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Robust and sensitive detection of SARS-CoV-2 using PCR based methods

https://doi.org/10.1101/2020.07.03.186304
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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A method for the rapid construction of cRNA standard curves in quantitative real-time reverse transcription polymerase chain reaction
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Droplet Digital PCR for Absolute Quantification of Pathogens
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The emergence of SARS, MERS and novel SARS-2 coronaviruses in the 21st century
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Digital polymerase chain reaction for characterisation of DNA reference materials
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Comparison of reverse-transcriptase qPCR and droplet digital PCR for the quantification of dengue virus nucleic acid
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Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
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Embracing the Landscape of Therapeutics
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The Architecture of SARS-CoV-2 Transcriptome
inferredcites →https://doi.org/10.1016/j.virol.2017.08.005openalex-snapshot
Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses
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The important role of serology for COVID-19 control
inferredcites →https://doi.org/10.1021/ac202028gopenalex-snapshot
High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
inferredcites →https://doi.org/10.1021/ac202578xopenalex-snapshot
Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification
inferredcites →https://doi.org/10.1021/ac403843jopenalex-snapshot
High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
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International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction
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Stable Isotope Labeled DNA: A New Strategy for the Quantification of Total DNA Using Liquid Chromatography–Mass Spectrometry
inferredcites →https://doi.org/10.1021/acsinfecdis.0c00464openalex-snapshot
Comparative Analysis of Primer–Probe Sets for RT-qPCR of COVID-19 Causative Virus (SARS-CoV-2)
inferredcites →https://doi.org/10.1038/nature12711openalex-snapshot
Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor
inferredcites →https://doi.org/10.1038/nmeth.2633openalex-snapshot
Absolute quantification by droplet digital PCR versus analog real-time PCR
inferredcites →https://doi.org/10.1038/s41564-020-0695-zopenalex-snapshot
The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2
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A new coronavirus associated with human respiratory disease in China
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A pneumonia outbreak associated with a new coronavirus of probable bat origin
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Phenylalanine regulates initiation of digestive enzyme mRNA translation in pancreatic acinar cells and tissue segments in dairy calves
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Application of droplet digital PCR to detect the pathogens of infectious diseases
inferredcites →https://doi.org/10.1056/nejm199506013322208openalex-snapshot
Molecular Diagnosis
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A Novel Coronavirus from Patients with Pneumonia in China, 2019
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Mechanisms of Host Receptor Adaptation by Severe Acute Respiratory Syndrome Coronavirus
inferredcites →https://doi.org/10.1088/0026-1394/49/1a/08002openalex-snapshot
CCQM-K86/P113.1: Relative quantification of genomic DNA fragments extracted from a biological tissue
inferredcites →https://doi.org/10.1093/cid/ciaa345openalex-snapshot
Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients
inferredcites →https://doi.org/10.1093/clinchem/hvaa029openalex-snapshot
Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia
inferredcites →https://doi.org/10.1126/science.abb2507openalex-snapshot
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation
inferredcites →https://doi.org/10.1128/jcm.02620-12openalex-snapshot
Comparison of Droplet Digital PCR to Real-Time PCR for Quantitative Detection of Cytomegalovirus
inferredcites →https://doi.org/10.1128/jcm.43.11.5452-5456.2005openalex-snapshot
Development of One-Step, Real-Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E
inferredcites →https://doi.org/10.1128/jvi.80.9.4211-4219.2006openalex-snapshot
Animal Origins of the Severe Acute Respiratory Syndrome Coronavirus: Insight from ACE2-S-Protein Interactions
inferredcites →https://doi.org/10.1146/annurev-micro-020518-115759openalex-snapshot
Human Coronavirus: Host-Pathogen Interaction
inferredcites →https://doi.org/10.1371/journal.pone.0085999openalex-snapshot
Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA
inferredcites →https://doi.org/10.1371/journal.pone.0089680openalex-snapshot
A Selected Core Microbiome Drives the Early Stages of Three Popular Italian Cheese Manufactures
inferredcites →https://doi.org/10.1371/journal.pone.0103917openalex-snapshot
A Method for Quantitative Analysis of Standard and High-Throughput qPCR Expression Data Based on Input Sample Quantity
inferredcites →https://doi.org/10.1373/clinchem.2017.285478openalex-snapshot
Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine
inferredcites →https://doi.org/10.2807/1560-7917.es.2020.25.3.2000045openalex-snapshot
Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
inferredcites →https://doi.org/10.2807/1560-7917.es.2020.25.40.2001704openalex-snapshot
Detection of West Nile virus in a common whitethroat (Curruca communis) and Culex mosquitoes in the Netherlands, 2020
inferredcites →https://doi.org/10.2807/ese.17.39.20285-enopenalex-snapshot
Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
inferredcites →https://doi.org/10.3201/eid2201.151055openalex-snapshot
Variations in Spike Glycoprotein Gene of MERS-CoV, South Korea, 2015
inferredcites →https://doi.org/10.3389/fmicb.2019.00769openalex-snapshot
Local Epidemics Gone Viral: Evolution and Diffusion of the Italian HIV-1 Recombinant Form CRF60_BC
inferredcites →https://doi.org/10.3389/fmicb.2019.01226openalex-snapshot
Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses
inferredcites →https://doi.org/10.3389/fmicb.2019.01570openalex-snapshot
Accurate Quantification and Characterization of Adeno-Associated Viral Vectors
inferredcites →https://doi.org/10.3390/ijms21082826openalex-snapshot
A Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2
inferredcites →https://doi.org/10.3390/ijms21083004openalex-snapshot
RT-qPCR Testing of SARS-CoV-2: A Primer
inferredcites →https://doi.org/10.3390/pathogens9030186openalex-snapshot
Insights into the Recent 2019 Novel Coronavirus (SARS-CoV-2) in Light of Past Human Coronavirus Outbreaks
inferredcites →https://doi.org/10.3390/pathogens9110964openalex-snapshot
Clinical Symptoms of Arboviruses in Mexico
inferredcites →https://doi.org/10.36380/scil.2020.ojafr1openalex-snapshot
Review on coronavirus, a middle east respiratory syndrome (MERS-CoV)

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1007/s00705-022-05383-0openalex-snapshot
Diagnostic performance and clinical feasibility of a novel one-step RT-qPCR assay for simultaneous detection of multiple severe acute respiratory syndrome coronaviruses
inferred← citeshttps://doi.org/10.1016/j.bios.2021.113536openalex-snapshot
Point-of-need detection of pathogen-specific nucleic acid targets using magnetic particle spectroscopy
inferred← citeshttps://doi.org/10.21203/rs.3.rs-1027593/v1openalex-snapshot
Diagnostic Performance and Clinical Feasibility of a Novel One-Step RT-qPCR for Detecting Multiple Severe Acute Respiratory Syndrome Coronavirus
inferred← citeshttps://doi.org/10.3390/ijms21155585openalex-snapshot
Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection
inferred← citeshttps://doi.org/10.35790/ebm.v9i1.31899openalex-snapshot
Diagnostik Coronavirus Disease 2019 (COVID-19) dengan Pemeriksaan Laboratorium Mikrobiologi Klinik
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