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Optimization of membrane protein overexpression and purification using GFP fusions

https://doi.org/10.1038/nmeth0406-303
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/pro.5560070420openalex-snapshot
Genome‐wide analysis of integral membrane proteins from eubacterial, archaean, and eukaryotic organisms
inferredcites →https://doi.org/10.1016/j.jmb.2005.07.053openalex-snapshot
Experimentally Constrained Topology Models for 51,208 Bacterial Inner Membrane Proteins
inferredcites →https://doi.org/10.1016/s0005-2736(02)00709-5openalex-snapshot
Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies
inferredcites →https://doi.org/10.1016/s0014-5793(01)02980-5openalex-snapshot
Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli
inferredcites →https://doi.org/10.1016/s0168-1656(02)00161-xopenalex-snapshot
Green fluorescent protein as a reporter of human μ-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris
inferredcites →https://doi.org/10.1038/nbt1172openalex-snapshot
Engineering and characterization of a superfolder green fluorescent protein
inferredcites →https://doi.org/10.1073/pnas.052018199openalex-snapshot
Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
inferredcites →https://doi.org/10.1110/ps.03553804openalex-snapshot
Experimentally based topology models for E. coli inner membrane proteins
inferredcites →https://doi.org/10.1110/ps.051466205openalex-snapshot
A scalable, GFP‐based pipeline for membrane protein overexpression screening and purification
inferredcites →https://doi.org/10.1126/science.1087619openalex-snapshot
Structure and Mechanism of the Glycerol-3-Phosphate Transporter from Escherichia coli
inferredcites →https://doi.org/10.1126/science.1109730openalex-snapshot
Global Topology Analysis of the Escherichia coli Inner Membrane Proteome
inferredcites →https://doi.org/10.1128/jb.182.14.4068-4076.2000openalex-snapshot
Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli
inferredcites →https://doi.org/10.1186/1475-2859-4-3openalex-snapshot
Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly
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Membrane Protein Production in Escherichia coli: Protocols and Rules
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Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach
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Split GFP Complementation as Reporter of Membrane Protein Expression and Stability in E. coli: A Tool to Engineer Stability in a LAT Transporter
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Optimization of Membrane Protein Production Using Titratable Strains of E. coli
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A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production
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Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
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Large-Scale Production of Membrane Proteins in Saccharomyces cerevisiae: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
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Green Fluorescent Protein as a protein localization and topological reporter in mycobacteria
inferred← citeshttps://doi.org/10.1016/j.virol.2007.06.006openalex-snapshot
Membrane insertion and topology of the p7B movement protein of Melon Necrotic Spot Virus (MNSV)
inferred← citeshttps://doi.org/10.1016/j.virol.2012.10.022openalex-snapshot
Reduced expression of Autographa californica nucleopolyhedrovirus ORF34, an essential gene, enhances heterologous gene expression
inferred← citeshttps://doi.org/10.1016/j.ymben.2012.05.001openalex-snapshot
Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli
inferred← citeshttps://doi.org/10.1016/j.ymeth.2018.04.009openalex-snapshot
Microbial expression systems for membrane proteins
inferred← citeshttps://doi.org/10.1016/j.ymeth.2018.05.014openalex-snapshot
The fine art of integral membrane protein crystallisation
inferred← citeshttps://doi.org/10.1016/s1063-5823(09)63011-8openalex-snapshot
Chapter 11 Bacterial Membrane Proteins
inferred← citeshttps://doi.org/10.1021/acs.biochem.5b00633openalex-snapshot
Structural Insights into the Distinct Binding Mode of Cyclic Di-AMP with SaCpaA_RCK
inferred← citeshttps://doi.org/10.1021/acs.biochem.8b01070openalex-snapshot
An Improved Strategy for Fluorescent Tagging of Membrane Proteins for Overexpression and Purification in Mammalian Cells
