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Counting with DNA in metabarcoding studies: how should we convert sequence reads to dietary data?

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/ece3.773openalex-snapshot
Diet analysis by next‐generation sequencing indicates the frequent consumption of introduced plants by the critically endangered red‐headed wood pigeon ( C olumba janthina nitens) in oceanic island habitats
inferredcites →https://doi.org/10.1002/jcp.1041430122openalex-snapshot
Distinct genomic copy number in mitochondria of different mammalian organs
inferredcites →https://doi.org/10.1007/s00216-014-8435-yopenalex-snapshot
Quantitative evaluation of bias in PCR amplification and next-generation sequencing derived from metabarcoding samples
inferredcites →https://doi.org/10.1007/s00294-015-0482-1openalex-snapshot
Organellar genome copy number variation and integrity during moderate maturation of roots and leaves of maize seedlings
inferredcites →https://doi.org/10.1007/s00442-014-2933-7openalex-snapshot
Pyrosequencing of prey DNA in faeces of carnivorous land snails to facilitate ecological restoration and relocation programmes
inferredcites →https://doi.org/10.1007/s10592-006-9197-7openalex-snapshot
Quantitative analysis of prey DNA in pinniped faeces: potential to estimate diet composition?
inferredcites →https://doi.org/10.1007/s10592-010-0096-6openalex-snapshot
Pyrosequencing faecal DNA to determine diet of little penguins: is what goes in what comes out?
inferredcites →https://doi.org/10.1016/j.biocon.2016.03.022openalex-snapshot
Dietary preferences of Hawaiian tree snails to inform culture for conservation
inferredcites →https://doi.org/10.1016/j.mib.2004.04.015openalex-snapshot
Molecular microbial ecology: land of the one-eyed king
inferredcites →https://doi.org/10.1038/nature12921openalex-snapshot
Fifty thousand years of Arctic vegetation and megafaunal diet
inferredcites →https://doi.org/10.1038/nature24460openalex-snapshot
Quantitative microbiome profiling links gut community variation to microbial load
inferredcites →https://doi.org/10.1038/nrg.2016.49openalex-snapshot
Coming of age: ten years of next-generation sequencing technologies
inferredcites →https://doi.org/10.1038/nrg.2017.44openalex-snapshot
Reference standards for next-generation sequencing
inferredcites →https://doi.org/10.1038/s41559-017-0413-2openalex-snapshot
Quantitative species-level ecology of reef fish larvae via metabarcoding
inferredcites →https://doi.org/10.1038/s41598-017-14984-8openalex-snapshot
Competing tradeoffs between increasing marine mammal predation and fisheries harvest of Chinook salmon
inferredcites →https://doi.org/10.1038/s41598-017-17333-xopenalex-snapshot
Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
inferredcites →https://doi.org/10.1038/srep16738openalex-snapshot
Climatic warming and the future of bison as grazers
inferredcites →https://doi.org/10.1038/srep27282openalex-snapshot
Using metabarcoding to reveal and quantify plant-pollinator interactions
inferredcites →https://doi.org/10.1038/srep41909openalex-snapshot
Molecular dietary analysis of two sympatric felids in the Mountains of Southwest China biodiversity hotspot and conservation implications
inferredcites →https://doi.org/10.1073/pnas.1503283112openalex-snapshot
DNA metabarcoding illuminates dietary niche partitioning by African large herbivores
inferredcites →https://doi.org/10.1093/aobpla/plx015openalex-snapshot
Reconstructing a herbivore’s diet using a novel rbcL DNA mini-barcode for plants
inferredcites →https://doi.org/10.1093/bfgp/elu035openalex-snapshot
Measuring differential gene expression with RNA-seq: challenges and strategies for data analysis
inferredcites →https://doi.org/10.1093/icesjms/fsm152openalex-snapshot
Diet studies of seabirds: a review and recommendations
inferredcites →https://doi.org/10.1093/jmammal/gyx070openalex-snapshot
Novel insights into the diet of the Pyrenean desman (Galemys pyrenaicus) using next-generation sequencing molecular analyses
inferredcites →https://doi.org/10.1093/oso/9780198767220.001.0001openalex-snapshot
Environmental DNA
inferredcites →https://doi.org/10.1101/124149openalex-snapshot
UNBIAS: An attempt to correct abundance bias in 16S sequencing, with limited success
inferredcites →https://doi.org/10.1111/1365-2664.12598openalex-snapshot
Quantifying relative fish abundance with eDNA : a promising tool for fisheries management
inferredcites →https://doi.org/10.1111/1755-0998.12103openalex-snapshot
Quantifying sequence proportions in a DNA ‐based diet study using Ion Torrent amplicon sequencing: which counts count?
