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SPUD: A quantitative PCR assay for the detection of inhibitors in nucleic acid preparations

https://doi.org/10.1016/j.ab.2006.01.051
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What this work says

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inferred← citeshttps://doi.org/10.1111/j.1478-3231.2011.02732.xopenalex-snapshot
Mutant HrasG12V and KrasG12D have overlapping, but non‐identical effects on hepatocyte growth and transformation frequency in transgenic mice
inferred← citeshttps://doi.org/10.1111/jfd.13665openalex-snapshot
Detection of Kudoa thyrsites (Myxozoa) eDNA by real‐time and digital PCR from high seawater volumes
inferred← citeshttps://doi.org/10.1111/jfd.13912openalex-snapshot
Coinfection of channel catfish (Ictalurus punctatus) with virulent Aeromonas hydrophila and Flavobacterium covae exacerbates mortality
inferred← citeshttps://doi.org/10.1111/jwas.12608openalex-snapshot
Environmental factor(s) and animal vector(s) associated with atypical Aeromonas hydrophila abundance and dissemination among channel catfish ponds
inferred← citeshttps://doi.org/10.1111/jwas.12695openalex-snapshot
Monoculture of ♀ channel ( Ictalurus punctatus ) × ♂ blue ( I. furcatus ) hybrid catfish mitigates proliferative gill disease caused by Henneguya ictaluri (Cnidaria: Myxobolidae) in catfish aquaculture ponds
inferred← citeshttps://doi.org/10.1111/plb.12195openalex-snapshot
Cold storage to overcome dormancy affects the carbohydrate status and photosynthetic capacity of R hododendron simsii
inferred← citeshttps://doi.org/10.1111/rda.13193openalex-snapshot
Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production
inferred← citeshttps://doi.org/10.1128/aem.00677-11openalex-snapshot
Detection of Viable Cryptosporidium parvum in Soil by Reverse Transcription–Real-Time PCR Targeting hsp70 mRNA
inferred← citeshttps://doi.org/10.1128/aem.02188-15openalex-snapshot
Impacts of Long-Term Irrigation of Domestic Treated Wastewater on Soil Biogeochemistry and Bacterial Community Structure
inferred← citeshttps://doi.org/10.1128/aem.02872-16openalex-snapshot
Laboratory Surveillance of Polio and Other Enteroviruses in High-Risk Populations and Environmental Samples
inferred← citeshttps://doi.org/10.1128/jcm.00110-15openalex-snapshot
Aspergillus-Specific Lateral-Flow Device and Real-Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis
inferred← citeshttps://doi.org/10.1128/jcm.00131-12openalex-snapshot
Streptococcus pseudopneumoniae Identification by Pherotype: a Method To Assist Understanding of a Potentially Emerging or Overlooked Pathogen
inferred← citeshttps://doi.org/10.1128/mcb.01105-10openalex-snapshot
Lack of Protein 4.1G Causes Altered Expression and Localization of the Cell Adhesion Molecule Nectin-Like 4 in Testis and Can Cause Male Infertility
inferred← citeshttps://doi.org/10.1128/msphere.00821-24openalex-snapshot
“ Ca . Nitrosocosmicus” members are the dominant archaea associated with plant rhizospheres
inferred← citeshttps://doi.org/10.1134/s0006297914070128openalex-snapshot
MS2 phage ribonucleoproteins as exogenous internal control for RT-qPCR data normalization in gene expression study of developing rat brain
inferred← citeshttps://doi.org/10.1136/jclinpath-2014-202644openalex-snapshot
Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial
inferred← citeshttps://doi.org/10.1136/thoraxjnl-2012-201924openalex-snapshot
Changes in prevalence and load of airway bacteria using quantitative PCR in stable and exacerbated COPD
inferred← citeshttps://doi.org/10.1139/cjfas-2026-0030openalex-snapshot
Zebra mussels ( Dreissena polymorpha ) on the move: recent detections in Quebec and New Brunswick and implications for spread in Eastern Canada
inferred← citeshttps://doi.org/10.1152/ajpregu.90906.2008openalex-snapshot
Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles
inferred← citeshttps://doi.org/10.1152/japplphysiol.00679.2007openalex-snapshot
Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle
inferred← citeshttps://doi.org/10.11606/t.42.2014.tde-15122014-131506openalex-snapshot
Distribuição de fungos e fumonisinas e expressão dos genes FUM em grãos de milho transgênico da semeadura à colheita.
