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Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

https://doi.org/10.1021/acs.nanolett.1c00715

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Digital CRISPR-based method for the rapid detection and absolute quantification of nucleic acids

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Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay

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Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

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Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

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Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.

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Ultrasensitive Coincidence Fluorescence Detection of Single DNA Molecules

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Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

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Molecular Diagnosis of COVID-19: Challenges and Research Needs

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Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

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An Ultralocalized Cas13a Assay Enables Universal and Nucleic Acid Amplification-Free Single-Molecule RNA Diagnostics

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CRISPR-Cas12a-Assisted Multicolor Biosensor for Semiquantitative Point-of-Use Testing of the Nopaline Synthase Terminator in Genetically Modified Crops by Unaided Eyes

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HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

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CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA

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PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage

inferredcites →https://doi.org/10.1093/nar/gng093openalex-snapshot

Mathematics of quantitative kinetic PCR and the application of standard curves

inferredcites →https://doi.org/10.1101/gr.6.10.986openalex-snapshot

Real time quantitative PCR.

inferredcites →https://doi.org/10.1111/j.1365-2141.2007.06620.xopenalex-snapshot

Angioimmunoblastic T‐cell lymphoma: histological progression associates with EBV and HHV6B viral load

inferredcites →https://doi.org/10.1111/j.1749-6632.2001.tb03865.xopenalex-snapshot

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Incoming claims: where this work appears in other papers’ reference lists and arguments.

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inferred← citeshttps://doi.org/10.1016/j.tifs.2024.104349openalex-snapshot

Detection and absolute quantification biosensing tools for food authentication: CRISPR/Cas, digital CRISPR and beyond

inferred← citeshttps://doi.org/10.1016/j.trac.2022.116539openalex-snapshot

Molecular Reporters for Crispr/Cas: From Design Principles to Engineering for Bioanalytical and Diagnostic Applications

inferred← citeshttps://doi.org/10.1016/j.trac.2022.116812openalex-snapshot

Recent progress in CRISPR-based microfluidic assays and applications

inferred← citeshttps://doi.org/10.1016/j.trac.2022.116871openalex-snapshot

Recent advancements in nucleic acid detection with microfluidic chip for molecular diagnostics

inferred← citeshttps://doi.org/10.1016/j.trac.2022.116897openalex-snapshot

Droplet-based digital PCR (ddPCR) and its applications

inferred← citeshttps://doi.org/10.1016/j.trac.2023.116917openalex-snapshot

Digital CRISPR systems for the next generation of nucleic acid quantification

inferred← citeshttps://doi.org/10.1016/j.trac.2023.116931openalex-snapshot

Nucleic acid-assisted CRISPR-Cas systems for advanced biosensing and bioimaging

inferred← citeshttps://doi.org/10.1016/j.trac.2023.117035openalex-snapshot

Single microentity analysis-based ultrasensitive bioassays: Recent advances, applications, and perspectives

inferred← citeshttps://doi.org/10.1016/j.trac.2024.118128openalex-snapshot

CRISPR-enabled sensors for rapid monitoring of environmental contaminants

inferred← citeshttps://doi.org/10.1016/j.trac.2025.118268openalex-snapshot

Nucleic acid-based reference materials: A “ruler” for precision molecular diagnostics

inferred← citeshttps://doi.org/10.1016/j.trac.2025.118296openalex-snapshot

Engineering CRISPR/Cas12-based biosensors: Recent advances and future perspectives

inferred← citeshttps://doi.org/10.1016/j.trac.2025.118590openalex-snapshot

CRISPR-cas diagnostics (CRISPR-Dx) of viral pathogens in low and limited resource areas: Current state and future directions

inferred← citeshttps://doi.org/10.1016/j.trac.2026.118971openalex-snapshot

Discretization mechanisms in digital protein detection: Technologies, applications, and future opportunities

inferred← citeshttps://doi.org/10.1016/j.ymeth.2022.06.002openalex-snapshot

CRISPR-Cas, Argonaute proteins and the emerging landscape of amplification-free diagnostics

inferred← citeshttps://doi.org/10.1017/qrd.2022.7openalex-snapshot

Inconsistent treatments of the kinetics of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) impair assessment of its diagnostic potential