inferredcites →https://doi.org/10.1111/1755-0998.12188openalex-snapshot
DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet
inferredcites →https://doi.org/10.1111/1755-0998.12265openalex-snapshot
Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias
inferredcites →https://doi.org/10.1111/1755-0998.12309openalex-snapshot
Detecting frogs as prey in the diets of introduced mammals: a comparison between morphological and DNA ‐based diet analyses
inferredcites →https://doi.org/10.1111/1755-0998.12355openalex-snapshot
Universal and blocking primer mismatches limit the use of high‐throughput DNA sequencing for the quantitative metabarcoding of arthropods
inferredcites →https://doi.org/10.1111/1755-0998.12402openalex-snapshot
Tag jumps illuminated – reducing sequence‐to‐sample misidentifications in metabarcoding studies
inferredcites →https://doi.org/10.1111/1755-0998.12490openalex-snapshot
Quantitative DNA metabarcoding: improved estimates of species proportional biomass using correction factors derived from control material
inferredcites →https://doi.org/10.1111/1755-0998.12951openalex-snapshot
An improved method for utilizing high‐throughput amplicon sequencing to determine the diets of insectivorous animals
inferredcites →https://doi.org/10.1111/2041-210x.12435openalex-snapshot
Epi RAD seq: scalable analysis of genomewide patterns of methylation using next‐generation sequencing
inferredcites →https://doi.org/10.1111/2041-210x.12677openalex-snapshot
Optimised scat collection protocols for dietary DNA metabarcoding in vertebrates
inferredcites →https://doi.org/10.1111/2041-210x.12849openalex-snapshot
Scrutinizing key steps for reliable metabarcoding of environmental samples
inferredcites →https://doi.org/10.1111/2041-210x.12869openalex-snapshot
Diet tracing in ecology: Method comparison and selection
inferredcites →https://doi.org/10.1111/eva.12225openalex-snapshot
Molecular detection of trophic interactions: emerging trends, distinct advantages, significant considerations and conservation applications
inferredcites →https://doi.org/10.1111/fog.12204openalex-snapshot
Puffins reveal contrasting relationships between forage fish and ocean climate in the North Pacific
inferredcites →https://doi.org/10.1111/j.1365-294x.2007.03613.xopenalex-snapshot
INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA‐based approaches
inferredcites →https://doi.org/10.1111/j.1365-294x.2009.04158.xopenalex-snapshot
Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces
inferredcites →https://doi.org/10.1111/j.1365-294x.2010.04898.xopenalex-snapshot
Quantifying microbial communities with 454 pyrosequencing: does read abundance count?
inferredcites →https://doi.org/10.1111/j.1365-294x.2011.05403.xopenalex-snapshot
Who is eating what: diet assessment using next generation sequencing
inferredcites →https://doi.org/10.1111/j.1755-0998.2008.02352.xopenalex-snapshot
New perspectives in diet analysis based on DNA barcoding and parallel pyrosequencing: the trn L approach
inferredcites →https://doi.org/10.1111/j.1755-0998.2010.02974.xopenalex-snapshot
Proportion of prey consumed can be determined from faecal DNA using real‐time PCR
inferredcites →https://doi.org/10.1111/j.2041-210x.2012.00198.xopenalex-snapshot
Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring
inferredcites →https://doi.org/10.1111/mec.12519openalex-snapshot
An inordinate fondness for beetles? Variation in seasonal dietary preferences of night‐roosting big brown bats ( Eptesicus fuscus )
inferredcites →https://doi.org/10.1111/mec.12523openalex-snapshot
Improving accuracy of DNA diet estimates using food tissue control materials and an evaluation of proxies for digestion bias
inferredcites →https://doi.org/10.1111/mec.12531openalex-snapshot
Who's for dinner? High‐throughput sequencing reveals bat dietary differentiation in a biodiversity hotspot where prey taxonomy is largely undescribed
inferredcites →https://doi.org/10.1111/mec.12552openalex-snapshot
The detectability half‐life in arthropod predator–prey research: what it is, why we need it, how to measure it, and how to use it
