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Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics

https://doi.org/10.1101/2022.01.31.478567
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/adma.201905311openalex-snapshot
CRISPR/Cas13a‐Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification‐Free miRNA Diagnostics
inferredcites →https://doi.org/10.1002/advs.201903661openalex-snapshot
CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection
inferredcites →https://doi.org/10.1016/j.ab.2007.07.008openalex-snapshot
Rapid determination of enzyme kinetics from fluorescence: Overcoming the inner filter effect
inferredcites →https://doi.org/10.1016/j.cell.2020.12.001openalex-snapshot
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
inferredcites →https://doi.org/10.1016/j.celrep.2019.02.094openalex-snapshot
High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage
inferredcites →https://doi.org/10.1016/j.celrep.2021.108865openalex-snapshot
High-resolution structure of cas13b and biochemical characterization of RNA targeting and cleavage
inferredcites →https://doi.org/10.1016/j.isci.2021.102996openalex-snapshot
Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics
inferredcites →https://doi.org/10.1016/j.jpba.2021.114268openalex-snapshot
A label-free impedance biosensing assay based on CRISPR/Cas12a collateral activity for bacterial DNA detection
inferredcites →https://doi.org/10.1021/acs.analchem.1c00525openalex-snapshot
CRISPR Enzyme Kinetics for Molecular Diagnostics
inferredcites →https://doi.org/10.1021/acs.analchem.9b00073openalex-snapshot
High-Fidelity and Rapid Quantification of miRNA Combining crRNA Programmability and CRISPR/Cas13a trans-Cleavage Activity
inferredcites →https://doi.org/10.1021/acs.nanolett.1c00715openalex-snapshot
Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level
inferredcites →https://doi.org/10.1038/s41467-020-18615-1openalex-snapshot
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
inferredcites →https://doi.org/10.1038/s41467-021-21996-6openalex-snapshot
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing
inferredcites →https://doi.org/10.1038/s41551-019-0371-xopenalex-snapshot
Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor
inferredcites →https://doi.org/10.1038/s41551-021-00760-7openalex-snapshot
CRISPR-based diagnostics
inferredcites →https://doi.org/10.1038/s41587-020-0513-4openalex-snapshot
CRISPR–Cas12-based detection of SARS-CoV-2
inferredcites →https://doi.org/10.1038/s41589-021-00842-2openalex-snapshot
Accelerated RNA detection using tandem CRISPR nucleases
inferredcites →https://doi.org/10.1038/s41596-019-0210-2openalex-snapshot
SHERLOCK: nucleic acid detection with CRISPR nucleases
inferredcites →https://doi.org/10.1038/s42003-021-02001-8openalex-snapshot
Amplification-free RNA detection with CRISPR–Cas13
inferredcites →https://doi.org/10.1039/d1sc02197dopenalex-snapshot
An amplification-free ultra-sensitive electrochemical CRISPR/Cas biosensor for drug-resistant bacteria detection
inferredcites →https://doi.org/10.1056/nejmc2026172openalex-snapshot
Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing
inferredcites →https://doi.org/10.1073/pnas.2010254117openalex-snapshot
Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2
inferredcites →https://doi.org/10.1126/science.aaq0179openalex-snapshot
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
inferredcites →https://doi.org/10.1126/science.aar6245openalex-snapshot
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
inferredcites →https://doi.org/10.7554/elife.52760openalex-snapshot
The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1021/acs.analchem.2c03338openalex-snapshot
Detection and Discrimination of Single Nucleotide Polymorphisms by Quantification of CRISPR-Cas Catalytic Efficiency
inferred← citeshttps://doi.org/10.1038/s42003-022-03433-6openalex-snapshot
Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis
inferred← citeshttps://doi.org/10.1038/s42003-026-10127-wopenalex-snapshot
Statistical crystallography reveals an allosteric network in SARS-CoV-2 Mpro
inferred← citeshttps://doi.org/10.1101/2022.06.13.495973openalex-snapshot
Cas12a2 elicits abortive infection via RNA-triggered destruction of double-stranded DNA
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