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UNBIAS: An attempt to correct abundance bias in 16S sequencing, with limited success

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Ecological Diversity and Its Measurement
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Enhancement of PCR Amplification of Moderate GC-Containing and Highly GC-Rich DNA Sequences
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Bias and Artifacts in Multitemplate Polymerase Chain Reactions(PCR)
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A versatile curve-fit model for linear to deeply concave rank abundance curves
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Numerical Taxonomy
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Resistance and resilience of the forest soil microbiome to logging-associated compaction
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Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
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The human microbiome: at the interface of health and disease
inferredcites →https://doi.org/10.1038/nrmicro3330openalex-snapshot
Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences
inferredcites →https://doi.org/10.1093/bioinformatics/btq461openalex-snapshot
Search and clustering orders of magnitude faster than BLAST
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Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform
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Regionalized GC content of template DNA as a predictor of PCR success
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VII. Note on regression and inheritance in the case of two parents
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Phylogenetic diversity measures based on Hill numbers
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SINAPS: Prediction of microbial traits from marker gene sequences
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THE DISTRIBUTION OF THE FLORA IN THE ALPINE ZONE. 1
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Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis
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The global ocean microbiome
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Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
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Bias in Template-to-Product Ratios in Multitemplate PCR
inferredcites →https://doi.org/10.1146/annurev-pathol-011811-132421openalex-snapshot
Human Microbiome in Health and Disease
inferredcites →https://doi.org/10.1371/journal.pcbi.1002743openalex-snapshot
Incorporating 16S Gene Copy Number Information Improves Estimates of Microbial Diversity and Abundance
inferredcites →https://doi.org/10.1371/journal.pone.0043093openalex-snapshot
PCR Biases Distort Bacterial and Archaeal Community Structure in Pyrosequencing Datasets
inferredcites →https://doi.org/10.1371/journal.pone.0057923openalex-snapshot
The Variability of the 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses
inferredcites →https://doi.org/10.1371/journal.pone.0094249openalex-snapshot
Analysis, Optimization and Verification of Illumina-Generated 16S rRNA Gene Amplicon Surveys
inferredcites →https://doi.org/10.1371/journal.pone.0165015openalex-snapshot
Filtration and Normalization of Sequencing Read Data in Whole-Metagenome Shotgun Samples
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Length and GC-Biases During Sequencing Library Amplification: A Comparison of Various Polymerase-Buffer Systems with Ancient and Modern DNA Sequencing Libraries
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An Ordination of the Upland Forest Communities of Southern Wisconsin
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Accuracy of microbial community diversity estimated by closed- and open-reference OTUs

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Dadasnake, a Snakemake implementation of DADA2 to process amplicon sequencing data for microbial ecology
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Alpha diversity metrics for noisy OTUs
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Peer Review #2 of "Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data (v0.1)"
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Consistent and correctable bias in metagenomic sequencing experiments
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Accuracy of microbial community diversity estimated by closed- and open-reference OTUs
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Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data
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