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Protein degradation in a TX-TL cell-free expression system using ClpXP protease

https://doi.org/10.1101/019695
0 signed · 27 inferred · view as graph

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1016/j.bbamcr.2011.06.007openalex-snapshot
ClpXP, an ATP-powered unfolding and protein-degradation machine
inferredcites →https://doi.org/10.1016/s0092-8674(03)00612-3openalex-snapshot
Linkage between ATP Consumption and Mechanical Unfolding during the Protein Processing Reactions of an AAA+ Degradation Machine
inferredcites →https://doi.org/10.1016/s1097-2765(00)80243-9openalex-snapshot
Dynamics of Substrate Denaturation and Translocation by the ClpXP Degradation Machine
inferredcites →https://doi.org/10.1021/sb200016sopenalex-snapshot
An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells
inferredcites →https://doi.org/10.1021/sb300049popenalex-snapshot
Genome Replication, Synthesis, and Assembly of the Bacteriophage T7 in a Single Cell-Free Reaction
inferredcites →https://doi.org/10.1021/sb400131aopenalex-snapshot
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
inferredcites →https://doi.org/10.1021/sb400203popenalex-snapshot
Gene Circuit Performance Characterization and Resource Usage in a Cell-Free “Breadboard”
inferredcites →https://doi.org/10.1038/nature04031openalex-snapshot
Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines
inferredcites →https://doi.org/10.1039/b808034hopenalex-snapshot
Cell-free protein expression in a microchannel array with passive pumping
inferredcites →https://doi.org/10.1039/c1lc20462aopenalex-snapshot
Continuous protein production in nanoporous, picolitre volume containers
inferredcites →https://doi.org/10.1073/pnas.0404733101openalex-snapshot
SspB delivery of substrates for ClpXP proteolysis probed by the design of improved degradation tags
inferredcites →https://doi.org/10.1073/pnas.1311166110openalex-snapshot
Implementation of cell-free biological networks at steady state
inferredcites →https://doi.org/10.1073/pnas.2135496100openalex-snapshot
Principles of cell-free genetic circuit assembly
inferredcites →https://doi.org/10.1074/jbc.m109.017624openalex-snapshot
Engineering Synthetic Adaptors and Substrates for Controlled ClpXP Degradation
inferredcites →https://doi.org/10.1101/gad.12.9.1338openalex-snapshot
The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system
inferredcites →https://doi.org/10.1109/cdc.2013.6760079openalex-snapshot
An in silico modeling toolbox for rapid prototyping of circuits in a biomolecular “breadboard” system
inferredcites →https://doi.org/10.1110/ps.0237603openalex-snapshot
Energy‐dependent degradation: Linkage between ClpX‐catalyzed nucleotide hydrolysis and protein‐substrate processing
inferredcites →https://doi.org/10.1186/1754-1611-4-8openalex-snapshot
Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
inferredcites →https://doi.org/10.1186/1754-1611-4-9openalex-snapshot
Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system
inferredcites →https://doi.org/10.3791/50762openalex-snapshot
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
inferredcites →https://doi.org/10.3791/50762-vopenalex-snapshot
Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1016/j.synbio.2020.07.006openalex-snapshot
Methodologies for preparation of prokaryotic extracts for cell-free expression systems
inferred← citeshttps://doi.org/10.1016/j.ymeth.2015.05.020openalex-snapshot
Characterizing and Prototyping Genetic Networks with Cell-Free Transcription-Translation Reactions
inferred← citeshttps://doi.org/10.1021/acssynbio.7b00253openalex-snapshot
Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression
inferred← citeshttps://doi.org/10.1038/s41598-018-21739-6openalex-snapshot
Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system
inferred← citeshttps://doi.org/10.1093/synbio/ysab007openalex-snapshot
A MATLAB toolbox for modeling genetic circuits in cell-free systems†
inferred← citeshttps://doi.org/10.1101/048710openalex-snapshot
Development of prokaryotic cell-free systems for synthetic biology
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