inferred← citeshttps://doi.org/10.1017/qrd.2022.7.pr1openalex-snapshot

Review: Inconsistent Treatments of CRISPR Kinetics Impair Assessment of Its Diagnostic Potential — R0/PR1

inferred← citeshttps://doi.org/10.1017/qrd.2022.7.pr2openalex-snapshot

Review: Inconsistent Treatments of CRISPR Kinetics Impair Assessment of Its Diagnostic Potential — R0/PR2

inferred← citeshttps://doi.org/10.1017/qrd.2022.7.pr3openalex-snapshot

Decision: Inconsistent Treatments of CRISPR Kinetics Impair Assessment of Its Diagnostic Potential — R0/PR3

inferred← citeshttps://doi.org/10.1017/qrd.2022.7.pr4openalex-snapshot

Decision: Inconsistent Treatments of CRISPR Kinetics Impair Assessment of Its Diagnostic Potential — R1/PR4

inferred← citeshttps://doi.org/10.1017/qrd.2022.7.pr5openalex-snapshot

Decision: Inconsistent Treatments of CRISPR Kinetics Impair Assessment of Its Diagnostic Potential — R2/PR5

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c00427openalex-snapshot

Agarose-Droplet-Based Digital LAMP Assay for Counting Virus DNA in Single-Particle ICP-MS

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c00615openalex-snapshot

Droplet Microfluidics for High-Resolution Virology

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c01193openalex-snapshot

Photoactivatable CRISPR/Cas12a Strategy for One-Pot DETECTR Molecular Diagnosis

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c01670openalex-snapshot

Enzyme Kinetics and Detector Sensitivity Determine Limits of Detection of Amplification-Free CRISPR-Cas12 and CRISPR-Cas13 Diagnostics

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c02283openalex-snapshot

Amplification-Free, Single-Microbead-Based Cas12a Assay for One-Step DNA Detection at the Single-Molecule Level

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c03590openalex-snapshot

PddCas: A Polydisperse Droplet Digital CRISPR/Cas-Based Assay for the Rapid and Ultrasensitive Amplification-Free Detection of Viral DNA/RNA

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c03945openalex-snapshot

Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c04555openalex-snapshot

Signal Amplification by the trans -Cleavage Activity of CRISPR-Cas Systems: Kinetics and Performance

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c04701openalex-snapshot

Rapid In Situ Hydrogel LAMP for On-Site Large-Scale Parallel Single-Cell HPV Detection

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c04713openalex-snapshot

Coupling CRISPR/Cas12a and Recombinase Polymerase Amplification on a Stand-Alone Microfluidics Platform for Fast and Parallel Nucleic Acid Detection

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c05320openalex-snapshot

Integrating CRISPR-Cas12a into a Microfluidic Dual-Droplet Device Enables Simultaneous Detection of HPV16 and HPV18

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c05767openalex-snapshot

CRISPR-Cas-Driven Single Micromotor (Cas-DSM) Enables Direct Detection of Nucleic Acid Biomarkers at the Single-Molecule Level

inferred← citeshttps://doi.org/10.1021/acs.analchem.3c02983openalex-snapshot

Digital-like Enzyme Inhibition Mechanism-Based Strategy for the Digital Sensing of Heparin-Specific Biomarkers

inferred← citeshttps://doi.org/10.1021/acs.analchem.3c04363openalex-snapshot

CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

inferred← citeshttps://doi.org/10.1021/acs.analchem.3c04426openalex-snapshot

Mesophilic Argonaute-Mediated Polydisperse Droplet Biosensor for Amplification-Free, One-Pot, and Multiplexed Nucleic Acid Detection Using Deep Learning

inferred← citeshttps://doi.org/10.1021/acs.analchem.4c02204openalex-snapshot

A Propidium Monoazide-Assisted Digital CRISPR/Cas12a Assay for Selective Detection of Live Bacteria in Sample

inferred← citeshttps://doi.org/10.1021/acs.analchem.5c01060openalex-snapshot

Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics

inferred← citeshttps://doi.org/10.1021/acs.analchem.5c08178openalex-snapshot

SIMPLE-CRISPR: A Sample-to-Result Platform for Point-of-Care Detection of Nucleic Acids via a Functionalized Magnetic-Bead-Based CRISPR Assay

