision of the Genus Heuchera (Saxifragaceae) in Eastern North America
inferredcites →https://doi.org/10.3732/ajb.1100335openalex-snapshot
Navigating the tip of the genomic iceberg: Next‐generation sequencing for plant systematics
inferredcites →https://doi.org/10.3732/ajb.1100356openalex-snapshot
Targeted enrichment strategies for next‐generation plant biology
inferredcites →https://doi.org/10.3732/ajb.1200275openalex-snapshot
Polyploidization in Heuchera cylindrica (Saxifragaceae) did not result in a shift in climatic requirements
inferredcites →https://doi.org/10.3732/ajb.1400290openalex-snapshot
Phylogenetic relationships and character evolution in Heuchera (Saxifragaceae) on the basis of multiple nuclear loci
inferredcites →https://doi.org/10.3732/ajb.1500026openalex-snapshot
Comparison of nuclear, plastid, and mitochondrial phylogenies and the origin of wild octoploid strawberry species
inferredcites →https://doi.org/10.3732/apps.1300085openalex-snapshot
A target enrichment method for gathering phylogenetic information from hundreds of loci: An example from the Compositae
inferredcites →https://doi.org/10.3732/apps.1400040openalex-snapshot
Microsatellite primers for the gynodioecious grassland perennial Saxifraga granulata (Saxifragaceae)
inferredcites →https://doi.org/10.3732/apps.1400042openalex-snapshot
Hyb‐Seq: Combining target enrichment and genome skimming for plant phylogenomics
inferredcites →https://doi.org/10.3732/apps.1400062openalex-snapshot
Geneious! Simplified genome skimming methods for phylogenetic systematic studies: A case study in Oreocarya (Boraginaceae)
inferredcites →https://doi.org/10.48550/arxiv.1303.3997openalex-snapshot
Aligning sequence reads, clone sequences and assembly contigs with BWA-MEM
inferredcites →https://doi.org/10.6084/m9.figshare.963153.v1openalex-snapshot
Aligning sequence reads, clone sequences and assembly con*gs with BWA-MEM

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1002/tax.12421zenodo-depositor-declared
Phylogenomics of the tropical plant family Ochnaceae using targeted enrichment of nuclear genes and 250+ taxa
inferred← citeshttps://doi.org/10.24823/ejb.2022.409zenodo-depositor-declared
A HYBRID CAPTURE BAIT SET FOR BEGONIA
inferred← citeshttps://doi.org/10.3897/phytokeys.251.136373zenodo-depositor-declared
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inferred← citeshttps://doi.org/10.1002/ajb2.1059openalex-snapshot
Challenges of comprehensive taxon sampling in comparative biology: Wrestling with rosids
inferred← citeshttps://doi.org/10.1002/aps3.1036openalex-snapshot
Targeting legume loci: A comparison of three methods for target enrichment bait design in Leguminosae phylogenomics
inferred← citeshttps://doi.org/10.1002/aps3.1038openalex-snapshot
Practical considerations for plant phylogenomics
inferred← citeshttps://doi.org/10.1002/aps3.11254openalex-snapshot
A customized nuclear target enrichment approach for developing a phylogenomic baseline for Dioscorea yams (Dioscoreaceae)
inferred← citeshttps://doi.org/10.1002/aps3.11295openalex-snapshot
An empirical assessment of a single family‐wide hybrid capture locus set at multiple evolutionary timescales in Asteraceae
inferred← citeshttps://doi.org/10.1002/aps3.11345openalex-snapshot
A two‐tier bioinformatic pipeline to develop probes for target capture of nuclear loci with applications in Melastomataceae
inferred← citeshttps://doi.org/10.1002/aps3.11410openalex-snapshot
High‐throughput methods for efficiently building massive phylogenies from natural history collections
inferred← citeshttps://doi.org/10.1002/aps3.11438openalex-snapshot
The best of both worlds: Combining lineage‐specific and universal bait sets in target‐enrichment hybridization reactions
inferred← citeshttps://doi.org/10.1002/aps3.11537openalex-snapshot
Target capture and genome skimming for plant diversity studies
inferred← citeshttps://doi.org/10.1002/aps3.11568openalex-snapshot
Compositae‐ParaLoss‐1272: A complementary sunflower‐specific probe set reduces paralogs in phylogenomic analyses of complex systems
inferred← citeshttps://doi.org/10.1002/aps3.70019openalex-snapshot
Advancing phylogenomics in Amaranthaceae sensu stricto: Development and application of a new nuclear target enrichment bait set
