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Development and Validation of an Anion Exchange High-Performance Liquid Chromatography Method for Analysis of Empty Capsids and Capsids Encapsidating Genetic Material in a Purified Preparation of Recombinant Adeno-Associated Virus Serotype 5

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Outgoing claims: the papers this work cites, builds on, or argues with.

inferredcites →https://doi.org/10.1016/bs.mie.2015.05.001openalex-snapshot
Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data
inferredcites →https://doi.org/10.1016/j.ab.2016.12.017openalex-snapshot
Characterization of charge variants of a monoclonal antibody using weak anion exchange chromatography at subunit levels
inferredcites →https://doi.org/10.1016/j.chroma.2015.06.015openalex-snapshot
Rapid analysis of charge variants of monoclonal antibodies using non-linear salt gradient in cation-exchange high performance liquid chromatography
inferredcites →https://doi.org/10.1016/j.jchromb.2017.02.017openalex-snapshot
Charge variants characterization of a monoclonal antibody by ion exchange chromatography coupled on-line to native mass spectrometry: Case study after a long-term storage at +5 °C
inferredcites →https://doi.org/10.1016/j.jviromet.2006.11.019openalex-snapshot
Separation of adeno-associated virus type 2 empty particles from genome containing vectors by anion-exchange column chromatography
inferredcites →https://doi.org/10.1016/j.jviromet.2013.10.038openalex-snapshot
Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
inferredcites →https://doi.org/10.1016/j.omtm.2019.02.003openalex-snapshot
Achieving High-Yield Production of Functional AAV5 Gene Delivery Vectors via Fedbatch in an Insect Cell-One Baculovirus System
inferredcites →https://doi.org/10.1016/j.omtm.2019.09.006openalex-snapshot
Developing an Anion Exchange Chromatography Assay for Determining Empty and Full Capsid Contents in AAV6.2
inferredcites →https://doi.org/10.1016/j.ymthe.2005.11.024openalex-snapshot
Removal of Empty Capsids from Type 1 Adeno-Associated Virus Vector Stocks by Anion-Exchange Chromatography Potentiates Transgene Expression
inferredcites →https://doi.org/10.1016/j.ymthe.2017.08.003openalex-snapshot
Direct Head-to-Head Evaluation of Recombinant Adeno-associated Viral Vectors Manufactured in Human versus Insect Cells
inferredcites →https://doi.org/10.1016/s1525-0016(02)00019-9openalex-snapshot
Quantification of adeno-associated virus particles and empty capsids by optical density measurement
inferredcites →https://doi.org/10.1021/acs.analchem.6b00883openalex-snapshot
Resolving Adeno-Associated Viral Particle Diversity With Charge Detection Mass Spectrometry
inferredcites →https://doi.org/10.1021/ja102800ropenalex-snapshot
Fluorescence of Natural DNA: From the Femtosecond to the Nanosecond Time Scales
inferredcites →https://doi.org/10.1021/jp026063gopenalex-snapshot
Fluorescence Properties of DNA Nucleosides and Nucleotides: A Refined Steady-State and Femtosecond Investigation
inferredcites →https://doi.org/10.1038/gt.2010.27openalex-snapshot
Near-perfect infectivity of wild-type AAV as benchmark for infectivity of recombinant AAV vectors
inferredcites →https://doi.org/10.1038/mt.2012.194openalex-snapshot
Endgame: Glybera Finally Recommended for Approval as the First Gene Therapy Drug in the European Union
inferredcites →https://doi.org/10.1038/mt.2013.268openalex-snapshot
AAV Empty Capsids: For Better or for Worse?
inferredcites →https://doi.org/10.1038/s41573-019-0012-9openalex-snapshot
Adeno-associated virus vector as a platform for gene therapy delivery
inferredcites →https://doi.org/10.1038/sj.gt.3300946openalex-snapshot
Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant AAV-2
inferredcites →https://doi.org/10.1089/hgtb.2011.217openalex-snapshot
Analysis of Particle Content of Recombinant Adeno-Associated Virus Serotype 8 Vectors by Ion-Exchange Chromatography
inferredcites →https://doi.org/10.1089/hgtb.2015.048openalex-snapshot
Analytical Ultracentrifugation as an Approach to Characterize Recombinant Adeno-Associated Viral Vectors
inferredcites →https://doi.org/10.1089/hgtb.2019.088openalex-snapshot
Analytical Strategies for Quantification of Adeno-Associated Virus Empty Capsids to Support Process Development
inferredcites →https://doi.org/10.1089/hum.2017.29039.trfopenalex-snapshot
Empty Adeno-Associated Virus Capsids: Contaminant or Natural Decoy?
inferredcites →https://doi.org/10.1089/hum.2019.041openalex-snapshot
Filling Adeno-Associated Virus Capsids: Estimating Success by Cryo-Electron Microscopy
inferredcites →https://doi.org/10.1093/embo-reports/kve234openalex-snapshot
Electron cryo‐microscopy and image reconstruction of adeno‐associated virus type 2 empty capsids
inferredcites →https://doi.org/10.2174/156652307782151425openalex-snapshot
Immune Responses to AAV in Clinical Trials
inferredcites →https://doi.org/10.2174/156652311796150354openalex-snapshot
Immune Responses to AAV in Clinical Trials
inferredcites →https://doi.org/10.3390/biomedicines2010080openalex-snapshot
Product-Related Impurities in Clinical-Grade Recombinant AAV Vectors: Characterization and Risk Assessment
inferredcites →https://doi.org/10.3390/ijms151222518openalex-snapshot
Intrinsic Tryptophan Fluorescence in the Detection and Analysis of Proteins: A Focus on Förster Resonance Energy Transfer Techniques

