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Extensive Pyrosequencing Reveals Frequent Intra-Genomic Variations of Internal Transcribed Spacer Regions of Nuclear Ribosomal DNA

https://doi.org/10.1371/journal.pone.0043971

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What this work says

Outgoing claims: the papers this work cites, builds on, or argues with.

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Incoming claims: where this work appears in other papers’ reference lists and arguments.

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Congruence of chloroplast- and nuclear-encoded DNA sequence variations used to assess species boundaries in the soil microalga Heterococcus(Stramenopiles, Xanthophyceae)

inferred← citeshttps://doi.org/10.1186/1471-2148-14-153openalex-snapshot

Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli(family Hesperiidae) comprises three morphologically cryptic species

inferred← citeshttps://doi.org/10.1186/1471-2164-13-722openalex-snapshot

Next generation sequencing analysis reveals a relationship between rDNA unit diversity and locus number in Nicotiana diploids

inferred← citeshttps://doi.org/10.1186/s12862-015-0325-1openalex-snapshot

Intra-genomic variation in symbiotic dinoflagellates: recent divergence or recombination between lineages?

inferred← citeshttps://doi.org/10.1186/s12862-015-0416-zopenalex-snapshot

A genome-scale mining strategy for recovering novel rapidly-evolving nuclear single-copy genes for addressing shallow-scale phylogenetics in Hydrangea

inferred← citeshttps://doi.org/10.1186/s12870-017-0978-6openalex-snapshot

Evolutional dynamics of 45S and 5S ribosomal DNA in ancient allohexaploid Atropa belladonna

inferred← citeshttps://doi.org/10.1186/s13020-015-0062-9openalex-snapshot

Identification of Hippophae species (Shaji) through DNA barcodes

inferred← citeshttps://doi.org/10.1186/s13020-015-0071-8openalex-snapshot

Authentication of commercial processed Glehniae Radix (Beishashen) by DNA barcodes

inferred← citeshttps://doi.org/10.1186/s13020-022-00590-yopenalex-snapshot

Strategies for molecular authentication of herbal products: from experimental design to data analysis

inferred← citeshttps://doi.org/10.1186/s13020-022-00655-yopenalex-snapshot

DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances

inferred← citeshttps://doi.org/10.1186/s13020-024-00911-3openalex-snapshot

Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing

inferred← citeshttps://doi.org/10.1360/n052017-00204openalex-snapshot

DNA metabarcoding identification of prescription ingredients in traditional medicine Ruyi Jinhuang San

inferred← citeshttps://doi.org/10.1371/journal.pone.0066726openalex-snapshot

Compensatory Base Changes in ITS2 Secondary Structures Correlate with the Biological Species Concept Despite Intragenomic Variability in ITS2 Sequences – A Proof of Concept

inferred← citeshttps://doi.org/10.1371/journal.pone.0067818openalex-snapshot

Application of the ITS2 Region for Barcoding Medicinal Plants of Selaginellaceae in Pteridophyta

inferred← citeshttps://doi.org/10.1371/journal.pone.0069793openalex-snapshot

Characterization of Haemaphysalis flava (Acari: Ixodidae) from Qingling Subspecies of Giant Panda (Ailuropoda melanoleuca qinlingensis) in Qinling Mountains (Central China) by Morphology and Molecular Markers

inferred← citeshttps://doi.org/10.1371/journal.pone.0089769openalex-snapshot

Multiple ITS Copies Reveal Extensive Hybridization within Rheum (Polygonaceae), a Genus That Has Undergone Rapid Radiation

inferred← citeshttps://doi.org/10.1371/journal.pone.0094297openalex-snapshot

Deep-Sequencing Method for Quantifying Background Abundances of Symbiodinium Types: Exploring the Rare Symbiodinium Biosphere in Reef-Building Corals

inferred← citeshttps://doi.org/10.1371/journal.pone.0103003openalex-snapshot

ITS Polymorphisms Shed Light on Hybrid Evolution in Apomictic Plants: A Case Study on the Ranunculus auricomus Complex