inferred← citeshttps://doi.org/10.1002/tax.12365openalex-snapshot
Phylogenomics supported by geometric morphometrics reveals delimitation of sexual species within the polyploid apomictic Ranunculus auricomus complex (Ranunculaceae)
inferred← citeshttps://doi.org/10.1002/tax.12450openalex-snapshot
Biogeography and habitat evolution of Saxifragaceae, with a revision of generic limits and a new tribal system
inferred← citeshttps://doi.org/10.1007/978-1-0716-3838-5_11openalex-snapshot
A Guide to Phylogenomic Inference
inferred← citeshttps://doi.org/10.1007/978-1-4939-7463-4_5openalex-snapshot
Phylogenomics
inferred← citeshttps://doi.org/10.1007/s44281-023-00026-zopenalex-snapshot
Integrating genomic sequencing resources: an innovative perspective on recycling with universal Angiosperms353 probe sets
inferred← citeshttps://doi.org/10.1016/j.ympev.2017.02.006openalex-snapshot
A pilot study applying the plant Anchored Hybrid Enrichment method to New World sages (Salvia subgenus Calosphace; Lamiaceae)
inferred← citeshttps://doi.org/10.1016/j.ympev.2017.04.003openalex-snapshot
Deep reticulation and incomplete lineage sorting obscure the diploid phylogeny of rain-lilies and allies (Amaryllidaceae tribe Hippeastreae)
inferred← citeshttps://doi.org/10.1016/j.ympev.2018.02.016openalex-snapshot
Pseudo-parallel patterns of disjunctions in an Arctic-alpine plant lineage
inferred← citeshttps://doi.org/10.1016/j.ympev.2018.07.012openalex-snapshot
Exploring data processing strategies in NGS target enrichment to disentangle radiations in the tribe Cardueae (Compositae)
inferred← citeshttps://doi.org/10.1016/j.ympev.2018.07.020openalex-snapshot
Resolving the rapid plant radiation of early diverging lineages in the tropical Zingiberales: Pushing the limits of genomic data
inferred← citeshttps://doi.org/10.1016/j.ympev.2019.05.001openalex-snapshot
Nuclear and plastid DNA phylogeny of tribe Cardueae (Compositae) with Hyb-Seq data: A new subtribal classification and a temporal diversification framework
inferred← citeshttps://doi.org/10.1016/j.ympev.2020.106769openalex-snapshot
Utility of targeted sequence capture for phylogenomics in rapid, recent angiosperm radiations: Neotropical Burmeistera bellflowers as a case study
inferred← citeshttps://doi.org/10.1016/j.ympev.2020.106899openalex-snapshot
Larger, unfiltered datasets are more effective at resolving phylogenetic conflict: Introns, exons, and UCEs resolve ambiguities in Golden-backed frogs (Anura: Ranidae; genus Hylarana)
inferred← citeshttps://doi.org/10.1016/j.ympev.2021.107070openalex-snapshot
Molecular phylogeny and ancestral biogeographic reconstruction of Platanthera subgenus Limnorchis (Orchidaceae) using target capture methods
inferred← citeshttps://doi.org/10.1016/j.ympev.2021.107202openalex-snapshot
Development and phylogenetic utilities of a new set of single-/low-copy nuclear genes in Senecioneae (Asteraceae), with new insights into the tribal position and the relationships within subtribe Tussilagininae
inferred← citeshttps://doi.org/10.1016/j.ympev.2025.108445openalex-snapshot
Target sequence capture informs generic delimitation and hybridization in the tribe Lasiopetaleae (Malvaceae)
inferred← citeshttps://doi.org/10.1080/23802359.2025.2529830openalex-snapshot
The complete chloroplast genome of Mitella nuda (Saxifragaceae)
inferred← citeshttps://doi.org/10.1086/698120openalex-snapshot
Geographic Range Dynamics Drove Ancient Hybridization in a Lineage of Angiosperms
inferred← citeshttps://doi.org/10.1086/715636openalex-snapshot
Discordance in a South African Memecylon Clade (Melastomataceae): Evidence for Reticulate Evolution
inferred← citeshttps://doi.org/10.1093/botlinnean/boy012openalex-snapshot
Integrative identification of incipient lineages in Heuchera longiflora (Saxifragaceae)
inferred← citeshttps://doi.org/10.1093/gbe/evad070openalex-snapshot
Filtration of Gene Trees From 9,000 Exons, Introns, and UCEs Disentangles Conflicting Phylogenomic Relationships in Tree Frogs (Hylidae)
inferred← citeshttps://doi.org/10.1093/sysbio/syac032openalex-snapshot
Target Enrichment and Extensive Population Sampling Help Untangle the Recent, Rapid Radiation of Oenothera Sect. Calylophus