inferredcites →https://doi.org/10.1111/mec.12831openalex-snapshot
Special issue on molecular detection of trophic interactions: Unpicking the tangled bank
inferredcites →https://doi.org/10.1111/mec.13481openalex-snapshot
Assessing vertebrate biodiversity in a kelp forest ecosystem using environmental DNA
inferredcites →https://doi.org/10.1111/mec.13564openalex-snapshot
What you need is what you eat? Prey selection by the batMyotis daubentonii
inferredcites →https://doi.org/10.1111/mec.14245openalex-snapshot
High occurrence of jellyfish predation by black‐browed and Campbell albatross identified by DNA metabarcoding
inferredcites →https://doi.org/10.1111/mec.14474openalex-snapshot
Agriculture shapes the trophic niche of a bat preying on multiple pest arthropods across Europe: Evidence from DNA metabarcoding
inferredcites →https://doi.org/10.1111/nph.12923openalex-snapshot
Parsing ecological signal from noise in next generation amplicon sequencing
inferredcites →https://doi.org/10.1139/cjfas-2015-0558openalex-snapshot
Harbour seals target juvenile salmon of conservation concern
inferredcites →https://doi.org/10.1139/cjz-2016-0253openalex-snapshot
Diet of endangered Steller sea lions (Eumetopias jubatus) in the Aleutian Islands: new insights from DNA detections and bioenergetic reconstructions
inferredcites →https://doi.org/10.1186/1742-9994-6-16openalex-snapshot
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
inferredcites →https://doi.org/10.1186/s13059-018-1408-2openalex-snapshot
Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA methylation data
inferredcites →https://doi.org/10.1371/journal.pone.0058971openalex-snapshot
A DNA Metabarcoding Study of a Primate Dietary Diversity and Plasticity across Its Entire Fragmented Range
inferredcites →https://doi.org/10.1371/journal.pone.0082227openalex-snapshot
Adélie Penguin Population Diet Monitoring by Analysis of Food DNA in Scats
inferredcites →https://doi.org/10.1371/journal.pone.0083152openalex-snapshot
From Puffins to Plankton: A DNA-Based Analysis of a Seabird Food Chain in the Northern Gulf of Maine
inferredcites →https://doi.org/10.1371/journal.pone.0099722openalex-snapshot
The Bias Associated with Amplicon Sequencing Does Not Affect the Quantitative Assessment of Bacterial Community Dynamics
inferredcites →https://doi.org/10.1371/journal.pone.0124671openalex-snapshot
From Benchtop to Desktop: Important Considerations when Designing Amplicon Sequencing Workflows
inferredcites →https://doi.org/10.1371/journal.pone.0130324openalex-snapshot
Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass—Sequence Relationships with an Innovative Metabarcoding Protocol
inferredcites →https://doi.org/10.1371/journal.pone.0144956openalex-snapshot
Estimation of a Killer Whale (Orcinus orca) Population’s Diet Using Sequencing Analysis of DNA from Feces
inferredcites →https://doi.org/10.1371/journal.pone.0157977openalex-snapshot
Diet Assessment Based on Rumen Contents: A Comparison between DNA Metabarcoding and Macroscopy
inferredcites →https://doi.org/10.1371/journal.pone.0159133openalex-snapshot
DNA Metabarcoding Reveals Diet Overlap between the Endangered Walia Ibex and Domestic Goats - Implications for Conservation
inferredcites →https://doi.org/10.1371/journal.pone.0165252openalex-snapshot
Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes
inferredcites →https://doi.org/10.1515/dna-2015-0021openalex-snapshot
The applicability of DNA barcoding for dietary analysis of sika deer
inferredcites →https://doi.org/10.1642/auk-15-176.1openalex-snapshot
Diet reconstruction using next-generation sequencing increases the known ecosystem usage by a shorebird
inferredcites →https://doi.org/10.3354/meps12165openalex-snapshot
Assessing the trophic ecology of top predators across a recolonisation frontier using DNA metabarcoding of diets
inferredcites →https://doi.org/10.3389/fevo.2016.00015openalex-snapshot
Within-Population Isotopic Niche Variability in Savanna Mammals: Disparity between Carnivores and Herbivores