inferred← citeshttps://doi.org/10.1167/iovs.66.1.44openalex-snapshot
Posterior Limbal Mesenchymal Stromal Cells Promote Proliferation and Stemness of Transition Zone Cells: A Novel Insight Into Corneal Endothelial Rejuvenation
inferred← citeshttps://doi.org/10.1177/1040638713509378openalex-snapshot
DNA and RNA isolation from canine oncologic formalin-fixed, paraffin-embedded tissues for downstream “-omic” analyses
inferred← citeshttps://doi.org/10.1182/blood-2010-05-283879openalex-snapshot
Sequence variation at multiple loci influences red cell hemoglobin concentration
inferred← citeshttps://doi.org/10.1183/13993003.01225-2018openalex-snapshot
Airway microbiome in adult survivors of extremely preterm birth: the EPICure study
inferred← citeshttps://doi.org/10.1186/1471-2105-11-186openalex-snapshot
Shape based kinetic outlier detection in real-time PCR
inferred← citeshttps://doi.org/10.1186/1471-2105-9-326openalex-snapshot
A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
inferred← citeshttps://doi.org/10.1186/1471-2199-10-31openalex-snapshot
Reverse transcription-quantitative polymerase chain reaction: description of a RIN-based algorithm for accurate data normalization
inferred← citeshttps://doi.org/10.1186/1471-2199-10-62openalex-snapshot
Construction of an adult barnacle (Balanus amphitrite) cDNA library and selection of reference genes for quantitative RT-PCR studies
inferred← citeshttps://doi.org/10.1186/1471-2199-14-13openalex-snapshot
How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population
inferred← citeshttps://doi.org/10.1186/1753-6561-5-s6-p66openalex-snapshot
Quantitative PCR for etiologic diagnosis of methicillin-resistant Staphylococcus aureus pneumonia in intensive care unit
inferred← citeshttps://doi.org/10.1186/1756-0500-1-70openalex-snapshot
Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon
inferred← citeshttps://doi.org/10.1186/1756-0500-2-235openalex-snapshot
RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
inferred← citeshttps://doi.org/10.1186/1756-0500-5-668openalex-snapshot
DNA extract characterization process for microbial detection methods development and validation
inferred← citeshttps://doi.org/10.1186/gm481openalex-snapshot
Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies
inferred← citeshttps://doi.org/10.1186/s12862-015-0436-8openalex-snapshot
Rapid growth accelerates telomere attrition in a transgenic fish
inferred← citeshttps://doi.org/10.1186/s12870-015-0572-8openalex-snapshot
Sucrose accumulation in sweet sorghum stems occurs by apoplasmic phloem unloading and does not involve differential Sucrose transporter expression
inferred← citeshttps://doi.org/10.1186/s12885-015-1725-8openalex-snapshot
Development of novel real-time PCR methodology for quantification of COL11A1 mRNA variants and evaluation in breast cancer tissue specimens
inferred← citeshttps://doi.org/10.1186/s13071-022-05624-wopenalex-snapshot
High-throughput analysis of the transcriptional patterns of sexual genes in malaria
inferred← citeshttps://doi.org/10.1201/b14930-17openalex-snapshot
Assay Design for Real-Time qPCR
inferred← citeshttps://doi.org/10.1201/b14930-20openalex-snapshot
MIQE
inferred← citeshttps://doi.org/10.1261/rna.1877010openalex-snapshot
High-throughput quantification of splicing isoforms
inferred← citeshttps://doi.org/10.1292/jvms.16-0489openalex-snapshot
Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
inferred← citeshttps://doi.org/10.1371/journal.pgen.1007967openalex-snapshot
A synonymous germline variant in a gene encoding a cell adhesion molecule is associated with cutaneous mast cell tumour development in Labrador and Golden Retrievers
inferred← citeshttps://doi.org/10.1371/journal.pone.0006985openalex-snapshot
Implications of Storing Urinary DNA from Different Populations for Molecular Analyses
inferred← citeshttps://doi.org/10.1371/journal.pone.0040022openalex-snapshot
A MIQE-Compliant Real-Time PCR Assay for Aspergillus Detection
inferred← citeshttps://doi.org/10.1371/journal.pone.0063968openalex-snapshot
