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Metabarcoding reveals a high diversity of woody host-associated Phytophthora spp. in soils at public gardens and amenity woodlands in Britain

https://doi.org/10.7717/peerj.6931
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Outgoing claims: the papers this work cites, builds on, or argues with.

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Aligning Short Sequencing Reads with Bowtie
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Quantitative evaluation of bias in PCR amplification and next-generation sequencing derived from metabarcoding samples
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Urban environments provide opportunities for early detections of Phytophthora invasions
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Urban trees: bridge-heads for forest pest invasions and sentinels for early detection
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Phytophthora versiformis sp. nov., a new species from Australia related to P. quercina
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PCR protocols — A guide to methods and applications
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Cropping history shapes fungal, oomycete and nematode communities in arable soils and affects cavity spot in carrot
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Survival of Phytophthora cinnamomi as oospores, stromata, and thick-walled chlamydospores in roots of symptomatic and asymptomatic annual and herbaceous perennial plant species
inferredcites →https://doi.org/10.1016/j.funeco.2018.09.001openalex-snapshot
Community composition and distribution of Phytophthora species across adjacent native and non-native forests of South Africa
inferredcites →https://doi.org/10.1016/j.mimet.2011.12.012openalex-snapshot
A molecular method to assess Phytophthora diversity in environmental samples
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AFLPs detect low genetic diversity for Phytophthora nemorosa and P. pseudosyringae in the US and Europe
inferredcites →https://doi.org/10.1016/s0022-2836(05)80360-2openalex-snapshot
Basic Local Alignment Search Tool
inferredcites →https://doi.org/10.1016/s0261-2194(97)83220-6openalex-snapshot
Phytophthora diseases worldwide
inferredcites →https://doi.org/10.1017/s0953756202005622openalex-snapshot
Three new species of Phytophthora from European oak forests
inferredcites →https://doi.org/10.1017/s095375620300738xopenalex-snapshot
Multiple new phenotypic taxa from trees and riparian ecosystems in Phytophthora gonapodyides-P. megasperma ITS Clade 6, which tend to be high-temperature tolerant and either inbreeding or sterile
inferredcites →https://doi.org/10.1017/s0953756203008074openalex-snapshot
Phytophthora pseudosyringae sp. nov., a new species causing root and collar rot of deciduous tree species in Europe
inferredcites →https://doi.org/10.1017/s0953756205003357openalex-snapshot
Phytophthora kernoviae sp. nov., an invasive pathogen causing bleeding stem lesions on forest trees and foliar necrosis of ornamentals in the UK
inferredcites →https://doi.org/10.1023/a:1021208106857openalex-snapshot
Detection of Phytophthora nicotianae and P. citrophthora in Citrus Roots and Soils by Nested PCR
inferredcites →https://doi.org/10.1038/466824aopenalex-snapshot
Sudden larch death
inferredcites →https://doi.org/10.1038/s41396-018-0229-3openalex-snapshot
Contrasting distribution patterns between aquatic and terrestrial Phytophthora species along a climatic gradient are linked to functional traits
inferredcites →https://doi.org/10.1071/pc16032openalex-snapshot
Distribution and diversity of Phytophthora across Australia
inferredcites →https://doi.org/10.1073/pnas.1401884111openalex-snapshot
The Irish potato famine pathogen Phytophthora infestans originated in central Mexico rather than the Andes
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Environmental genes and genomes: understanding the differences and challenges in the approaches and software for their analyses
inferredcites →https://doi.org/10.1093/bioinformatics/btt593openalex-snapshot
