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Development of prokaryotic cell-free systems for synthetic biology

https://doi.org/10.1101/048710
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Prolonging cell-free protein synthesis with a novel ATP regeneration system
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Cell‐Free Protein Synthesis
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Substrate replenishment extends protein synthesis with an in vitro translation system designed to mimic the cytoplasm
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Maintaining rapid growth in moderate‐density Escherichia coli fermentations
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Prolonged cell‐free protein synthesis using dual energy sources: Combined use of creatine phosphate and glucose for the efficient supply of ATP and retarded accumulation of phosphate
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Microscale to manufacturing scale‐up of cell‐free cytokine production—a new approach for shortening protein production development timelines
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Improving Cell‐Free Protein Synthesis through Genome Engineering of Escherichia coli Lacking Release Factor 1
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A sequential expression system for high‐throughput functional genomic analysis
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Acetyl Phosphate as an Energy Source for Bacterial Cell-Free Translation Systems
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Cell-Free Production of Biologically Active Polypeptides: Application to the Synthesis of Antibacterial Peptide Cecropin
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Cell-Free Synthesis of the H-Cluster: A Model for the In Vitro Assembly of Metalloprotein Metal Centers
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Calcium-dependent bacteriophage DNA infection
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A Restriction enzyme from Hemophilus influenzae
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[3] Cell-free protein synthesis directed by messenger RNA
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[25] In vitro RNA synthesis with SP6 RNA polymerase
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Disruption of microbial cells for intracellular products
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Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation
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Confusion between protectin D1 (PD1) and its isomer protectin DX (PDX). An overview on the dihydroxy-docosatrienes described to date
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Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system
inferredcites →https://doi.org/10.1016/j.biotechadv.2011.09.016openalex-snapshot
Cell-free protein synthesis: Applications come of age
inferredcites →https://doi.org/10.1016/j.chembiol.2010.12.019openalex-snapshot
Biohydrogenation from Biomass Sugar Mediated by In Vitro Synthetic Enzymatic Pathways
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A novel cell-free protein synthesis system
inferredcites →https://doi.org/10.1016/j.jbiotec.2005.11.011openalex-snapshot
Total amino acid stabilization during cell-free protein synthesis reactions
inferredcites →https://doi.org/10.1016/j.jbiotec.2006.05.014openalex-snapshot
Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system
inferredcites →https://doi.org/10.1016/j.jbiotec.2007.05.002openalex-snapshot
An economical and highly productive cell-free protein synthesis system utilizing fructose-1,6-bisphosphate as an energy source
inferredcites →https://doi.org/10.1016/j.nbt.2010.06.015openalex-snapshot
Rational improvement of cell-free protein synthesis
inferredcites →https://doi.org/10.1016/j.procbio.2011.03.008openalex-snapshot
Prolonged production of proteins in a cell-free protein synthesis system using polymeric carbohydrates as an energy source
inferredcites →https://doi.org/10.1016/j.ymben.2004.01.003openalex-snapshot
Amino acid stabilization for cell-free protein synthesis by modification of the Escherichia coli genome
inferredcites →https://doi.org/10.1016/j.ymben.2011.09.002openalex-snapshot
Cell-free synthetic biology: Thinking outside the cell
inferredcites →https://doi.org/10.1016/j.ymben.2014.10.007openalex-snapshot
A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system
inferredcites →https://doi.org/10.1016/j.ymben.2016.03.002openalex-snapshot
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
inferredcites →https://doi.org/10.1016/j.ymeth.2015.05.020openalex-snapshot
Characterizing and Prototyping Genetic Networks with Cell-Free Transcription-Translation Reactions
inferredcites →https://doi.org/10.1016/s0014-5793(98)01620-2openalex-snapshot
Cell‐free production and stable‐isotope labeling of milligram quantities of proteins
inferredcites →https://doi.org/10.1016/s0022-2836(66)80263-2openalex-snapshot
Isolation and characterization of ribonuclease I mutants of Escherichia coli
inferredcites →https://doi.org/10.1016/s0168-1656(00)00326-6openalex-snapshot
Expression-independent consumption of substrates in cell-free expression system from Escherichia coli
inferredcites →https://doi.org/10.1016/s0958-1669(98)80042-6openalex-snapshot
Recent advances in producing and selecting functional proteins by using cell-free translation
inferredcites →https://doi.org/10.1021/acssynbio.5b00090openalex-snapshot
Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor
inferredcites →https://doi.org/10.1021/acssynbio.5b00296openalex-snapshot
The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
inferredcites →https://doi.org/10.1021/bp000031yopenalex-snapshot
Prolonging Cell-Free Protein Synthesis by Selective Reagent Additions
inferredcites →https://doi.org/10.1021/bp034350bopenalex-snapshot
Expression of Active Murine Granulocyte-Macrophage Colony-Stimulating Factor in an Escherichia coli Cell-Free System
inferredcites →https://doi.org/10.1021/bp0400238openalex-snapshot
Enhanced Cell-Free Protein Synthesis Using a S30 Extract from Escherichia coli Grown Rapidly at 42 °C in an Amino Acid Enriched Medium
inferredcites →https://doi.org/10.1021/bp049789yopenalex-snapshot
Streamlining Escherichia Coli S30 Extract Preparation for Economical Cell-Free Protein Synthesis
inferredcites →https://doi.org/10.1021/bp050052yopenalex-snapshot
An Economical Method for Cell-Free Protein Synthesis using Glucose and Nucleoside Monophosphates
inferredcites →https://doi.org/10.1021/bp050087yopenalex-snapshot
Cell‐Free Production of Aggregation‐Prone Proteins in Soluble and Active Forms
inferredcites →https://doi.org/10.1021/bp060110vopenalex-snapshot