Quantitative Detection of Borrelia burgdorferi sensu lato in Erythema Migrans Skin Lesions Using Internally Controlled Duplex Real Time PCR
inferred← citeshttps://doi.org/10.1371/journal.pone.0068481openalex-snapshot
Accurate and Precise DNA Quantification in the Presence of Different Amplification Efficiencies Using an Improved Cy0 Method
inferred← citeshttps://doi.org/10.1371/journal.pone.0088266openalex-snapshot
Five Years MIQE Guidelines: The Case of the Arabian Countries
inferred← citeshttps://doi.org/10.1371/journal.pone.0190041openalex-snapshot
Capacity of rTth polymerase to detect RNA in the presence of various inhibitors
inferred← citeshttps://doi.org/10.1373/clinchem.2008.112797openalex-snapshot
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
inferred← citeshttps://doi.org/10.1373/clinchem.2013.206375openalex-snapshot
The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments
inferred← citeshttps://doi.org/10.1534/genetics.109.103945openalex-snapshot
The Role of MITF Phosphorylation Sites During Coat Color and Eye Development in Mice Analyzed by Bacterial Artificial Chromosome Transgene Rescue
inferred← citeshttps://doi.org/10.1586/14737159.9.2.187openalex-snapshot
Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
inferred← citeshttps://doi.org/10.1590/1678-4499.20230270openalex-snapshot
Morphological screening and expression of droughtrelated genes P5SC1 and DREB1A in water-stressed pearl millet (Pennisetum glaucum) at the pre-fruiting stage
inferred← citeshttps://doi.org/10.20315/asetl.100.3openalex-snapshot
Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
inferred← citeshttps://doi.org/10.21203/rs.3.rs-75000/v1openalex-snapshot
CoV2-ID, a MIQE-compliant sub-20-minute 5-plex RT-PCR assay targeting SARS-CoV-2 for the diagnosis of COVID-19
inferred← citeshttps://doi.org/10.21273/jashs.134.6.589openalex-snapshot
Relationship between the Hormonal Balance and the Regulation of Iron Deficiency Stress Responses in Cucumber
inferred← citeshttps://doi.org/10.2144/000113244openalex-snapshot
A Quantitative Approach to Detect and Overcome PCR Inhibition in Ancient DNA Extracts
inferred← citeshttps://doi.org/10.2166/wh.2018.222openalex-snapshot
Campylobacter in recreational lake water in southern Quebec, Canada: presence, concentration, and association with precipitation and ruminant farm proximity
inferred← citeshttps://doi.org/10.21775/9781912530243.12openalex-snapshot
MIQE: Guidelines for the Design and Publication of a Reliable Real-time PCR Assay
inferred← citeshttps://doi.org/10.21775/cimb.012.129openalex-snapshot
SPUD qPCR Assay Confirms PREXCEL-Q Software's Ability to Avoid qPCR Inhibition
inferred← citeshttps://doi.org/10.2217/17520363.2.3.201openalex-snapshot
Molecular Medicine, Gene-Expression Profiling and Molecular Diagnostics: Putting the Cart Before the Horse
inferred← citeshttps://doi.org/10.2478/v10014-011-0006-8openalex-snapshot
Total RNA extraction method and Prunus species influence the detection of Plum pox potyvirus by real-time RT-PCR
inferred← citeshttps://doi.org/10.29326/2304-196x-2023-12-3-197-207openalex-snapshot
Current approaches to development of real-time qPCR test-kits
inferred← citeshttps://doi.org/10.3103/s0891416809030033openalex-snapshot
Development of noncompetitive exogenous internal amplification control for real-time PCR based on UFA method
inferred← citeshttps://doi.org/10.3133/sir20125087openalex-snapshot
Interlaboratory comparison of three microbial source tracking quantitative polymerase chain reaction (qPCR) assays from fecal-source and environmental samples
inferred← citeshttps://doi.org/10.3168/jds.2012-5511openalex-snapshot
Use of Prevotella bryantii 25A and a commercial probiotic during subacute acidosis challenge in midlactation dairy cows
inferred← citeshttps://doi.org/10.3354/ame01817openalex-snapshot
Aerobic and anaerobic ammonium oxidizers in the Cariaco Basin: distributions of major taxa and nitrogen species across the redoxcline
inferred← citeshttps://doi.org/10.3354/dao02579openalex-snapshot
Development and validation of a quantitative PCR assay for Ichthyophonus spp.
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