inferred← citeshttps://doi.org/10.1021/acs.biomac.6c00782openalex-snapshot
Nanodisc-Forming Polymers for the Extraction of Membrane Proteins
inferred← citeshttps://doi.org/10.1021/acs.cgd.7b01678openalex-snapshot
In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates
inferred← citeshttps://doi.org/10.1021/acs.jcim.3c00917openalex-snapshot
Combined Computational–Biochemical Approach Offers an Accelerated Path to Membrane Protein Solubilization
inferred← citeshttps://doi.org/10.1021/acsami.9b09071openalex-snapshot
Novel Synthesis Strategy for Biocatalyst: Fast Purification and Immobilization of His- and ELP-Tagged Enzyme from Fermentation Broth
inferred← citeshttps://doi.org/10.1021/acschembio.1c00100openalex-snapshot
Chemogenetic Tags with Probe Exchange for Live-Cell Fluorescence Microscopy
inferred← citeshttps://doi.org/10.1021/acssynbio.1c00598openalex-snapshot
Second-Generation Escherichia coli SuptoxR Strains for High-Level Recombinant Membrane Protein Production
inferred← citeshttps://doi.org/10.1021/acssynbio.2c00358openalex-snapshot
Silencing Transcription from an Influenza Reverse Genetics Plasmid in E. coli Enhances Gene Stability
inferred← citeshttps://doi.org/10.1021/acssynbio.6b00174openalex-snapshot
Development of Escherichia coli Strains That Withstand Membrane Protein-Induced Toxicity and Achieve High-Level Recombinant Membrane Protein Production
inferred← citeshttps://doi.org/10.1021/acssynbio.6b00321openalex-snapshot
Tailoring Escherichia coli for the l -Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins
inferred← citeshttps://doi.org/10.1021/acssynbio.7b00102openalex-snapshot
Tunable Control of an Escherichia coli Expression System for the Overproduction of Membrane Proteins by Titrated Expression of a Mutant lac Repressor
inferred← citeshttps://doi.org/10.1021/acssynbio.9b00120openalex-snapshot
Optimization of Recombinant Membrane Protein Production in the Engineered Escherichia coli Strains SuptoxD and SuptoxR
inferred← citeshttps://doi.org/10.1021/bi300010wopenalex-snapshot
Membrane Protein Structure Determination Using Crystallography and Lipidic Mesophases: Recent Advances and Successes
inferred← citeshttps://doi.org/10.1021/bi401644sopenalex-snapshot
Purification and Biophysical Characterization of the CapA Membrane Protein FTT0807 from Francisella tularensis
inferred← citeshttps://doi.org/10.1038/emboj.2012.157openalex-snapshot
Alternating access mechanism in the POT family of oligopeptide transporters
inferred← citeshttps://doi.org/10.1038/nature10450openalex-snapshot
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT
inferred← citeshttps://doi.org/10.1038/nature11683openalex-snapshot
Structure of the TatC core of the twin-arginine protein transport system
inferred← citeshttps://doi.org/10.1038/nature12484openalex-snapshot
A two-domain elevator mechanism for sodium/proton antiport
inferred← citeshttps://doi.org/10.1038/nature13419openalex-snapshot
Membrane proteins bind lipids selectively to modulate their structure and function
inferred← citeshttps://doi.org/10.1038/nbt.1833openalex-snapshot
Overcoming barriers to membrane protein structure determination
inferred← citeshttps://doi.org/10.1038/nprot.2008.44openalex-snapshot
GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae
inferred← citeshttps://doi.org/10.1038/nprot.2013.024openalex-snapshot
Mass spectrometry of intact membrane protein complexes
inferred← citeshttps://doi.org/10.1038/nprot.2016.088openalex-snapshot
A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies
inferred← citeshttps://doi.org/10.1038/nsmb.1788openalex-snapshot
Crystal structure of the carnitine transporter and insights into the antiport mechanism
inferred← citeshttps://doi.org/10.1038/s41467-018-03066-6openalex-snapshot
Structural basis for amino acid transport by the CAT family of SLC7 transporters
inferred← citeshttps://doi.org/10.1038/s41467-018-05969-wopenalex-snapshot
Revealing the mechanisms of membrane protein export by virulence-associated bacterial secretion systems
inferred← citeshttps://doi.org/10.1038/s41467-018-06702-3openalex-snapshot
An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
inferred← citeshttps://doi.org/10.1038/s41467-024-49082-7openalex-snapshot
Structure and mechanism of the K+/H+ exchanger KefC
inferred← citeshttps://doi.org/10.1038/s41467-025-66618-7openalex-snapshot
Structural basis of specific lysine transport by Pseudomonas aeruginosa permease LysP