inferred← citeshttps://doi.org/10.1021/acs.analchem.6c00173openalex-snapshot

A Disposable CRISPR-Nanozyme Electrochemical Biosensor for Rapid and Sensitive Detection of Breast Cancer Circulating Tumor DNA

inferred← citeshttps://doi.org/10.1021/acs.analchem.6c00936openalex-snapshot

Single-Molecule Characterization of CRISPR–Cas12a for Amplification-Free Genetic Testing

inferred← citeshttps://doi.org/10.1021/acs.analchem.6c01288openalex-snapshot

Digital Hydrogel Fluorescent-Enhancing Microspheres via CRISPR/Cas12a for Amplification-Free Relative Quantification of Nucleic Acids

inferred← citeshttps://doi.org/10.1021/acs.analchem.6c03578openalex-snapshot

Cascade-Amplified Microfluidic Detection of Tumor Biomarkers via Metal-DNA Nanospheres Activating CRISPR-Cas12a Assay

inferred← citeshttps://doi.org/10.1021/acs.jafc.1c08262openalex-snapshot

Ultrasensitive CRISPR/Cas12a-Driven SERS Biosensor for On-Site Nucleic Acid Detection and Its Application to Milk Authenticity Testing

inferred← citeshttps://doi.org/10.1021/acs.jafc.5c04162openalex-snapshot

CRISPR/Cas-Based Biosensing Strategies for Non-Nucleic Acid Contaminants in Food Safety: Status, Challenges, and Perspectives

inferred← citeshttps://doi.org/10.1021/acs.nanolett.4c06490openalex-snapshot

Single-Molecule Amplification-Free Detection of Nucleic Acid Biomarkers from Body Fluids via an Optical Microfiber with a Nanointerface

inferred← citeshttps://doi.org/10.1021/acs.nanolett.6c00238openalex-snapshot

Single Microbead Floating/Sinking-Enabled Digital Biosensing

inferred← citeshttps://doi.org/10.1021/acsami.3c17039openalex-snapshot

Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Multifunctional CRISPR-Cas12a

inferred← citeshttps://doi.org/10.1021/acsnano.2c10143openalex-snapshot

An Integrated Amplification-Free Digital CRISPR/Cas-Assisted Assay for Single Molecule Detection of RNA

inferred← citeshttps://doi.org/10.1021/acsnano.3c01917openalex-snapshot

STAMP-Based Digital CRISPR-Cas13a for Amplification-Free Quantification of HIV-1 Plasma Viral Loads

inferred← citeshttps://doi.org/10.1021/acsnano.4c10823openalex-snapshot

Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

inferred← citeshttps://doi.org/10.1021/acsnano.4c13557openalex-snapshot

Concurrent Detection of Protein and miRNA at the Single Extracellular Vesicle Level Using a Digital Dual CRISPR-Cas Assay

inferred← citeshttps://doi.org/10.1021/acsnano.5c03474openalex-snapshot

CRISPR Digital Sensing: From Micronano-Collaborative Chip to Biomolecular Detection

inferred← citeshttps://doi.org/10.1021/acsomega.4c03413openalex-snapshot

Rapid and Amplification-free Nucleic Acid Detection with DNA Substrate-Mediated Autocatalysis of CRISPR/Cas12a

inferred← citeshttps://doi.org/10.1021/acssensors.2c00024openalex-snapshot

Figure of Merit for CRISPR-Based Nucleic Acid-Sensing Systems: Improvement Strategies and Performance Comparison

inferred← citeshttps://doi.org/10.1021/acssensors.2c01200openalex-snapshot

Tandem Cas13a/crRNA-Mediated CRISPR-FET Biosensor: A One-for-All Check Station for Virus without Amplification

inferred← citeshttps://doi.org/10.1021/acssensors.2c01955openalex-snapshot

A Localized CRISPR Assay that Detects Short Nucleic Acid Fragments in Unamplified Genetically Modified Samples

