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Cas12a trans-cleavage can be modulated in vitro and is active on ssDNA, dsDNA, and RNA

https://doi.org/10.1101/600890
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1016/0092-8674(94)90280-1openalex-snapshot
Atomic structure of the RuvC resolvase: A holliday junction-specific endonuclease from E. coli
inferredcites →https://doi.org/10.1016/j.cell.2014.02.001openalex-snapshot
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
inferredcites →https://doi.org/10.1016/j.cell.2015.09.038openalex-snapshot
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
inferredcites →https://doi.org/10.1016/j.cell.2015.12.035openalex-snapshot
Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering
inferredcites →https://doi.org/10.1016/j.cell.2016.04.003openalex-snapshot
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA
inferredcites →https://doi.org/10.1016/j.cell.2018.10.045openalex-snapshot
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity
inferredcites →https://doi.org/10.1016/j.mib.2017.05.008openalex-snapshot
Diversity, classification and evolution of CRISPR-Cas systems
inferredcites →https://doi.org/10.1016/j.molcel.2017.03.016openalex-snapshot
Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a
inferredcites →https://doi.org/10.1016/j.molcel.2017.04.019openalex-snapshot
Structural Basis for the Altered PAM Recognition by Engineered CRISPR-Cpf1
inferredcites →https://doi.org/10.1016/j.molcel.2017.09.007openalex-snapshot
The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit
inferredcites →https://doi.org/10.1016/j.molcel.2018.06.043openalex-snapshot
Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a
inferredcites →https://doi.org/10.1016/j.molcel.2018.11.021openalex-snapshot
Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a.
inferredcites →https://doi.org/10.1016/j.tibs.2009.05.002openalex-snapshot
CRISPR-based adaptive and heritable immunity in prokaryotes
inferredcites →https://doi.org/10.1021/bi702363uopenalex-snapshot
Predicting Stability of DNA Duplexes in Solutions Containing Magnesium and Monovalent Cations
inferredcites →https://doi.org/10.1021/jacs.7b13050openalex-snapshot
Bidirectional Degradation of DNA Cleavage Products Catalyzed by CRISPR/Cas9
inferredcites →https://doi.org/10.1038/cr.2016.88openalex-snapshot
Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target DNA recognition
inferredcites →https://doi.org/10.1038/nature09886openalex-snapshot
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
inferredcites →https://doi.org/10.1038/nature13011openalex-snapshot
DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
inferredcites →https://doi.org/10.1038/nature17945openalex-snapshot
The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA
inferredcites →https://doi.org/10.1038/nature22398openalex-snapshot
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
inferredcites →https://doi.org/10.1038/nbt.2508openalex-snapshot
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
inferredcites →https://doi.org/10.1038/nbt.3609openalex-snapshot
Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells
inferredcites →https://doi.org/10.1038/nbt.3620openalex-snapshot
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
inferredcites →https://doi.org/10.1038/nbt.3900openalex-snapshot
Engineered Cpf1 variants with altered PAM specificities
inferredcites →https://doi.org/10.1038/ncomms15179openalex-snapshot
CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum
inferredcites →https://doi.org/10.1038/nprot.2013.143openalex-snapshot
Genome engineering using the CRISPR-Cas9 system
inferredcites →https://doi.org/10.1038/nrg2749openalex-snapshot
CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea
inferredcites →https://doi.org/10.1038/s41422-018-0022-xopenalex-snapshot
CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA
inferredcites →https://doi.org/10.1038/s41467-017-01836-2openalex-snapshot
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
inferredcites →https://doi.org/10.1038/s41467-018-05245-xopenalex-snapshot
Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
inferredcites →https://doi.org/10.1038/s41467-018-05641-3openalex-snapshot
Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
inferredcites →https://doi.org/10.1038/s41467-018-06129-wopenalex-snapshot
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
inferredcites →https://doi.org/10.1038/s41587-018-0011-0openalex-snapshot
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
inferredcites →https://doi.org/10.1073/pnas.1208507109openalex-snapshot
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
inferredcites →https://doi.org/10.1073/pnas.1718686115openalex-snapshot
Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
inferredcites →https://doi.org/10.1080/15476286.2018.1551703openalex-snapshot
Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA
inferredcites →https://doi.org/10.1091/mbc.e10-08-0683openalex-snapshot
A Guide to Simple and Informative Binding Assays
inferredcites →https://doi.org/10.1093/nar/gki439openalex-snapshot
enoLOGOS: a versatile web tool for energy normalized sequence logos
inferredcites →https://doi.org/10.1093/nar/gks1015openalex-snapshot
Structural asymmetry in the Thermus thermophilus RuvC dimer suggests a basis for sequential strand cleavages during Holliday junction resolution
inferredcites →https://doi.org/10.1093/nar/gkv899openalex-snapshot
Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes
inferredcites →https://doi.org/10.1093/nar/gkz184openalex-snapshot
Enhanced Cas12a editing in mammalian cells and zebrafish
inferredcites →https://doi.org/10.1126/science.1179555openalex-snapshot
CRISPR/Cas, the Immune System of Bacteria and Archaea
inferredcites →https://doi.org/10.1126/science.1225829openalex-snapshot
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
inferredcites →https://doi.org/10.1126/science.1231143openalex-snapshot
Multiplex Genome Engineering Using CRISPR/Cas Systems
inferredcites →https://doi.org/10.1126/science.1232033openalex-snapshot
RNA-Guided Human Genome Engineering via Cas9
inferredcites →https://doi.org/10.1126/science.aaq0179openalex-snapshot
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
inferredcites →https://doi.org/10.1126/science.aar6245openalex-snapshot
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
inferredcites →https://doi.org/10.1126/science.aat5011openalex-snapshot
CRISPR-Cas guides the future of genetic engineering
inferredcites →https://doi.org/10.1126/science.aau5138openalex-snapshot
Systematic discovery of natural CRISPR-Cas12a inhibitors
inferredcites →https://doi.org/10.1126/science.aav7271openalex-snapshot
Functionally diverse type V CRISPR-Cas systems
inferredcites →https://doi.org/10.1128/aem.00947-17openalex-snapshot
CRISPR-Cas12a-Assisted Recombineering in Bacteria
inferredcites →https://doi.org/10.1186/s13059-019-1620-8openalex-snapshot
Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
inferredcites →https://doi.org/10.7554/elife.00471openalex-snapshot
RNA-programmed genome editing in human cells

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.1007/s00018-019-03396-8openalex-snapshot
Cas12a mediates efficient and precise endogenous gene tagging via MITI: microhomology-dependent targeted integrations
inferred← citeshttps://doi.org/10.1007/s12161-023-02500-wopenalex-snapshot
Variety Differentiation: Development of a CRISPR DETECTR Method for the Detection of Single Nucleotide Polymorphisms (SNPs) in Cacao (Theobroma cacao) and Almonds (Prunus dulcis)
inferred← citeshttps://doi.org/10.1007/s13206-022-00081-0openalex-snapshot