PEAR: a fast and accurate Illumina Paired-End reAd mergeR
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Trimmomatic: a flexible trimmer for Illumina sequence data
inferredcites →https://doi.org/10.1093/femsec/fiy048openalex-snapshot
eDNA from roots: a robust tool for determining Phytophthora communities in natural ecosystems
inferredcites →https://doi.org/10.1093/sysbio/syq010openalex-snapshot
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
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First Report of Phytophthora europaea in Oak Forests in the Eastern and North-Central United States
inferredcites →https://doi.org/10.1094/pdis-07-16-0995-pdnopenalex-snapshot
First Report of Phytophthora cactorum Causing Root Rot of Lavender in China
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First Report of Phytophthora pseudosyringae Causing Basal Cankers on Horse Chestnut in Sweden
inferredcites →https://doi.org/10.1094/pdis.2000.84.1.4openalex-snapshot
Managing Port-Orford-Cedar and the Introduced Pathogen Phytophthora lateralis
inferredcites →https://doi.org/10.1094/phyto-03-17-0126-ropenalex-snapshot
Morphological and Genetic Analyses of the Invasive Forest Pathogen Phytophthora austrocedri Reveal that Two Clonal Lineages Colonized Britain and Argentina from a Common Ancestral Population
inferredcites →https://doi.org/10.1094/phyto.2003.93.6.695openalex-snapshot
Three Clonal Lineages of Phytophthora cinnamomi in Australia Revealed by Microsatellites
inferredcites →https://doi.org/10.1099/00221287-42-3-425openalex-snapshot
The Sterol Requirement of Phytophthora Cactorum
inferredcites →https://doi.org/10.1111/1365-2664.13068openalex-snapshot
Functional traits associated with the establishment of introduced Phytophthora spp. in Swedish forests
inferredcites →https://doi.org/10.1111/1574-6941.12132openalex-snapshot
Pyrosequencing of environmental soil samples reveals biodiversity of the Phytophthora resident community in chestnut forests
inferredcites →https://doi.org/10.1111/ecog.03904openalex-snapshot
Predictors of Phytophthora diversity and community composition in natural areas across diverse Australian ecoregions
inferredcites →https://doi.org/10.1111/efp.12037openalex-snapshot
Previously unrecorded low‐temperature P hytophthora species associated with Q uercus decline in a Mediterranean forest in eastern Spain
inferredcites →https://doi.org/10.1111/efp.12239openalex-snapshot
Widespread Phytophthora infestations in European nurseries put forest, semi‐natural and horticultural ecosystems at high risk of Phytophthora diseases
inferredcites →https://doi.org/10.1111/j.1365-294x.2009.04089.xopenalex-snapshot
Ancient isolation and independent evolution of the three clonal lineages of the exotic sudden oak death pathogen Phytophthora ramorum
inferredcites →https://doi.org/10.1111/j.1365-3059.2008.01886.xopenalex-snapshot
The biosecurity threat to the UK and global environment from international trade in plants
inferredcites →https://doi.org/10.1111/j.1439-0329.2008.00552.xopenalex-snapshot
Geographic distribution, disease symptoms and pathogenicity of Phytophthora nemorosa and Phytophthora pseudosyringae in California, USA
inferredcites →https://doi.org/10.1111/j.1755-0998.2011.03041.xopenalex-snapshot
DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer
inferredcites →https://doi.org/10.1111/nph.14776openalex-snapshot
PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives
inferredcites →https://doi.org/10.1111/ppa.12016openalex-snapshot
Annual and herbaceous perennial native A ustralian plant species are symptomless hosts of P hytophthora cinnamomi in the E ucalyptus marginata (jarrah) forest of W estern A ustralia
inferredcites →https://doi.org/10.1111/ppa.12067openalex-snapshot
Evidence of low levels of genetic diversity for the P hytophthora austrocedrae population in P atagonia, A rgentina
inferredcites →https://doi.org/10.1111/ppa.12253openalex-snapshot
Phytophthora austrocedrae emerges as a serious threat to juniper ( J uniperus communis ) in B ritain
inferredcites →https://doi.org/10.1111/ppa.12399openalex-snapshot