What points at this work

Incoming claims: where this work appears in other papers’ reference lists and arguments.

inferred← citeshttps://doi.org/10.2139/ssrn.4592842openalex-extracted
Separation of Full and Empty AAV Capsids by Anion-Exchange Chromatography Using Choline-Type Salts
inferred← citeshttps://doi.org/10.1007/978-3-031-28489-2_13openalex-snapshot
Process Analytical Technologies (PAT) and Quality by Design (QbD) for Bioprocessing of Virus-Based Therapeutics
inferred← citeshttps://doi.org/10.1007/s10529-024-03555-3openalex-snapshot
Application of aqueous two-phase extraction for separation and purification of various adeno-associated viruses
inferred← citeshttps://doi.org/10.1016/b978-0-443-15369-3.00030-4openalex-snapshot
Method development for large molecules IEX separations
inferred← citeshttps://doi.org/10.1016/j.ab.2023.115421openalex-snapshot
Separation of full and empty adeno-associated virus capsids by anion-exchange chromatography using choline-type salts
inferred← citeshttps://doi.org/10.1016/j.chroma.2022.463424openalex-snapshot
Selective reversed-phase high-performance liquid chromatography method for the determination of intact SARS-CoV-2 spike protein
inferred← citeshttps://doi.org/10.1016/j.copbio.2021.12.009openalex-snapshot
Leveraging rAAV bioprocess understanding and next generation bioanalytics development
inferred← citeshttps://doi.org/10.1016/j.tibtech.2025.02.016openalex-snapshot
Process and quality considerations for recombinant adeno-associated virus manufacturing platforms
inferred← citeshttps://doi.org/10.1016/j.trac.2023.117088openalex-snapshot
Chromatographic strategies for the analytical characterization of adeno-associated virus vector-based gene therapy products
inferred← citeshttps://doi.org/10.1016/j.xphs.2025.01.005openalex-snapshot
Comparative analysis of empty and full adeno-associated viruses under stress conditions by anion-exchange chromatography, analytical ultracentrifugation, and mass photometry
inferred← citeshttps://doi.org/10.1021/acs.analchem.1c02828openalex-snapshot
A Native Mass Spectrometry-Based Assay for Rapid Assessment of the Empty:Full Capsid Ratio in Adeno-Associated Virus Gene Therapy Products
inferred← citeshttps://doi.org/10.1021/jasms.2c00087openalex-snapshot
Surface Modified Nano-Electrospray Needles Improve Sensitivity for Native Mass Spectrometry
inferred← citeshttps://doi.org/10.1038/s41434-023-00398-xopenalex-snapshot
Large-scale purification of functional AAV particles packaging the full genome using short-term ultracentrifugation with a zonal rotor
inferred← citeshttps://doi.org/10.1089/hum.2022.013openalex-snapshot
Reverse-Phase Ultra-Performance Chromatography Method for Oncolytic Coxsackievirus Viral Protein Separation and Empty to Full Capsid Quantification
inferred← citeshttps://doi.org/10.1101/2021.07.06.451264openalex-snapshot
A native mass spectrometry-based assay for rapid assessment of the empty/full capsid ratio in AAV gene therapy products
inferred← citeshttps://doi.org/10.1208/s12249-025-03220-6openalex-snapshot
Integrated Analytical Techniques to Investigate the Effect of the Freezing/Thawing Cycles on the Non-replicating Recombinant Chimpanzee Adenovirus Viral Vector COVID-19 Vaccine
inferred← citeshttps://doi.org/10.3390/ph15010023openalex-snapshot
PCR-Based Analytical Methods for Quantification and Quality Control of Recombinant Adeno-Associated Viral Vector Preparations
inferred← citeshttps://doi.org/10.3390/vaccines9080823openalex-snapshot
Critical Assessment of Purification and Analytical Technologies for Enveloped Viral Vector and Vaccine Processing and Their Current Limitations in Resolving Co-Expressed Extracellular Vesicles
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