Highly Efficient DNA Reporter for CRISPR/Cas12a-Based Specific and Sensitive Biosensor
inferred← citeshttps://doi.org/10.1016/bs.mie.2022.08.048openalex-snapshot
Optimized protocols for the characterization of Cas12a activities
inferred← citeshttps://doi.org/10.1016/j.bios.2021.113218openalex-snapshot
Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay
inferred← citeshttps://doi.org/10.1016/j.bios.2021.113382openalex-snapshot
Detection of rare variant alleles using the AsCas12a double-stranded DNA trans-cleavage activity
inferred← citeshttps://doi.org/10.1016/j.bios.2021.113624openalex-snapshot
A CRISPR/Cas12a-assisted in vitro diagnostic tool for identification and quantification of single CpG methylation sites
inferred← citeshttps://doi.org/10.1016/j.biotechadv.2025.108629openalex-snapshot
Split technology in sensors based on CRISPR/Cas12a system
inferred← citeshttps://doi.org/10.1016/j.cell.2021.01.017openalex-snapshot
Synthetic biology in the clinic: engineering vaccines, diagnostics, and therapeutics
inferred← citeshttps://doi.org/10.1016/j.isci.2021.102996openalex-snapshot
Kinetic analysis of Cas12a and Cas13a RNA-Guided nucleases for development of improved CRISPR-Based diagnostics
inferred← citeshttps://doi.org/10.1016/j.microc.2022.107606openalex-snapshot
Modulation of CRISPR/Cas12a trans-cleavage activity by various DNA-modifying enzymes
inferred← citeshttps://doi.org/10.1016/j.microc.2024.111850openalex-snapshot
Towards understanding Trans-cleavage of natural and synthetic nucleic acids by Cas12a for sensitive CRISPR biosensing
inferred← citeshttps://doi.org/10.1016/j.microc.2025.113025openalex-snapshot
Hairpin-locker mediated CRISPR/Cas tandem system for ultrasensitive detection of DNA without pre-amplification
inferred← citeshttps://doi.org/10.1016/j.omtn.2025.102568openalex-snapshot
Single-stranded HDR templates with truncated Cas12a-binding sequences improve knock-in efficiencies in primary human T cells
inferred← citeshttps://doi.org/10.1016/j.sbi.2022.102466openalex-snapshot
Miniature CRISPR-Cas12 endonucleases – Programmed DNA targeting in a smaller package
inferred← citeshttps://doi.org/10.1016/j.snb.2022.132767openalex-snapshot
Increasing trans-cleavage catalytic efficiency of Cas12a and Cas13a with chemical enhancers: Application to amplified nucleic acid detection
inferred← citeshttps://doi.org/10.1016/j.tig.2021.02.008openalex-snapshot
DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing
inferred← citeshttps://doi.org/10.1021/acs.analchem.1c04065openalex-snapshot
Universally Stable and Precise CRISPR-LAMP Detection Platform for Precise Multiple Respiratory Tract Virus Diagnosis Including Mutant SARS-CoV-2 Spike N501Y
inferred← citeshttps://doi.org/10.1021/acsnano.2c07749openalex-snapshot
Quantum Dot-Based Molecular Beacons for Quantitative Detection of Nucleic Acids with CRISPR/Cas(N) Nucleases
inferred← citeshttps://doi.org/10.1021/acssensors.0c00497openalex-snapshot
Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)
inferred← citeshttps://doi.org/10.1021/acssensors.5c05012openalex-snapshot
Multicolor Bioluminescent Toolbox for Multiplex Glow-in-the-Dark Nucleic Acid Diagnostics
inferred← citeshttps://doi.org/10.1021/acssynbio.1c00204openalex-snapshot
Recognition of DNA Target Formulations by CRISPR-Cas12a Using a dsDNA Reporter
inferred← citeshttps://doi.org/10.1021/acssynbio.1c00401openalex-snapshot
Advances in Field Detection Based on CRISPR/Cas System
inferred← citeshttps://doi.org/10.1038/s41467-020-18615-1openalex-snapshot
Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
inferred← citeshttps://doi.org/10.1038/s41467-021-21275-4openalex-snapshot
Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination
inferred← citeshttps://doi.org/10.1038/s41467-024-54491-9openalex-snapshot
Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage
inferred← citeshttps://doi.org/10.1038/s41467-025-62082-5openalex-snapshot
Identification of a key nucleotide influencing Cas12a crRNA activity for universal photo-controlled CRISPR diagnostics
inferred← citeshttps://doi.org/10.1042/bst20190564openalex-snapshot
Making the cut(s): how Cas12a cleaves target and non-target DNA
inferred← citeshttps://doi.org/10.1074/jbc.ra120.012933openalex-snapshot
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
inferred← citeshttps://doi.org/10.1080/10408347.2023.2197062openalex-snapshot
Recent progress in aptamer and CRISPR-Cas12a based systems for non-nucleic target detection
inferred← citeshttps://doi.org/10.1089/crispr.2021.0140openalex-snapshot
Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing
inferred← citeshttps://doi.org/10.1093/nar/gkac462openalex-snapshot
CRISPR-Cas12a targeting of ssDNA plays no detectable role in immunity
inferred← citeshttps://doi.org/10.1093/nar/gkac578openalex-snapshot
Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters
inferred← citeshttps://doi.org/10.1093/nar/gkac767openalex-snapshot
Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain
inferred← citeshttps://doi.org/10.1093/nar/gkad715openalex-snapshot
Unidirectional trans-cleaving behavior of CRISPR-Cas12a unlocks for an ultrasensitive assay using hybrid DNA reporters containing a 3′ toehold
inferred← citeshttps://doi.org/10.1093/nar/gkae152openalex-snapshot
Determinants of CRISPR Cas12a nuclease activation by DNA and RNA targets
inferred← citeshttps://doi.org/10.1093/nar/gkae176openalex-snapshot
An autocatalytic CRISPR-Cas amplification effect propelled by the LNA-modified split activators for DNA sensing
inferred← citeshttps://doi.org/10.1093/nar/gkaf1485openalex-snapshot
Nuc domain electrostatics drive the trans cleavage activity of CRISPR–Cas12a
inferred← citeshttps://doi.org/10.1093/nar/gkaf712openalex-snapshot
Unleashing high trans -substrate cleavage kinetics of Cas12a for nucleic acid diagnostics
inferred← citeshttps://doi.org/10.1093/nar/gkaf988openalex-snapshot
CRISPR-Cas12a REC2–Nuc interactions drive target-strand cleavage and constrain trans cleavage
inferred← citeshttps://doi.org/10.1093/nar/gkag276openalex-snapshot
Insights into spacer acquisition of the type V-A CRISPR–Cas system of Francisella novicida U112
inferred← citeshttps://doi.org/10.1093/nar/gkag748openalex-snapshot
RAPID: evaluation of Cas12a protospacer nicking and chimeric reporters for PAM-independent RNA and DNA diagnostics
inferred← citeshttps://doi.org/10.1101/657791openalex-snapshot
Pervasive off-target and double-stranded DNA nicking by CRISPR-Cas12a
inferred← citeshttps://doi.org/10.1109/embc40787.2023.10340759openalex-snapshot
RNA reporter based CRISPR/Cas12a biosensing platform for sensitive detection of circulating tumor DNA
inferred← citeshttps://doi.org/10.1111/jipb.13342openalex-snapshot
Cas12a‐based on‐site, rapid detection of genetically modified crops
inferred← citeshttps://doi.org/10.1128/spectrum.03260-22openalex-snapshot
Rapid SARS-CoV-2 Variants Enzymatic Detection (SAVED) by CRISPR-Cas12a
inferred← citeshttps://doi.org/10.1360/nso/20220067openalex-snapshot
Nucleases in gene-editing technologies: past and prologue
inferred← citeshttps://doi.org/10.3389/fpls.2021.722552openalex-snapshot
Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato
inferred← citeshttps://doi.org/10.3390/bios16030170openalex-snapshot
A One-Pot CRISPR/Cas12a-Based Platform for Contamination-Free Nucleic Acid Amplification Detection
inferred← citeshttps://doi.org/10.3390/ijms23020631openalex-snapshot
RNA-Guided AsCas12a- and SpCas9-Catalyzed Knockout and Homology Directed Repair of the Omega-1 Locus of the Human Blood Fluke, Schistosoma mansoni
inferred← citeshttps://doi.org/10.34133/2020/8051764openalex-snapshot
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