inferred← citeshttps://doi.org/10.1371/journal.pone.0154631openalex-snapshot

DNA Barcoding of the Endangered Aquilaria (Thymelaeaceae) and Its Application in Species Authentication of Agarwood Products Traded in the Market

inferred← citeshttps://doi.org/10.1371/journal.pone.0157505openalex-snapshot

Large-Scale Monitoring of Plants through Environmental DNA Metabarcoding of Soil: Recovery, Resolution, and Annotation of Four DNA Markers

inferred← citeshttps://doi.org/10.1371/journal.pone.0160744openalex-snapshot

Contrasting Patterns of rDNA Homogenization within the Zygosaccharomyces rouxii Species Complex

inferred← citeshttps://doi.org/10.1371/journal.pone.0181491openalex-snapshot

Assessing intragenomic variation of the internal transcribed spacer two: Adapting the Illumina metagenomics protocol

inferred← citeshttps://doi.org/10.1371/journal.pone.0201711openalex-snapshot

Identification of three Daphne species by DNA barcoding and HPLC fingerprint analysis

inferred← citeshttps://doi.org/10.1371/journal.pone.0206851openalex-snapshot

An improved environmental DNA assay for bull trout (Salvelinus confluentus) based on the ribosomal internal transcribed spacer I

inferred← citeshttps://doi.org/10.1371/journal.pone.0251471openalex-snapshot

Molecular characterization of leaf spot caused by Alternaria alternata on buttonwood (Conocarpus erectus L.) and determination of pathogenicity by a novel disease rating scale

inferred← citeshttps://doi.org/10.1371/journal.pone.0282715openalex-snapshot

Investigating the use of pollen DNA metabarcoding to quantify bee foraging and effects of threshold selection

inferred← citeshttps://doi.org/10.1534/g3.116.036145openalex-snapshot

Using Next-Generation Sequencing for DNA Barcoding: Capturing Allelic Variation in ITS2

inferred← citeshttps://doi.org/10.1600/036364418x697382openalex-snapshot

DNA Sequence Variation among Conspecific Accessions of the Legume Coursetia caribaea Reveals Geographically Localized Clades Here Ranked as Species

inferred← citeshttps://doi.org/10.1600/036364422x16442668423347openalex-snapshot

Revised Taxonomy of theArctotisAnnual Clade (Arctotideae, Asteraceae) from Southern Africa: Integration of Molecular Phylogenetic and Morphological Evidence

inferred← citeshttps://doi.org/10.18174/463307openalex-snapshot

Next generation DNA sequencing based strategies; towards a new era for the traceability of endangered species and genetically modified organisms

inferred← citeshttps://doi.org/10.18699/vj20.640openalex-snapshot

Identification of species in the genus Nitraria L. (Nitrariaceae) based on nucleotide variability of nuclear ribosomal DNA

inferred← citeshttps://doi.org/10.20944/preprints202206.0090.v1openalex-snapshot

Ecotype Division and Chemical Diversity of Cynomorium songaricum from Different Geographical Regions

inferred← citeshttps://doi.org/10.2174/2210290601405010001openalex-snapshot

High Levels of Genetic Diversity within One Population of Rheum tanguticum on the Qinghai-Tibet Plateau have Implications for Germplasm Conservation

inferred← citeshttps://doi.org/10.2216/15-115.1openalex-snapshot

New insights into the morphology and phylogeny ofHeterosigma akashiwo(Raphidophyceae), with the description ofHeterosigma minor sp. nov.

