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A single-cell RNA-sequencing training and analysis suite using the Galaxy framework

https://doi.org/10.1093/gigascience/giaa102
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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Nature, Nurture, or Chance: Stochastic Gene Expression and Its Consequences
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Practical Computational Reproducibility in the Life Sciences
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Community-Driven Data Analysis Training for Biology
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Methods and challenges in the analysis of single-cell RNA-sequencing data
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Direct Comparative Analyses of 10X Genomics Chromium and Smart-Seq2
inferredcites →https://doi.org/10.1038/550451aopenalex-snapshot
The Human Cell Atlas: from vision to reality
inferredcites →https://doi.org/10.1038/nature14966openalex-snapshot
Single-cell messenger RNA sequencing reveals rare intestinal cell types
inferredcites →https://doi.org/10.1038/nbt.3192openalex-snapshot
Spatial reconstruction of single-cell gene expression data
inferredcites →https://doi.org/10.1038/nbt.3711openalex-snapshot
Revealing the vectors of cellular identity with single-cell genomics
inferredcites →https://doi.org/10.1038/ncomms13182openalex-snapshot
Massive and parallel expression profiling using microarrayed single-cell sequencing
inferredcites →https://doi.org/10.1038/ncomms14049openalex-snapshot
Massively parallel digital transcriptional profiling of single cells
inferredcites →https://doi.org/10.1038/nmeth.3174openalex-snapshot
bam.iobio: a web-based, real-time, sequence alignment file inspector
inferredcites →https://doi.org/10.1038/nmeth.4197openalex-snapshot
Salmon provides fast and bias-aware quantification of transcript expression
inferredcites →https://doi.org/10.1038/nmeth.4644openalex-snapshot
scmap: projection of single-cell RNA-seq data across data sets
inferredcites →https://doi.org/10.1038/nmeth.4662openalex-snapshot
FateID infers cell fate bias in multipotent progenitors from single-cell RNA-seq data
inferredcites →https://doi.org/10.1038/s41467-016-0009-6openalex-snapshot
In situ click chemistry generation of cyclooxygenase-2 inhibitors
inferredcites →https://doi.org/10.1038/s41586-019-0969-xopenalex-snapshot
The single-cell transcriptional landscape of mammalian organogenesis
inferredcites →https://doi.org/10.1038/s41592-018-0046-7openalex-snapshot
Bioconda: sustainable and comprehensive software distribution for the life sciences
inferredcites →https://doi.org/10.1038/s41592-019-0535-3openalex-snapshot
Supervised classification enables rapid annotation of cell atlases
inferredcites →https://doi.org/10.1038/s41592-019-0654-xopenalex-snapshot
Orchestrating single-cell analysis with Bioconductor
inferredcites →https://doi.org/10.1038/s41592-021-01102-wopenalex-snapshot
User-friendly, scalable tools and workflows for single-cell RNA-seq analysis
inferredcites →https://doi.org/10.1088/1742-5468/2008/10/p10008openalex-snapshot
Fast unfolding of communities in large networks
inferredcites →https://doi.org/10.1093/bioinformatics/bts635openalex-snapshot
STAR: ultrafast universal RNA-seq aligner
inferredcites →https://doi.org/10.1093/bioinformatics/btw777openalex-snapshot
Scater: pre-processing, quality control, normalization and visualization of single-cell RNA-seq data in R
inferredcites →https://doi.org/10.1093/bioinformatics/bty711openalex-snapshot
WIlsON: Web-based Interactive Omics VisualizatioN
inferredcites →https://doi.org/10.1093/gigascience/giz144openalex-snapshot
A Galaxy-based training resource for single-cell RNA-sequencing quality control and analyses
inferredcites →https://doi.org/10.1093/nar/gky379openalex-snapshot
The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update
inferredcites →https://doi.org/10.1101/009944openalex-snapshot
Phinch: An interactive, exploratory data visualization framework for –Omic datasets
inferredcites →https://doi.org/10.1101/gr.209601.116openalex-snapshot
UMI-tools: modeling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy
inferredcites →https://doi.org/10.1111/2041-210x.12945openalex-snapshot
Wallace : A flexible platform for reproducible modeling of species niches and distributions built for community expansion
inferredcites →https://doi.org/10.1126/science.aar5780openalex-snapshot
The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution
inferredcites →https://doi.org/10.1186/s13059-016-0938-8openalex-snapshot
CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq
inferredcites →https://doi.org/10.1186/s13059-016-0947-7openalex-snapshot
Pooling across cells to normalize single-cell RNA sequencing data with many zero counts
inferredcites →https://doi.org/10.1186/s13059-017-1381-1openalex-snapshot
RNA virus interference via CRISPR/Cas13a system in plants
inferredcites →https://doi.org/10.1186/s13059-017-1382-0openalex-snapshot
