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Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/ece3.4387openalex-snapshot
Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia)
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Effects of sampling seasons and locations on fish environmental DNA metabarcoding in dam reservoirs
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Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks
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Monitoring vertebrate biodiversity of a protected coastal wetland using eDNA metabarcoding
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Identifying a breeding habitat of a critically endangered fish, Acheilognathus typus, in a natural river in Japan
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Two universal primer sets for species identification among vertebrates
inferredcites →https://doi.org/10.1007/s10201-016-0508-5openalex-snapshot
A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant
inferredcites →https://doi.org/10.1007/s10530-016-1203-5openalex-snapshot
Improving the containment of a freshwater invader using environmental DNA (eDNA) based monitoring
inferredcites →https://doi.org/10.1007/s12562-020-01461-xopenalex-snapshot
MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples
inferredcites →https://doi.org/10.1007/s12686-016-0550-yopenalex-snapshot
Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene
inferredcites →https://doi.org/10.1016/j.biocon.2014.11.025openalex-snapshot
Characterizing the distribution of an endangered salmonid using environmental DNA analysis
inferredcites →https://doi.org/10.1016/j.biocon.2014.11.038openalex-snapshot
Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms
inferredcites →https://doi.org/10.1016/j.biocon.2016.03.010openalex-snapshot
Comparison of environmental DNA metabarcoding and conventional fish survey methods in a river system
inferredcites →https://doi.org/10.1016/j.biocon.2017.09.015openalex-snapshot
Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna
inferredcites →https://doi.org/10.1016/j.scitotenv.2018.02.295openalex-snapshot
Degradation and dispersion limit environmental DNA detection of rare amphibians in wetlands: Increasing efficacy of sampling designs
inferredcites →https://doi.org/10.1016/j.ympev.2005.11.021openalex-snapshot
Complete nucleotide sequence of the mitochondrial genome of a Malagasy poison frog Mantella madagascariensis: Evolutionary implications on mitochondrial genomes of higher anuran groups
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Mitochondrial DNA diversification, molecular phylogeny, and biogeography of the primitive rhacophorid genus Buergeria in East Asia
inferredcites →https://doi.org/10.1016/j.ympev.2012.10.015openalex-snapshot
Phylogeny and historical demography of Cynops pyrrhogaster (Amphibia: Urodela): Taxonomic relationships and distributional changes associated with climatic oscillations
inferredcites →https://doi.org/10.1017/s1464793105006950openalex-snapshot
Freshwater biodiversity: importance, threats, status and conservation challenges
inferredcites →https://doi.org/10.1023/a:1009930313242openalex-snapshot
What is a healthy ecosystem?
inferredcites →https://doi.org/10.1038/nature10650openalex-snapshot
Additive threats from pathogens, climate and land-use change for global amphibian diversity
inferredcites →https://doi.org/10.1038/srep40368openalex-snapshot
Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
inferredcites →https://doi.org/10.1093/bioinformatics/btq461openalex-snapshot
Search and clustering orders of magnitude faster than BLAST
inferredcites →https://doi.org/10.1093/cz/zoab094openalex-snapshot
Development of an eDNA metabarcoding tool for surveying the world’s largest amphibian
inferredcites →https://doi.org/10.1093/molbev/msn031openalex-snapshot
Phylogeny, Recombination, and Mechanisms of Stepwise Mitochondrial Genome Reorganization in Mantellid Frogs from Madagascar
inferredcites →https://doi.org/10.1093/molbev/mst010openalex-snapshot
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
inferredcites →https://doi.org/10.1093/nar/gkq742openalex-snapshot
Natural and engineered nicking endonucleases--from cleavage mechanism to engineering of strand-specificity
inferredcites →https://doi.org/10.1093/nar/gkr732openalex-snapshot
ecoPrimers: inference of new DNA barcode markers from whole genome sequence analysis
inferredcites →https://doi.org/10.1093/nar/gks596openalex-snapshot
Primer3—new capabilities and interfaces
inferredcites →https://doi.org/10.1098/rsos.150088openalex-snapshot
MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
inferredcites →https://doi.org/10.1098/rspb.2002.2218openalex-snapshot
Biological identifications through DNA barcodes
inferredcites →https://doi.org/10.1111/1365-2656.13468openalex-snapshot
Validating eDNA measurements of the richness and abundance of anurans at a large scale
inferredcites →https://doi.org/10.1111/1365-2664.12392openalex-snapshot
A basin‐scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan
inferredcites →https://doi.org/10.1111/1755-0998.12159openalex-snapshot
Factors influencing detection of eDNA from a stream‐dwelling amphibian
inferredcites →https://doi.org/10.1111/1755-0998.12433openalex-snapshot
Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding
inferredcites →https://doi.org/10.1111/1755-0998.12643openalex-snapshot
eDNA metabarcoding: a promising method for anuran surveys in highly diverse tropical forests
inferredcites →https://doi.org/10.1111/1755-0998.12685openalex-snapshot
Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA
inferredcites →https://doi.org/10.1111/1755-0998.12690openalex-snapshot
Environmental DNA enables detection of terrestrial mammals from forest pond water
inferredcites →https://doi.org/10.1111/1755-0998.12934openalex-snapshot
Accuracy, limitations and cost efficiency of eDNA‐based community survey in tropical frogs
inferredcites →https://doi.org/10.1111/1755-0998.13200openalex-snapshot
Quantitative evaluation of intraspecific genetic diversity in a natural fish population using environmental DNA analysis
inferredcites →https://doi.org/10.1111/2041-210x.12595openalex-snapshot
Critical considerations for the application of environmental DNA methods to detect aquatic species
inferredcites →https://doi.org/10.1111/2041-210x.12743openalex-snapshot
Dealing with false‐positive and false‐negative errors about species occurrence at multiple levels
inferredcites →https://doi.org/10.1111/2041-210x.12849openalex-snapshot
Scrutinizing key steps for reliable metabarcoding of environmental samples
inferredcites →https://doi.org/10.1111/2041-210x.13276openalex-snapshot
Non‐specific amplification compromises environmental DNA metabarcoding with COI
inferredcites →https://doi.org/10.1111/2041-210x.13485openalex-snapshot
A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish
inferredcites →https://doi.org/10.1111/cobi.13183openalex-snapshot
Combined use of eDNA metabarcoding and video surveillance for the assessment of fish biodiversity
inferredcites →https://doi.org/10.1111/fwb.13800openalex-snapshot
Environmental DNA metabarcoding elucidates patterns of fish colonisation and co‐occurrences with amphibians in temperate wetlands created for biodiversity
inferredcites →https://doi.org/10.1111/j.1755-263x.2010.00158.xopenalex-snapshot
“Sight‐unseen” detection of rare aquatic species using environmental DNA
inferredcites →https://doi.org/10.1111/mec.13428openalex-snapshot
Next‐generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
inferredcites →https://doi.org/10.1111/mec.14350openalex-snapshot
Environmental DNA metabarcoding: Transforming how we survey animal and plant communities
inferredcites →https://doi.org/10.1111/mec.15742openalex-snapshot
Monitoring of spatiotemporal occupancy patterns of fish and amphibian species in a lentic aquatic system using environmental DNA
inferredcites →https://doi.org/10.1126/science.1103538openalex-snapshot
Status and Trends of Amphibian Declines and Extinctions Worldwide
inferredcites →https://doi.org/10.1126/science.1187512openalex-snapshot
Global Biodiversity: Indicators of Recent Declines
inferredcites →https://doi.org/10.1126/science.abd3369openalex-snapshot
Human impacts on global freshwater fish biodiversity
inferredcites →https://doi.org/10.1139/cjfas-2013-0047openalex-snapshot
Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples
inferredcites →https://doi.org/10.1139/gen-2016-0100openalex-snapshot
The importance of molecular markers and primer design when characterizing biodiversity from environmental DNA
inferredcites →https://doi.org/10.1146/annurev-marine-041421-082251openalex-snapshot
Environmental DNA Metabarcoding: A Novel Method for Biodiversity Monitoring of Marine Fish Communities
inferredcites →https://doi.org/10.1163/157075405774483139openalex-snapshot
Amphibian Research and Monitoring Initiative (ARMI): A successful start to a national program in the United States
inferredcites →https://doi.org/10.11646/zootaxa.3636.1.10openalex-snapshot
KUNIO SEKIYA, IKUO MIURA & MITSUAKI OGATA (2012) A new frog species of the genus Rugosa from Sado Island, Japan (Anura, Ranidae). Zootaxa, 3575, 49–62.