Dieback and mortality of Nothofagus in Britain: ecology, pathogenicity and sporulation potential of the causal agent Phytophthora pseudosyringae
inferredcites →https://doi.org/10.1111/ppa.12541openalex-snapshot
Metabarcoding and development of new real‐time specific assays reveal Phytophthora species diversity in holm oak forests in eastern Spain
inferredcites →https://doi.org/10.1111/ppa.12812openalex-snapshot
Genetic diversity of Phytophthora pluvialis , a pathogen of conifers, in New Zealand and the west coast of the United States of America
inferredcites →https://doi.org/10.1128/aem.00119-09openalex-snapshot
Zoosporic Tolerance to pH Stress and Its Implications for Phytophthora Species in Aquatic Ecosystems
inferredcites →https://doi.org/10.1128/msphere.00095-17openalex-snapshot
Phylogenomic Reconstruction of the Oomycete Phylogeny Derived from 37 Genomes
inferredcites →https://doi.org/10.1139/g98-060openalex-snapshot
Families of repeated DNA in the oomycete Phytophthora infestans and their distribution within the genus
inferredcites →https://doi.org/10.1146/annurev.phyto.38.1.541openalex-snapshot
New Frontiers in the Study of Dispersal and Spatial Analysis of Epidemics Caused by Species in the GenusPhytophthora
inferredcites →https://doi.org/10.1186/s12859-016-0976-yopenalex-snapshot
Illumina error profiles: resolving fine-scale variation in metagenomic sequencing data
inferredcites →https://doi.org/10.1186/s40490-014-0006-7openalex-snapshot
Pathogenicity of Phytophthora pluvialis to Pinus radiata and its relation with red needle cast disease in New Zealand
inferredcites →https://doi.org/10.1371/journal.pone.0119311openalex-snapshot
The Use of Genus-Specific Amplicon Pyrosequencing to Assess Phytophthora Species Diversity Using eDNA from Soil and Water in Northern Spain
inferredcites →https://doi.org/10.1371/journal.pone.0192502openalex-snapshot
Multiple origins of downy mildews and mito-nuclear discordance within the paraphyletic genus Phytophthora
inferredcites →https://doi.org/10.32614/cran.package.dharmaopenalex-snapshot
DHARMa: Residual Diagnostics for Hierarchical (Multi-Level / Mixed) Regression Models
inferredcites →https://doi.org/10.32614/cran.package.veganopenalex-snapshot
vegan: Community Ecology Package
inferredcites →https://doi.org/10.3767/003158509x442612openalex-snapshot
Re-evaluation of Phytophthora citricola isolates from multiple woody hosts in Europe and North America reveals a new species, Phytophthora plurivora sp. nov.
inferredcites →https://doi.org/10.3767/003158517x693615openalex-snapshot
Six new Phytophthora species from ITS Clade 7a including two sexually functional heterothallic hybrid species detected in natural ecosystems in Taiwan
inferredcites →https://doi.org/10.3852/10-058openalex-snapshot
Phytophthora pini Leonian resurrected to distinct species status
inferredcites →https://doi.org/10.4172/2157-7471.1000180openalex-snapshot
Phytophthora mississippiae sp. nov., a New Species Recovered from Irrigation Reservoirs at a Plant Nursery in Mississippi
inferredcites →https://doi.org/10.5197/j.2044-0588.2012.025.027openalex-snapshot
A new disease of Nothofagus in Britain caused by Phytophthora pseudosyringae
inferredcites →https://doi.org/10.5598/imafungus.2017.08.02.02openalex-snapshot
Multiple new cryptic pathogenic Phytophthora species from Fagaceae forests in Austria, Italy and Portugal
inferredcites →https://doi.org/10.5598/imafungus.2017.08.02.09openalex-snapshot
An expanded phylogeny for the genus Phytophthora
inferredcites →https://doi.org/10.5962/p.418468openalex-snapshot
Phytophthora nemorosa, a new species causing cankers and leaf blight of forest trees in California and Oregon, U.S.A
inferredcites →https://doi.org/10.7717/peerj.593openalex-snapshot
Swarm: robust and fast clustering method for amplicon-based studies

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← backgroundhttps://doi.org/10.5281/zenodo.3342957zenodo-depositor-declared
Metabarcoding reveals a high diversity of woody host-associated Phytophthora spp. in soils at public gardens and amenity woodlands in Britain
inferred← citeshttps://doi.org/10.1002/ece3.11308openalex-snapshot