inferred← citeshttps://doi.org/10.1038/s41564-020-00823-6openalex-snapshot
Structure and mechanism of the proton-driven motor that powers type 9 secretion and gliding motility
inferred← citeshttps://doi.org/10.1038/s41586-022-05675-0openalex-snapshot
Population-based heteropolymer design to mimic protein mixtures
inferred← citeshttps://doi.org/10.1038/s41596-021-00619-wopenalex-snapshot
The GFP thermal shift assay for screening ligand and lipid interactions to solute carrier transporters
inferred← citeshttps://doi.org/10.1038/s41596-021-00660-9openalex-snapshot
Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP
inferred← citeshttps://doi.org/10.1038/s41598-017-15711-zopenalex-snapshot
Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity
inferred← citeshttps://doi.org/10.1038/s41598-018-32579-9openalex-snapshot
ADIPOR1 is essential for vision and its RPE expression is lost in the Mfrprd6 mouse
inferred← citeshttps://doi.org/10.1038/s41598-019-39382-0openalex-snapshot
Functionality of membrane proteins overexpressed and purified from E. coli is highly dependent upon the strain
inferred← citeshttps://doi.org/10.1038/s41598-019-41309-8openalex-snapshot
Specific modification at the C-terminal lysine residue of the green fluorescent protein variant, GFPuv, expressed in Escherichia coli
inferred← citeshttps://doi.org/10.1038/s41598-019-49292-wopenalex-snapshot
Site-Directed Alkylation Detected by In-Gel Fluorescence (SDAF) to Determine the Topology Map and Probe the Solvent Accessibility of Membrane Proteins
inferred← citeshttps://doi.org/10.1038/s41598-023-43389-zopenalex-snapshot
Recovery of the histamine H3 receptor activity lost in yeast cells through error-prone PCR and in vivo selection
inferred← citeshttps://doi.org/10.1038/s41929-022-00820-4openalex-snapshot
Metabolic engineering of Escherichia coli with electron channelling for the production of natural products
inferred← citeshttps://doi.org/10.1038/s42003-021-01891-yopenalex-snapshot
Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
inferred← citeshttps://doi.org/10.1038/s42003-021-02493-4openalex-snapshot
Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity
inferred← citeshttps://doi.org/10.1038/srep00102openalex-snapshot
Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5
inferred← citeshttps://doi.org/10.1038/srep12097openalex-snapshot
Escherichia coli as host for membrane protein structure determination: a global analysis
inferred← citeshttps://doi.org/10.1038/srep16076openalex-snapshot
Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction
inferred← citeshttps://doi.org/10.1038/srep17037openalex-snapshot
Folding of newly translated membrane protein CCR5 is assisted by the chaperonin GroEL-GroES
inferred← citeshttps://doi.org/10.1038/srep45089openalex-snapshot
Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3)
inferred← citeshttps://doi.org/10.1039/c8ra06237dopenalex-snapshot
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis
inferred← citeshttps://doi.org/10.1042/bj20130619openalex-snapshot
Conserved structure and domain organization among bacterial Slc26 transporters
inferred← citeshttps://doi.org/10.1042/bst0350760openalex-snapshot
Neurotensin receptor type 1: Escherichia coli expression, purification, characterization and biophysical study
inferred← citeshttps://doi.org/10.1042/bst20150081openalex-snapshot
Characterizing diverse orthologues of the cystic fibrosis transmembrane conductance regulator protein for structural studies
inferred← citeshttps://doi.org/10.1042/bst20180140openalex-snapshot
Membrane protein engineering to the rescue
inferred← citeshttps://doi.org/10.1042/bst20200106openalex-snapshot
Current pivotal strategies leading a difficult target protein to a sample suitable for crystallographic analysis
inferred← citeshttps://doi.org/10.1071/ch14333openalex-snapshot
Membrane Protein Structures for Rational Antimicrobial Drug Design
inferred← citeshttps://doi.org/10.1073/pnas.0704546104openalex-snapshot
High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae
inferred← citeshttps://doi.org/10.1073/pnas.0802190105openalex-snapshot
Quality control of overexpressed membrane proteins
inferred← citeshttps://doi.org/10.1073/pnas.0804090105openalex-snapshot
Tuning Escherichia coli for membrane protein overexpression
inferred← citeshttps://doi.org/10.1073/pnas.1101815108openalex-snapshot
Lipid cubic phase as a membrane mimetic for integral membrane protein enzymes
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