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Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1002/ece3.2186openalex-snapshot
Estimating species richness using environmental DNA
inferredcites →https://doi.org/10.1002/ece3.4387openalex-snapshot
Toward an ecoregion scale evaluation of eDNA metabarcoding primers: A case study for the freshwater fish biodiversity of the Murray–Darling Basin (Australia)
inferredcites →https://doi.org/10.1002/ece3.4802openalex-snapshot
Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution
inferredcites →https://doi.org/10.1002/ece3.7658openalex-snapshot
Environmental DNA metabarcoding primers for freshwater fish detection and quantification: In silico and in tanks
inferredcites →https://doi.org/10.1002/edn3.12openalex-snapshot
Monitoring the eradication of the highly invasive topmouth gudgeon ( Pseudorasbora parva ) using a novel eDNA assay
inferredcites →https://doi.org/10.1007/s00414-006-0113-yopenalex-snapshot
Two universal primer sets for species identification among vertebrates
inferredcites →https://doi.org/10.1007/s12686-016-0550-yopenalex-snapshot
Freshwater vertebrate metabarcoding on Illumina platforms using double-indexed primers of the mitochondrial 16S rRNA gene
inferredcites →https://doi.org/10.1016/j.biocon.2014.11.019openalex-snapshot
Environmental DNA – An emerging tool in conservation for monitoring past and present biodiversity
inferredcites →https://doi.org/10.1016/j.biocon.2016.03.010openalex-snapshot
Comparison of environmental DNA metabarcoding and conventional fish survey methods in a river system
inferredcites →https://doi.org/10.1016/j.ecolind.2011.04.002openalex-snapshot
Integrative freshwater ecology and biodiversity conservation
inferredcites →https://doi.org/10.1016/j.fishres.2017.09.013openalex-snapshot
Freshwater fisheries assessment using environmental DNA: A primer on the method, its potential, and shortcomings as a conservation tool
inferredcites →https://doi.org/10.1016/j.jembe.2018.09.004openalex-snapshot
Species-specific detection and quantification of environmental DNA from marine fishes in the Baltic Sea
inferredcites →https://doi.org/10.1016/j.scitotenv.2018.05.002openalex-snapshot
The future of biotic indices in the ecogenomic era: Integrating (e)DNA metabarcoding in biological assessment of aquatic ecosystems
inferredcites →https://doi.org/10.1016/j.scitotenv.2018.08.370openalex-snapshot
eDNA as a tool for identifying freshwater species in sustainable forestry: A critical review and potential future applications
inferredcites →https://doi.org/10.1021/acs.est.5b00253openalex-snapshot
Droplet Digital Polymerase Chain Reaction (PCR) Outperforms Real-Time PCR in the Detection of Environmental DNA from an Invasive Fish Species
inferredcites →https://doi.org/10.1021/es404734popenalex-snapshot
Environmental Conditions Influence eDNA Persistence in Aquatic Systems
inferredcites →https://doi.org/10.1038/nature01547openalex-snapshot
Explaining the excess of rare species in natural species abundance distributions
inferredcites →https://doi.org/10.1038/nmeth.f.303openalex-snapshot
QIIME allows analysis of high-throughput community sequencing data
inferredcites →https://doi.org/10.1038/s41598-018-28424-8openalex-snapshot
Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation
inferredcites →https://doi.org/10.1038/srep22908openalex-snapshot
In silico assessment of primers for eDNA studies using PrimerTree and application to characterize the biodiversity surrounding the Cuyahoga River
inferredcites →https://doi.org/10.1080/03632415.2017.1276329openalex-snapshot
Comparative Cost and Effort of Fish Distribution Detection via Environmental DNA Analysis and Electrofishing
inferredcites →https://doi.org/10.1093/bioinformatics/btr381openalex-snapshot