Utility of decision tools for assessing plant health risks from management strategies in natural environments
inferred← citeshttps://doi.org/10.1002/edn3.300openalex-snapshot
Urban environments harbor greater oomycete and Phytophthora diversity, creating a bridgehead for potential new pathogens to natural ecosystems
inferred← citeshttps://doi.org/10.1002/edn3.529openalex-snapshot
Oomycete communities are influenced by land use and disease status in Christmas tree production in Southern Québec, Canada
inferred← citeshttps://doi.org/10.1002/pei3.70054openalex-snapshot
Impact of Phytophthora Disease on the Growth, Physiology and Ecosystem Services of Common Lime (Tilia × europaea) Street Trees
inferred← citeshttps://doi.org/10.1007/s00248-020-01563-0openalex-snapshot
Phytophthora Species Associated with Roots of Native and Non-native Trees in Natural and Managed Forests
inferred← citeshttps://doi.org/10.1007/s10658-023-02661-8openalex-snapshot
Sample volume affects the number of Phytophthora and Phytopythium species detected by soil baiting
inferred← citeshttps://doi.org/10.1007/s10658-024-02886-1openalex-snapshot
Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques
inferred← citeshttps://doi.org/10.1007/s11557-023-01892-7openalex-snapshot
Closing the gap between the number of Phytophthora species isolated through baiting a soil sample and the number revealed through metabarcoding
inferred← citeshttps://doi.org/10.1007/s11557-023-01925-1openalex-snapshot
Oomycete composition in Proteaceae orchards and natural stands on three continents
inferred← citeshttps://doi.org/10.1007/s11557-025-02039-6openalex-snapshot
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inferred← citeshttps://doi.org/10.1007/s11557-025-02091-2openalex-snapshot
Are environmental factors correlated with the diversity of Phytophthora in organically and conventionally managed citrus orchards in Sicily? Insights from metabarcoding and baiting
inferred← citeshttps://doi.org/10.1007/s40725-023-00188-zopenalex-snapshot
Continuous Replanting Could Degrade Soil Health in Short-Rotation Plantation Forestry
inferred← citeshttps://doi.org/10.1007/s40858-023-00630-4openalex-snapshot
Root and crown rot caused by oomycetes: an emerging threat to olive trees
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Co-infection of Trichothecium roseum (Pers.) Link and Penicillium italicum Wehmer on sour orange fruit and pH-mediated differences in pathogenicity
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inferred← citeshttps://doi.org/10.1016/j.foreco.2023.120806openalex-snapshot
Retention of post-harvest residues enhances soil fungal biodiversity in Eucalyptus plantations
inferred← citeshttps://doi.org/10.1016/j.foreco.2026.124292openalex-snapshot
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inferred← citeshttps://doi.org/10.1016/j.funbio.2021.01.009openalex-snapshot
Timing and abundance of sporangia production and zoospore release influences the recovery of different Phytophthora species by baiting
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inferred← citeshttps://doi.org/10.1080/14620316.2024.2404510openalex-snapshot
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inferred← citeshttps://doi.org/10.1093/g3journal/jkab282openalex-snapshot
Draft genome assemblies for tree pathogensPhytophthora pseudosyringaeandPhytophthora boehmeriae
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Full Issue PDF
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Susceptibility of Garden Plants to Phytophthora Root Rot
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inferred← citeshttps://doi.org/10.1111/efp.70012openalex-snapshot
Enhanced TaqMan qPCR Assay for Phytophthora cinnamomi Detection and Quantification
inferred← citeshttps://doi.org/10.1111/nph.17340openalex-snapshot
Humidity and high temperature are important for predicting fungal disease outbreaks worldwide
inferred← citeshttps://doi.org/10.1111/ppa.14044openalex-snapshot
The prevalence of Phytophthora in British plant nurseries; high‐risk hosts and substrates and opportunities to implement best practice