inferredcites →https://doi.org/10.5061/dryad.f5f60openalex-snapshot
Data from: Diet tracing in ecology: method comparison and selection
inferredcites →https://doi.org/10.7287/peerj.preprints.3184v1openalex-snapshot
An improved method for utilizing high-throughput amplicon sequencing to determine the diets of insectivorous animals

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1002/ece3.4559openalex-snapshot
Table for five, please: Dietary partitioning in boreal bats
inferred← citeshttps://doi.org/10.1002/ece3.4787openalex-snapshot
From feces to data: A metabarcoding method for analyzing consumed and available prey in a bird‐insect food web
inferred← citeshttps://doi.org/10.1002/ece3.4858openalex-snapshot
DNA metabarcoding assays reveal a diverse prey assemblage for Mobula rays in the Bohol Sea, Philippines
inferred← citeshttps://doi.org/10.1002/ece3.4866openalex-snapshot
Messy eaters: Swabbing prey DNA from the exterior of inconspicuous predators when foraging cannot be observed
inferred← citeshttps://doi.org/10.1002/ece3.5901openalex-snapshot
Insectivorous bats selectively source moths and eat mostly pest insects on dryland and irrigated cotton farms
inferred← citeshttps://doi.org/10.1002/ps.70023openalex-snapshot
Common insectivorous bats may serve as a natural solution for suppressing pests, with implications for managing apple orchards in China
inferred← citeshttps://doi.org/10.1016/j.envint.2019.05.011openalex-snapshot
Can bacterial indicators of a grassy woodland restoration inform ecosystem assessment and microbiota-mediated human health?
inferred← citeshttps://doi.org/10.1038/s41598-018-34430-7openalex-snapshot
Evaluating metabarcoding to analyse diet composition of species foraging in anthropogenic landscapes using Ion Torrent and Illumina sequencing
inferred← citeshttps://doi.org/10.1038/s41598-021-96856-wopenalex-snapshot
Secondary predation constrains DNA-based diet reconstruction in two threatened shark species
inferred← citeshttps://doi.org/10.1038/s42003-025-07948-6openalex-snapshot
How individual variation shapes ecological niches in two Pipistrellus bat species
inferred← citeshttps://doi.org/10.1093/icesjms/fsz021openalex-snapshot
Time-series metabarcoding analysis of zooplankton diversity of the NW Atlantic continental shelf
inferred← citeshttps://doi.org/10.1101/2019.12.13.875898openalex-snapshot
Comparison of mechanical sorting and DNA metabarcoding for diet analysis with degraded wolf scats
inferred← citeshttps://doi.org/10.1101/2020.07.03.186346openalex-snapshot
Using DNA metabarcoding to investigate diet and niche partitioning in the native European otter ( Lutra lutra ) and invasive American mink ( Neovison vison )
inferred← citeshttps://doi.org/10.1111/1365-2745.13113openalex-snapshot
Trophic ecology of large herbivores in a reassembling African ecosystem
inferred← citeshttps://doi.org/10.1111/1440-1703.1062openalex-snapshot
Genetic and ecological conservation issues for oceanic island birds, revealed by a combination of the latest molecular techniques and conventional field work
inferred← citeshttps://doi.org/10.1111/1744-7917.12992openalex-snapshot
Development of a multiprimer metabarcoding approach to understanding trophic interactions in agroecosystems
inferred← citeshttps://doi.org/10.1111/1749-4877.12947openalex-snapshot
Honey, What's for Dinner? Dietary Overlap and Size Dimorphism Between Female and Male Joro Spiders ( Trichonephila clavata )
inferred← citeshttps://doi.org/10.1111/1755-0998.12974openalex-snapshot
When to use next generation sequencing or diagnostic PCR in diet analyses
inferred← citeshttps://doi.org/10.1111/eea.13396openalex-snapshot
A comparison of live versus kill pitfall traps to assess the diet of carabids through a metabarcoding approach
inferred← citeshttps://doi.org/10.1111/jen.13166openalex-snapshot
Aphidophagous predators in commercial Capsicum cultivars and characterization of their trophic network using stomach content analysis
inferred← citeshttps://doi.org/10.1111/jzo.12778openalex-snapshot
