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Improved yield of AAV2 and rAAV2-retro serotypes following sugar supplementation during the viral production phase

https://doi.org/10.1101/488585
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/(sici)1097-0290(20000505)68:3<260::aid-bit4>3.0.co;2-mopenalex-snapshot
Hyperosmotic pressure enhances immunoglobulin transcription rates and secretion rates of KR12H-2 transfectoma
inferredcites →https://doi.org/10.1002/bit.20847openalex-snapshot
Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability
inferredcites →https://doi.org/10.1002/bit.260371015openalex-snapshot
Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production
inferredcites →https://doi.org/10.1002/bit.260420508openalex-snapshot
Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate
inferredcites →https://doi.org/10.1002/bit.260460605openalex-snapshot
Intracellular responses of productive hybridomas subjected to high osmotic pressure
inferredcites →https://doi.org/10.1007/s00018-004-3445-0openalex-snapshot
Novel aspects of glypican glycobiology
inferredcites →https://doi.org/10.1007/s00424-006-0195-xopenalex-snapshot
Hyperosmotic stress response: comparison with other cellular stresses
inferredcites →https://doi.org/10.1016/j.jbiotec.2011.11.016openalex-snapshot
Hyperosmotic pressure on HEK 293 cells during the growth phase, but not the production phase, improves adenovirus production
inferredcites →https://doi.org/10.1016/j.lfs.2010.10.020openalex-snapshot
Protein and DNA destabilization by osmolytes: The other side of the coin
inferredcites →https://doi.org/10.1016/j.neuron.2016.09.021openalex-snapshot
A Designer AAV Variant Permits Efficient Retrograde Access to Projection Neurons
inferredcites →https://doi.org/10.1016/s0021-9258(19)50108-9openalex-snapshot
Cell surface heparan sulfate proteoglycans.
inferredcites →https://doi.org/10.1021/bp0342203openalex-snapshot
Hyperosmotic Stress in Murine Hybridoma Cells: Effects on Antibody Transcription, Translation, Posttranslational Processing, and the Cell Cycle
inferredcites →https://doi.org/10.1038/nature16465openalex-snapshot
An essential receptor for adeno-associated virus infection
inferredcites →https://doi.org/10.1042/0264-6021:3440827openalex-snapshot
Decorin endocytosis: structural features of heparin and heparan sulphate oligosaccharides interfering with receptor binding and endocytosis
inferredcites →https://doi.org/10.1042/0264-6021:3510607openalex-snapshot
Transmembrane and cytoplasmic domains of syndecan mediate a multi-step endocytic pathway involving detergent-insoluble membrane rafts
inferredcites →https://doi.org/10.1074/jbc.m112.369389openalex-snapshot
Clathrin-mediated Endocytosis and Subsequent Endo-Lysosomal Trafficking of Adeno-associated Virus/Phage
inferredcites →https://doi.org/10.1083/jcb.108.2.389openalex-snapshot
Hypertonic media inhibit receptor-mediated endocytosis by blocking clathrin-coated pit formation.
inferredcites →https://doi.org/10.1083/jcb.121.1.61openalex-snapshot
Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification.
inferredcites →https://doi.org/10.1089/hgtb.2011.034openalex-snapshot
Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences
inferredcites →https://doi.org/10.1089/hum.2010.107openalex-snapshot
Efficient Serotype-Dependent Release of Functional Vector into the Culture Medium During Adeno-Associated Virus Manufacturing
inferredcites →https://doi.org/10.1101/434589openalex-snapshot
High-performance GFP-based calcium indicators for imaging activity in neuronal populations and microcompartments
inferredcites →https://doi.org/10.1113/jphysiol.2003.058412openalex-snapshot
Roles of compatible osmolytes and heat shock protein 70 in the induction of tolerance to stresses in porcine endothelial cells
inferredcites →https://doi.org/10.1128/jvi.72.2.1438-1445.1998openalex-snapshot
Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions
inferredcites →https://doi.org/10.1152/physrev.00056.2006openalex-snapshot
Cellular Response to Hyperosmotic Stresses

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1002/bit.28412openalex-snapshot
Critical challenges and advances in recombinant adeno‐associated virus (rAAV) biomanufacturing
inferred← citeshttps://doi.org/10.1002/bit.28737openalex-snapshot
Removal of empty capsids from high‐dose adeno‐associated virus 9 gene therapies
inferred← citeshttps://doi.org/10.1002/cam4.2935openalex-snapshot
MicroRNA‐based recombinant AAV vector assembly improves efficiency of suicide gene transfer in a murine model of lymphoma
inferred← citeshttps://doi.org/10.1016/j.biotechadv.2024.108433openalex-snapshot
The state of technological advancement to address challenges in the manufacture of rAAV gene therapies
inferred← citeshttps://doi.org/10.1016/j.ejpb.2023.07.002openalex-snapshot
The ice age – A review on formulation of Adeno-associated virus therapeutics
inferred← citeshttps://doi.org/10.1016/j.xphs.2021.03.024openalex-snapshot
Manufacturing Challenges and Rational Formulation Development for AAV Viral Vectors
inferred← citeshttps://doi.org/10.1038/s41467-022-31673-xopenalex-snapshot
A luciferase prosubstrate and a red bioluminescent calcium indicator for imaging neuronal activity in mice
inferred← citeshttps://doi.org/10.1038/s41592-021-01078-7openalex-snapshot
RETRACTED ARTICLE: A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin
inferred← citeshttps://doi.org/10.1038/s41592-021-01148-wopenalex-snapshot
Retraction Note: A fast, high-affinity fluorescent serotonin biosensor engineered from a tick lipocalin
inferred← citeshttps://doi.org/10.1096/fj.202200840rropenalex-snapshot
Development of a high‐yield, high‐quality purification process for adeno‐associated virus vectors that can be used in vivo without ultracentrifugation: Application to a lung endothelial cell‐targeted adeno‐associated virus
inferred← citeshttps://doi.org/10.1126/sciadv.add2058openalex-snapshot
A genetically encoded far-red fluorescent indicator for imaging synaptically released Zn 2+
inferred← citeshttps://doi.org/10.1186/s41120-025-00131-5openalex-snapshot
Optimizing laboratory practices for recombinant adeno-associated viral vectors: impact of stress factors on vector stability
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