Cell‐Free Protein Synthesis System Prepared from Insect Cells by Freeze‐Thawing
inferredcites →https://doi.org/10.1021/bp960052lopenalex-snapshot
A Semicontinuous Prokaryotic Coupled Transcription/Translation System Using a Dialysis Membrane
inferredcites →https://doi.org/10.1021/pr050459yopenalex-snapshot
Rapid Expression of Functional Genomic Libraries
inferredcites →https://doi.org/10.1021/sb200016sopenalex-snapshot
An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells
inferredcites →https://doi.org/10.1021/sb400131aopenalex-snapshot
Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
inferredcites →https://doi.org/10.1021/sb400140topenalex-snapshot
Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation
inferredcites →https://doi.org/10.1021/sb400206copenalex-snapshot
Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems
inferredcites →https://doi.org/10.1023/a:1005624811710openalex-snapshot
Oxalate improves protein synthesis by enhancing ATP supply in a cell-free system derived from Escherichia coli
inferredcites →https://doi.org/10.1023/b:jsfg.0000029204.57846.7dopenalex-snapshot
Preparation of Escherichia coli cell extract for highly productive cell-free protein expression
inferredcites →https://doi.org/10.1038/90802openalex-snapshot
Cell-free translation reconstituted with purified components
inferredcites →https://doi.org/10.1038/msb.2008.57openalex-snapshot
An integrated cell‐free metabolic platform for protein production and synthetic biology
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A fast, robust and tunable synthetic gene oscillator
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Programming cells by multiplex genome engineering and accelerated evolution
inferredcites →https://doi.org/10.1038/nbt.1716openalex-snapshot
Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips
inferredcites →https://doi.org/10.1038/nbt0197-79openalex-snapshot
Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones
inferredcites →https://doi.org/10.1038/nbt1294-1357openalex-snapshot
Recombinant Proteins Can Be Isolated from E. coli Cells by Repeated Cycles of Freezing and Thawing
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Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
inferredcites →https://doi.org/10.1038/nmeth.1226openalex-snapshot
Mapping and quantifying mammalian transcriptomes by RNA-Seq
inferredcites →https://doi.org/10.1038/nrg1325openalex-snapshot
Advanced sequencing technologies: methods and goals
inferredcites →https://doi.org/10.1038/srep08663openalex-snapshot
High-throughput preparation methods of crude extract for robust cell-free protein synthesis
inferredcites →https://doi.org/10.1073/pnas.1417719112openalex-snapshot
High-yield hydrogen production from biomass by in vitro metabolic engineering: Mixed sugars coutilization and kinetic modeling
inferredcites →https://doi.org/10.1073/pnas.2135496100openalex-snapshot
Principles of cell-free genetic circuit assembly
inferredcites →https://doi.org/10.1073/pnas.47.10.1588openalex-snapshot
The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides
inferredcites →https://doi.org/10.1101/017814openalex-snapshot
Prototyping 1,4-butanediol (BDO) biosynthesis pathway in a cell-free transcription-translation (TX-TL) system
inferredcites →https://doi.org/10.1101/019695openalex-snapshot
Protein degradation in a TX-TL cell-free expression system using ClpXP protease
inferredcites →https://doi.org/10.1101/027656openalex-snapshot
Design Space Exploration of the Violacein Pathway in Escherichia coli Based Cell-Free System
inferredcites →https://doi.org/10.1109/mpul.2016.2514881openalex-snapshot
Cell Break: How Cell-Free Biology Is Finally Putting the Engineering Back in Bioengineering
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How fast‐growing bacteria robustly tune their ribosome concentration to approximate growth‐rate maximization
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A Highly Efficient Cell‐Free Protein Synthesis System from Escherichia coli
inferredcites →https://doi.org/10.1126/science.153.3740.1119openalex-snapshot
Polysomes Extracted from Escherichia coli by Freeze-Thaw-Lysozyme Lysis
inferredcites →https://doi.org/10.1126/science.3055301openalex-snapshot
A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield
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Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Sediment Samples
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Uptake and metabolism of sucrose by Streptococcus lactis
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IN VITRO SYNTHESIS OF PROTEIN IN MICROBIAL SYSTEMS
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Increasing PCR Fragment Stability and Protein Yields in a Cell-Free System with Genetically Modified Escherichia coli Extracts
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Cell-free protein synthesis energized by slowly-metabolized maltodextrin
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Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70
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Chemical lysis of cyanobacteria
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Methods for microbial DNA extraction from soil for PCR amplification
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Evidence for an additional disulfide reduction pathway in Escherichia coli
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Streamlined Extract Preparation for Escherichia Coli -Based Cell-Free Protein Synthesis by Sonication or Bead Vortex Mixing
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Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
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Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system
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Rapid cell-free forward engineering of novel genetic ring oscillators
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Author response: Rapid cell-free forward engineering of novel genetic ring oscillators

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1021/acssynbio.1c00195openalex-snapshot
Characterizing and Improving Reaction Times for E. coli-Based Cell-Free Protein Synthesis
inferred← citeshttps://doi.org/10.1038/s41576-019-0186-3openalex-snapshot
Cell-free gene expression: an expanded repertoire of applications
inferred← citeshttps://doi.org/10.3389/fbioe.2020.00213openalex-snapshot
Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology
inferred← citeshttps://doi.org/10.3390/vaccines12121344openalex-snapshot
Protein Expression Platforms and the Challenges of Viral Antigen Production
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