Ant‐eating spider maintains specialist diet throughout its ontogeny
inferred← citeshttps://doi.org/10.1111/mec.14810openalex-snapshot
Finding flies in the mushroom soup: Host specificity of fungus‐associated communities revisited with a novel molecular method
inferred← citeshttps://doi.org/10.1111/mec.15724openalex-snapshot
Leech blood‐meal invertebrate‐derived DNA reveals differences in Bornean mammal diversity across habitats
inferred← citeshttps://doi.org/10.1111/mec.17712openalex-snapshot
Global Availability of Plant DNA Barcodes as Genomic Resources to Support Basic and Policy‐Relevant Biodiversity Research
inferred← citeshttps://doi.org/10.12933/therya-22-2099openalex-snapshot
Daytime diet of the lesser sac-winged bat (Saccopteryx leptura) in a Colombian Pacific Island
inferred← citeshttps://doi.org/10.12933/therya-24-5746openalex-snapshot
Applicability of DNA barcoding-based analyses on the diet of the gray brocket deer (Subulo gouazoubira) in xeric hillside forests
inferred← citeshttps://doi.org/10.1371/journal.pone.0219135openalex-snapshot
The menu varies with metabarcoding practices: A case study with the bat Plecotus auritus
inferred← citeshttps://doi.org/10.1371/journal.pone.0220081openalex-snapshot
Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination
inferred← citeshttps://doi.org/10.1371/journal.pone.0236077openalex-snapshot
Metabarcoding of native and invasive species in stomach contents of Great Lakes fishes
inferred← citeshttps://doi.org/10.17520/biods.2019101openalex-snapshot
Metabarcoding diet analysis of snow leopards (Panthera uncia) in Wolong National Nature Reserve, Sichuan Province
inferred← citeshttps://doi.org/10.21203/rs.3.rs-465807/v1openalex-snapshot
Hammer Chyme: The Russian-Doll Effect Limits Reconstructing Marine Trophic Food Webs
inferred← citeshttps://doi.org/10.21203/rs.3.rs-941888/v1openalex-snapshot
Back Into the Wild: Feces DNA Analyses Track the Return of a Beluga Whale Back to Its Natural Environment
inferred← citeshttps://doi.org/10.22541/au.158880251.12111301openalex-snapshot
The structure of plant--herbivore ecological networks varies along elevation gradients
inferred← citeshttps://doi.org/10.22541/au.162936514.40253306/v1openalex-snapshot
For all audiences: Incorporating immature stages into standardised inventories of mega-diverse groups has a major impact on our understanding of biodiversity patterns
inferred← citeshttps://doi.org/10.26686/wgtn.14670114openalex-snapshot
Ecological impact and spread of an invasive paper wasp in New Zealand (Hymenoptera: Vespidae)
inferred← citeshttps://doi.org/10.26686/wgtn.14670114.v1openalex-snapshot
Ecological impact and spread of an invasive paper wasp in New Zealand (Hymenoptera: Vespidae)
inferred← citeshttps://doi.org/10.32942/x23g71openalex-snapshot
The rarity of Invertebrates prevents reliable application of IUCN Red-List criteria.
inferred← citeshttps://doi.org/10.3389/fcosc.2024.1377673openalex-snapshot
What bycatch tells us about the diet of harbor and gray seals and overlap with commercial fishermen
inferred← citeshttps://doi.org/10.3390/books978-3-0365-3696-5openalex-snapshot
The Ecological Role of Salamanders as Predators and Prey
inferred← citeshttps://doi.org/10.3410/f.739752285.793583465openalex-snapshot
Faculty Opinions recommendation of Passive eDNA collection enhances aquatic biodiversity analysis.
inferred← citeshttps://doi.org/10.5204/thesis.eprints.203859openalex-snapshot
Phylogeography, habitat and resource use of Nyctimene robinsoni
inferred← citeshttps://doi.org/10.54612/a.6429m7bfe1openalex-snapshot
Causes and consequences of the round goby invasion in the Baltic Sea and beyond
inferred← citeshttps://doi.org/10.70675/dcb55528z01c5z458azbecfz66e8910e8a1copenalex-snapshot
Contribution des insectes aquatiques émergeant des rivières à la fourniture de services écosystémiques pour l’agriculture
inferred← citeshttps://doi.org/10.7717/peerj.11897openalex-snapshot
Dung beetles as samplers of mammals in Malaysian Borneo—a test of high throughput metabarcoding of iDNA
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