inferred← citeshttps://doi.org/10.1093/sysbio/syad018openalex-snapshot
Identifying Climatic Drivers of Hybridization with a New Ancestral Niche Reconstruction Method
inferred← citeshttps://doi.org/10.1093/sysbio/syw083openalex-snapshot
Ancestral Gene Flow and Parallel Organellar Genome Capture Result in Extreme Phylogenomic Discord in a Lineage of Angiosperms
inferred← citeshttps://doi.org/10.1101/2021.08.30.458250openalex-snapshot
Unraveling Phylogenetic Relationships, Reticulate Evolution, and Genome Composition of Polyploid Plant Complexes by Rad-Seq and Hyb-Seq
inferred← citeshttps://doi.org/10.1101/2022.01.10.474775openalex-snapshot
Optimization of capture protocols across species targeting up to 32000 genes and their extension to pooled DNA
inferred← citeshttps://doi.org/10.1101/2022.08.24.505154openalex-snapshot
Identifying climatic drivers of hybridization in Heuchereae (Saxifragaceae)
inferred← citeshttps://doi.org/10.1101/765610openalex-snapshot
Exons, Introns, and UCEs Reveal Conflicting Phylogenomic Signals in a Rapid Radiation of Frogs (Ranidae: Hylarana )
inferred← citeshttps://doi.org/10.1101/832683openalex-snapshot
Species delimitation in the grey zone: introgression obfuscates phylogenetic inference and species boundaries in a cryptic frog complex (Ranidae: Pulchrana picturata )
inferred← citeshttps://doi.org/10.1111/1755-0998.13327openalex-snapshot
A multitiered sequence capture strategy spanning broad evolutionary scales: Application for phylogenetic and phylogeographic studies of orchids
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Taxon‐specific or universal? Using target capture to study the evolutionary history of rapid radiations
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An introduction to plant phylogenomics with a focus on palms
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Genomic data reveals potential for hybridization, introgression, and incomplete lineage sorting to confound phylogenetic relationships in an adaptive radiation of narrow‐mouth frogs
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Nuclear phylogenomics of angiosperms and insights into their relationships and evolution
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Diversification of Hawaiian Cyrtandra (Gesneriaceae) under the influence of incomplete lineage sorting and hybridization
inferred← citeshttps://doi.org/10.1111/jse.12646openalex-snapshot
Biogeography and ecological niche evolution in Diapensiaceae inferred from phylogenetic analysis
inferred← citeshttps://doi.org/10.1111/jse.12745openalex-snapshot
A phylogenomic perspective on gene tree conflict and character evolution in Caprifoliaceae using target enrichment data, with Zabelioideae recognized as a new subfamily
inferred← citeshttps://doi.org/10.1111/jse.12806openalex-snapshot
Capturing single‐copy nuclear genes, organellar genomes, and nuclear ribosomal DNA from deep genome skimming data for plant phylogenetics: A case study in Vitaceae
inferred← citeshttps://doi.org/10.1111/jse.12849openalex-snapshot
Stable persistence of relict populations involved evolutionary shifts of reproductive characters in the genus Tanakaea (Saxifragaceae)
inferred← citeshttps://doi.org/10.1111/mec.15458openalex-snapshot
Phylogenomics unravels Quaternary vicariance and allopatric speciation patterns in temperate‐montane plant species: A case study on the Ranunculus auricomus species complex
inferred← citeshttps://doi.org/10.1111/nph.15099openalex-snapshot
Phylogenomic analyses reveal a deep history of hybridization and polyploidy in the Neotropical genus Lachemilla (Rosaceae)
inferred← citeshttps://doi.org/10.1111/nph.18284openalex-snapshot
Untying Gordian knots: unraveling reticulate polyploid plant evolution by genomic data using the large Ranunculus auricomus species complex
inferred← citeshttps://doi.org/10.11606/t.11.2020.tde-04062020-160041openalex-snapshot
Chloroplast genome evolution in >i /i<
inferred← citeshttps://doi.org/10.1177/1176934317742613openalex-snapshot
HybPhyloMaker: Target Enrichment Data Analysis From Raw Reads to Species Trees
inferred← citeshttps://doi.org/10.1186/s12870-020-02533-xopenalex-snapshot