UCHIME improves sensitivity and speed of chimera detection
inferredcites →https://doi.org/10.1093/bioinformatics/btr507openalex-snapshot
FLASH: fast length adjustment of short reads to improve genome assemblies
inferredcites →https://doi.org/10.1093/bioinformatics/btu170openalex-snapshot
Trimmomatic: a flexible trimmer for Illumina sequence data
inferredcites →https://doi.org/10.1093/bioinformatics/btu494openalex-snapshot
STAMP: statistical analysis of taxonomic and functional profiles
inferredcites →https://doi.org/10.1093/molbev/mst141openalex-snapshot
MitoFish and MitoAnnotator: A Mitochondrial Genome Database of Fish with an Accurate and Automatic Annotation Pipeline
inferredcites →https://doi.org/10.1093/nar/gkr732openalex-snapshot
ecoPrimers: inference of new DNA barcode markers from whole genome sequence analysis
inferredcites →https://doi.org/10.1093/oso/9780198767220.001.0001openalex-snapshot
Environmental DNA
inferredcites →https://doi.org/10.1098/rsbl.2014.0562openalex-snapshot
DNA metabarcoding and the cytochrome c oxidase subunit I marker: not a perfect match
inferredcites →https://doi.org/10.1098/rsos.150088openalex-snapshot
MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species
inferredcites →https://doi.org/10.1098/rspb.2019.1409openalex-snapshot
Predicting the fate of eDNA in the environment and implications for studying biodiversity
inferredcites →https://doi.org/10.1101/113472openalex-snapshot
Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing
inferredcites →https://doi.org/10.1111/1365-2664.13352openalex-snapshot
Ground‐truthing of a fish‐based environmental DNA metabarcoding method for assessing the quality of lakes
inferredcites →https://doi.org/10.1111/1755-0998.12285openalex-snapshot
Distance, flow and PCR inhibition: e DNA dynamics in two headwater streams
inferredcites →https://doi.org/10.1111/1755-0998.12433openalex-snapshot
Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding
inferredcites →https://doi.org/10.1111/1755-0998.12522openalex-snapshot
Estimating fish abundance and biomass from eDNA concentrations: variability among capture methods and environmental conditions
inferredcites →https://doi.org/10.1111/1755-0998.12685openalex-snapshot
Rapid degradation of longer DNA fragments enables the improved estimation of distribution and biomass using environmental DNA
inferredcites →https://doi.org/10.1111/1755-0998.12900openalex-snapshot
Unlocking biodiversity and conservation studies in high‐diversity environments using environmental DNA (eDNA): A test with Guianese freshwater fishes
inferredcites →https://doi.org/10.1111/2041-210x.12598openalex-snapshot
Assessing the cost‐efficiency of environmental DNA sampling
inferredcites →https://doi.org/10.1111/2041-210x.12687openalex-snapshot
PrimerMiner : an r package for development and in silico validation of DNA metabarcoding primers
inferredcites →https://doi.org/10.1111/2041-210x.13276openalex-snapshot
Non‐specific amplification compromises environmental DNA metabarcoding with COI
inferredcites →https://doi.org/10.1111/2041-210x.13485openalex-snapshot
A comprehensive and comparative evaluation of primers for metabarcoding eDNA from fish
inferredcites →https://doi.org/10.1111/brv.12480openalex-snapshot
Emerging threats and persistent conservation challenges for freshwater biodiversity
inferredcites →https://doi.org/10.1111/faf.12286openalex-snapshot
The sceptical optimist: challenges and perspectives for the application of environmental DNA in marine fisheries
inferredcites →https://doi.org/10.1111/fwb.13094openalex-snapshot
Comparing local‐ and regional‐scale estimations of the diversity of stream fish using eDNA metabarcoding and conventional observation methods
inferredcites →https://doi.org/10.1111/geb.12096openalex-snapshot