inferredcites →https://doi.org/10.1186/1471-2164-14-633openalex-snapshot
Afrobatrachian mitochondrial genomes: genome reorganization, gene rearrangement mechanisms, and evolutionary trends of duplicated and rearranged genes
inferredcites →https://doi.org/10.1186/1742-9994-2-1openalex-snapshot
Linking biogeography to physiology: Evolutionary and acclimatory adjustments of thermal limits
inferredcites →https://doi.org/10.1186/1742-9994-2-5openalex-snapshot
Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians
inferredcites →https://doi.org/10.1371/journal.pone.0035868openalex-snapshot
Estimation of Fish Biomass Using Environmental DNA
inferredcites →https://doi.org/10.1371/journal.pone.0076910openalex-snapshot
Two New Computational Methods for Universal DNA Barcoding: A Benchmark Using Barcode Sequences of Bacteria, Archaea, Animals, Fungi, and Land Plants
inferredcites →https://doi.org/10.1371/journal.pone.0191737openalex-snapshot
Seasonal variation in environmental DNA detection in sediment and water samples
inferredcites →https://doi.org/10.1371/journal.pone.0222493openalex-snapshot
Development of a quantitative polymerase chain reaction assay and environmental DNA sampling methods for Giant Gartersnake (Thamnophis gigas)
inferredcites →https://doi.org/10.18637/jss.v067.i01openalex-snapshot
Fitting Linear Mixed-Effects Models Using lme4
inferredcites →https://doi.org/10.2108/zs190007openalex-snapshot
A Phylogenetically Distinct Group of Glandirana rugosa Found in Kyushu, Japan
inferredcites →https://doi.org/10.3105/018.028.0101openalex-snapshot
PCR Primers for the Neobatrachian Mitochondrial Genome
inferredcites →https://doi.org/10.3390/d13090440openalex-snapshot
Application of eDNA Metabarcoding for Detecting Anura on a Tropical Island
inferredcites →https://doi.org/10.3791/61825openalex-snapshot
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
inferredcites →https://doi.org/10.7717/peerj.12357openalex-snapshot
Monitoring newt communities in urban area using eDNA metabarcoding
inferredcites →https://doi.org/10.7717/peerj.7335openalex-snapshot
The effects of spatial and temporal replicate sampling on eDNA metabarcoding

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← backgroundhttps://doi.org/10.3897/mbmg.6.76534.suppl1zenodo-depositor-declared
Supplementary material 1 from: Sakata MK, Kawata MU, Kurabayashi A, Kurita T, Nakamura M, Shirako T, Kakehashi R, Nishikawa K, Hossman MY, Nishijima T, Kabamoto J, Miya M, Minamoto T (2022) Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia.…
inferred← citeshttps://doi.org/10.1002/aqc.70174openalex-snapshot
A Degenerate Primer Set for eDNA Metabarcoding of Freshwater Amphibians, Turtles and Fish
inferred← citeshttps://doi.org/10.1002/aqc.70437openalex-snapshot
Optimising the Detection of Southern Toadlets ( Pseudophryne semimarmorata ): A Comparative Study of eDNA and Conventional Survey Methods
inferred← citeshttps://doi.org/10.1002/edn3.399openalex-snapshot
Monitoring the birds and the bees: Environmental DNA metabarcoding of flowers detects plant–animal interactions
inferred← citeshttps://doi.org/10.1002/edn3.588openalex-snapshot
Environmental DNA metabarcoding on aquatic insects: Comparing the primer sets of MtInsects‐16S based on the mtDNA 16S and general marker based on the mtDNA COI region
inferred← citeshttps://doi.org/10.1007/s10201-022-00710-5openalex-snapshot
Development of novel PCR primer sets for DNA barcoding of aquatic insects, and the discovery of some cryptic species
inferred← citeshttps://doi.org/10.1007/s10531-026-03280-5openalex-snapshot
Feasibility of an eDNA-based educational programme for high school students
inferred← citeshttps://doi.org/10.1007/s11252-026-02090-5openalex-snapshot
Anurans in culturally managed temple and shrine waterscapes in Kyoto: integrated visual, acoustic, and environmental DNA surveys
inferred← citeshttps://doi.org/10.1016/j.ab.2024.115618openalex-snapshot
Experimental study on the detection of Gastrodia elata by enzymatic recombinase amplification and immunochromatography
inferred← citeshttps://doi.org/10.1016/j.biocon.2024.110472openalex-snapshot