inferred← citeshttps://doi.org/10.1021/acssensors.2c02495openalex-snapshot

CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination

inferred← citeshttps://doi.org/10.1021/acssensors.3c00587openalex-snapshot

Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device

inferred← citeshttps://doi.org/10.1021/acssensors.3c01463openalex-snapshot

CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives

inferred← citeshttps://doi.org/10.1021/acssensors.4c00652openalex-snapshot

FRETting about CRISPR-Cas Assays: Dual-Channel Reporting Lowers Detection Limits and Times-to-Result

inferred← citeshttps://doi.org/10.1021/acssensors.4c01248openalex-snapshot

DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA

inferred← citeshttps://doi.org/10.1021/acssynbio.4c00816openalex-snapshot

Building the Future of Clinical Diagnostics: An Analysis of Potential Benefits and Current Barriers in CRISPR/Cas Diagnostics

inferred← citeshttps://doi.org/10.1021/cbe.3c00112openalex-snapshot

Exploiting the Specificity of CRISPR/Cas System for Nucleic Acids Amplification-Free Disease Diagnostics in the Point-of-Care

inferred← citeshttps://doi.org/10.1038/s41467-022-32371-4openalex-snapshot

A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

inferred← citeshttps://doi.org/10.1038/s41467-022-34086-yopenalex-snapshot

Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification

inferred← citeshttps://doi.org/10.1038/s41467-024-47518-8openalex-snapshot

Ultrasensitive single-step CRISPR detection of monkeypox virus in minutes with a vest-pocket diagnostic device

inferred← citeshttps://doi.org/10.1038/s41467-026-68476-3openalex-snapshot

Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection

inferred← citeshttps://doi.org/10.1038/s41551-025-01597-0openalex-snapshot

Digital CRISPR-based diagnostics for quantification of Candida auris and resistance mutations

inferred← citeshttps://doi.org/10.1039/d1sc05558eopenalex-snapshot

PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing

inferred← citeshttps://doi.org/10.1039/d2cc01300bopenalex-snapshot

Simultaneous detection of CaMV35S and T-nos utilizing CRISPR/Cas12a and Cas13a with multiplex-PCR (MPT-Cas12a/13a)

inferred← citeshttps://doi.org/10.1039/d2cs00594hopenalex-snapshot

Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives

inferred← citeshttps://doi.org/10.1039/d2lc00223jopenalex-snapshot

Enzyme-based digital bioassay technology – key strategies and future perspectives

inferred← citeshttps://doi.org/10.1039/d2lc00284aopenalex-snapshot

Parallel multistep digital analysis SlipChip demonstrated with the quantification of nucleic acid by digital LAMP-CRISPR

inferred← citeshttps://doi.org/10.1039/d3ay01795hopenalex-snapshot

The development of droplet-based microfluidic virus detection technology for human infectious diseases

inferred← citeshttps://doi.org/10.1039/d3cc03984fopenalex-snapshot

Rapid and sensitive point-of-care PTS-CRISPR assay for food safety monitoring of aflatoxin B1

inferred← citeshttps://doi.org/10.1039/d4lc00340copenalex-snapshot

CRISPR for companion diagnostics in low-resource settings

inferred← citeshttps://doi.org/10.1039/d5an01178gopenalex-snapshot

A photonic crystal sensing array based on a tandem CRISPR/Cas13a system for ultra-sensitive and high-throughput detection of the CVA6 virus

inferred← citeshttps://doi.org/10.1039/d5cs00023hopenalex-snapshot

Opto-digital molecular analytics

inferred← citeshttps://doi.org/10.1039/d5lc00340gopenalex-snapshot

High-throughput monoclonal antibody screening from immunized rabbits via droplet microfluidics

inferred← citeshttps://doi.org/10.1039/d6cc04236hopenalex-snapshot

Confinement-enabled nanobiosensing: from chemical signal amplification to programmable single-molecule diagnostics

inferred← citeshttps://doi.org/10.1080/07388551.2024.2399560openalex-snapshot

Amplification-free nucleic acids detection with next-generation CRISPR/dx systems