Global patterns of freshwater species diversity, threat and endemism
inferredcites →https://doi.org/10.1111/j.1365-294x.2012.05542.xopenalex-snapshot
Environmental DNA
inferredcites →https://doi.org/10.1111/jfb.14177openalex-snapshot
Environmental DNA is not the tool by itself
inferredcites →https://doi.org/10.1111/mec.13428openalex-snapshot
Next‐generation monitoring of aquatic biodiversity using environmental DNA metabarcoding
inferredcites →https://doi.org/10.1111/mec.13660openalex-snapshot
Environmental DNA metabarcoding of lake fish communities reflects long‐term data from established survey methods
inferredcites →https://doi.org/10.1139/cjfas-2015-0262openalex-snapshot
Active and passive environmental DNA surveillance of aquatic invasive species
inferredcites →https://doi.org/10.1139/cjfas-2016-0306openalex-snapshot
Fish community assessment with eDNA metabarcoding: effects of sampling design and bioinformatic filtering
inferredcites →https://doi.org/10.1139/cjfas-2017-0114openalex-snapshot
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations
inferredcites →https://doi.org/10.1186/1471-2105-10-421openalex-snapshot
BLAST+: architecture and applications
inferredcites →https://doi.org/10.1186/1471-2180-10-189openalex-snapshot
ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases
inferredcites →https://doi.org/10.1371/journal.pcbi.1000352openalex-snapshot
Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples
inferredcites →https://doi.org/10.1371/journal.pone.0086175openalex-snapshot
Using Environmental DNA to Census Marine Fishes in a Large Mesocosm
inferredcites →https://doi.org/10.1371/journal.pone.0088786openalex-snapshot
Transport Distance of Invertebrate Environmental DNA in a Natural River
inferredcites →https://doi.org/10.1371/journal.pone.0114639openalex-snapshot
The Release Rate of Environmental DNA from Juvenile and Adult Fish
inferredcites →https://doi.org/10.1371/journal.pone.0130324openalex-snapshot
Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass—Sequence Relationships with an Innovative Metabarcoding Protocol
inferredcites →https://doi.org/10.1371/journal.pone.0165252openalex-snapshot
Environmental DNA from Seawater Samples Correlate with Trawl Catches of Subarctic, Deepwater Fishes
inferredcites →https://doi.org/10.1577/1548-8659(2001)130<0620:aatepo>2.0.co;2openalex-snapshot
An Approach to Estimate Probability of Presence and Richness of Fish Species
inferredcites →https://doi.org/10.1899/08-171.1openalex-snapshot
Freshwater biodiversity conservation: recent progress and future challenges
inferredcites →https://doi.org/10.3390/genes11030296openalex-snapshot
Standards for Methods Utilizing Environmental DNA for Detection of Fish Species
inferredcites →https://doi.org/10.3391/mbi.2014.5.3.05openalex-snapshot
Tracking ghosts: combined electrofishing and environmental DNA surveillance efforts for Asian carps in Ontario waters of Lake Erie
inferredcites →https://doi.org/10.3391/mbi.2017.8.3.08openalex-snapshot
Experimental observations on the decay of environmental DNA from bighead and silver carps
inferredcites →https://doi.org/10.3897/mbmg.2.24262openalex-snapshot
Estimating fish alpha- and beta-diversity along a small stream with environmental DNA metabarcoding
inferredcites →https://doi.org/10.47886/9781934874103.fmatteropenalex-snapshot
Standard Methods for Sampling North American Freshwater Fishes

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.3897/mbmg.8.128646zenodo-depositor-declared
Benchmarking the discrimination power of commonly used markers and amplicons in marine fish (e)DNA (meta)barcoding
inferred← citeshttps://doi.org/10.1002/ece3.10681openalex-snapshot
Diversity and spatiotemporal dynamics of fish communities in the Chongqing section of the upper Yangtze River based on eDNA metabarcoding