Public attention towards declining global amphibian species
inferred← citeshttps://doi.org/10.1016/j.cacint.2023.100101openalex-snapshot
Heterogeneous preference for biodiversity in Japanese urban blue spaces based on people's nature experiences: Analysis using eDNA and satisfaction data
inferred← citeshttps://doi.org/10.1016/j.ecolind.2025.113459openalex-snapshot
Application of eDNA metabarcoding to assess spatial distribution and habitat use by freshwater fish in a peri-alpine lake
inferred← citeshttps://doi.org/10.1016/j.gecco.2025.e04011openalex-snapshot
Amphibian diversity and distribution patterns in Chaohu Lake basin based on environmental DNA and traditional line transect method
inferred← citeshttps://doi.org/10.1016/j.marenvres.2025.107094openalex-snapshot
Development of universal PCR primers for the environmental DNA metabarcoding of cephalopod (Mollusca) diversity
inferred← citeshttps://doi.org/10.1039/9781837672004-00006openalex-snapshot
Considerations of Amphibian Models and Testing Paradigms for Ecological Risk Assessment
inferred← citeshttps://doi.org/10.1093/dnares/dsac018openalex-snapshot
Environmental DNA analysis for macro-organisms: species distribution and more
inferred← citeshttps://doi.org/10.1101/2022.08.02.502273openalex-snapshot
Seasonal eDNA-based monitoring of Batrachochytrium dendrobatidis and amphibian species in Norway
inferred← citeshttps://doi.org/10.1101/2023.06.29.547017openalex-snapshot
Definitive environmental DNA research on aquatic insects: Analysis optimization using the recently developed MtInsects-16S primers set
inferred← citeshttps://doi.org/10.1101/2025.09.02.672770openalex-snapshot
Feasibility of an eDNA-based educational program for high school students
inferred← citeshttps://doi.org/10.1111/1749-4877.12832openalex-snapshot
Validation and development of eDNA metabarcoding primers for comprehensive assessment of Chinese amphibians
inferred← citeshttps://doi.org/10.1111/1755-0998.13931openalex-snapshot
Enhancing amphibian biomonitoring through eDNA metabarcoding
inferred← citeshttps://doi.org/10.1111/1755-0998.14082openalex-snapshot
Navigating Methodological Trade‐Offs in eDNA Metabarcoding Biodiversity Monitoring: Insights From a Mediterranean Watershed
inferred← citeshttps://doi.org/10.1111/1755-0998.70122openalex-snapshot
A Field‐Deployable eDNA Metabarcoding Workflow Including De Novo Reference Assembly for Characterising Understudied Biodiversity Hotspots
inferred← citeshttps://doi.org/10.1111/ens.12616openalex-snapshot
Environmental DNA metabarcoding for insect biodiversity surveys: Current status and future prospects for potential applications in highly diverse insect taxa
inferred← citeshttps://doi.org/10.11606/t.11.2022.tde-24022023-121205openalex-snapshot
IInfluence of cropping systems on insect predators diversity and their trophic relationships
inferred← citeshttps://doi.org/10.1371/journal.pone.0290836openalex-snapshot
From roads to biobanks: Roadkill animals as a valuable source of genetic data
inferred← citeshttps://doi.org/10.18307/2025.0409openalex-snapshot
Research and application progress of environmental DNA technology in amphibian monitoring
inferred← citeshttps://doi.org/10.20944/preprints202312.1366.v1openalex-snapshot
Anura (Class Amphibia) Diversity Based on Environmental DNA (eDNA) in the Paddy Field Water Environment
inferred← citeshttps://doi.org/10.2108/zs250005openalex-snapshot
Fecal DNA Metabarcoding Analyses Imply Seasonally Opportunistic Feeding by the Japanese Marten, Martes melampus (Mammalia: Carnivora), in Southwestern Honshu Island, Japan
inferred← citeshttps://doi.org/10.21638/spbu03.2024.407openalex-snapshot
Aquatic environmental DNA: Applications for assessment and monitoring vertebrate
inferred← citeshttps://doi.org/10.3106/ms2024-0018openalex-snapshot
Diets of Rodents Revealed through DNA Metabarcoding
inferred← citeshttps://doi.org/10.3390/w16213063openalex-snapshot
Can eDNA Present in Aquatic Environments of Rural Areas Help Identify Species Diversity in the Order Anura?
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