inferred← citeshttps://doi.org/10.1111/ppa.14049openalex-snapshot
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Guidelines for the reliable use of high throughput sequencing technologies to detect plant pathogens and pests
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inferred← citeshttps://doi.org/10.3390/f11111223openalex-snapshot
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inferred← citeshttps://doi.org/10.3390/f12020229openalex-snapshot
Diversity of Phytophthora Species Detected in Disturbed and Undisturbed British Soils Using High-Throughput Sequencing Targeting ITS rRNA and COI mtDNA Regions
inferred← citeshttps://doi.org/10.3390/f12121617openalex-snapshot
PHYTO-THREATS: Addressing Threats to UK Forests and Woodlands from Phytophthora; Identifying Risks of Spread in Trade and Methods for Mitigation
inferred← citeshttps://doi.org/10.3390/f15050735openalex-snapshot
Phytophthora Communities Associated with Agathis australis (kauri) in Te Wao Nui o Tiriwa/Waitākere Ranges, New Zealand
inferred← citeshttps://doi.org/10.3390/f16091419openalex-snapshot
Enhanced Detection of Phytophthora Species at P. pluvialis Outbreak Sites in Commercial Forests Across Britain
inferred← citeshttps://doi.org/10.3390/f17050566openalex-snapshot
Comparative Efficacy of × Hesperotropsis leylandii, Platycladus orientalis, and Juniperus communis Essential Oils Against Four Phytophthora Species Common in Nurseries and Forests
inferred← citeshttps://doi.org/10.3390/horticulturae12050561openalex-snapshot
Phytophthora pseudocryptogea Drives Dieback in Cycas revoluta: Comparative Pathogenicity of Three Phytophthora Species
inferred← citeshttps://doi.org/10.3390/horticulturae9060616openalex-snapshot
Phytophthora in Horticultural Nursery Green Waste—A Risk to Plant Health
inferred← citeshttps://doi.org/10.3390/jof11030187openalex-snapshot
The Heterogeneity of Ornamental Plants in Nurseries Increases the Chance of Finding New Hosts for Phytophthora
inferred← citeshttps://doi.org/10.3390/jof8040330openalex-snapshot
DNA Metabarcoding and Isolation by Baiting Complement Each Other in Revealing Phytophthora Diversity in Anthropized and Natural Ecosystems
inferred← citeshttps://doi.org/10.3390/jof8070737openalex-snapshot
New-Generation Sequencing Technology in Diagnosis of Fungal Plant Pathogens: A Dream Comes True?
inferred← citeshttps://doi.org/10.3390/jof8090980openalex-snapshot
Comparison of Primers for the Detection of Phytophthora (and Other Oomycetes) from Environmental Samples
inferred← citeshttps://doi.org/10.3390/microorganisms8050653openalex-snapshot
Comparative Genomic and Proteomic Analyses of Three Widespread Phytophthora Species: Phytophthora chlamydospora, Phytophthora gonapodyides and Phytophthora pseudosyringae
inferred← citeshttps://doi.org/10.3390/microorganisms8070973openalex-snapshot
Association of Phytophthora with Declining Vegetation in an Urban Forest Environment
inferred← citeshttps://doi.org/10.3390/plants12051197openalex-snapshot
Phytophthora pseudocryptogea, P. nicotianae and P. multivora Associated to Cycas revoluta: First Report Worldwide
inferred← citeshttps://doi.org/10.3390/plants9070826openalex-snapshot
First Report of Root Rot Caused by Phytophthora bilorbang on Olea europaea in Italy
inferred← citeshttps://doi.org/10.36253/phyto-17177openalex-snapshot
Phytophthora in forestry, natural environments, agriculture, and horticulture of the Mediterranean Basin and emerging Phytophthora threats from other regions
inferred← citeshttps://doi.org/10.3767/persoonia.2021.47.05openalex-snapshot
Current status of Phytophthora in Australia
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Current status of Phytophthora in Australia.
inferred← citeshttps://doi.org/10.56866/9789588360959.14openalex-snapshot
Enfermedades de los cultivares híbridos interespecíficos OxG
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Anticiper, comprendre et analyser les pathologies complexes et émergentes des chênes
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THAPBI PICT—a fast, cautious, and accurate metabarcoding analysis pipeline
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