inferred← citeshttps://doi.org/10.24247/ijasraug201816openalex-snapshot

DNA Barcoding for Differentiating the 11 Varieties of Musa Species

inferred← citeshttps://doi.org/10.30848/pjb2020-5(34)openalex-snapshot

Molecular diversity of halophilic fungi isolated from mangroves ecosystem of Miani Hor, Balochistan, Pakistan

inferred← citeshttps://doi.org/10.30901/2227-8834-2016-4openalex-snapshot

inferred← citeshttps://doi.org/10.3103/s0095452724060112openalex-snapshot

Intragenomic Polymorphism of the ITS1-5.8S-ITS2 Region in Invasive Species of the Genus Reynoutria

inferred← citeshttps://doi.org/10.3389/fphar.2020.00336openalex-snapshot

Tracing the Edible and Medicinal Plant Pueraria montana and Its Products in the Marketplace Yields Subspecies Level Distinction Using DNA Barcoding and DNA Metabarcoding

inferred← citeshttps://doi.org/10.3389/fphar.2021.607197openalex-snapshot

Biological Ingredient Analysis of Traditional Herbal Patent Medicine Fuke Desheng Wan Using the Shotgun Metabarcoding Approach

inferred← citeshttps://doi.org/10.3389/fphar.2025.1610301openalex-snapshot

The anticancer activity and mechanisms of She medicine herbs

inferred← citeshttps://doi.org/10.3389/fpls.2016.00467openalex-snapshot

The Chemical and Genetic Characteristics of Szechuan Pepper (Zanthoxylum bungeanum and Z. armatum) Cultivars and Their Suitable Habitat

inferred← citeshttps://doi.org/10.3389/fpls.2017.00926openalex-snapshot

Quality Control of the Traditional Patent Medicine Yimu Wan Based on SMRT Sequencing and DNA Barcoding

inferred← citeshttps://doi.org/10.3389/fpls.2022.852406openalex-snapshot

5S Ribosomal DNA of Genus Solanum: Molecular Organization, Evolution, and Taxonomy

inferred← citeshttps://doi.org/10.3389/fpls.2022.984825openalex-snapshot

Classification of 17 species Aegilops using DNA barcoding and SNPs, reveals gene flow among Aegilops biuncialis, Aegilops juvenalis, and Aegilops columnaris

inferred← citeshttps://doi.org/10.3390/ani12070926openalex-snapshot

Molecular Analysis of the Heterakis dispar Population in Domestic Geese Based on the ITS1-5.8rRNA-ITS2 Fragment

inferred← citeshttps://doi.org/10.3390/app7030195openalex-snapshot

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

inferred← citeshttps://doi.org/10.3390/books978-3-03928-367-5openalex-snapshot

Repetitive DNA Sequences

inferred← citeshttps://doi.org/10.3390/genes10050345openalex-snapshot

Integrative rDNAomics—Importance of the Oldest Repetitive Fraction of the Eukaryote Genome

inferred← citeshttps://doi.org/10.3390/genes14020480openalex-snapshot

Sequence Characterization of ITS Regions of Immortelle Helichrysum italicum (Roth) G. Don from the East Adriatic Coast

inferred← citeshttps://doi.org/10.3390/ijms27167351openalex-snapshot

Intragenomic and Interspecific Polymorphism of 35S rDNA Internal Transcribed Spacer 1 (ITS1) in Selenicereus and Related Species (Cactaceae: Cactoideae: Phyllocacteae)

inferred← citeshttps://doi.org/10.3390/molecules27133967openalex-snapshot

Ecotype Division and Chemical Diversity of Cynomorium songaricum from Different Geographical Regions

inferred← citeshttps://doi.org/10.3732/apps.1700079openalex-snapshot

Using Herbarium‐Derived DNAs to Assemble a Large‐Scale DNA Barcode Library for the Vascular Plants of Canada

inferred← citeshttps://doi.org/10.3897/mbmg.5.68155openalex-snapshot

Internal transcribed spacer primer evaluation for vascular plant metabarcoding

inferred← citeshttps://doi.org/10.3897/phytokeys.87.12774openalex-snapshot

Molecular phylogenetics of cool-season grasses in the subtribes Agrostidinae, Anthoxanthinae, Aveninae, Brizinae, Calothecinae, Koeleriinae and Phalaridinae (Poaceae, Pooideae, Poeae, Poeae chloroplast group 1)