inferred← citeshttps://doi.org/10.1002/ece3.11214openalex-snapshot
Application of environmental DNA metabarcoding to identify fish community characteristics in subtropical river systems
inferred← citeshttps://doi.org/10.1002/ece3.11631openalex-snapshot
Experimental evaluation of genetic variability based on DNA metabarcoding from the aquatic environment: Insights from the Leray COI fragment
inferred← citeshttps://doi.org/10.1002/edn3.387openalex-snapshot
Small changes make big progress: A more efficient eDNA monitoring method for freshwater fish
inferred← citeshttps://doi.org/10.1002/edn3.393openalex-snapshot
Using environmental DNA to investigate avian interactions with flowering plants
inferred← citeshttps://doi.org/10.1002/edn3.413openalex-snapshot
eDNA metabarcoding from aquatic biofilms allows studying spatial and temporal fluctuations of fish communities from Lake Geneva
inferred← citeshttps://doi.org/10.1002/edn3.554openalex-snapshot
Primer set evaluation and sampling method assessment for the monitoring of fish communities in the North‐western part of the Mediterranean Sea through eDNA metabarcoding
inferred← citeshttps://doi.org/10.1002/edn3.70095openalex-snapshot
eDNA Replicates, Polymerase and Amplicon Size Impact Inference of Richness Across Habitats
inferred← citeshttps://doi.org/10.1002/etc.5696openalex-snapshot
Cryptic Species in Ecotoxicology
inferred← citeshttps://doi.org/10.1007/978-981-19-4162-7_31openalex-snapshot
Fish Classification System Using Customized Deep Residual Neural Networks on Small-Scale Underwater Images
inferred← citeshttps://doi.org/10.1007/s00300-025-03345-1openalex-snapshot
A study of taxonomic and molecular-genetic characteristics of the western Chukchi Sea fish fauna
inferred← citeshttps://doi.org/10.1007/s00343-026-5439-3openalex-snapshot
Comparative evaluation of fish community diversity and stability in the Zhanjiang mangrove watershed using environmental DNA and centipede net sampling
inferred← citeshttps://doi.org/10.1007/s10750-025-05826-0openalex-snapshot
A comparison of environmental DNA sampling strategies for detecting anuran populations in wetlands
inferred← citeshttps://doi.org/10.1007/s11160-026-10040-9openalex-snapshot
Fish diversity assessment through environmental DNA metabarcoding: a synthesis review of mock community studies
inferred← citeshttps://doi.org/10.1007/s42991-025-00489-9openalex-snapshot
Pioneering DNA metabarcoding analysis of the African wolf (Canis lupaster) diet
inferred← citeshttps://doi.org/10.1016/j.avrs.2025.100268openalex-snapshot
Multi-dimensional niche segregation of sympatric breeding seabirds in the Xisha Archipelago
inferred← citeshttps://doi.org/10.1016/j.crfs.2026.101486openalex-snapshot
Refinement and application of 12S rRNA meta-barcoding primers for seafood identification in multispecies product
inferred← citeshttps://doi.org/10.1016/j.ecolind.2023.111111openalex-snapshot
Unraveling fish diversity and assembly patterns in a temperate river: Evidence from environmental DNA metabarcoding and morphological data
inferred← citeshttps://doi.org/10.1016/j.envint.2023.107950openalex-snapshot
Unsupervised biological integrity assessment by eDNA biomonitoring of multi-trophic aquatic taxa
inferred← citeshttps://doi.org/10.1016/j.fishres.2024.107192openalex-snapshot
Environmental DNA reveals spatial and temporal variation in fish communities before the 10-year fishing ban in the Poyang Lake Basin
inferred← citeshttps://doi.org/10.1016/j.foodcont.2026.112127openalex-snapshot
Surimi species authentication using Nanopore metabarcoding with short- and long-amplicon 16S and COI primers
inferred← citeshttps://doi.org/10.1016/j.gecco.2025.e03906openalex-snapshot
Evaluation and application of eDNA metabarcoding primers of fish: A case study in an estuarine ecosystem