SCANPY: large-scale single-cell gene expression data analysis
inferredcites →https://doi.org/10.1186/s13059-018-1486-1openalex-snapshot
HiGlass: web-based visual exploration and analysis of genome interaction maps
inferredcites →https://doi.org/10.1186/s13059-019-1663-xopenalex-snapshot
PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells
inferredcites →https://doi.org/10.1186/s13059-019-1670-yopenalex-snapshot
Alevin efficiently estimates accurate gene abundances from dscRNA-seq data
inferredcites →https://doi.org/10.1186/s13059-019-1862-5openalex-snapshot
scPred: accurate supervised method for cell-type classification from single-cell RNA-seq data
inferredcites →https://doi.org/10.1371/journal.pcbi.1006361openalex-snapshot
scPipe: A flexible R/Bioconductor preprocessing pipeline for single-cell RNA-sequencing data
inferredcites →https://doi.org/10.15252/msb.20188746openalex-snapshot
Current best practices in single‐cell RNA‐seq analysis: a tutorial
inferredcites →https://doi.org/10.18129/b9.bioc.singlecellexperimentopenalex-snapshot
SingleCellExperiment
inferredcites →https://doi.org/10.21105/joss.00861openalex-snapshot
UMAP: Uniform Manifold Approximation and Projection
inferredcites →https://doi.org/10.3233/978-1-61499-649-1-87openalex-snapshot
Jupyter Notebooks – a publishing format for reproducible computational workflows
inferredcites →https://doi.org/10.48550/arxiv.1802.03426openalex-snapshot
UMAP: Uniform Manifold Approximation and Projection for Dimension Reduction
inferredcites →https://doi.org/10.5167/uzh-162597openalex-snapshot
The Human Cell Atlas
inferredcites →https://doi.org/10.5281/zenodo.3235020openalex-snapshot
chanzuckerberg/cellxgene: Release 0.10.0
inferredcites →https://doi.org/10.6084/m9.figshare.951915openalex-snapshot
Phinch: An interactive, exploratory data visualization framework for metagenomic datasets
inferredcites →https://doi.org/10.7554/elife.27041openalex-snapshot
The Human Cell Atlas

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.2139/ssrn.4132724openalex-extracted
Delayed Booster Dosing Improves Human Antigen-Specific IG and B Cell Responses to the RH5.1/AS01 B Malaria Vaccine
inferred← citeshttps://doi.org/10.1002/cpz1.31openalex-snapshot
Using Galaxy to Perform Large‐Scale Interactive Data Analyses—An Update
inferred← citeshttps://doi.org/10.1016/bs.apcsb.2022.11.015openalex-snapshot
A compilation of bioinformatic approaches to identify novel downstream targets for the detection and prophylaxis of cancer
inferred← citeshttps://doi.org/10.1016/j.ccell.2021.07.004openalex-snapshot
From bench to bedside: Single-cell analysis for cancer immunotherapy
inferred← citeshttps://doi.org/10.1016/j.xgen.2025.101005openalex-snapshot
Galaxy single-cell & spatial omics community update: Navigating new frontiers in 2025
inferred← citeshttps://doi.org/10.1038/s41592-021-01102-wopenalex-snapshot
User-friendly, scalable tools and workflows for single-cell RNA-seq analysis
inferred← citeshttps://doi.org/10.1080/14789450.2023.2265062openalex-snapshot
A Galaxy of informatics resources for MS-based proteomics
inferred← citeshttps://doi.org/10.1093/gigascience/giad091openalex-snapshot
Cellsnake: a user-friendly tool for single-cell RNA sequencing analysis
inferred← citeshttps://doi.org/10.1093/nargab/lqae134openalex-snapshot
scDAPP: a comprehensive single-cell transcriptomics analysis pipeline optimized for cross-group comparison
inferred← citeshttps://doi.org/10.1093/plphys/kiac468openalex-snapshot
Toward a data infrastructure for the Plant Cell Atlas
inferred← citeshttps://doi.org/10.1101/2020.04.15.043737openalex-snapshot
UNCURL-App: Interactive database-driven analysis of scRNA-Seq data
inferred← citeshttps://doi.org/10.1101/2021.08.16.456499openalex-snapshot
scFlow: A Scalable and Reproducible Analysis Pipeline for Single-Cell RNA Sequencing Data
inferred← citeshttps://doi.org/10.1101/2022.11.24.517172openalex-snapshot
Inhibition of myeloperoxidase attenuates thoracic aortic aneurysm formation in Marfan disease
inferred← citeshttps://doi.org/10.1145/3638243openalex-snapshot
Building Domain-Specific Machine Learning Workflows: A Conceptual Framework for the State of the Practice
inferred← citeshttps://doi.org/10.1146/annurev-animal-111523-102217openalex-snapshot
Comparative Genomics and Epigenomics of Transcriptional Regulation
inferred← citeshttps://doi.org/10.1172/jci.insight.163859openalex-snapshot
Delayed boosting improves human antigen-specific Ig and B cell responses to the RH5.1/AS01B malaria vaccine
inferred← citeshttps://doi.org/10.1371/journal.pcbi.1010752openalex-snapshot
Galaxy Training: A powerful framework for teaching!
inferred← citeshttps://doi.org/10.3389/fgene.2024.1460351openalex-snapshot
Building a FAIR data ecosystem for incorporating single-cell transcriptomics data into agricultural genome to phenome research
inferred← citeshttps://doi.org/10.64898/2026.06.11.731578openalex-snapshot
oxo-flow: compiled, memory-safe bioinformatics workflow orchestration
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