inferred← citeshttps://doi.org/10.4236/cm.2014.54023openalex-snapshot

Molecular Identification of Chinese Materia Medica and Its Adulterants Using ITS2 and psbA-trnH Barcodes: A Case Study on Rhizoma Menispermi

inferred← citeshttps://doi.org/10.4238/2015.october.2.9openalex-snapshot

Unraveling systematic inventory of Echinops (Asteraceae) with special reference to nrDNA ITS sequence-based molecular typing of Echinops abuzinadianus

inferred← citeshttps://doi.org/10.4311/2016mb0146openalex-snapshot

First survey of the fungi from the Bakwena Cave in South Africa suggests low human disturbance

inferred← citeshttps://doi.org/10.6084/m9.figshare.25309999openalex-snapshot

Additional file 1 of Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing

inferred← citeshttps://doi.org/10.6084/m9.figshare.25310206openalex-snapshot

Additional file 2 of Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing

inferred← citeshttps://doi.org/10.7287/peerj.13884v0.1/reviews/1openalex-snapshot

Peer Review #1 of "A phylogenetic analysis of Bromus (Poaceae: Pooideae: Bromeae) based on nuclear ribosomal and plastid data, with a focus on Bromus sect. Bromus (v0.1)"

inferred← citeshttps://doi.org/10.7287/peerj.13884v0.1/reviews/2openalex-snapshot

Peer Review #2 of "A phylogenetic analysis of Bromus (Poaceae: Pooideae: Bromeae) based on nuclear ribosomal and plastid data, with a focus on Bromus sect. Bromus (v0.1)"

inferred← citeshttps://doi.org/10.7287/peerj.13884v0.2/reviews/1openalex-snapshot

Peer Review #1 of "A phylogenetic analysis of Bromus (Poaceae: Pooideae: Bromeae) based on nuclear ribosomal and plastid data, with a focus on Bromus sect. Bromus (v0.2)"

inferred← citeshttps://doi.org/10.7287/peerj.13884v0.2/reviews/2openalex-snapshot

Peer Review #2 of "A phylogenetic analysis of Bromus (Poaceae: Pooideae: Bromeae) based on nuclear ribosomal and plastid data, with a focus on Bromus sect. Bromus (v0.2)"

inferred← citeshttps://doi.org/10.7287/peerj.718v0.1/reviews/1openalex-snapshot

Peer Review #1 of "Intragenomic polymorphisms among high-copy loci: a genus-wide study of nuclear ribosomal DNA in Asclepias (Apocynaceae) (v0.1)"

inferred← citeshttps://doi.org/10.7287/peerj.718v0.1/reviews/2openalex-snapshot

Peer Review #2 of "Intragenomic polymorphisms among high-copy loci: a genus-wide study of nuclear ribosomal DNA in Asclepias (Apocynaceae) (v0.1)"

inferred← citeshttps://doi.org/10.7717/peerj.13884openalex-snapshot

A phylogenetic analysis of Bromus (Poaceae: Pooideae: Bromeae) based on nuclear ribosomal and plastid data, with a focus on Bromus sect. Bromus

inferred← citeshttps://doi.org/10.7717/peerj.718openalex-snapshot

Intragenomic polymorphisms among high-copy loci: a genus-wide study of nuclear ribosomal DNA in Asclepias (Apocynaceae)

inferred← citeshttps://doi.org/10.7872/crya.v36.iss1.2015.81openalex-snapshot

Comparison of Lichen-Forming Cyanobacterial and Green Algal Photobionts with Free-Living Algae

Editorial review for independent work. ICSAC — the Institute for Complexity Science and Advanced Computing — runs a structured editorial panel for researchers publishing outside journals, and publishes on CiteStamp rails. Submission is free and goes through ORCID: icsacinstitute.org/submit.