Degradation of key photosynthetic genes in the critically endangered semi-aquatic flowering plant Saniculiphyllum guangxiense (Saxifragaceae)
inferred← citeshttps://doi.org/10.1600/036364423x16847773873071openalex-snapshot
Morphometrics and Phylogenomics Reveal a New Species ofHeuchera(Saxifragaceae) in Northeastern México
inferred← citeshttps://doi.org/10.1600/036364424x17110456120677openalex-snapshot
Heuchera tuckasegeensissp. nov. (Saxifragaceae), a New Species from Western North Carolina
inferred← citeshttps://doi.org/10.3389/fgene.2019.01407openalex-snapshot
A Guide to Carrying Out a Phylogenomic Target Sequence Capture Project
inferred← citeshttps://doi.org/10.3389/fpls.2017.00334openalex-snapshot
The Chloroplast Genome of Passiflora edulis (Passifloraceae) Assembled from Long Sequence Reads: Structural Organization and Phylogenomic Studies in Malpighiales
inferred← citeshttps://doi.org/10.3389/fpls.2019.01224openalex-snapshot
Phylogenomics Yields New Insight Into Relationships Within Vernonieae (Asteraceae)
inferred← citeshttps://doi.org/10.3389/fpls.2019.01773openalex-snapshot
A Phylogenomic Perspective on Evolution and Discordance in the Alpine-Arctic Plant Clade Micranthes (Saxifragaceae)
inferred← citeshttps://doi.org/10.3389/fpls.2020.00361openalex-snapshot
Plastome Evolution in Saxifragaceae and Multiple Plastid Capture Events Involving Heuchera and Tiarella
inferred← citeshttps://doi.org/10.3389/fpls.2021.765719openalex-snapshot
How to Tackle Phylogenetic Discordance in Recent and Rapidly Radiating Groups? Developing a Workflow Using Loricaria (Asteraceae) as an Example
inferred← citeshttps://doi.org/10.3389/fpls.2022.851716openalex-snapshot
New Insights Into the Relationships Within Subtribe Scorzonerinae (Cichorieae, Asteraceae) Using Hybrid Capture Phylogenomics (Hyb-Seq)
inferred← citeshttps://doi.org/10.3389/fpls.2023.1219354openalex-snapshot
Plastid phylogenomics clarifies broad-level relationships in Bulbophyllum (Orchidaceae) and provides insights into range evolution of Australasian section Adelopetalum
inferred← citeshttps://doi.org/10.3390/biology11111661openalex-snapshot
The Mitogenome of Sedum plumbizincicola (Crassulaceae): Insights into RNA Editing, Lateral Gene Transfer, and Phylogenetic Implications
inferred← citeshttps://doi.org/10.3390/biology12030418openalex-snapshot
Geometric Morphometric Versus Genomic Patterns in a Large Polyploid Plant Species Complex
inferred← citeshttps://doi.org/10.3390/ijms20184534openalex-snapshot
Chloroplast Genomes and Comparative Analyses among Thirteen Taxa within Myrsinaceae s.str. Clade (Myrsinoideae, Primulaceae)
inferred← citeshttps://doi.org/10.3390/plants8100386openalex-snapshot
Next-Generation Genome Sequencing of Sedum plumbizincicola Sheds Light on the Structural Evolution of Plastid rRNA Operon and Phylogenetic Implications within Saxifragales
inferred← citeshttps://doi.org/10.3732/apps.1600016openalex-snapshot
HybPiper: Extracting coding sequence and introns for phylogenetics from high‐throughput sequencing reads using target enrichment
inferred← citeshttps://doi.org/10.3897/zookeys.1149.85627openalex-snapshot
Performance of intron 7 of the β-fibrinogen gene for phylogenetic analysis: An example using gladiator frogs, Boana Gray, 1825 (Anura, Hylidae, Cophomantinae)
inferred← citeshttps://doi.org/10.64898/2026.02.25.708067openalex-snapshot
Investigating the landscape of plant-pollinator interactions in a hybrid zone
inferred← citeshttps://doi.org/10.64898/2026.02.27.708378openalex-snapshot
The geographic structure of chloroplast capture in a hybrid zone
inferred← citeshttps://doi.org/10.64898/2026.02.27.708381openalex-snapshot
Species delimitation in an intractable syngameon: Bringing order to the polyphyletic Heuchera americana group
inferred← citeshttps://doi.org/10.7717/peerj.2101openalex-snapshot
Complete plastid genome sequence of Primula sinensis (Primulaceae): structure comparison, sequence variation and evidence for accD transfer to nucleus
inferred← citeshttps://doi.org/10.7717/peerj.3569openalex-snapshot
Targeted NGS for species level phylogenomics: “made to measure” or “one size fits all”?
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