inferred← citeshttps://doi.org/10.1016/j.jglr.2024.102317openalex-snapshot
Nearshore fish diversity changes with sampling method and human disturbance: Comparing eDNA metabarcoding and seine netting along the Upper St. Lawrence River
inferred← citeshttps://doi.org/10.1016/j.limno.2022.125955openalex-snapshot
Characteristics of diurnal and seasonal changes in fish detection patterns using environmental DNA metabarcoding in a mountain stream
inferred← citeshttps://doi.org/10.1016/j.mex.2024.103020openalex-snapshot
A simple and cost-effective extraction for piscine environmental DNA metabarcoding using guanidine hydrochloride method
inferred← citeshttps://doi.org/10.1016/j.sciaf.2025.e02544openalex-snapshot
Environmental DNA metabarcoding reveals the presence of invasive and cryptic species in the Mweru-Luapula fishery
inferred← citeshttps://doi.org/10.1016/j.watbs.2022.100007openalex-snapshot
Methodology for fish biodiversity monitoring with environmental DNA metabarcoding: The primers, databases and bioinformatic pipelines
inferred← citeshttps://doi.org/10.1016/j.wsee.2024.08.003openalex-snapshot
Comparing the performance variability of different eDNA primers in fish monitoring
inferred← citeshttps://doi.org/10.1021/acsestwater.6c00425openalex-snapshot
Optimizing eDNA Detection in Fish Communities in Impounded Lakes: An Integrated Identification Approach
inferred← citeshttps://doi.org/10.1071/pc21078openalex-snapshot
Assessing freshwater fish biodiversity of Kumbe River, Papua (Indonesia) through environmental DNA metabarcoding
inferred← citeshttps://doi.org/10.1080/02705060.2025.2541689openalex-snapshot
Evaluating eDNA metabarcoding for fish biodiversity assessment in Nigerian aquatic ecosystems: potential, limitations, and comparisons with traditional methods
inferred← citeshttps://doi.org/10.1080/02705060.2026.2657359openalex-snapshot
Genetic characterization of freshwater fishes using DNA barcoding with 12S rRNA gene from rivers of Malakand division, Pakistan
inferred← citeshttps://doi.org/10.1080/15627020.2023.2274334openalex-snapshot
The status of COI and 12S rRNA DNA barcode reference libraries for freshwater fish in South Africa: Implications for future eDNA projects
inferred← citeshttps://doi.org/10.1111/1749-4877.12832openalex-snapshot
Validation and development of eDNA metabarcoding primers for comprehensive assessment of Chinese amphibians
inferred← citeshttps://doi.org/10.1111/1755-0998.70069openalex-snapshot
Benchmarking the Taxonomic Resolution of Fish eDNA Metabarcodes Against COI Barcodes
inferred← citeshttps://doi.org/10.1111/aje.13233openalex-snapshot
An assessment of South African small mammal barcode sequence libraries: Implications for future carnivore diet analyses by DNA
inferred← citeshttps://doi.org/10.1111/mec.16659openalex-snapshot
Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward
inferred← citeshttps://doi.org/10.1139/cjfas-2023-0227openalex-snapshot
Monitoring estuarine fish communities: environmental DNA (eDNA) metabarcoding as a complement to beach seining
inferred← citeshttps://doi.org/10.1146/annurev-marine-041421-082251openalex-snapshot
Environmental DNA Metabarcoding: A Novel Method for Biodiversity Monitoring of Marine Fish Communities
inferred← citeshttps://doi.org/10.13057/biodiv/d250242openalex-snapshot
Combining eDNA metabarcoding and conventional method in rapid screening of fish diversity in Maros-Pangkep Global Geopark, South Sulawesi, Indonesia
inferred← citeshttps://doi.org/10.1371/journal.pone.0266720openalex-snapshot
Comparing eDNA metabarcoding primers for assessing fish communities in a biodiverse estuary
inferred← citeshttps://doi.org/10.1371/journal.pone.0271400openalex-snapshot
Towards a DNA barcode library for Madagascar’s threatened ichthyofauna
inferred← citeshttps://doi.org/10.15517/hp29s516openalex-snapshot