inferred← citeshttps://doi.org/10.1089/crispr.2023.0023openalex-snapshot

Advances in Cas12a-Based Amplification-Free Nucleic Acid Detection

inferred← citeshttps://doi.org/10.1097/mog.0000000000000895openalex-snapshot

Point of care diagnostics for Cryptosporidium: new and emerging technologies

inferred← citeshttps://doi.org/10.1101/2021.07.21.21260653openalex-snapshot

A Combinatorial Approach towards Adaptability of 22 Functional Cas12a Orthologs for Nucleic Acid Detection in Clinical Samples

inferred← citeshttps://doi.org/10.1101/2021.08.02.21261509openalex-snapshot

Sensitive and multiplexed RNA detection with Cas13 droplets and kinetic barcoding

inferred← citeshttps://doi.org/10.1101/2021.08.18.21262201openalex-snapshot

Microfluidics-Enabled Digital Isothermal Cas13a Assay

inferred← citeshttps://doi.org/10.1101/2022.01.31.478567openalex-snapshot

Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

inferred← citeshttps://doi.org/10.1101/2022.10.13.512138openalex-snapshot

STAMP-Based Digital CRISPR-Cas13a (STAMP-dCRISPR) for Amplification-Free Quantification of HIV-1 Plasma Viral Load

inferred← citeshttps://doi.org/10.1109/jsen.2025.3572615openalex-snapshot

Advances in One-Tube Biomarker Detection Technology Based on CRISPR/Cas Signal Amplification: A Review

inferred← citeshttps://doi.org/10.1128/spectrum.01017-21openalex-snapshot

CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern

inferred← citeshttps://doi.org/10.1128/spectrum.03645-23openalex-snapshot

A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants

inferred← citeshttps://doi.org/10.1146/annurev-anchem-090822-014725openalex-snapshot

CRISPR-Based Biosensing Strategies: Technical Development and Application Prospects

inferred← citeshttps://doi.org/10.1146/annurev-bioeng-081723-013033openalex-snapshot

CRISPR/Cas Technology: The Unique Synthetic Biology Genome-Editing Tool Shifting the Paradigm in Viral Diagnostics, Defense, and Therapeutics

inferred← citeshttps://doi.org/10.15421/0225072openalex-snapshot

The identification of Cryptosporidium species in stool samples using alternative diagnostic methods

inferred← citeshttps://doi.org/10.2139/ssrn.5354604openalex-snapshot

Leveraging High Temperature for Enhanced and Faster Detection of Sars-Cov-2 Variants with Crispr-Ascas12a

inferred← citeshttps://doi.org/10.26717/bjstr.2022.46.007422openalex-snapshot

"Preamplification and Amplification-Free Detection of SARS-CoV-2 based on CRISPR/CAS SYSTEM"

inferred← citeshttps://doi.org/10.3389/fbioe.2022.851712openalex-snapshot

Powerful CRISPR-Based Biosensing Techniques and Their Integration With Microfluidic Platforms

inferred← citeshttps://doi.org/10.3389/fbioe.2022.986233openalex-snapshot

Research progress of CRISPR-based biosensors and bioassays for molecular diagnosis

inferred← citeshttps://doi.org/10.3389/fimmu.2023.1172035openalex-snapshot

Outlook for CRISPR-based tuberculosis assays now in their infancy

inferred← citeshttps://doi.org/10.3389/fmed.2025.1596579openalex-snapshot

Advancing the fight against tuberculosis: integrating innovation and public health in diagnosis, treatment, vaccine development, and implementation science

inferred← citeshttps://doi.org/10.3389/fmicb.2021.751408openalex-snapshot

Recent Improvements in CRISPR-Based Amplification-Free Pathogen Detection

inferred← citeshttps://doi.org/10.3389/fmicb.2021.766464openalex-snapshot

Definition of CRISPR Cas12a Trans-Cleavage Units to Facilitate CRISPR Diagnostics

inferred← citeshttps://doi.org/10.3389/fmicb.2025.1509890openalex-snapshot

CRISPR-Cas for hepatitis virus: a systematic review and meta-analysis of diagnostic test accuracy studies

inferred← citeshttps://doi.org/10.3390/bios12090673openalex-snapshot

Recent Advances in Digital Biosensing Technology

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