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Allele-specific genome editing using CRISPR-Cas9 causes off-target mutations in diploid yeast

https://doi.org/10.1101/397984
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1016/j.cell.2013.08.021openalex-snapshot
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
inferredcites →https://doi.org/10.1016/j.celrep.2018.01.045openalex-snapshot
Hybridization Kinetics Explains CRISPR-Cas Off-Targeting Rules
inferredcites →https://doi.org/10.1016/j.dnarep.2016.05.001openalex-snapshot
The democratization of gene editing: Insights from site-specific cleavage and double-strand break repair
inferredcites →https://doi.org/10.1016/s0076-6879(02)50957-5openalex-snapshot
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method
inferredcites →https://doi.org/10.1016/s0168-9525(00)02022-9openalex-snapshot
Partners and pathways
inferredcites →https://doi.org/10.1016/s1097-2765(00)80202-6openalex-snapshot
Brca1 Controls Homology-Directed DNA Repair
inferredcites →https://doi.org/10.1021/sb500366vopenalex-snapshot
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
inferredcites →https://doi.org/10.1038/329451a0openalex-snapshot
Loss of heterozygosity of chromosome 3p markers in small-cell lung cancer
inferredcites →https://doi.org/10.1038/cr.2008.1openalex-snapshot
Homologous recombination in DNA repair and DNA damage tolerance
inferredcites →https://doi.org/10.1038/nature17946openalex-snapshot
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
inferredcites →https://doi.org/10.1038/nature23305openalex-snapshot
Correction of a pathogenic gene mutation in human embryos
inferredcites →https://doi.org/10.1038/nbt.1754openalex-snapshot
Integrative genomics viewer
inferredcites →https://doi.org/10.1038/nbt.2842openalex-snapshot
CRISPR-Cas systems for editing, regulating and targeting genomes
inferredcites →https://doi.org/10.1038/nbt.3198openalex-snapshot
Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells
inferredcites →https://doi.org/10.1038/nbt.3816openalex-snapshot
Highly efficient RNA-guided base editing in mouse embryos
inferredcites →https://doi.org/10.1038/nbt.4192openalex-snapshot
Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
inferredcites →https://doi.org/10.1038/nmeth.2857openalex-snapshot
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
inferredcites →https://doi.org/10.1038/nrg.2015.34openalex-snapshot
Cheating evolution: engineering gene drives to manipulate the fate of wild populations
inferredcites →https://doi.org/10.1038/s41598-017-04686-6openalex-snapshot
CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus
inferredcites →https://doi.org/10.1073/pnas.1606701113openalex-snapshot
Pathway swapping: Toward modular engineering of essential cellular processes
inferredcites →https://doi.org/10.1073/pnas.88.15.6486openalex-snapshot
Loss of heterozygosity and mitotic linkage maps in the mouse.
inferredcites →https://doi.org/10.1093/bioinformatics/btp352openalex-snapshot
The Sequence Alignment/Map format and SAMtools
inferredcites →https://doi.org/10.1093/bioinformatics/btp698openalex-snapshot
Fast and accurate long-read alignment with Burrows–Wheeler transform
inferredcites →https://doi.org/10.1093/femsyr/fov004openalex-snapshot
CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae
inferredcites →https://doi.org/10.1093/femsyr/fov005openalex-snapshot
S. cerevisiae × S. eubayanus interspecific hybrid, the best of both worlds and beyond
inferredcites →https://doi.org/10.1093/femsyr/fox074openalex-snapshot
Nanopore sequencing enables near-complete de novo assembly of Saccharomyces cerevisiae reference strain CEN.PK113-7D
inferredcites →https://doi.org/10.1093/nar/gkt135openalex-snapshot
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
inferredcites →https://doi.org/10.1099/00221287-136-3-395openalex-snapshot
Physiology of Saccharomyces Cerevisiae in Anaerobic Glucose-Limited Chemostat Culturesx
inferredcites →https://doi.org/10.1101/cshperspect.a012740openalex-snapshot
Repair of Strand Breaks by Homologous Recombination
inferredcites →https://doi.org/10.1111/1567-1364.12024openalex-snapshot
amdSYM, a new dominant recyclable marker cassette forSaccharomyces cerevisiae
inferredcites →https://doi.org/10.1126/science.1232033openalex-snapshot
RNA-Guided Human Genome Engineering via Cas9
inferredcites →https://doi.org/10.1126/science.274.5287.546openalex-snapshot
Life with 6000 Genes
inferredcites →https://doi.org/10.1126/science.aaf5124openalex-snapshot
CRISPR-directed mitotic recombination enables genetic mapping without crosses
inferredcites →https://doi.org/10.1128/aem.03206-16openalex-snapshot
Industrial Relevance of Chromosomal Copy Number Variation in Saccharomyces Yeasts
inferredcites →https://doi.org/10.1128/aem.68.5.2095-2100.2002openalex-snapshot
Auxotrophic Yeast Strains in Fundamental and Applied Research
inferredcites →https://doi.org/10.1128/mbio.01109-16openalex-snapshot
Analysis of Repair Mechanisms following an Induced Double-Strand Break Uncovers Recessive Deleterious Alleles in the Candida albicans Diploid Genome
inferredcites →https://doi.org/10.1186/s12934-017-0835-1openalex-snapshot
CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus
inferredcites →https://doi.org/10.1371/journal.pone.0112963openalex-snapshot
Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement
inferredcites →https://doi.org/10.3389/fmicb.2018.01786openalex-snapshot
Structural, Physiological and Regulatory Analysis of Maltose Transporter Genes in Saccharomyces eubayanus CBS 12357T

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1101/476929openalex-snapshot
Laboratory evolution of a Saccharomyces cerevisiae x S. eubayanus hybrid under simulated lager-brewing conditions: genetic diversity and phenotypic convergence
inferred← citeshttps://doi.org/10.1111/risa.13501openalex-snapshot
Safe‐by‐Design: Stakeholders’ Perceptions and Expectations of How to Deal with Uncertain Risks of Emerging Biotechnologies in the Netherlands
inferred← citeshttps://doi.org/10.1371/journal.pone.0209124openalex-snapshot
Reducing phenolic off-flavors through CRISPR-based gene editing of the FDC1 gene in Saccharomyces cerevisiae x Saccharomyces eubayanus hybrid lager beer yeasts
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