Environmental DNA exploration of fish diversity in the Balantak River, Central Sulawesi, Indonesia
inferred← citeshttps://doi.org/10.18307/2024.0632openalex-snapshot
Effects of storage temperature and time of sampling and pre-treatment on fish biomonitoring through environmental DNA
inferred← citeshttps://doi.org/10.22541/au.163490255.53353112/v1openalex-snapshot
Estimation accuracy of species abundance based on environmental DNA with relation to its production source, state, and assay methodology suggested by meta-analyses
inferred← citeshttps://doi.org/10.22541/au.166756789.99942137/v1openalex-snapshot
Experimental evaluation of species and genetic variability based on DNA metabarcoding from the aquatic environment: extra OTUs formed by NUMTS may reduce the diversity of ASVs
inferred← citeshttps://doi.org/10.3390/ani16060871openalex-snapshot
Fish Diversity and Functional Traits in the Seagrass Based on the Environmental DNA Metabarcoding in the Li’an Bay, China
inferred← citeshttps://doi.org/10.3390/d18090548openalex-snapshot
Multi-Year eDNA Metabarcoding Reveals Fish Community Dynamics in the Jiangsu Section of the Yangtze River Mainstem Under the Fishing Ban
inferred← citeshttps://doi.org/10.3390/environments13070374openalex-snapshot
Complementary eDNA Markers Reveal Fish Biodiversity Patterns Across Environmental Gradients in a High-Andean River System
inferred← citeshttps://doi.org/10.3390/fishes10080375openalex-snapshot
Environmental DNA Metabarcoding as a Tool for Fast Fish Assessment in Post-Cleanup Activities: Example from Two Urban Lakes in Zagreb, Croatia
inferred← citeshttps://doi.org/10.3390/fishes11050264openalex-snapshot
Building a Local Multi-Marker eDNA Reference Database Reveals the Limitations of Public Repositories for Freshwater Fish Monitoring in the Three Gorges Reservoir
inferred← citeshttps://doi.org/10.3390/fishes9030086openalex-snapshot
Assessment of Fish Species in Wanlv Lake, the Largest Drinking Water Source in South China, by Environmental DNA Metabarcoding Technology
inferred← citeshttps://doi.org/10.3390/fishes9060192openalex-snapshot
Exploring Seasonal Variations in Fish Communities: A Study of the Yellow River Estuary and Its Adjacent Waters Using eDNA and Trawl Surveys
inferred← citeshttps://doi.org/10.3390/jmse13010060openalex-snapshot
Unveiling the UNESCO Biosphere Reserve of the Berlengas Archipelago in Portugal as a Hotspot of Fish Species Using eDNA Metabarcoding and the Collaboration of Fishing Crews
inferred← citeshttps://doi.org/10.3390/su15129497openalex-snapshot
Detection and Management of Freshwater Invasive Alien Species through Environmental DNA Analysis and Geographic Information Systems: A Review
inferred← citeshttps://doi.org/10.3390/w16050631openalex-snapshot
A Comparative Evaluation of eDNA Metabarcoding Primers in Fish Community Monitoring in the East Lake
inferred← citeshttps://doi.org/10.3390/w17020246openalex-snapshot
Assessment of Aquatic Ecosystem Health in the Irtysh River Basin Using eDNA Metabarcoding
inferred← citeshttps://doi.org/10.3897/mbmg.6.76534openalex-snapshot
Development and evaluation of PCR primers for environmental DNA (eDNA) metabarcoding of Amphibia
inferred← citeshttps://doi.org/10.3897/mbmg.7.103856openalex-snapshot
Evaluating five primer pairs for environmental DNA metabarcoding of Central European fish species based on mock communities
inferred← citeshttps://doi.org/10.5194/we-24-59-2024openalex-snapshot
Environmental DNA in different media reveals distribution characteristics and assembly mechanisms of fish assemblages in a complex river–lake system
inferred← citeshttps://doi.org/10.54612/a.5o01b216mlopenalex-snapshot
Provtagningsmetoder av akvatiska resurser med 3R i fokus
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